Method for replanting waste spermatophore of marsupenaeus japonicus
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A technology for fine pods and prawns is applied in the field of artificial reproduction of marine prawns and achieves the effects of wide sources, low risk coefficient and significant economic benefits.
Inactive Publication Date: 2012-11-14
XIAMEN UNIV
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However, although the seminal vesicles of the genus Marsupenaeus are also closed, due to the particularity of the morphological structure and unique reproductive habits, this genus only has a single species of Penaeus japonicus. A report on the method of re-implanting the old sperm pods of dead and freshly moulted female broodstock into the nanosperm vesicle
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Embodiment 1
[0017] The condition of embodiment 1 molting female brood shrimp sperm pod replanting promoting maturation domestication pond
[0018] It is equivalent to the broodstock maturation pond, with an area of 20-30m 2 , The seawater is filtered through the conventional shrimp pond inlet filter facilities, the water level is 40-60cm, the salinity is 28-30, the water temperature is 22-25°C, the pH value is 7.8-8.5, the light intensity is 1000lx, and a small amount of aeration.
Embodiment 2
[0019] The source of embodiment 2 waste sperm pods and stripping and preservation
[0020] Carefully peel off the original intact spermatozoa from the nanosperm vesicles of freshly dead and newly molted female broodstock, rinse them with high-pressure sterilized seawater; place the rinsed spermatozoa on wet gauze soaked in sterilized seawater Cover with wet gauze, store in a large petri dish, then place the large petri dish in an incubator, keep the temperature at 18-22°C, and set aside. Generally, it can be stored for 7 days.
Embodiment 3
[0021] Embodiment 3 sperm pod replantation
[0022]Prepare the following materials: pure cotton gloves, gauze, filter paper, surgical tweezers, hand net, 25L plastic bucket; sperm pod binder, iodine powder, oxytetracycline; preparation of seawater containing 50-100ppm iodine for disinfection; maturation and domestication Sprinkle 5-10ppm oxytetracycline in the pool.
[0023] Rinse with sterilized seawater, and then blot dry with filter paper; hold the female brood shrimp whose carapace has not yet hardened firmly with wet gloves on hand, with the belly of the brood upward, and blot the seminal vesicle with filter paper; Gently insert the seminal vesicles of the female shrimp into the seminal vesicles, pinch the seminal vesicles with your fingers, and wait for a few seconds; soak the broodstock shrimps that have just been implanted with seminal pods in seawater containing 50-100 ppm iodine to disinfect and prevent infection, and then put them in In seawater; it is best to be a...
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Abstract
The invention provides a method for replanting waste spermatophore of marsupenaeus japonicus, relating to a method for artificial propagation of marine shrimps. The method for replanting waste spermatophore of marsupenaeus japonicus, which is provided by the invention, comprises the following steps: stripping the original complete spermatophore from the seminal receptacle of the female broodstockof the marsupenaeus japonicus which just dies and molts, and cleaning the spermatophore for later use; and taking the female broodstock of the marsupenaeus japonicus the carapace of which is not hardened, sticking a bonding agent at the bottom of the spermatophore, filling the spermatophore into the seminal receptacle of the female broodstock, and then, sterilizing the female broodstock for culturing. The method provided by the invention has simple and understandable principles and is simple and convenient in operation, and the maturing production process of the female broodstock can be carried out at any time according to the needs; and the used bonding agent has wide material sources and is economical and practical. The invention fills the blank of artificial mating of the molted femalebroodstock of the marsupenaeus japonicus in natural sea areas, and has the advantages of very low risk factor in application and obvious economical benefit.
Description
technical field [0001] The invention relates to a method for artificial propagation of marine shrimps, in particular to a method for re-implanting the original waste spermatozoa of the newly-molted female broodstock of Penaeus japonicus into the nanosperm vesicle of the newly-molted female broodstock. Background technique [0002] Japanese sac shrimp (Marsupenaeus japonicus) is one of the economical shrimps cultured in seawater in my country. It is distributed in the shallow water areas along the southern Yellow Sea, Taiwan, and northern South China Sea. Litopenaeus vannamei), Penaeus monodon (commonly known as grass shrimp) (Penaeus monodon) and Chinese prawn (Fennerpenaeus chinensis); it has relatively high salinity requirements, up to 34, and the ecological habits of Litopenaeus vannamei It has strong complementarity, so it is suitable for breeding in autumn and winter in southern China and summer and autumn in northern my country. Although the culture boom of Litopenaeus ...
Claims
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