50 results about "Multidimensional liquid chromatography" patented technology

Methods and apparatus for characterizing a polymer sample and in preferred embodiments, libraries of polymer samples, in a comprehensive, directly-coupled multi-dimensional liquid chromatography system are disclosed. The first and second dimensions are preferably high-performance liquid chromatography dimensions, such as for example, a first dimension adapted for determining composition (e.g. adapted for mobile-phase gradient elution chromatography, including reverse phase chromatography, adsorption chromatography and the like), and a second dimension adapted for determining molecular weight or particle size (e.g., adapted for size exclusion chromatography, including gel permeation chromatography).

The invention relates to a multidimensional liquid phase chromatography separation system and separation method for protein separation. The multidimensional liquid phase chromatography separation system for protein separation comprises a mobile phase storage tank, a first liquid delivery device, a second liquid delivery device, a first sampling device, a second sampling device, a separation device, a collection and storage device, at least two liquid discharge devices and a flow path switch. When implementing every dimension of liquid phase chromatography separation, the collection and storage device collects a target intermediate fraction for the next liquid phase chromatography separation step and stores the target intermediate fraction in the collection and storage unit; and in the second or more dimension liquid phase chromatography separation process, all the target intermediate fraction from the collection and storage unit is mixed with the mobile phase through a sampling mixer of the second sampling device, and the first liquid delivery device and the second liquid delivery device are utilized to adjust and meter the quantity of the delivered mobile phase, so that the concentration of the eluting agent in the sampled mixed solution is lower than the concentration of the critical migration eluting agent for all the target proteins to be preserved in the separation process in the target intermediate fraction.

A multi-dimensional separation system having parallel traps for effluent from prior separation dimension and parallel latter separation columns, the latter columns being coupled to the traps. At least one trap enriches components of effluent while at least one other trap is releasing trapped components to a detector, which may be a mass spectrometer. Internal standards may be provided, as in a release solvent, for the calibration of one of the chromatographic columns and the detection system. The system may comprise a multiple channel selector for multiple streams, wherein all of the streams flow at the same time.

The invention provides a new method for double quantifying the protein group marked on the basis of stable oxygen isotope <18>O, which is characterized in that: based on the fact that the N end peptide section and the C end peptide section are generally labeled, and by means of combining the <18>O chemical labeling and enzyme digestion, the quantifying strategy for two <18>O markings of the same sample can be accomplished. The method relates to the reductive alkylation and enzyme digestion of protein, dual-functional reagent modifying and <18>O marking of peptides, multi-dimensional liquid phase chromatogram separation and multistage Tandem Mass Spectrometry quantification and identification. A first mass spectrum peak area is used to quantify; and the identification of the protein is conducted through a second stage mass spectrum and by searching the database. The invention also provides a reagent box for facilitating the implementation of the method.

Methods and apparatus for characterizing a polymer sample and in preferred embodiments, libraries of polymer samples, in a comprehensive, directly-coupled multi-dimensional liquid chromatography system are disclosed. The first and second dimensions are preferably high-performance liquid chromatography dimensions, such as for example, a first dimension adapted for determining composition (e.g. adapted for mobile-phase gradient elution chromatography, including reverse phase chromatography, adsorption chromatography and the like), and a second dimension adapted for determining molecular weight or particle size (e.g., adapted for size exclusion chromatography, including gel permeation chromatography).

The invention provides a full online detection multi-dimensional liquid chromatogram separation system based on a same detector. The full online detection multi-dimensional liquid chromatogram separation system comprises a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a high performance liquid diluent pump, a gradient mixer A, a gradient mixer B, an injection valve, an enriching column array A, an enriching column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position ten-wayvalve and a connecting pipeline; the conversion of the previous-dimension separation state and the current-dimension separation state is realized through the switching of the two-position ten-way valve, so that three dimensions or more than three dimensions of chromatographic separation is realized. Each dimension of chromatographic separation column is selected through the liquid chromatographyseparation column array, full online monitoring and control for multi-dimensional chromatographic separation are realized based on the same gradient elute system and the same detector, and cleanlinessdegree controllability for enriching columns and separation columns is realized. Each dimension of separation is connected through the enriching columns, and the enrichment or gathering of compoundsis assisted by using the diluent pump. According to the system, efficient separation for complex system samples with high separation difficulty is realized by selecting the combinations of different chromatographic stationary phases and moving phases.

The invention provides improved methods and apparatus for multidimensional analysis, especially for multidimensional liquid chromatography. Methods and apparatus for the quantitative determination of one or more components comprised in a sample mixture are described. Methods and apparatus of the invention typically allow accurate quantitative determination of more components of the mixture than prior multidimensional methods. The invention is particularly useful for, but is not limited to the analysis of complex mixtures of peptides and proteins.

Methods and apparatus for characterizing a polymer sample and in preferred embodiments, libraries of polymer samples, in a comprehensive, directly-coupled multi-dimensional liquid chromatography system are disclosed. The first and second dimensions are preferably high-performance liquid chromatography dimensions, such as for example, a first dimension adapted for determining composition (e.g. adapted for mobile-phase gradient elution chromatography, including reverse phase chromatography, adsorption chromatography and the like), and a second dimension adapted for determining molecular weight or particle size (e.g., adapted for size exclusion chromatography, including gel permeation chromatography).

The invention provides a multi-dimensional liquid chromatography separation system, comprising a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a high performance liquid dilution pump, a gradient mixer A, a gradient mixer B, a sampling valve, an enriching column array A, an enriching column array B, a fraction collector, a liquid chromatographic separation column array, a detector, a two-position ten-way valve and a connecting pipeline, wherein the two-position ten-way valve switches to realize the transformation between the last-dimensional separation state and the next-dimensional separation state, so as to realize three or more-dimensional chromatographic separation. Respective-dimensional chromatographic separation columns are selected through the liquid chromatography separation column array, thereby, based on the same gradient elution system and the same detector, realizing the full-line monitoring and control ofthe multi-dimensional chromatographic separation, and realizing the controllability of cleanliness degree of the enrichment column and the separation column. Respective-dimensional separations are connected by the enrichment column, and the liquid dilution pump is used to assist in compound enrichment or capture. The system achieves efficient separation of monomer compounds in complex system samples with high separation difficulty by selecting different combinations of chromatographic stationary phases and mobile phases.

A multidimensional liquid chromatography separation system has a mobile-phase storage tank, a first liquid transfer device, a second liquid transfer device, a first sample introduction device, a second sample introduction device, a separation device, a collection, storage device, at least two drainage devices and a flow path switching device. A separation method for protein separation using the multidimensional liquid chromatography separation system has the steps of: 1) preparation in advance, 2) the first dimensional separation, 3) collection and storage of the intermediate fraction, 4) the second dimensional or multidimensional separation and repeating the steps 3) and 4).

The invention provides a multidimensional liquid chromatography-mass spectrometry method for identifying proteins and proteomes in tobacco, and the multidimensional liquid chromatography-mass spectrometry method specifically comprises the following steps: 1) taking a tobacco leaf sample, using a trichloroacetic acid-acetone precipitation method and a phenol method respectively to extract proteins,and then merging the proteins and re-dissolving to obtain a protein sample solution; 2) subjecting the protein sample solution to enzymolysis, desalting and drying to obtain dry peptide powder as a sample; and 3) sequentially passing the prepared sample through a first-dimensional liquid chromatography and a second-dimensional liquid chromatography for gathering, performing elution separation, and detecting by mass spectrometry. The method provided by the invention reserves the proteins in the sample to the maximum extent, greatly improves the peak capacity of the chromatogram, and has the advantages of high detection flux, high sensitivity and large amount of identified proteomes.

The invention discloses a polypeptide mixture isoelectric focusing separation method for proteomics analysis. The method comprises the following steps: performing restriction enzyme digestion on a total protein mixture of a biological sample completely to obtain relatively short peptide fragment mixtures; re-dissolving the peptide fragment mixtures by using a peptide fragment isoelectric focusing buffer solution; performing isoelectric focusing electrophoresis in a loading manner by adopting a loading cup, so as to realize the effective separation of a complex peptide fragment mixture; performing desalination on a taken polypeptide mixture pre-separated from each groove and performing mass spectrometry analysis so as to obtain more polypeptide signals, higher peptide fragment covering rate and higher protein identification number compared with those obtained when a conventional method is adopted. The method is precise, efficient and low in cost, can be used in multidimensional liquid chromatography-mass spectrum identification technology systems of total proteins of the various biological samples, can replace a conventional ion exchange chromatography pre-separation method so as to realize the efficient pre-separation of the peptide fragment mixtures, and avoids the lost of peptide fragments, and therefore, proteome expression profile information with the high covering rate can be established.

The invention provides a multi-dimensional liquid chromatography separation system. The system comprises a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a high performance liquid dilution pump, a gradient mixer A, a gradient mixer B, a sample feeding valve, an enrichment column array, a fraction collector, a liquid chromatographyseparation column array, a detector, a two-position six-way valve and a connecting pipeline. A chromatography separation column of each dimension is selected through the liquid chromatography separation column array; multi-dimensional chromatography separation is monitored and controlled fully online through the same gradient eluting system and the same detector, so that cleaning level of the enrichment column and the separation column can be controlled. Separation of all dimensions are connected through enrichment columns, and the dilution pump is employed to aid enrichment and capture of compounds. Monomeric compounds hard to separate in a complex system sample can be separated efficiently by selecting different combinations of chromatographic stationary phases and mobile phases.

The invention provides a multi-dimensional liquid chromatography separation system. The system comprises a high-performance liquid chromatography gradient pump A, a high-efficiency liquid chromatography gradient pump B, a high-performance liquid chromatography diluting pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a double-two-position four-way valve and a connecting pipeline; and switching between a previous one-dimensional separation state and a next one-dimensional separation state is realized through switching of the double-two-position four-way valve, and chromatography separation of three dimensions or above is realized. A chromatography separation column of each dimension is selected through the liquid chromatography separation column array; and on the basis of the same gradient elution system and the same detector, full online monitoring and control of multi-dimensional chromatography separation are realized, and the cleaning degree of enrichment columns and separation columns is controllable. The separationof all dimensions is connected through the enrichment columns, and the diluting pump is used for assisting in enrichment or trapping of compounds. Different chromatography stationary phase and mobilephase combinations are selected, so that efficient separation of monomeric compounds in a complex system sample with high separation difficulty is realized.

The invention relates to a liquid-phase chromatographic column, in particular to an array multi-dimensional liquid-phase chromatographic column system. The system is provided with a high-pressure nanofluidic pump, a single chromatographic column, a multi-channel chromatography connection device and parallel array chromatographic columns. The high-pressure nanofluidic pump, the single chromatographic column, the multi-channel chromatography connection device and the parallel array chromatographic columns are sequentially connected in series, outlets of the parallel array chromatographic columns are connected with an inlet of a multi-channel ultraviolet detector, and an outlet of the multi-channel ultraviolet detector is connected with at least two empty capillary tubes with detection windows. First-dimension chromatographic separation is achieved through ion exchange chromatography, and after first salt gradient separation is completed, at the tails of the columns, eluate is equally divided and gathered to multiple reversed phase chromatographic column heads with the same separation effect; then, the multiple elution components are separated at the same time in a multi-column parallel mode at the second dimension. The operation is repeated until all salt gradients are run, and then all two-dimensional chromatographic separation processes are completed. Through the array two-dimensional chromatography system, a high-resolution two-dimensional separation effect can be achieved, and multiple sample flows can be obtained through one-off separation running.

The invention relates to the field of medical detection and in particular to a blood aripiprazole therapeutic drug monitoring kit developed based on multi-dimensional online solid-phase extraction liquid chromatography analytical technology, more specifically to a method for accurately and quantitatively detecting aripiprazole drug concentration in blood by online solid-phase extraction and multi-dimensional liquid chromatography analytical technology. The kit includes a calibration reagent, a quality control reagent, a processing liquid, an extract liquid, a washing liquid, and an eluent. Thetherapeutic drug monitoring kit of the invention only needs to centrifuge a blood sample, achieves low repeated detection variability, high accuracy and stability, and low detection cost, has variousmethodological indicators that satisfy the requirement for monitoring the blood aripiprazole therapeutic drugs, and is easy to generalize.

The invention provides a multi-dimensional liquid chromatography separation system. The system comprises a high-performance liquid chromatography gradient pump A, a high-efficiency liquid chromatography gradient pump B, a high-performance liquid chromatography diluting pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position eight-way valve and a connecting pipeline; and conversion between a previous one-dimensional separation state and a next one-dimensional separation state is realized through switching of the two-position eight-way valve, and chromatographic separation of three dimensions or above is realized. A chromatographic separation column of each dimension is selected through the liquid chromatography separation column array; and on the basis of the same gradient elution system and the same detector, fullonline monitoring and control of multi-dimensional chromatography separation are realized, and the cleaning degree of enrichment columns and separation columns is controllable. The separation of all dimensions is connected through the enrichment columns, and the diluting pump is used for assisting in enrichment or trapping of compounds. Different chromatography stationary phase and mobile phase combinations are selected, so that efficient separation of monomeric compounds in a complex system sample with high separation difficulty is realized.

The invention provides a multi-dimensional liquid chromatogram separation system comprising a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradientpump B, a high performance liquid phase dilution liquid pump, a gradient mixer A, a gradient mixer B, a sampling valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a dual two-position four-way valve and a connecting pipeline. With switching of the dual two-position four-way valve, conversion betweena last one-dimensional separation state and a next one-dimensional separation state is realized and thus three-dimensional or over three-dimensional chromatographic separation is realized. The liquidchromatography separation column array is used for selecting all-dimensional chromatographic separation columns; and with the same gradient elution system and the same detector, all on-line monitoringand controlling of multi-dimensional chromatographic separation as well as the cleaning degree controllability of the enrichment column and the separation column are realized. All-dimensional separation is connected by the enrichment columns and compound enrichment or capturing is assisted by using the diluent pump. According to the invention, high-efficiency separation of the monomeric compoundin the complex system sample with the high separation difficulty is realized by selecting different chromatographic stationary phase and mobile phase combinations.

The invention relates to the field of medical detection and in particular to a blood donepezil therapeutic drug monitoring kit developed based on multi-dimensional online solid-phase extraction liquidchromatography analytical technology, more specifically to a method for accurately and quantitatively detecting donepezil drug concentration in blood by online solid-phase extraction and multi-dimensional liquid chromatography analytical technology. The kit includes a calibration reagent, a quality control reagent, a processing liquid, an extract liquid, a washing liquid, and an eluent. The therapeutic drug monitoring kit of the invention only needs to centrifuge a blood sample, achieves low repeated detection variability, high accuracy and stability, and low detection cost, has various methodological indicators that satisfy the requirement for monitoring the blood donepezil therapeutic drugs, and is easy to generalize.

The invention relates to the field of medical detection and in particular to a blood vancomycin therapeutic drug monitoring kit developed based on multi-dimensional online solid-phase extraction liquid chromatography analytical technology, more specifically to a method for accurately and quantitatively detecting vancomycin drug concentration in blood by online solid-phase extraction and multi-dimensional liquid chromatography analytical technology. The kit includes a calibration reagent, a quality control reagent, a processing liquid, an extract liquid, a washing liquid, and an eluent. The therapeutic drug monitoring kit of the invention only needs to centrifuge a blood sample, achieves low repeated detection variability, high accuracy and stability, and low detection cost, has various methodological indicators that satisfy the requirement for monitoring the blood vancomycin therapeutic drugs, and is easy to generalize.

The invention provides a multi-dimensional liquid chromatography separation system comprising a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a high performance liquid phase dilution liquid pump, a gradient mixer A, a gradient mixer B, a sampling valve, an enriched column array A, an enriched column array B, a distillate collector, a liquid chromatography column array, a detector, a two-position eight-way valve and a connecting pipeline. Conversion from the previous dimensional separation state to the next dimensional separation state is realized by the two-position eight-way valve to achieve three or more-dimensional chromatographic separation. Through the liquid chromatography separation column array, each-dimensional chromatographic separation column is selected, thereby, based on the same gradient elution system and the same detector, realizing the all on-line monitoring and control of multi-dimensional chromatographic separation and realizing controllable degree of cleanliness of the enrichment columns and separation columns. Each-dimensional separation is connected by an enrichment column and a diluent pump is used to assist in compound enrichment or capture. The multi-dimensional liquid chromatography separation system provided by the invention achieves efficient separation of monomer compounds in complex system samples with high separation difficulty by selecting different combinations of chromatographic stationary phases and mobile phases.

This invention provides to rare earth element metal chelation label protein for quantitative protein study method and agent case. This invention method relates to protein restore alkali base and pancreatic enzymes slices, peptide section double function agent decoration, rare metal chelation, multi-diemension liquid phase spectrum isolation and multiple degrees series spectrum quantitative and testing. Through one degree spectrum ion signal intensity content and protein validating through series mass spectrum and database. This invention also describes this method agent case.

The invention relates to a three-dimensional liquid chromatography separation system, comprising a high-performance liquid chromatography pump A, a high-performance liquid chromatography pump B, a gradient mixer A, a gradient mixer B, an injection valve, a two-position ten-way valve, a two-position six-way valve, an enrichment column array A, an enrichment column array B, a liquid chromatography separation column array, a detector, a diluent pump, a fraction collector and a connecting pipeline. According to the scheme of the invention, the two-position ten-way valve and the two-position six-way valve are used for switching multi-dimensional separation states, so that one-dimensional, two-dimensional or three-dimensional chromatography separation capabilities are realized. The three-dimensional liquid chromatography separation system has strong expansibility, and can be upgraded to a multi-dimensional liquid chromatography separation system only by reconnecting system pipelines on the premise of not increasing the system cost, so that the system has higher dimensional separation capabilities such as four-dimensional chromatography separation and the like; the system can also be taken as a common full three-dimensional liquid chromatography separation system by reconnecting the pipelines when necessary; and the system has a very broad application prospect in the separation and analysis of complex system samples with high separation difficulty.

The invention relates to the field of medical detection and in particular to a blood amiodarone therapeutic drug monitoring kit developed based on multi-dimensional online solid-phase extraction liquid chromatography analytical technology, more specifically to a method for accurately and quantitatively detecting amiodarone drug concentration in blood by online solid-phase extraction and multi-dimensional liquid chromatography analytical technology. The kit includes a calibration reagent, a quality control reagent, a processing liquid, an extract liquid, a washing liquid, and an eluent. The therapeutic drug monitoring kit of the invention only needs to centrifuge a blood sample, achieves low repeated detection variability, high accuracy and stability, and low detection cost, has various methodological indicators that satisfy the requirement for monitoring the blood amiodarone therapeutic drugs, and is easy to generalize.