The invention relates to a factory-like large-scale seed culture device and method for marsupenaeus japonicus, and belongs to the technical field of prawn farming. The method includes the following processing steps that 1, a culture pond is constructed; 2, sanding is conducted; 3, a gas-lift unit is installed; 4, disinfecting is carried out; 5, inflow is performed; 6, seeds are put in; 7, the water color is adjusted; 8, bait is thrown; 9, the water quality and growth conditions are monitored; 10, the seeds are moved out of the pond. According to the method, factory-like high-density and large-scale (about 3 cm) seed culture can be conducted, the culture period in the culture pond is shortened, and the large-scale seed culture device and method for the marsupenaeus japonicus which is cultured for multiple batches in one year are realized. By the adoption of the method, the body length of the seeds is cultured to be about 3 cm from 0.8 cm, and the survival rate of the marsupenaeus japonicus can reach 80%; the marsupenaeus japonicus is cultured into commercial sized marsupenaeus japonicus from the body length of about 3 cm, the survival rate of the marsupenaeus japonicus can reach 70%, and the yield can reach 1500-2250 kg / hm2.