Escherichia coli fermentation medium and fermentation technology for producing succinic acid

A fermentation medium, Escherichia coli technology, applied in fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of reducing the production cost of succinic acid, low sugar-acid conversion rate, long fermentation time, etc., to achieve rich carbon sources and Effects of nitrogen source, shortened culture time, and increased cell density

Inactive Publication Date: 2018-10-02
山东兰典生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Specifically, the technical problem to be solved by the present invention is: provide a kind of Escherichia coli fermentation medium and fermentation process for producing succinic acid, to solve the problem of long total fermentation time and low sugar-acid conversion rate of existing succinic acid microbial fermentation process And the problem of low product concentration, thereby reducing the production cost of succinic acid

Method used

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  • Escherichia coli fermentation medium and fermentation technology for producing succinic acid
  • Escherichia coli fermentation medium and fermentation technology for producing succinic acid

Examples

Experimental program
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Effect test

Embodiment 1

[0041] After Escherichia coli (E.coli) was activated, it was transferred into a primary seed tank (1m 3 ) in the seed medium, the temperature is controlled at 31°C, the air volume is controlled at 0.85vvm, the DO is controlled at 25%, the stirring speed is 350r / min, the tank pressure is 0.05Mpa, and the aerobic fermentation is 11h;

[0042] Escherichia coli (E.coli) was transferred to the secondary seed tank (20m 3 ) in the seed medium, the temperature is controlled at 31°C, the air volume is controlled at 1.1vvm, the DO is controlled at 15%, the stirring speed is 200r / min, the tank pressure is 0.05Mpa, and the aerobic fermentation is 6h;

[0043] The seed medium components in the primary seed tank and the secondary seed tank are: glucose: 20g / L, peptone: 10g / L, KH 2 PO 4 : 1g / L, MgSO 4 ·7H 2 O: 0.5g / L, add 5mol / LNaOH external source flow to adjust the pH value of the culture medium to 7.0, the culture medium in the primary seed tank is adjusted to half the volume of the p...

Embodiment 2

[0047] The only difference between this embodiment and embodiment 1 is that the formula ratio of the fermentation medium in the fermenter is adjusted.

[0048] After Escherichia coli (E.coli) was activated, it was transferred into a primary seed tank (1m 3 ) in the seed medium, the temperature is controlled at 31°C, the air volume is controlled at 0.85vvm, the DO is controlled at 25%, the stirring speed is 350r / min, the tank pressure is 0.05Mpa, and the aerobic fermentation is 11h;

[0049] Escherichia coli (E.coli) was transferred to the secondary seed tank (20m 3 ) in the seed medium, the temperature is controlled at 31°C, the air volume is controlled at 1.1vvm, the DO is controlled at 15%, the stirring speed is 200r / min, the tank pressure is 0.05Mpa, and the aerobic fermentation is 6h;

[0050] The seed medium components in the primary seed tank and the secondary seed tank are: glucose: 20g / L, peptone: 10g / L, KH 2 PO 4 : 1g / L, MgSO 4 ·7H 2 O: 0.5g / L, add 5mol / LNaOH ext...

Embodiment 3

[0054] The only difference between this embodiment and embodiment 1 is that the formula ratio of the fermentation medium in the fermenter is adjusted.

[0055] After Escherichia coli (E.coli) was activated, it was transferred into a primary seed tank (1m 3 ) in the seed medium, the temperature is controlled at 31°C, the air volume is controlled at 0.85vvm, the DO is controlled at 25%, the stirring speed is 350r / min, the tank pressure is 0.05Mpa, and the aerobic fermentation is 11h;

[0056] Escherichia coli (E.coli) was transferred to the secondary seed tank (20m 3 ) in the seed medium, the temperature is controlled at 31°C, the air volume is controlled at 1.1vvm, the DO is controlled at 15%, the stirring speed is 200r / min, the tank pressure is 0.05Mpa, and the aerobic fermentation is 6h;

[0057] The seed medium in the primary seed tank and the secondary seed tank is: glucose: 20g / L, peptone: 10g / L, KH 2 PO 4 : 1g / L, MgSO 4 ·7H 2 O: 0.5g / L, add 5mol / LNaOH external source...

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Abstract

The invention relates to an escherichia coli fermentation medium and a fermentation technology for producing succinic acid. A formula of the medium comprises 1.4-1.6g/dL corn steep liquor dry powder,0.5-2.5g/L yeast extract, 0.2-1.4g/L MgSO4, 2.5-4.5g/L (NH4)2HPO4, 0.6-2.0g/L C5H11NO2.HCl, 0.2-1.0g/L KCl, 50-60mg/L CuSO4, and 35-50mg/L FeSO4.7H2O; a pH of the medium is 6.5-6.8; and a required volume is reached by distilled water. The fermentation technology comprises the steps of taking escherichia coli as a fermentation strain, and performing aerobic enrichment in a primary seeding tank anda secondary seeding tank, and anaerobic fermentation in a fermentation tank to form succinic acid, wherein a formula of the fermentation medium is the above formula. According to the medium and the technology, the total fermentation cost can be lowered by 22%.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to an Escherichia coli fermentation medium and a fermentation process for producing succinic acid. Background technique [0002] Succinic acid, also known as succinic acid, is an important dicarboxylic acid that can synthesize a variety of complex organic compounds and is widely used in the fields of medicine, food, synthetic plastics, and biodegradable materials. [0003] At present, the production process of chemical preparation of succinic acid is relatively mature, but there are disadvantages such as high energy consumption, large pollution, low yield and purity, and dependence on petrochemical resources, which inhibit its development potential as a bulk chemical. [0004] Compared with traditional chemical synthesis methods, microbial fermentation processes utilize renewable biomass resources and CO 2 As a raw material, it not only has low energy consumption and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12R1/19
CPCC12P7/46
Inventor 刘振龙高金龙丁占龙
Owner 山东兰典生物科技股份有限公司
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