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Chicken heat resistance-associated molecular marker and identification method and use thereof

A molecular marker and identification method technology, applied in the field of genetic engineering and genetic breeding, to achieve the effect of simple conditions, high reliability and low price

Inactive Publication Date: 2011-08-17
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0004] At present, there are few studies on molecular markers for chicken heat resistance, and the heat resistance molecular markers found in the 5' flanking region of chicken Hsp90-b in the present invention are discovered for the first time

Method used

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  • Chicken heat resistance-associated molecular marker and identification method and use thereof
  • Chicken heat resistance-associated molecular marker and identification method and use thereof
  • Chicken heat resistance-associated molecular marker and identification method and use thereof

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Embodiment

[0033] When the indoor ambient temperature was 35°C and 15°C, 333 Bantamina blood were collected from the subwing vein and divided into three tubes: non-anticoagulant blood 1-2mL, serum for T3 and corticosterone determination; anti-coagulant blood 1-2mL 2mL, used to determine H / L, and CD 3+ 、CD 4+ . 1-2ml of anticoagulated blood is used for the extraction of genomic DNA. Among them, non-anticoagulated blood was directly centrifuged to absorb serum, and T3 and corticosterone were measured by enzyme-linked immunosorbent assay; anticoagulated blood was used to measure H / L, and the blood smear was stained by Wright’s and Giemsa’s composite staining method, and microscopic examination was performed. , carry out cell classification according to Alfred's leukocyte classification method, and count and calculate according to Campo's method; CD 3+ 、CD 4+ Detection by flow cytometry.

[0034] Extract the genomic DNA of 666 blood samples, take 10ul for each sample, and make DNA dilut...

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Abstract

The invention discloses a chicken heat resistance-associated molecular marker and an identification method and use thereof. The molecular marker is G-141A which is positioned in a 5' flanking region in a chicken heat stress protein 90-beta gene. In the invention, the polymerase chain reaction (PCR) amplification of the 5' flanking region in the chicken heat stress protein 90-beta gene, the product of the amplification is subjected to sequencing and single nucleotide polymorphism (SNP) screening, the screened SNP is subjected to PCR-restriction fragment length polymorphism(RFLP) in a large population, the obtained typing result and the heat resistance property index of the population are subjected to association analysis, an SNP locus which is associated obviously is initially determined as heat resistance property auxiliary selection molecular marker, and chicken of a GG genotype has high heat resistance in a high-temperature environment.

Description

technical field [0001] The invention relates to the fields of genetic engineering and genetic breeding, in particular to a molecular marker related to chicken heat resistance, an identification method and application thereof. Background technique [0002] Molecular marker-assisted selection is an important application of genetic engineering in modern poultry breeding. The method of molecular markers can not only shorten the breeding time limit, but also greatly reduce the human and material resource consumption of breeding. In addition, the diversity of molecular markers makes molecular markers more popular in animals. The application potential in breeding is greatly improved. High temperature seasons are likely to cause heat stress in chickens, resulting in slow growth and development, decreased immunity, increased morbidity and mortality, and brought huge economic losses to intensive chicken production. At present, comprehensive measures such as ventilation and cooling an...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 罗庆斌甘建伉张细权陈卓宇聂庆华凌胜
Owner SOUTH CHINA AGRI UNIV
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