Method for producing L-ornithine by microorganism fermentation

A technology of microbial fermentation and ornithine, applied in the field of microbial fermentation technology, can solve the problems of difficulty in large-scale production, high cost, and limiting the wide application of L-Orn.

Inactive Publication Date: 2008-12-17
上海聚瑞生物技术有限公司
View PDF1 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The enzyme (cell) method also uses expensive L-Arg as raw material, which is expen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing L-ornithine by microorganism fermentation
  • Method for producing L-ornithine by microorganism fermentation
  • Method for producing L-ornithine by microorganism fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0076] The present invention will be further described below by the examples, but the examples do not limit the protection scope of the present invention.

[0077] 1. Strain selection

[0078] (1) Strains

[0079] The starting strain is Corynebaetenom glutamicam preserved in this laboratory

[0080] (2) Culture medium and mutagenesis method

[0081] 1. The composition of the complete medium (A)

[0082] 1% beef extract; 1% peptone; 0.5% yeast extract; 0.5% NaCl; PH7.0 (PH6.4-8.0 test paper produced by Shanghai Reagent No. 4 Factory); add 2.0% agar powder when preparing solid medium.

[0083] 2. The composition of basic medium (B)

[0084] Glucose: 1%; (NH 4 ) 2 SO 4 : 0.3%; KH 2 PO 4 : 0.1%; K 2 HPO 4 ·3H 2 O: 0.2%; MgSO 4 ·7H 2 O: 0.05%; FeSO 4 ·7H 2 O: 0.002%; MgSO 4 0.002%, V B1 . HCl 200 μg / L, V H 50 μg / L, PH7.0~7.2 (adjusted with NaOH); agar powder 1.6%.

[0085] 3. Preparation of 1 / 30M phosphate buffer solution (PH7.0)

[0086] Liquid A: Na2 HPO 4 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for producing L-ornithine by microbial fermentation, namely, using Corynebacterium glutamate to obtain the strains of CS-189(cit<(-)>+SG<r>) by chemical treatment, and obtain the L-ornithine hydrochloride by fermentation, culture solution micro-filtration by a ceramic membrane, ion exchange resin and extraction by a hydrothermal crystallization method with decompression concentration. The strains used in the method are auxotrophy resistant to sulfaguanidine, which significantly enhances acid yield by fermentation. Meanwhile, aiming at the problem that the solubility of the L-ornithine hydrochloride in water is extremely difficult, the hydrothermal crystallization method is adopted to obtain better extraction rate under the condition that flammable and explosive alcohol is not used. The method simplifies processes and reduces extraction cost.

Description

technical field [0001] The invention relates to a microbial fermentation process, in particular to a method for producing L-ornithine by microbial fermentation. Background technique [0002] 1. Physicochemical properties, physiological functions and application in medicine of L-Ornithine (L-Orn). [0003] L-Orn is an amino acid that people are not familiar with. It is not a component of protein, but it is an amino acid that is ubiquitous in nature and also exists in the human body. It is a component of bacterial cell membranes and polypeptide antibiotics; food contains L- Orn, especially in clam shells, the content is higher, and clam shell juice is a well-known substance that is beneficial to the liver. The scientific name of L-Orn is α, δ-diaminovaleric acid, which was discovered by Jeff in 1877 in the hydrolyzate of bird urine fed with benzoic acid, so it is named ornithine, and its molecular formula is C 5 h 12 N 2 o 2 , the molecular weight is 132.16, and the struc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P13/10C12N1/20C12R1/15
Inventor 李炜徐虹李象洪
Owner 上海聚瑞生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap