45 results about "Marsupenaeus" patented technology

A method for building pedigree of marsupenaeus japonicus and breeding relates to the breeding of the penacus orientalis. The method comprises the following steps: building the base population; building the F1 generation pedigree; cultivating in a same pond; building the F2 generation close cousins pedigree; after growing to 3 cm, cultivating in the same pond, and selecting the breeding parents while reaching the specification of goods to generally build 8-16 close cousins pedigrees, wherein each close cousins pedigree retains 5-8 fullsibs family, the total number of the breeding pedigrees is 60-80, and the breeding parents in each pedigree are continuously bred to mature parents; and adopting a continuous multi-generation hybrid system to continuously build the close cousins pedigree of the next generation until to reach the breeding target and form the new fast growing variety. The invention is a method for building close cousins pedigree of marsupenaeus japonicus and breeding, especially can be applied to the inheritance breeding of the marsupenaeus japonicus, and has important significance for the breeding design of the marine animals which are not suitable for the fine breeding mode.

The invention relates to the technical field of food processing and storing, and discloses a fresh keeping method of marsupenaeus japonicus through deepfreezing of liquid nitrogen. Working procedures of performing cleaning, performing draining, preventing frostbite, performing multi-stage quick-freezing, plating ice coating, performing packaging and the like are sequentially performed, so that a set of complete fresh keeping technology for the marsupenaeus japonicus through the deepfreezing of the liquid nitrogen is formed. Ice crystals formed by products after deepfreezing treatment are small, so that the integrity of product cells is guaranteed, the original color, the original freshness and the original flavor of the products can also be maintained, the shelf life of the products is greatly prolonged, the fresh keeping degree is good, supply requirements throughout the year can be met, and the fresh keeping method has good application value.

The invention provides a breeding method for marsupenaeus japonicus breeding population based on high ammonia nitrogen resistance and growth traits. The method comprises the steps of: (1) parent shrimp import; (2) establishment of a family; (3) preliminary experiment of high ammonia nitrogen resistance; (4) high ammonia nitrogen resistance experiment for the breeding family; (5) high ammonia nitrogen resistance data recording; (6) establishment of a genetic estimation model; (7) family breeding larva reservation; (8) establishment of a second-generation family. The method can breed a new marsupenaeus japonicus variety with high ammonia nitrogen resistance and growth superiority; the stress resistance of marsupenaeus japonicus to the concentration of ammonia nitrogen is improved and the survival rate and the growth speed of marsupenaeus japonicus are increased.

The invention relates to a factory-like large-scale seed culture device and method for marsupenaeus japonicus, and belongs to the technical field of prawn farming. The method includes the following processing steps that 1, a culture pond is constructed; 2, sanding is conducted; 3, a gas-lift unit is installed; 4, disinfecting is carried out; 5, inflow is performed; 6, seeds are put in; 7, the water color is adjusted; 8, bait is thrown; 9, the water quality and growth conditions are monitored; 10, the seeds are moved out of the pond. According to the method, factory-like high-density and large-scale (about 3 cm) seed culture can be conducted, the culture period in the culture pond is shortened, and the large-scale seed culture device and method for the marsupenaeus japonicus which is cultured for multiple batches in one year are realized. By the adoption of the method, the body length of the seeds is cultured to be about 3 cm from 0.8 cm, and the survival rate of the marsupenaeus japonicus can reach 80%; the marsupenaeus japonicus is cultured into commercial sized marsupenaeus japonicus from the body length of about 3 cm, the survival rate of the marsupenaeus japonicus can reach 70%, and the yield can reach 1500-2250 kg / hm2.

The invention relates to an optimum selection method of a wild parent of an autumn shrimp of Marsupenaeus japonicus and a fundamental group morphological model obtaining method. Marsupenaeus japonicus summer wild individual shrimps having the body types meeting a morphological model equation of a Marsupenaeus japonicus autumn shrimp high-quality wild parent selection fundamental group are selected into an autumn shrimp high-quality wild parent fundamental group; after artificial ripening promotion and culture, female shrimps that have characteristics of bright and clean body surfaces, bright and transparent shells, bright body colors, complete appendages, healthy states with no disease and no damage, quick reaction, good gonad development, and long body lengths larger than or equal to 16cm are selected as Marsupenaeus japonicus autumn shrimp breeding parents. Therefore, the overwintering breeding survival rate of the Marsupenaeus japonicus autumn shrimps is effectively improved.

The invention discloses a combined method of a stereo ecological polyculture mode and the prevention and control of hepatopancreatic necrosis disease and white spot syndrome. Specifically, 3 days before adding prawn seeds, 200 scatophagus argus fries with length of 3-5cm and 300 siganids fries with length of 2-3cm are added into every Mu of a breeding pond; when prawn seeds are to be added, a prawn seed addition amount is 80000-100000 seeds / Mu; when prawn length reaches 4-5cm, 5-6 Nile perch with length of 8-10cm and 10-15 hybrid giant tiger groupers with length of 8-10cm are added into every Mu of the pond. According to the invention, fishes with different feeding habits are cultured with prawns at a same time, such that diseases and weak prawns are eliminated in time, prawn disease infection source is eliminated, and a water cleaning function is realized. A polyculture mode of prawns and fishes with suitable specifications is started from a breeding early-mid stage, such that a prawn disease infection source is cut off, and hepatopancreatic necrosis disease and white spot syndrome outbreak is prevented. The breeding mode is optimized, such that prawn breeding success rate is improved. The method provided by the invention is suitable for the breeding of prawns such as litopenaeus vannamei, marsupenaeus japonicas, penaeus monodon and the like.

The invention discloses a method for controlling a white spot syndrome by culturing hybrid giant tiger groupers and marsupenaeus japonicas in a mixed mode. The method comprises the steps that marsupenaeus japonicas seeds with the overall length of 0.8-1.0 cm are put into a pond, the putting quantity is 70,000-80,000 per mu, the hybrid giant tiger groupers with the body length of 8-10 cm are put into the pond when the marsupenaeus japonicas seeds are cultured until the body length of each marsupenaeus japonicas seed is 4-5 cm, and the putting quantity is 10-15 per mu. According to the method, according to the life habits of the hybrid giant tiger groupers and the marsupenaeus japonicas, by putting the hybrid giant tiger groupers in the appropriate density and appropriate specification into the high-density intensive culturing pond of the marsupenaeus japonicas, occurrence of the diseased and dead marsupenaeus japonicas can be reduced in the marsupenaeus japonicas culturing process, a good ecological culturing environment is supplied to growth of the marsupenaeus japonicas, the survival rate of the marsupenaeus japonicas is increased, the incidence is decreased, the culturing success rate is high, and the method is suitable for development of scale culturing of the marsupenaeus japonicas in China.

The invention provides an SNP (single-nucleotide polymorphism) marker related to Marsupenaeus japonicus heat resistance and a detection method thereof, relating to detection of shrimp heat-resistant populations in aquiculture. The invention firstly provides an SNP marker related to Marsupenaeus japonicus heat tolerance by using a direct sequencing process. The SNP marker related to Marsupenaeus japonicus heat tolerance is the 3289th site of the HSP60 gene of which the nucleic acid sequence is SEQ ID NO.1, and the base is A or G. The detection method comprises the following steps: carrying out a Marsupenaeus japonicus heat resistance experiment; calculating the heat resistance value; carrying out genotyping by using a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technique; and analyzing the heat tolerance of the Marsupenaeus japonicus individuals with different genotypes. The direct sequencing process and the PCR-RFLP process are utilized to give out the polymorphism of the MjHSP60 gene, and the correlation analysis is carried out on the SNP polymorphism and Marsupenaeus japonicus heat tolerance, thereby providing theoretical references for screening high-temperature-resistant SNP molecular markers and developing molecular breeding of Marsupenaeus japonicus heat-resistant lines.

Provided is a grading and optimizing method for ammonia nitrogen resistance of marsupenaeus japonicas shrimp seeds. P=(-374237.401C2+60262.529C4-482.009C6-5837.357C10+2728.598C13+161295.154C14+6521.543C15+575.301C16+7363.539C17-15438.198) / (-373239.473C2+59971.405C4-204.015C6-5345.370C10+2773.543C13+160584.988C14+6394.044C15+566.017C16+7468.213C17-15349.352). The method determines and screens out shrimp seeds in good ammonia nitrogen resistance according to the value of P.

The invention discloses a method for controlling a white spot syndrome through mixed culture of Lates calcarifer and Marsupenaeus japonicas. According to the method, 70,000-80,000 young Marsupenaeus japonicas prawns with the overall length being 0.8-1.0 cm are put in each mu of a pond, 8-10 cm long Lates calcarifer are put when the Marsupenaeus japonicas prawns grow to 4-5 cm through culture, and 5-6 Lates calcarifer are put in each mu of the pond. According to the culture method, Lates calcarifer in an appropriate density and in appropriate specifications are put in a high-density intensive culture pond of Marsupenaeus japonicas according to the living habits of Lates calcarifer and Marsupenaeus japonicas, illness and death of the prawns in the culture process can be reduced, a good ecological breeding environment is provided for growth of the Marsupenaeus japonicas, the survival rate of the prawns is increased, the morbidity is reduced, the white spot syndrome is effectively controlled, the culture success rate is high, and the method is suitable for large scale farming development of Marsupenaeus japonicas in China.

The invention provides a method for accurately and quickly identifying two morphological variants of marsupenaeus japonicus, which relates to the marsupenaeus japonicus. The method comprises the following steps of: designing and amplifying primers of cytochrome b gene segments of the marsupenaeus japonicus; and extracting the DNA of the variants I and II of the marsupenaeus japonicus, performing polymerase chain reaction (PCR) by taking the DNA as a template, performing enzyme cutting on PCR products and performing electrophoresis identification on an enzyme-cutting products, wherein the variant I of the marsupenaeus japonicus has the enzyme cutting sites of gatatc and produces the two enzyme-cutting segments of 284bp and 299bp by the enzyme cutting; and the variant II of the marsupenaeus japonicus does not have any enzyme-cutting site and still has the strip of 583bp, so that the two variants of the marsupenaeus japonicus can be identified.

The invention provides an immune health care marsupenaeus japonicus compound feed. The feed comprises a base material and an auxiliary material, and the base material comprises imported Japanese fishmeal, fully defatted fish meal, semi-skimmed fish meal, fermented soybean meal, 46% soybean meal, flour, Antarctic phosphorus shrimp powder, wall breaking yeast powder, a squid cream, an enzymatic hydrolyzed shrimp paste, soybean phospholipid oil and refined fish oil; the auxiliary material includes dicalcium phosphate, bentonite, choline chloride, a composite multivitamin, a composite mineral, atrace element premix material, a microecological preparation, organic acid, a mildew inhibitor and haematococcus pluvialis.

The invention relates to an evaluation method for the releasing effect of Marsupenaeus Japonicus. The method includes: conducting amplification and sequencing on maternally inherited mitochondrial sequences, analyzing a genetic distance by relevant software, and carrying out system construction, haplotype analysis and the like to analyze sequence differences and haplotype distribution of wild populations and breeding populations, counting the intra-population frequency of each haplotype, selecting one or several specific parent shrimps with specific molecular markers to breed first filial generation of small shrimps for releasing, thus determining the proportion of individuals in recaptured populations, and performing comparison with the proportion of the molecular markers before releasing so as to determine the releasing effect of the batch. The method provided by the invention has the characteristics of simple process and low cost. Based on the method, scientific releasing planning can be made to effectively promote recovery of wild Marsupenaeus Japonicus population resources, and filial generations of released individuals can also be detected. The method provided by the invention also can be used for years of continuous monitoring.

The invention discloses a portable transportation device and a transportation method for marsupenaeus japonicus sea-caught seed shrimps. The device is characterized by comprising a constant-temperature box body, the interior of the constant-temperature box body is divided into three cavities, namely, a shrimp storage chamber, an ice storage chamber and an equipment chamber, shrimp grids for storing seed shrimps are arranged in the shrimp storage chamber; a refrigerant is placed in the ice storage chamber; a controller is arranged in equipment room, a water filtering system for circularly filtering water in the shrimp storage chamber and an oxygen supply system for supplying oxygen to the shrimp storage chamber are arranged between the equipment chamber and the shrimp storage chamber; the ice storage chamber is connected with an oxygen supply system through a pipeline; the temperature control system is electrically connected with the controller; the temperature control system is used for detecting the water temperature in the shrimp storage chamber and controlling the pipelines to be connected or disconnected according to the water temperature. The device has the advantages that theconstant temperature can be kept all the time, the operation frequency during seed shrimp transportation is reduced, the emergency response of seed shrimps during high temperature is reduced, the survival rate of the seed shrimps is increased, and the life vitality of the seed shrimps is guaranteed.

The invention relates to a PCR amplification primer for a chondriosome cytb gene segment of Marsupenaeus japonicus and an identification method thereof, belonging to the field of shrimps population identification. The invention provides a pair of PCR amplification primers for identifying the geographic group of the Marsupenaeus japonicus, respectively containing the nucleotide sequences of L10945:5'-TGAGGAGGTTTCGCAGTA-3' or H11563: 5'-AGATGAGGGTGAGTGGGT-3'. The identification method comprises the steps: firstly, carrying out PCR amplification on a cytochrome b gene segment in the chondriosome DNA of a sample of four geographic groups to be detected by using the pair of amplification primers; and secondly, purifying the amplification primer and sequencing, wherein a sample to be detected with the 528-locus and the 534-locus bases of the nucleotide sequences to G is from Fujian group. The invention has the characteristics of stable amplification result, favorable repeatability and simple and effective identification method.

The invention discloses a method for controlling prawn hepatopancreas necrosis by polyculture of scatophagus argus and marsupenaeus japonicas. The method comprises: in breeding, throwing marsupenaeus japonicas shrimp seeds whose total length is 0.8-1.0 cm, throw-in density being 70-80 thousands seeds in each mu, when the shrimp seeds growing to 2-3 cm of marsupenaeus japonicas body length, throwing scatophagus argus whose body length is 3-5 cm, throw-in volume being 200-300 fish / mu. Through throwing the scatophagus argus which has omnivory and benthic feeding habit in proper density, proper specification, prawn culture pond water body and bottom pollution can be reduced, so as to improve culture pond environment, reduce harmful bacteria contents in the pond water and bottom mud, and control occurrence and diffusion of bacterial diseases in prawn culture. The method can effectively eliminate redundant nutrient substances in the bottom of the pond and in the water, reduces bacteria number, prevents outburst of prawn hepatopancreas necrosis, and improves success rate of culture.

Provided is a grading and optimizing method for out of water enduring performance of marsupenaeus japonicas shrimp seeds. The method comprises: substituting the following form scale value of a juvenile prawn into the following computational formula: P=(6503.274C4-9362.831C11+6388.296C15+1595.804C16-2938.016) / (6390.726C4-9133.015C11+6287.753C15+1610.935C16-2921.642); averaging the P values of all shrimp seeds, to obtain an average P value of each pond of shrimp seeds, and according to the average P value, determining values of out of water enduring performance of the shrimp seeds.

The invention provides a method for breeding marsupenaeus japonicas by fluoxetine. The method includes workshop treatment, preparation of a culture pond, spreading of young marsupenaeus japonica, dividing, feeding, water changing and capture. The method has the advantages that according to living habits of the marsupenaeus japonicas, a certain amount of the fluoxetine is added into baits to inhibit the habit of submerging in sand, so that sand is not laid during breeding of the marsupenaeus japonicas, manpower and material resources wasted in sand laying can be saved, and shrimp diseases induced by sand laying can be reduced; through scientific and reasonable spreading density, bait feeding amount and daily management, growth of the marsupenaeus japonicas is accelerated, and the survival rate and weight gain rate in cultivation are increased; the success rate of industrialized breeding of the marsupenaeus japonicas can be ensured, a novel breeding mode under artificial conditions is provided, and the method is beneficial to large-scale breeding and promotion and has a wide application prospect.

The invention discloses a pond multi-stubble mixed culture method for increasing the yield of marsupenaeus japonicus and belongs to the technical field of aquaculture. The method comprises the steps of 1, selection and treatment of a pond; 2, selection and putting of young shrimps; 3, selection of young crabs; 4, daily water quality management; 5, feeding management; 6, timely fishing. In summary,according to a disclosed multi-stubble mixed culture mode of marsupenaeus japonicus and portunus trituberculatus, wherein portunus trituberculatus can ingest the corpses of marsupenaeus japonicus inthe mixed culture process of marsupenaeus japonicus and portunus trituberculatus, and therefore further spreading of shrimp diseases can be effectively prevented. The cultivation procedures are scientific and reasonable, the yield and survival rate of marsupenaeus japonicus can be effectively increased, and the economic benefits of marsupenaeus japonicus and portunus trituberculatus are both increased.

The invention relates to a factory-like large-scale seed culture device and method for marsupenaeus japonicus, and belongs to the technical field of prawn farming. The method includes the following processing steps that 1, a culture pond is constructed; 2, sanding is conducted; 3, a gas-lift unit is installed; 4, disinfecting is carried out; 5, inflow is performed; 6, seeds are put in; 7, the water color is adjusted; 8, bait is thrown; 9, the water quality and growth conditions are monitored; 10, the seeds are moved out of the pond. According to the method, factory-like high-density and large-scale (about 3 cm) seed culture can be conducted, the culture period in the culture pond is shortened, and the large-scale seed culture device and method for the marsupenaeus japonicus which is cultured for multiple batches in one year are realized. By the adoption of the method, the body length of the seeds is cultured to be about 3 cm from 0.8 cm, and the survival rate of the marsupenaeus japonicus can reach 80%; the marsupenaeus japonicus is cultured into commercial sized marsupenaeus japonicus from the body length of about 3 cm, the survival rate of the marsupenaeus japonicus can reach 70%, and the yield can reach 1500-2250 kg / hm2.

The invention discloses a breeding device for polyculture of marsupenaeus japonicus and sea grapes and a breeding method adopting the device. The device comprises a breeding pond, sand is laid on thebottom of the breeding pond, and a plurality of sea grape breeding devices, a plurality of nano oxygenation devices and a water outlet are arranged on the bottom of the breeding pond; and a rope is arranged above the breeding pond, the sea grape breeding devices are connected with the rope through a lifting rope, a floater is arranged on the lifting rope, and a valve is arranged on the water outlet. According to biological characteristics of the marsupenaeus japonicus and the sea grapes, the curved-surface sea grape breeding devices are applied to inoculate the sea grapes, the three-dimensional structure of the curved-surface sea grape breeding devices effectively widens the breeding space of the sea grapes, the growth range of the sea grapes is widened, a good hiding environment is provided for the marsupenaeus japonicus, and the survival rate and the breeding yield of the marsupenaeus japonicus are increased. The breeding device is easy to operate, low in breeding cost, capable of saving bait, good in breeding benefit, suitable for breeding in soil ponds, mulching film breeding ponds and indoor workshops, capable of being popularized in a large range and high in practical value and has wide application prospects.

The invention discloses a microsatellite triple ternary PCR detection method of marsupenaeus japonicas. The method includes the steps that three pairs of PCR primers are designed and synthesized; the total DNA is extracted; combination, a system and a reaction program of the ternary PCR reactions are provided; PCR products are detected. The efficiency of the microsatellite triple ternary PCR detection method of marsupenaeus japonicas can be tripled compared with an original method. The method has the advantages that the cost and time are saved, and the method is efficient, economical, high in repeatability, simple, feasible, beneficial to detection and the like and can be applied and popularized with respect to breeding management, family identification, individual recognition, genetic relationship identification, improved breed breeding, linkage map construction, genetic diversity detection and the like of marsupenaeus japonicas.

The invention provides a method for replanting waste spermatophore of marsupenaeus japonicus, relating to a method for artificial propagation of marine shrimps. The method for replanting waste spermatophore of marsupenaeus japonicus, which is provided by the invention, comprises the following steps: stripping the original complete spermatophore from the seminal receptacle of the female broodstockof the marsupenaeus japonicus which just dies and molts, and cleaning the spermatophore for later use; and taking the female broodstock of the marsupenaeus japonicus the carapace of which is not hardened, sticking a bonding agent at the bottom of the spermatophore, filling the spermatophore into the seminal receptacle of the female broodstock, and then, sterilizing the female broodstock for culturing. The method provided by the invention has simple and understandable principles and is simple and convenient in operation, and the maturing production process of the female broodstock can be carried out at any time according to the needs; and the used bonding agent has wide material sources and is economical and practical. The invention fills the blank of artificial mating of the molted femalebroodstock of the marsupenaeus japonicus in natural sea areas, and has the advantages of very low risk factor in application and obvious economical benefit.

The invention discloses a filter bed breeding system and a factory breeding method of buried-dwelling marine organisms, and belongs to the field of aquiculture. The breeding system comprises a breeding pond, an empty space is arranged at the bottom of the breeding pond, a sand layer filter bed is arranged on the empty space, and a breeding water layer is above the sand layer filter bed; the breeding pond is internally provided with an air-life tube, one end of the air-lift tube communicates with the bottom of the breeding pond, and the other end of the air-lift tube communicates with the breeding water layer; a first oxygen increasing tube is arranged at the bottom of the breeding pond; and a second oxygen increasing tube is arranged at the bottom of the breeding water layer. S and divinghabits of the buried-dwelling marine organisms such as Marsupenaeus japonicus and Babylonia can be satisfied, the problem of bottom substrate changing black during high-density breeding is advantageously solved, the adhesion and purification effect of probiotics are improved, breeding stability is improved, the filter bed breeding system and the factory breeding method of the buried-dwelling marine organisms are suitable for high-density factory breeding of the buried-dwelling marine organisms, and has a good application prospect.

The invention relates to an application of antisense nucleic acid AMO-miR-307 to preparation of a preparation for resisting white spot syndrome viruses. The sequence of the antisense nucleic acid AMO-miR-307 is 5'-CUCACUCAAGGAGGUUGUGA-3'. The antisense nucleic acid AMO-miR-307 has notable resisting effects on WSSV. Host hemolymph cells are promoted to be in apoptosis so as to restrain the proliferation of viruses, and effectively restrain proliferation of WSSV in scylla paramamosain, marsupenaeus japonicus and procambarus clarkii. The antisense nucleic acid AMO-miR-307 provides a potential medicine for preventing and treating the white spot syndrome viruses, so that the infection and the propagation of the white spot syndrome viruses can be effectively prevented and treated, and the antisense nucleic acid AMO-miR-307 is broad in application range, is suitable for marsupenaeus japonicus and the scylla paramamosain which are bred in seawater, and is also suitable for the procambarus clarkii bred in fresh water. The antisense nucleic acid AMO-miR-307 is green and environmental-friendly, cannot influence ecological balance in breeding process, and cannot cause damage to health of humanbodies.

Provided is a grading and optimizing method for ammonia nitrogen resistance of marsupenaeus japonicas shrimp seeds. P=(-374237.401C2+60262.529C4-482.009C6-5837.357C10+2728.598C13+161295.154C14+6521.543C15+575.301C16+7363.539C17-15438.198) / (-373239.473C2+59971.405C4-204.015C6-5345.370C10+2773.543C13+160584.988C14+6394.044C15+566.017C16+7468.213C17-15349.352). The method determines and screens out shrimp seeds in good ammonia nitrogen resistance according to the value of P.

The invention provides a method for accurately and quickly identifying two morphological variants of marsupenaeus japonicus, which relates to the marsupenaeus japonicus. The method comprises the following steps of: designing and amplifying primers of cytochrome b gene segments of the marsupenaeus japonicus; and extracting the DNA of the variants I and II of the marsupenaeus japonicus, performing polymerase chain reaction (PCR) by taking the DNA as a template, performing enzyme cutting on PCR products and performing electrophoresis identification on an enzyme-cutting products, wherein the variant I of the marsupenaeus japonicus has the enzyme cutting sites of gatatc and produces the two enzyme-cutting segments of 284bp and 299bp by the enzyme cutting; and the variant II of the marsupenaeusjaponicus does not have any enzyme-cutting site and still has the strip of 583bp, so that the two variants of the marsupenaeus japonicus can be identified.

The invention relates to a flow velocity resistance grading and optimal-selection method for juvenile Marsupenaeus japonicus. The method comprises the steps: determining C1, C3, C7 and C13 corresponding values of each juvenile Marsupenaeus japonicus, and substituting the corresponding values into a computing formula as follows: P=(9562.935C1+35470.811C3+19125.274C7+4477.221C13-3162.925) / (9791.617C1+34472.473C3+18545.578C7+4522.143C13-3140.599); and averaging obtained P values of each juvenile Marsupenaeus japonicus so as to obtain the average P value of juvenile Marsupenaeus japonicus of each pond, judging the quality grade of the juvenile Marsupenaeus japonicus according to the average P value, and screening out high-quality juvenile Marsupenaeus japonicus with high flow velocity resisting capability.

The invention discloses a portable transport device and a transport method for prawn prawns caught in the sea, characterized in that the device includes a constant temperature box, and the interior of the constant temperature box is divided into three chambers: a shrimp storage room, an ice storage room and equipment In the shrimp storage room, there are shrimp grids for storing shrimp, in the ice storage room, there is a refrigerant, in the equipment room, there is a controller, and between the equipment room and the shrimp storage room, there is a device for circulating and filtering the water in the shrimp storage room. The water filtration system and the oxygen supply system used to supply oxygen to the shrimp storage room, the ice storage room is connected to the oxygen supply system through pipelines, and also includes a temperature control system electrically connected to the controller. The temperature control system uses It is used to detect the water temperature in the shrimp storage room and control the connection or closure of the pipeline according to the water temperature. The advantages are: it can always maintain a constant temperature, reduce the number of operations during the transportation of the shrimp, thereby reducing the emergency response of the shrimp during high temperature, and improving the survival rate of the shrimp. Ensure the vitality of the shrimp.

The invention discloses a multi-crop polyculture method in ponds for increasing the yield of Penaeus japonicus, and belongs to the technical field of aquaculture. The above method includes: step 1: pond selection and treatment; step 2: shrimp seed selection and release; step 3: crab seed selection; step 4: daily water quality management; step 5: feeding management; step 6: timely fishing. In summary, this application discloses the multi-crop polyculture model of Penaeus trituberculatus and Portunus trituberculatus. During the polyculture of Penaeus trituberculatus and Portunus trituberculatus, Portunus trituberculatus will eat the carcasses of Penaeus trituberculatus, which can effectively prevent shrimp diseases further dissemination; the breeding steps are scientific and reasonable, which can effectively increase the yield and survival rate of Penaeus japonicus, and the two together increase economic benefits.