Method for preparing ethanol from cellulose-containing raw material

A technology of cellulose and cellulase, applied in the direction of microorganism-based methods, biochemical equipment and methods, biofuels, etc., can solve the problems of low ethanol yield, achieve increased ethanol yield, increase yield, and improve equipment The effect of utilization

Active Publication Date: 2014-05-14
COFCO NUTRITION & HEALTH RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of the low yield of ethanol produced by the existing method for preparing ethano

Method used

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  • Method for preparing ethanol from cellulose-containing raw material
  • Method for preparing ethanol from cellulose-containing raw material

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0061] Example 1

[0062] This example is used to illustrate the method of the present invention for preparing ethanol using cellulose-containing raw materials.

[0063] (1) Pretreatment of cellulose-containing raw materials

[0064] Cut 1000 kg of corn stalks (water content 10% by weight) without impurities into small sections not exceeding 1.2 cm × 0.5 cm × 1.0 cm, maintain a pressure of 1.0 MPa at 180°C for 5 minutes, then release the pressure to complete the steam explosion . A total of 1,800 kg of steam explosion bankruptcy (water content of 50% by weight) was obtained.

[0065] Determination of the total weight of cellulose and total weight of hemicellulose in the solid steam explosion product:

[0066] Take 10 grams of the above-mentioned steam explosion bankruptcy and dry it at 45°C to a constant weight of 5 grams, weigh 300.0 mg of the dried steam explosion bankruptcy, and place it in a 100 ml dry Erlenmeyer flask weighing 80 grams. Add 3.00 ml of a 72% by weight sulfuric ac...

Example Embodiment

[0076] Example 2

[0077] This example is used to illustrate the method of the present invention for preparing ethanol using cellulose-containing raw materials.

[0078] Ethanol was prepared according to the method of Example 1, except that in the enzymatic hydrolysis step (2), in the main enzymatic hydrolysis stage, the steam explosion product was adjusted to a pH value of 5, and after heating to 52°C, the main enzyme was continuously added In the enzymatic hydrolysis tank at the top of the hydrolysis stage, the amount of the steam explosion bankruptcy added is 25 kg / hour, and the cellulase is continuously added to the enzymatic hydrolysis tank at the first enzymatic hydrolysis stage in the main enzymatic hydrolysis stage. When the enzymatic hydrolysis product in the first enzymatic hydrolysis tank starts to flow into the enzymatic hydrolysis tank of the second enzymatic hydrolysis stage connected with the first one, and at 52°C, the cellulase is continuously added to the enzymati...

Example Embodiment

[0081] Example 3

[0082] This example is used to illustrate the method of the present invention for preparing ethanol using cellulose-containing raw materials.

[0083] Ethanol was prepared according to the method of Example 1, except that in the enzymatic hydrolysis step (2), in the main enzymatic hydrolysis stage, the steam explosion product was adjusted to a pH value of 5, and after heating to 52°C, the main enzyme was continuously added In the first enzymatic hydrolysis tank in the hydrolysis stage, the amount of the steam explosion bankruptcy added is 26.47 kg / h. First, the cellulase is continuously added to the enzymatic hydrolysis tank in the first enzymatic hydrolysis stage in the main enzymatic hydrolysis stage. When the enzymatic hydrolysis product in the first enzymatic hydrolysis tank starts to flow into the enzymatic hydrolysis tank of the second enzymatic hydrolysis stage connected with the first one, and at 52°C, the cellulase is continuously added to the enzymatic ...

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Abstract

The invention discloses a method for preparing ethanol from a cellulose-containing raw material, which comprises the following steps: in the presence of water, mixing a cellulose-containing raw material with enzyme, carrying out enzymolysis on the cellulose-containing raw material to obtain an enzymolysis product, and fermenting the enzymolysis product. The enzymolysis comprises a main enzymolysis stage and an intermitted enzymolysis stage after the main enzymolysis stage which are continuously performed, wherein in the main enzymolysis stage, proper amounts of the cellulose-containing raw material and enzyme are continuously added into an enzymolysis tank so that the monosaccharide content in the enzymolysis product in the main enzymolysis stage is 50-100 g/L, and the enzymolysis products of the main enzymolysis stage respectively and continuously flow into enzymolysis tanks of the subsequent stage to perform intermitted enzymolysis until the monosaccharide content in the enzymolysis products in the enzymolysis tanks in the subsequent stage at the enzymolysis end point is greater than or equal to 105 g/L. The method can effectively enhance the saccharide conversion rate of the raw material, and therefore, greatly enhances the yield of ethanol.

Description

technical field [0001] The invention relates to a method for preparing ethanol from cellulose-containing raw materials. Background technique [0002] The prior art method for preparing ethanol from cellulose-containing raw materials, such as straw, mainly includes three steps: 1. pretreatment of cellulose-containing raw materials, 2. enzymatic hydrolysis of cellulose, and 3. fermentation of sugar to produce ethanol. [0003] The cellulose in the cellulose-containing raw material after pretreatment is exposed, and the reaction of being hydrolyzed by cellulase after mixing with cellulase is usually called enzymatic hydrolysis reaction, and can also be called saccharification reaction. The main product of enzymatic hydrolysis is Monosaccharide. Cellulose can be converted into glucose under the action of cellulase, and hemicellulose can be converted into monosaccharides such as xylose under the action of hemicellulase. Both glucose and xylose can be used for fermentation to pro...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P39/00C12R1/865C12R1/84
CPCY02E50/10
Inventor 魏拥辉李春玲刘文信孙长征吴杨宋思琦任玲沈乃东
Owner COFCO NUTRITION & HEALTH RES INST
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