32 results about "Sulfaguanidine" patented technology

The invention discloses streptomyces sp. for producing epsilon-polylysine and an application of the streptomyces sp., and belongs to the field of biotechnology. A streptomyces albulus (streptomyces albulus) M-Z18 bacterial strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO: M 2019589. The streptomyces albulus M-Z18 bacterialstrain can quickly grow on a culture medium using succinic acid as a carbon source, and has the capacity for high efficiency synthesis of epsilon-polylysine having sulfaguanidine resistance. The bacterial strain is subjected to fed-batch fermentation under a pH impact strategy, the accumulation concentration of the epsilon-polylysine can achieve 54.7g / L, the production efficiency can achieve 6.83g / L / d, and the streptomyces sp. is an epsilon-polylysine industrial production bacterial strain having great potential.

This invention refer to a method that using backstreaming technology to produce epsilon -poly-L-diaminocaproic acid. It uses blanc Streptomyces that screened from soil to fermentepsilon -poly-L-diaminocaproic acid[resist to S-(2-aminoethyl)-L-cysteine(abbreviate AEC below), Glycine, sulfaguanidine],then make the penetrated liquid from later stage of extracted procession by periodic duty,to fermentepsilon -poly-L-diaminocaproic acid; the penetrated liquid from later stage of extracted procession can be used for next elution. Decolorize the broth by negion exchange resin. This invention has changed the procedure of producing technology.Not only raise the yield ofepsilon -poly-L-diaminocaproic acid, save cost, but also reduce exhaustion of ferment liquid waste and hydrochloric acid liquid waste in elution, lessen environmental pollution; The decolor effect is enhanced and the lose is reduced becouse of decoloring the broth by negion exchange resin.

The invention relates to a novel method for increasing the yield of epsilon-poly-L-lysine, comprising the following steps: adopting Streptomyces diastatochromogenes or Streptomyces albulus which has resistance to S-(2 amino ethyl)-L- cysteine (called AEC for short), glycin and sulfaguanidine), and carrying out the fermentation production of the epsilon-poly-L-lysine through adding mixed solution of L-lysine, glucose and (NH4)2SO4 in a fed-match mode. The novel method can increase the yield by 25-50 percent than the process of with out the fed batch of L-lysine. The invention changes the method of the original production process, obviously increases the yield of the epsilon-poly-L-lysine, lowers the cost, and can be used for industrial scale fermentation.

The invention relates to a drug for treating piglet diarrhea and a using method. The drug for treating the piglet diarrhea is prepared from a drinking additive and a feed additive, wherein the drinking additive is prepared from, by weight, 70-90 g of fig leaves, 20-40 g of mulberry leaves, 30-50 g of hawthorn leaves, 20-40 g of loquat leaves, 20-40 g of tea leaves, 10-20 g of dried ginger, 10-15 g of brown sugar, 1-2 g of oxytetracycline, 2-4 g of baking soda and 1-2 g of sulfaguanidine, and the feed additive is prepared from, by weight, 30-50 g of white sugar, 20-40 g of garlic, 10-20 g of rhizoma coptidis and 2-5 g of mequindox. According to the drug for treating the piglet diarrhea and the using method, the drinking additive and the feed additive are cooperatively used to treat the piglet diarrhea from drinking water and feed and balance and regulate the physiological status of piglets, the treatment effect is good.

It is an L-leucine high-yield bacterium and the production of L-leucine with invoice method, belonging to biological engineering field. The invented bacterial is a corynebacterium glutamicum JH-27, using corynebacterium glutamicum ATCC13032 as starting strain, mutagenizing separately with diethyl sulfate, nitrosoguanidine and ultraviolet, adding separately analogue of branched chain amino acid into minimum medium and sulfaguanidine into complete medium and breeding to prepare, being an L-leucine high-yield bacterium resistant sulfaguanidine and branched chain amino acid, compared with starting strain, producing L-leucine with the strain and invoice method, it expresses the ability of accumulation of highly qualified L-leucine. Shaking flask and culturing JH-27 for 60 hours, the yield of L-leucine is 30-32 g / l. Fermenting in fermenter of 5L for 55 hours, the yield of L-leucine is 40g / l.

The invention belongs to the technical field of breeding of bamboo rats, and in particular discloses a medicine for preventing and treating enterogastritis in bamboo rats and a preparation method of the medicine. The medicine for preventing and treating enterogastritis in the bamboo rats is mainly prepared by the following materials through baking, extracting, mixing and drying: 10-15 parts of bentonite, 5-9 parts of silicon dioxide, 2-5 parts of sulfaguanidine, 1-5 parts of potassium clavulanate, 1-3 parts of a yeast, 5-10 parts of cherokee rose fruit, 3-8 parts of tea leaves, 3-8 parts of fructus crataegi, 2-8 parts of flos chrysanthemi and 1-5 parts of herba patriniae. The medicine for preventing and treating enterogastritis in bamboo rats provided by the invention, in accordance with principles of cooperative matching and assistant matching, combines chemical medicines and natural Chinese herbal medicines; the medicine mainly functions as diminishing inflammation and killing bacteria, clearing way heat and toxic materials, assisted by effects of clearing intestines and checking diarrhea; the medicine can be used for effectively preventing and treating the enterogastritis in bamboo rats and bring about benefits for healthy growth of the bamboo rats; in addition, the preparation method is simple; and various raw materials can be effectively combined.

The invention relates to a spray for dry cleaning woolen clothes and a preparation method thereof. The spray contains the following components in parts by mass: 10-28 parts of spermaceti, 5-14 parts of tea saponin, and 5-14 parts of potassium stearate , 2-8 parts of sodium alkylbenzene sulfonate, 1-3.5 parts of sulfaguanidine, 5-15 parts of silicone oil, 5-13 parts of polyacrylamide, 0.5-2.5 parts of polydimethylsiloxane, 0.5-2 parts of lotus essential oil Parts, 18-36 parts of ethanol, 80-145 parts of distilled water. The spray provided by the invention has good decontamination effect, can remove various stains on the woolen clothes, kill bacteria on the clothes, and can also remove peculiar smells on the clothes.

The invention discloses an application of an electrochemical immunosensor in the aspect of multi-residue detection of sulfonamides. According to the application, firstly, the surface of a glassy carbon electrode is modified with polysulfadiazine with an in-situ electrochemical polymerization method, secondly, detection for four sulfonamides including sulfadiazine, sulfamethazine, sulfacetamide andsulfaguanidine is realized on the basis of cross-immune response of sulfadiazine antibodies and sulfonamides, wherein the limit of detection is in a range of 0.06-0.15 ng / mL, and linear range is 0.1-1500 ng / mL or 0.3-500 ng / mL.

The invention discloses a strain of streptomyces producing ε-polylysine and its application, belonging to the technical field of bioengineering. The Streptomyces albulus strain (Streptomyces albulus) M-Z18 of the present invention has been deposited in the China General Microorganism Collection and Management Center on July 29, 2019, and the preservation number is CGMCC NO: M 2019589. The Streptomyces albicans M-Z18 strain of the present invention can grow rapidly on a medium with succinic acid as a carbon source, and has the ability to efficiently synthesize ε-polylysine with sulfaguanidine resistance. The strain of the present invention carries out fed-batch fermentation under the pH shock strategy, the cumulative concentration of ε-polylysine can reach 54.7g / L, and the production efficiency can reach 6.83g / L / d, which is a strain of ε-polylysine with great potential Polylysine industrial production strain.

The invention discloses bio-based antibacterial coating and a preparation method thereof. The bio-based antibacterial coating is characterized by being prepared from the following components: an antibacterial macromolecule, waterborne epoxy emulsion, a de-foaming agent, an antioxidant, a waterborne curing agent and de-ionized water, wherein the antibacterial macromolecule is prepared through covalent bonding of sulfaguanidine, DL-tartaric acid and glycitin; then the antibacterial macromolecule is grafted on an epoxy resin matrix to form the bio-based antibacterial coating. The bio-based antibacterial coating prepared by the method integrates sterilization and bacterium inhibition and has relatively good antibacterial performance, stable chemical performance and relatively good heat-resisting performance; and the defect that a coating layer becomes invalid, caused by a series of problems of poor heat-resisting performance of traditional bio-based antibacterial coating, can be overcome.

The invention discloses a chemically modified electrode for sensitive detection of sulfaguanidine and a preparation method and application thereof. The chemically modified electrode is a high-electroactivity AgPt@MXene nanocomposite modified glassy carbon electrode, and relates to the field of electroanalytical chemistry and electrochemical sensors. The AgPt@MXene heterojunction nanocomposite hasa large specific surface area and high catalytic activity, and is capable of enhancing the enrichment amount of the sulfaguanidine in the detection mediums, improving the sensitivity of the measurement, effectively accelerating the electron transfer and improving the electrochemical response signal. The prepared chemically modified electrode is good in selectivity, simple, low in cost and good instability, is suitable for on-site detection, and is successfully applied to the accurate detection of the sulfaguanidine in animal-derived foods.

The invention discloses a kit for nucleic acid extraction, the kit comprises a nucleic acid extraction component, the nucleic acid extraction component comprises guanidine salt, a surfactant, a buffer component and a chelating agent; the guanidine salt is one or more of sulfaguanidine, guanidine hydrochloride, guanidine cyanate, guanidine carbonate or guanidine nitrate; the surfactant is one or more of stearic acid, sodium dodecyl benzene sulfonate, quaternization compound, lecithin, alkyl glucoside, fatty glyceride, fatty acid sorbitan or polysorbate; and the buffer component is phosphate, Tris, carbonate or acetate. According to the nucleic acid extraction kit provided by the embodiment of the invention, after the sample is extracted by the nucleic acid extraction reagent, the impurity content can be effectively reduced, background enzymes are removed to prevent product degradation, meanwhile, a target product is protected, and the extraction efficiency is remarkably improved.

The invention discloses a detection method for malachite green in aquatic products. The detection method comprises experiment steps of preparing a magnetic molecularly imprinted polymer, establishinga method based on an aptamer and ferroferric oxide mimic enzyme catalysis and the like. According to the detection method, firstly, a dopamine polymerization method is used for preparing a malachite green (MG) magnetic molecularly imprinted polymer (MG-MMIPs); trace MG in a sample is adsorbed by utilizing the MG-MMIPs, so that the aims of enriching a target object and removing a complicated mediumare realized; then after enriched MG is desorbed, a detection method of the aptamer and ferroferric oxide mimic enzyme catalysis is used for detecting. The method has the sensitivity of 5 nmol L<-1>and the lowest detection limit reaches 3.6 nmol L<-1>. A specific experiment aiming at two structural analogues (including brilliant green and crystal violet) of MG and other bacteriostatic drugs (including sulfaguanidine, sulphanilamide, oxytetracycline hydrochloride and chloramphenicol) shows that the method has good selectivity in MG detection.

The invention discloses a medicine premix for treating and preventing donkey primary enteritis and digestive diarrhea and a preparation method thereof. The medicine premix is mainly composed of the following components in parts by weight: 550-800 parts of sweet potato leaves, 360-500 parts of bean cakes, 130-300 parts of mung bean hulls, 130-200 parts of sugarcane dry straw powder, 5-10 parts of afeed additive, 0.2-0.5 part of sulfaguanidine, 0.1-0.2 part of aspartame, 0.03-0.04 part of white camphor oil, 1-1.3 parts of table salt, 0.03-0.04 part of slow-release potassium, 20-30 parts of diatomite, 15-40 parts of a filler and 0.1-0.2 part of lubricating powder. The medicine premix can effectively prevent and treat enteritis diseases, digestive diarrhea and other problems, has a certain health-care function on donkeys, and can improve the immunocompetence of donkeys.