45 results about "Marsupenaeus" patented technology

A method for building pedigree of marsupenaeus japonicus and breeding relates to the breeding of the penacus orientalis. The method comprises the following steps: building the base population; building the F1 generation pedigree; cultivating in a same pond; building the F2 generation close cousins pedigree; after growing to 3 cm, cultivating in the same pond, and selecting the breeding parents while reaching the specification of goods to generally build 8-16 close cousins pedigrees, wherein each close cousins pedigree retains 5-8 fullsibs family, the total number of the breeding pedigrees is 60-80, and the breeding parents in each pedigree are continuously bred to mature parents; and adopting a continuous multi-generation hybrid system to continuously build the close cousins pedigree of the next generation until to reach the breeding target and form the new fast growing variety. The invention is a method for building close cousins pedigree of marsupenaeus japonicus and breeding, especially can be applied to the inheritance breeding of the marsupenaeus japonicus, and has important significance for the breeding design of the marine animals which are not suitable for the fine breeding mode.

The invention provides an SNP (single-nucleotide polymorphism) marker related to Marsupenaeus japonicus heat resistance and a detection method thereof, relating to detection of shrimp heat-resistant populations in aquiculture. The invention firstly provides an SNP marker related to Marsupenaeus japonicus heat tolerance by using a direct sequencing process. The SNP marker related to Marsupenaeus japonicus heat tolerance is the 3289th site of the HSP60 gene of which the nucleic acid sequence is SEQ ID NO.1, and the base is A or G. The detection method comprises the following steps: carrying out a Marsupenaeus japonicus heat resistance experiment; calculating the heat resistance value; carrying out genotyping by using a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technique; and analyzing the heat tolerance of the Marsupenaeus japonicus individuals with different genotypes. The direct sequencing process and the PCR-RFLP process are utilized to give out the polymorphism of the MjHSP60 gene, and the correlation analysis is carried out on the SNP polymorphism and Marsupenaeus japonicus heat tolerance, thereby providing theoretical references for screening high-temperature-resistant SNP molecular markers and developing molecular breeding of Marsupenaeus japonicus heat-resistant lines.

The invention discloses a method for controlling a white spot syndrome through mixed culture of Lates calcarifer and Marsupenaeus japonicas. According to the method, 70,000-80,000 young Marsupenaeus japonicas prawns with the overall length being 0.8-1.0 cm are put in each mu of a pond, 8-10 cm long Lates calcarifer are put when the Marsupenaeus japonicas prawns grow to 4-5 cm through culture, and 5-6 Lates calcarifer are put in each mu of the pond. According to the culture method, Lates calcarifer in an appropriate density and in appropriate specifications are put in a high-density intensive culture pond of Marsupenaeus japonicas according to the living habits of Lates calcarifer and Marsupenaeus japonicas, illness and death of the prawns in the culture process can be reduced, a good ecological breeding environment is provided for growth of the Marsupenaeus japonicas, the survival rate of the prawns is increased, the morbidity is reduced, the white spot syndrome is effectively controlled, the culture success rate is high, and the method is suitable for large scale farming development of Marsupenaeus japonicas in China.

The invention discloses a portable transportation device and a transportation method for marsupenaeus japonicus sea-caught seed shrimps. The device is characterized by comprising a constant-temperature box body, the interior of the constant-temperature box body is divided into three cavities, namely, a shrimp storage chamber, an ice storage chamber and an equipment chamber, shrimp grids for storing seed shrimps are arranged in the shrimp storage chamber; a refrigerant is placed in the ice storage chamber; a controller is arranged in equipment room, a water filtering system for circularly filtering water in the shrimp storage chamber and an oxygen supply system for supplying oxygen to the shrimp storage chamber are arranged between the equipment chamber and the shrimp storage chamber; the ice storage chamber is connected with an oxygen supply system through a pipeline; the temperature control system is electrically connected with the controller; the temperature control system is used for detecting the water temperature in the shrimp storage chamber and controlling the pipelines to be connected or disconnected according to the water temperature. The device has the advantages that theconstant temperature can be kept all the time, the operation frequency during seed shrimp transportation is reduced, the emergency response of seed shrimps during high temperature is reduced, the survival rate of the seed shrimps is increased, and the life vitality of the seed shrimps is guaranteed.

The invention relates to a PCR amplification primer for a chondriosome cytb gene segment of Marsupenaeus japonicus and an identification method thereof, belonging to the field of shrimps population identification. The invention provides a pair of PCR amplification primers for identifying the geographic group of the Marsupenaeus japonicus, respectively containing the nucleotide sequences of L10945:5'-TGAGGAGGTTTCGCAGTA-3' or H11563: 5'-AGATGAGGGTGAGTGGGT-3'. The identification method comprises the steps: firstly, carrying out PCR amplification on a cytochrome b gene segment in the chondriosome DNA of a sample of four geographic groups to be detected by using the pair of amplification primers; and secondly, purifying the amplification primer and sequencing, wherein a sample to be detected with the 528-locus and the 534-locus bases of the nucleotide sequences to G is from Fujian group. The invention has the characteristics of stable amplification result, favorable repeatability and simple and effective identification method.

The invention relates to a factory-like large-scale seed culture device and method for marsupenaeus japonicus, and belongs to the technical field of prawn farming. The method includes the following processing steps that 1, a culture pond is constructed; 2, sanding is conducted; 3, a gas-lift unit is installed; 4, disinfecting is carried out; 5, inflow is performed; 6, seeds are put in; 7, the water color is adjusted; 8, bait is thrown; 9, the water quality and growth conditions are monitored; 10, the seeds are moved out of the pond. According to the method, factory-like high-density and large-scale (about 3 cm) seed culture can be conducted, the culture period in the culture pond is shortened, and the large-scale seed culture device and method for the marsupenaeus japonicus which is cultured for multiple batches in one year are realized. By the adoption of the method, the body length of the seeds is cultured to be about 3 cm from 0.8 cm, and the survival rate of the marsupenaeus japonicus can reach 80%; the marsupenaeus japonicus is cultured into commercial sized marsupenaeus japonicus from the body length of about 3 cm, the survival rate of the marsupenaeus japonicus can reach 70%, and the yield can reach 1500-2250 kg / hm2.

The invention provides a method for replanting waste spermatophore of marsupenaeus japonicus, relating to a method for artificial propagation of marine shrimps. The method for replanting waste spermatophore of marsupenaeus japonicus, which is provided by the invention, comprises the following steps: stripping the original complete spermatophore from the seminal receptacle of the female broodstockof the marsupenaeus japonicus which just dies and molts, and cleaning the spermatophore for later use; and taking the female broodstock of the marsupenaeus japonicus the carapace of which is not hardened, sticking a bonding agent at the bottom of the spermatophore, filling the spermatophore into the seminal receptacle of the female broodstock, and then, sterilizing the female broodstock for culturing. The method provided by the invention has simple and understandable principles and is simple and convenient in operation, and the maturing production process of the female broodstock can be carried out at any time according to the needs; and the used bonding agent has wide material sources and is economical and practical. The invention fills the blank of artificial mating of the molted femalebroodstock of the marsupenaeus japonicus in natural sea areas, and has the advantages of very low risk factor in application and obvious economical benefit.

The invention discloses a filter bed breeding system and a factory breeding method of buried-dwelling marine organisms, and belongs to the field of aquiculture. The breeding system comprises a breeding pond, an empty space is arranged at the bottom of the breeding pond, a sand layer filter bed is arranged on the empty space, and a breeding water layer is above the sand layer filter bed; the breeding pond is internally provided with an air-life tube, one end of the air-lift tube communicates with the bottom of the breeding pond, and the other end of the air-lift tube communicates with the breeding water layer; a first oxygen increasing tube is arranged at the bottom of the breeding pond; and a second oxygen increasing tube is arranged at the bottom of the breeding water layer. S and divinghabits of the buried-dwelling marine organisms such as Marsupenaeus japonicus and Babylonia can be satisfied, the problem of bottom substrate changing black during high-density breeding is advantageously solved, the adhesion and purification effect of probiotics are improved, breeding stability is improved, the filter bed breeding system and the factory breeding method of the buried-dwelling marine organisms are suitable for high-density factory breeding of the buried-dwelling marine organisms, and has a good application prospect.

Provided is a grading and optimizing method for ammonia nitrogen resistance of marsupenaeus japonicas shrimp seeds. P=(-374237.401C2+60262.529C4-482.009C6-5837.357C10+2728.598C13+161295.154C14+6521.543C15+575.301C16+7363.539C17-15438.198) / (-373239.473C2+59971.405C4-204.015C6-5345.370C10+2773.543C13+160584.988C14+6394.044C15+566.017C16+7468.213C17-15349.352). The method determines and screens out shrimp seeds in good ammonia nitrogen resistance according to the value of P.

The invention provides a method for accurately and quickly identifying two morphological variants of marsupenaeus japonicus, which relates to the marsupenaeus japonicus. The method comprises the following steps of: designing and amplifying primers of cytochrome b gene segments of the marsupenaeus japonicus; and extracting the DNA of the variants I and II of the marsupenaeus japonicus, performing polymerase chain reaction (PCR) by taking the DNA as a template, performing enzyme cutting on PCR products and performing electrophoresis identification on an enzyme-cutting products, wherein the variant I of the marsupenaeus japonicus has the enzyme cutting sites of gatatc and produces the two enzyme-cutting segments of 284bp and 299bp by the enzyme cutting; and the variant II of the marsupenaeusjaponicus does not have any enzyme-cutting site and still has the strip of 583bp, so that the two variants of the marsupenaeus japonicus can be identified.

The invention discloses a portable transport device and a transport method for prawn prawns caught in the sea, characterized in that the device includes a constant temperature box, and the interior of the constant temperature box is divided into three chambers: a shrimp storage room, an ice storage room and equipment In the shrimp storage room, there are shrimp grids for storing shrimp, in the ice storage room, there is a refrigerant, in the equipment room, there is a controller, and between the equipment room and the shrimp storage room, there is a device for circulating and filtering the water in the shrimp storage room. The water filtration system and the oxygen supply system used to supply oxygen to the shrimp storage room, the ice storage room is connected to the oxygen supply system through pipelines, and also includes a temperature control system electrically connected to the controller. The temperature control system uses It is used to detect the water temperature in the shrimp storage room and control the connection or closure of the pipeline according to the water temperature. The advantages are: it can always maintain a constant temperature, reduce the number of operations during the transportation of the shrimp, thereby reducing the emergency response of the shrimp during high temperature, and improving the survival rate of the shrimp. Ensure the vitality of the shrimp.

The invention discloses a multi-crop polyculture method in ponds for increasing the yield of Penaeus japonicus, and belongs to the technical field of aquaculture. The above method includes: step 1: pond selection and treatment; step 2: shrimp seed selection and release; step 3: crab seed selection; step 4: daily water quality management; step 5: feeding management; step 6: timely fishing. In summary, this application discloses the multi-crop polyculture model of Penaeus trituberculatus and Portunus trituberculatus. During the polyculture of Penaeus trituberculatus and Portunus trituberculatus, Portunus trituberculatus will eat the carcasses of Penaeus trituberculatus, which can effectively prevent shrimp diseases further dissemination; the breeding steps are scientific and reasonable, which can effectively increase the yield and survival rate of Penaeus japonicus, and the two together increase economic benefits.