Cladosporium cladosporioides and lignocellulolyticenzyme preparation

A technology of Cladosporium cladoides and lignocellulase, which is applied in the biological field, can solve the problems of lack of hydrolytic complex enzymes, etc., and achieve the effects of enhancing industrial operability, reducing temperature difference, and fully utilizing raw materials

Active Publication Date: 2015-05-06
CHINA AGRI UNIV
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the existing commercialized lignocellulose hydrolysis compound enzyme preparations involves ligninase, and only a few cases of research involving laccase in ligninase have been reported in the literature. Enzyme system has not been reported
[0004] At the same time, the enzymatic hydrolysis temperature range of the existing lignoce

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0048] Example 1

[0049] This example is used to illustrate the lignocellulase preparation provided by the present invention.

[0050] Mix 1L of potato culture medium with 0.05kg wheat bran to obtain a culture solution, inoculate Cladosporium cladosporium (CGMCC No. 9587) at a ratio of 5%, cultivate at a constant temperature of 30°C for 3 days, collect the fermentation broth, and use 50% ( NH 4 ) 2 SO 4 After precipitation and dialysis, the obtained product is spray-dried to produce fermented dry powder.

[0051] The fermented dry powder and xylanase (source from Aspergillus clavatus, purchased from Baoruyi Beijing Biotechnology Co., Ltd.) were mixed in a weight ratio of 1:2 to obtain lignocellulase preparation 1.

Example Embodiment

[0052] Example 2

[0053] The lignocellulase preparation was prepared by the same method as in Example 1, except that the xylanase was derived from Aspergillus niger (purchased from Genencor Bioengineering Co., Ltd.).

[0054] Obtain lignocellulase preparation 2.

Example Embodiment

[0055] Example 3

[0056] The lignocellulase preparation was prepared by the same method as in Example 1, except that the xylanase was derived from Trichoderma reesei (purchased from Novozymes Investment Co., Ltd.).

[0057] Obtain lignocellulase preparation 3.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to cladosporium cladosporioides preserved at the China General Microbiological Culture Collection Center on September 4, 2014, wherein the preservation number is CGMCC NO.9587. The invention further provides an enzyme preparation containing the cladosporium cladosporioides and application of the enzyme preparation in saccharification of a lignocelluloses raw material. The lignocellulolyticenzyme preparation provided by the invention is capable of finishing the saccharification treatment process of the lignocelluloses raw material at a relatively low temperature, is high in polysaccharide yield, low in energy consumption, especially suitable for large-scale industrial application in the field of cellulosic ethanol fermentation production; and the raw materials are relatively fully utilized.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a Cladosporium cladoides and lignocellulosic enzyme preparation. Background technique [0002] The conversion of lignocellulosic raw materials such as agricultural and forestry wastes and energy plants to produce fuel ethanol is of great significance to alleviate the energy and environmental crisis. At present, the high cost of cellulosic ethanol is still the main factor limiting its large-scale industrial production and application. Among them, enzyme cost accounts for 50-60% of the total cost, followed by energy cost. A single type of lignocellulosic enzymes can no longer meet the needs of industry, and the synergistic effect of multiple lignocellulosic enzymes is needed to increase the yield of fermentable sugars from lignocellulosic raw materials while reducing the amount of enzymes used. However, the reported saccharification efficiency of lignocellulose hydrolysis com...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14C12P19/14C12P7/10C12R1/645
CPCC12P7/10C12P19/14C12N1/145C12R2001/645Y02E50/10
Inventor 袁红莉季蕾杨金水
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap