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Cladosporium cladosporioides and lignocellulolyticenzyme preparation

A technology of Cladosporium cladoides and lignocellulase, which is applied in the biological field, can solve the problems of lack of hydrolytic complex enzymes, etc., and achieve the effects of enhancing industrial operability, reducing temperature difference, and fully utilizing raw materials

Active Publication Date: 2015-05-06
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the existing commercialized lignocellulose hydrolysis compound enzyme preparations involves ligninase, and only a few cases of research involving laccase in ligninase have been reported in the literature. Enzyme system has not been reported
[0004] At the same time, the enzymatic hydrolysis temperature range of the existing lignocellulose hydrolysis compound enzyme is 35-65°C, and the hydrolysis compound enzyme suitable for high-efficiency saccharification of lignocellulose under medium and low temperature conditions is scarce, and the enzymatic hydrolysis temperature is below 30°C and contains lignin Enzymatic lignocellulose hydrolysis compound enzyme system is still blank

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] This example is used to illustrate the lignocellulosic enzyme preparation provided by the present invention.

[0050] 1L potato culture medium was mixed with 0.05kg wheat bran to obtain a nutrient solution, inoculated with Cladosporium cladoides (CGMCC No.9587) according to a 5% ratio, cultivated at a constant temperature of 30°C for 3 days, collected the fermented solution, and used 50% ( NH 4 ) 2 SO 4 Precipitate and dialyze, and the obtained product is spray-dried to make fermented dry powder.

[0051] Lignocellulosic enzyme preparation 1 was obtained by mixing fermented dry powder and xylanase (sourced from Aspergillus clavatus, purchased from Baoruyi Beijing Biotechnology Co., Ltd.) at a weight ratio of 1:2.

Embodiment 2

[0053] The lignocellulosic enzyme preparation was prepared by the same method as in Example 1, except that the xylanase was derived from Aspergillus niger (purchased from Genencor Bioengineering Co., Ltd.).

[0054] Lignocellulosic enzyme preparation 2 was obtained.

Embodiment 3

[0056] The lignocellulosic enzyme preparation was prepared in the same manner as in Example 1, except that the xylanase was derived from Trichoderma reesei (purchased from Novozymes Investment Co., Ltd.).

[0057] Lignocellulosic enzyme preparation 3 was obtained.

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Abstract

The invention relates to cladosporium cladosporioides preserved at the China General Microbiological Culture Collection Center on September 4, 2014, wherein the preservation number is CGMCC NO.9587. The invention further provides an enzyme preparation containing the cladosporium cladosporioides and application of the enzyme preparation in saccharification of a lignocelluloses raw material. The lignocellulolyticenzyme preparation provided by the invention is capable of finishing the saccharification treatment process of the lignocelluloses raw material at a relatively low temperature, is high in polysaccharide yield, low in energy consumption, especially suitable for large-scale industrial application in the field of cellulosic ethanol fermentation production; and the raw materials are relatively fully utilized.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a Cladosporium cladoides and lignocellulosic enzyme preparation. Background technique [0002] The conversion of lignocellulosic raw materials such as agricultural and forestry wastes and energy plants to produce fuel ethanol is of great significance to alleviate the energy and environmental crisis. At present, the high cost of cellulosic ethanol is still the main factor limiting its large-scale industrial production and application. Among them, enzyme cost accounts for 50-60% of the total cost, followed by energy cost. A single type of lignocellulosic enzymes can no longer meet the needs of industry, and the synergistic effect of multiple lignocellulosic enzymes is needed to increase the yield of fermentable sugars from lignocellulosic raw materials while reducing the amount of enzymes used. However, the reported saccharification efficiency of lignocellulose hydrolysis com...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/14C12P7/10C12R1/645
CPCC12P7/10C12P19/14C12N1/145C12R2001/645Y02E50/10
Inventor 袁红莉季蕾杨金水
Owner CHINA AGRI UNIV
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