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Efficient production method of biological polysaccharide gellan gum

A biological polysaccharide and production method technology, applied in the field of microbial fermentation, can solve the problems of slow direct utilization of nitrogen source of beans, inability to obtain high-yield gellan gum, high production cost of gellan gum, etc., to achieve easy control and improve utilization rate , The effect of unit capacity increase

Inactive Publication Date: 2014-06-04
淄博迪森生物科技有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although there is a design in the prior art to directly use starch carbon source and bean nitrogen source to produce gellan gum, the yield of gellan gum is still low in actual production, so that the production cost of gellan gum is increased
The applicant finds in the process of realizing the present invention that there are two important reasons why the yield of gellan gum is produced by using starch carbon source and beans nitrogen source. The direct utilization rate of starch carbon source and bean nitrogen source is low, resulting in the inability to obtain high-yield gellan gum in fermentation production; second, the direct utilization of bean nitrogen source by bacteria is relatively slow, resulting in low fermentation production efficiency , adding too many ingredients such as inorganic salts, in a disguised form increases the cost of production raw materials

Method used

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  • Efficient production method of biological polysaccharide gellan gum
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Embodiment 1

[0035] Embodiments 1 to 4 are specific implementations of a high-efficiency production method of biopolysaccharide gellan gum of the present invention, wherein embodiment 1 is the best embodiment.

[0036] Example 1

[0037] In the fermentation culture, the fermentation broth is configured in the following steps:

[0038] Configure carbon source solution: in a 50-square fermenter, use cornstarch and tap water to configure a starch solution with a mass percentage of 8%;

[0039] Sterilization of carbon source solution: heat and sterilize the starch solution at 121°C for 30 minutes, and cool down to 28~33°C after the end;

[0040] Carbon source solution inoculation: add Xanthomonas culture solution to the starch solution, the volume ratio of Xanthomonas culture solution to starch solution is 4% (v / v), keep the temperature at 28~33°C, pass sterile air, Mix for 15 hours to obtain starch treatment solution;

[0041] Prepare nitrogen source mixed solution: mix soybean meal, cal...

Embodiment 2

[0051] In the fermentation culture, the fermentation broth is configured in the following steps:

[0052] Configure carbon source solution: in a 50-square fermenter, use starch and groundwater to configure a starch solution with a mass percentage of 8%;

[0053] Sterilization of carbon source solution: heat and sterilize the starch solution at 130°C for 15 minutes, and cool down to 28~33°C after the end;

[0054] Carbon source solution inoculation: add Xanthomonas culture solution to the starch solution, the volume ratio of Xanthomonas culture solution to starch solution is 4% (v / v), keep the temperature at 28~33°C, pass sterile air, Mix for 15 hours to obtain starch treatment solution;

[0055] Configure the nitrogen source mixed solution: mix soybean meal, calcium carbonate and groundwater in a temporary storage tank to prepare the nitrogen source mixed solution. The nitrogen source mixed solution contains 2% soybean meal and 0.8% calcium carbonate in terms of mass percenta...

Embodiment 3

[0064] In the fermentation culture, the fermentation broth is configured in the following steps:

[0065] Configure carbon source solution: in a 50-square fermenter, use starch and groundwater to configure a starch solution with a mass percentage of 6%;

[0066] Sterilization of carbon source solution: heat and sterilize the starch solution at 121°C for 30 minutes, and cool down to 28~33°C after the end;

[0067] Carbon source solution inoculation: add Xanthomonas culture solution to the starch solution, the volume ratio of Xanthomonas culture solution to starch solution is 4% (v / v), keep the temperature at 28~33°C, pass sterile air, Mix for 15 hours to obtain starch treatment solution;

[0068] Configure the nitrogen source mixed solution: mix soybean meal, calcium carbonate and groundwater in a temporary storage tank to prepare the nitrogen source mixed solution. The nitrogen source mixed solution contains 2% soybean meal and 0.8% calcium carbonate in terms of mass percenta...

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Abstract

The invention relates to an efficient production method of biological polysaccharide gellan gum, and belongs to the technical field of microbial fermentation. The method comprises the following steps: seed culture; fermental cultivation; extraction, drying and crushing; and obtaining of coarse gellan gum. The method is characterized in that a fermental culture solution in fermental cultivation is obtained by way of a pre-treatment of inoculating xanthomonas to a starch solution and carrying out an alkali treatment on a nitrogen source, so that the production speed and productivity of the gellan gum are improved. The efficient production method of the biological polysaccharide gellan gum provided by the invention has the advantages that the output and production rate of the gellan gum can be effectively increased, and the gellan gum can be produced by taking starch which is low in price as a carbon source and bean pulps which are low in price as a nitrogen source, so that the production cost is lowered, the unit capacity is increased by 30-35%, the unit cost is saved by about 15-40% and the sugar-gel conversion rate is increased to over 70%.

Description

technical field [0001] A high-efficiency production method of biological polysaccharide gellan gum belongs to the technical field of microbial fermentation. Background technique [0002] Gellan gum is another new microbial exopolysaccharide after xanthan gum, and its gel performance is superior to that of xanthan gum. Gellan gum is a high-molecular sugar compound composed of four sugar molecules, D-glucose, D-glucuronic acid, D-glucose, and L-rhamnose connected by glycosidic bonds. The first glucose molecule is Linked by β-1,4 glycosidic bonds. It is an extracellular polysaccharide colloid produced by aerobic fermentation with Sphingomonas pauciformis as the producing bacteria. It is a new type of gelling agent. The gel formed is rich in juice and has good flavor release Sex, with a mouth-melting taste. As a new type of microbial exopolysaccharide, gellan gum is widely used in food, medicine and industrial fields due to its acid resistance, alkali resistance, salt resista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04
Inventor 石岩昌陈健杜金锁王晓港王春华李苗苗司书锋朱永真尹丽华
Owner 淄博迪森生物科技有限责任公司
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