26 results about "Hemolymph" patented technology

Uses are described for hemolymph, contents of hemolymph, fragments or components of the contents found within hemolymph, including hemocyanin, any of which may be derived from crustaceans, or recombinantly produced, as functional agents to treat diseases, provide symptomatic relief, provide a beautifying or relieving effect to the intended organ or site-of-intended-use, or be used as an ingredient in formulations designed to help regenerate, repair, or restore the function of the skin barrier, for applications falling within skin barrier restoration, wound healing and anti-aging effects.

The invention belongs to the technical field of shellfish hemolymph collection, and discloses a bivalve shellfish hemolymph collection device. The device comprises a fixing device and a collecting device, the fixing device is composed of a base, a supporting rod, an operating rod, a control handle, a threaded rod, a moving block and a tool bit, shellfish organisms can be effectively fixed and opened, hemolymph can be conveniently collected, the collecting device comprises a vacuum tube and a bidirectional needle head, and the collecting device automatically extracts the hemolymph according to the vacuum negative pressure principle. The shellfish collecting device is simple in structure and convenient to operate, manual operation is not needed, the collecting efficiency and convenience are improved, the problems that in the prior art, shellfishes are fixed unstably, and operation is inconvenient are solved, and the device is simple in structure, convenient to operate, capable of effectively avoiding sample pollution and high in practicability and popularization value. The device is suitable for shellfish hemolymph sampling.

The present invention provides a cell culture dish for crayfish hemolymph experiments. The limiting component includes a snap ring, a rubber body, a pipe, a rotating sphere, an inclined rod, and a clamping block. The snap ring is arranged inside the frame, the rubber body is embedded in the inner wall of the snap ring, the pipe penetrates through the inside of the snap ring, the rotating sphere is hinged to the inner wall of the pipe away from the rubber body, the inclined rod swings at one end of the rotating sphere, the clamping block slides at one end of the inclined rod, and the air inside the rubber body quickly enters the inside of the pipe, facilitating the rapid compression of the air to impact the side surface of the inclined rod. At this time, the inclined rod can swing at an angle through the rotating sphere, and the inclined rod assists the clamping block to slide horizontally to the outside of the pipe. The clamping block can be clamped inside the groove on the outside of the dish body. At this time, the clamping block can be used to limit the dish body, avoiding the situation of the dish body shaking when the whole frame moves.

The invention discloses application of RNA (Ribonucleic Acid) interference on a hepialus armoricanus larva CYP18A1 gene in promoting transformation of cordyceps sinensis from bud spores to hyphae. In order to solve the problems of long artificial tending period, high cost and the like of cordyceps sinensis caused by slow rigidification process of hepialus armoricanus larvae infected by cordyceps sinensis, the siRNA synthesized by related genes (flash, larva of larvae of hepialus armoricanus LCP-30, 26-hydroxylase, isoform B and probe citase 3) acts on the hepialus armoricanus larvae, so that the cordyceps sinensis in hemolymph of the hepialus armoricanus is promoted to be converted into hyphae from blastospores, and the cordyceps sinensis in the hemolymph of the hepialus armoricanus is promoted to grow into the cordyceps sinensis in the hemolymph of the hemolymph of the hemolymph of the hemolymph of the hemolymph of the hemolymph. The result shows that the related gene of the hepialus armoricanus larva can significantly promote the two-form transformation of the cordyceps sinensis, so that the bombyx batryticatus rate of the bacterium-carrying hepialus armoricanus larva is improved.

The invention discloses application of RNA (Ribonucleic Acid) interference on a hepialus armoricanus larva LCP-30 gene in promoting conversion of cordyceps sinensis from bud spores to hyphae. In order to solve the problems of long artificial tending period, high cost and the like of cordyceps sinensis caused by slow rigidification process of hepialus armoricanus larvae infected by cordyceps sinensis, the siRNA synthesized by related genes (flash, larva of larvae of hepialus armoricanus LCP-30, 26-hydroxylase, isoform B and probe citase 3) acts on the hepialus armoricanus larvae, so that the cordyceps sinensis in hemolymph of the hepialus armoricanus is promoted to be converted into hyphae from blastospores, and the cordyceps sinensis in the hemolymph of the hepialus armoricanus is promoted to grow into the cordyceps sinensis in the hemolymph of the hemolymph of the hemolymph of the hemolymph of the hemolymph of the hemolymph. The result shows that the related gene of the hepialus armoricanus larva can significantly promote the two-form transformation of the cordyceps sinensis, so that the bombyx batryticatus rate of the bacterium-carrying hepialus armoricanus larva is improved.

The invention discloses an application of RNA (Ribonucleic Acid) interference of a hepialus armoricanus larva probe citinase gene in promotion of transformation of cordyceps sinensis from bud spores to hyphae. In order to solve the problems of long artificial tending period, high cost and the like of cordyceps sinensis caused by slow rigidification process of hepialus armoricanus larvae infected by cordyceps sinensis, the siRNA synthesized by related genes (flash, larva of larvae of hepialus armoricanus LCP-30, 26-hydroxylase, isoform B and probe citase 3) acts on the hepialus armoricanus larvae, so that the cordyceps sinensis in hemolymph of the hepialus armoricanus is promoted to be converted into hyphae from blastospores, and the cordyceps sinensis in the hemolymph of the hepialus armoricanus is promoted to grow into the cordyceps sinensis in the hemolymph of the hemolymph of the hemolymph of the hemolymph of the hemolymph of the hemolymph. The result shows that the related gene of the hepialus armoricanus larva can significantly promote the two-form transformation of the cordyceps sinensis, so that the bombyx batryticatus rate of the bacterium-carrying hepialus armoricanus larva is improved.

The invention relates to an in-vitro culture method and application of anerennial crabs, and belongs to the technical field of aquaculture. The invention provides a culture medium. The culture medium comprises hemolymph of healthy animals and sterile seawater, the volume ratio of the hemolymph of the healthy animal to the sterile seawater is (1-5): (5-9). According to the in-vitro culture method of the crabbidae, the survival time of the crabbidae inoculated into the culture medium for one time is not less than 1 month under the culture system, and when the crabbidae body reaches the optimal state, the culture is transferred to the condition of 2-8 DEG C, so that the survival time of the crabbidae body can be prolonged to be not less than 2 months, and the survival time of the crabbidae body can be prolonged to be not less than 2 months. If the strains are continuously transferred to a fresh culture medium, long-term stable passage of the polypide can be realized, the in-vitro culture system can effectively guarantee supply of the polypide in non-epidemic seasons, and a key technical support is provided for accelerating research and development of quasi-amarerenosis drugs and realizing effective prevention and control of the quasi-amarerenosis.

The present application relates to a kind of methods for establishing cadmium-induced silkworm liver injury model, comprising: according to the proportion of 10 g mulberry leaf and 1 ml concentration 125 mg / L cadmium chloride solution corresponding to every 25 g silkworm body weight, cadmium chloride solution is evenly applied to the back of mulberry leaf and air-dried, and mulberry leaf is treated with clean water as control;Select five age first day silkworm and divide into model group and blank group, under the condition of controlling temperature and humidity, respectively feed after processing mulberry leaf, calculate the amount of mulberry leaf and solution according to the real-time body weight of silkworm before each feeding, every 12 hours once and continuously feed for 5 days;After feeding, fasting for 12 hours, the weight gain rate of two groups of silkworms, AST and ALT activity in hemolymph are determined, and fat body is observed by histological section;When the weight gain rate of model group is significantly lower than blank group, AST and ALT activity is significantly higher than blank group and the pathological characteristics of karyopyknosis and loose tissue appear in fat body, it is determined that model is established successfully.

Uses are described for hemolymph, contents of hemolymph, fragments or components of the contents found within hemolymph, including hemocyanin, any of which may be derived from crustaceans, as functional agents to treat diseases, provide symptomatic relief, provide a beautifying or relieving effect to the intended organ or site-of-intended-use, or be used as an ingredient in formulations designed to aid in the relief of pathologies or states commonly associated with inflammation and dry skin, including, but not limited to eczema, dermatitis, psoriasis, cystitis, asthma, sinusitis, joint pain, as well as other instances of acute and chronic inflammation, and for relief of general symptoms that may be associated with inflammation and dry skin but not linked directly in the intended use, including, but not limited to, redness, itchiness, irritation, muscle or joint fatigue, swelling, hydrating the skin, and reducing transepidermal water loss (TEWL).

The invention discloses an application of RNA (Ribonucleic Acid) interference on a cuticle protein gene of a hepialus armoricanus larva to promotion of transformation of cordyceps sinensis from bud spores to hyphae. In order to solve the problems of long artificial tending period, high cost and the like of cordyceps sinensis caused by slow rigidification process of hepialus armoricanus larvae infected by cordyceps sinensis, the siRNA synthesized by related genes (flash, larva of larvae of hepialus armoricanus LCP-30, 26-hydroxylase, isoform B and probe citase 3) acts on the hepialus armoricanus larvae, so that the cordyceps sinensis in hemolymph of the hepialus armoricanus is promoted to be converted into hyphae from blastospores, and the cordyceps sinensis in the hemolymph of the hepialus armoricanus is promoted to grow into the cordyceps sinensis in the hemolymph of the hemolymph of the hemolymph of the hemolymph of the hemolymph of the hemolymph. The result shows that the related gene of the hepialus armoricanus larva can significantly promote the two-form transformation of the cordyceps sinensis, so that the bombyx batryticatus rate of the bacterium-carrying hepialus armoricanus larva is improved.

The invention discloses a bee cell culture medium and a preparation method thereof, and relates to the technical field of cell culture, and the survival rate (50.2%) and the activity period (90 days) of bee primary cells are remarkably improved by simulating the osmotic pressure (450-500mOsm / kg), pH (4.5-5.0) and disaccharide metabolism characteristics (glucose: trehalose = 1: 4) of bee hemolymph. The APIS culture medium greatly improves the adherence efficiency of primary cells, establishes a novel platform for in-vitro study of virus-ovary interaction, is suitable for in-vitro culture, immortalized induction and virology model construction of bee ovary cells, solves the problem of rapid cell aging caused by mismatch of physiological parameters of traditional culture genes, and has a good application prospect. The method provides key technical support for swarm health regulation and control, and has important scientific significance and application prospects.

This utility model relates to the field of insect hemolymph collection technology, and discloses a leafhopper hemolymph collection device, including a collection tube, a rubber stopper at the opening of the collection tube, a negative pressure cylinder, a liquid collection tube with an insertion needle at one end, and a positioning component for positioning the collection tube. The other end of the liquid collection tube passes through the rubber stopper and is connected to the collection tube. The rubber stopper is tightly fitted to the opening of the collection tube. An air inlet is opened at the upper part of the negative pressure cylinder, and a bent tube is fixedly inserted into the air inlet of the negative pressure cylinder. The bent tube is fixedly inserted into the rubber stopper and is connected to the collection tube. The positioning component includes an upper sleeve with an opening at the lower end, a lower sleeve with an opening at the upper end, and multiple arc-shaped positioning blocks made of elastic rubber. The purpose is to provide a device for collecting leafhopper hemolymph and to solve the problem that existing insect hemolymph extraction devices require insertion and removal of the suction hose and liquid collection hose on the sealing cap during use, resulting in a complex structure and inconvenience in use.

The present invention provides a culture medium for in vitro culturing Exorista sorbillans Wiedemann and a culturing method thereof. The culture medium includes a liquid culture medium and sterile absorbent cotton. The liquid culture medium is a mixed solution of skim milk, sucrose, yeast extract, egg yolk, silkworm hemolymph, and gentamicin sulfate. The culturing method of the present invention mainly includes attracting Exorista sorbillans Wiedemann to lay eggs with live insects at a certain temperature, and culturing Exorista sorbillans Wiedemann larvae in vitro with an optimized artificial culture medium. The present invention uses live insects to collect a large number of Exorista sorbillans Wiedemann eggs, overcoming the problem of difficult acquisition of fly eggs under the condition of no host. At the same time, adding silkworm hemolymph at a specific period to the in vitro culture medium significantly reduces the development time of maggots and improves the adult fly rate of Exorista sorbillans Wiedemann. The parasitic effect of the in vitro cultured tachinid fly obtained by the present invention has no obvious difference from that of the adult fly parasitized in the host. The above materials are widely available, with low costs, and the operation is simple and feasible, and the whole-year culturing and subculture of Exorista sorbillans Wiedemann can be completely realized.

The present invention discloses the application of brown planthopper anchor protein gene NlGPI as a target in the prevention and control of brown planthopper. Based on the transcriptome data of brown planthopper, the present invention clones the full-length cDNA sequence of NlGPI by PCR technology; analyzes its nucleic acid and protein sequence characteristics by bioinformatics means; detects its spatiotemporal expression law by qRT-PCR technology; and explores the biological function of NlGPI by RNAi technology. The present invention finds that NlGPI is closely related to the process of YLS release from brown planthopper to hemolymph, and plays an important role in the growth, development and reproduction of brown planthopper, and can be used as a potential target for the prevention and control of brown planthopper.

The invention discloses an identification method of a bombyx mori sulfur kinin receptor, and relates to the technical field of sulfur kinin receptor identification. IP3 and Ca < 2 + > in cells are rapidly increased due to stimulation of the sulfur kinin, phosphorylation of ERK1 / 2 is remarkably enhanced, the process is sensitive to a G alpha q specific inhibitor, and food consumption and average weight of silkworms are obviously reduced when the silkworms are treated by the synthetic sulfur kinin; when the synthetic thiokinin is injected into the bombyx mori, the trehalose level in hemolymph is remarkably improved, and the effect is remarkably reduced through BNGR-A9dsRNA pretreatment; the sulfur kinin / BNGR-A9 signal channel is used as a key regulator for feeding behavior and hemolymph trehalose homeostasis in bombyx mori, and the effect of the sulfur kinin / BNGR-A9 signal channel in negative control of food intake and positive regulation of energy balance is highlighted.

This invention belongs to the fields of molecular biology, genetic engineering, and protein engineering; specifically, it relates to a Pacifastin serine protease inhibitor PqPI expressed in the venom of the grassland caterpillar wasp, its encoded nucleic acid sequence, and functional studies. This invention provides a Pacifastin serine protease inhibitor PqPI from the grassland caterpillar wasp, which has the amino acid sequence shown in SEQ ID NO:2. This invention also provides the use of the above-mentioned Pacifastin serine protease inhibitor PqPI from the grassland caterpillar wasp: for the preparation of Pacifastin serine protease inhibitors from the grassland caterpillar wasp, which can be used to inhibit the activation of PPO in the hemolymph of grassland caterpillars or cabbage caterpillars.

The application belongs to the technical field of animal model evaluation, and discloses a method for constructing a 5-hydroxymethylfurfural induced oxidative stress model, which comprises the following steps: S1, culturing experimental fruit flies; S2, fruit fly development cycle determination; S3, quantitative analysis of ROS in fruit fly hemolymph; and S4, determination of SOD, CAT enzyme activity and MDA content. The fruit flies are fed with food containing 5-hydroxymethylfurfural to construct an animal model for studying the side effects of accompanying hazards in food, and normal food is used as a control. The antioxidant stress resistance of the fruit flies in vivo and in the intestinal tract is determined after the fruit flies are fed with the food containing 5-hydroxymethylfurfural. The application can be used to establish an oxidative damage animal (fruit fly) model caused by accompanying hazards in fruit fly food and evaluate the model, and establish a high-throughput screening platform for biological active substances for reducing oxidative damage of the body caused by accompanying hazards.

The invention discloses an insect Raf kinase inhibition factor, which is a protein coded by a CvBV28-5 gene of polydDNA (deoxyribonucleic acid) virus of cotesia vestalis, and the amino acid sequence is shown as SEQ ID No.22 or SEQ ID No.8. The invention further discloses a preparation method of the insect Raf kinase inhibition factor. The nucleotide sequence of the gene for coding the inhibition factor of the insect Raf kinase is as shown in SEQ ID NO. 21 or SEQ ID NO. 7. The invention proves that CvBV28-5 interacts with Raf protein of plutella xylostella for the first time, so that the peak value of 20E titer in hemolymph of larvae of plutella xylostella is influenced, the ecdysis process of the larvae of plutella xylostella is prolonged, and a new gene resource is provided for improving the biological control efficiency of parasitic wasps and developing novel transgenic insect-resistant crops.

A novel, alternative, low-cost synthesis process for the in vitro production of carminic acid by using the hemolymph cells of the insect Dactylopius coccus Costa (cochineal scale insect) for use in the dye industry for food, cosmetics, pharmaceuticals and textiles.

The invention establishes an osmotic pressure-nutrition dual-adaptive marine invertebrate primary cell culture method, and systematically reveals and solves two key technical problems of osmotic pressure adaptation mismatch and nutrition supply mismatch generally existing in a marine invertebrate primary cell culture process for the first time. A dynamic osmotic pressure compensation mechanism with universality and adjustability and a species-specific nutrition supply system are constructed. By introducing a gradient osmotic pressure regulation and control module, the osmotic pressure of a culture system can be continuously adjusted within the range of 600-1100 mOsm / L, and the technical bottleneck that the osmotic pressure of a traditional culture medium is fixed and the adaptation range is limited is broken through; meanwhile, by combining a nutrition compound formula taking homologous hemolymph as a core, the metabolic requirements of marine invertebrate cells are accurately matched, and cell damage and death caused by osmotic stress and insufficient nutrition are effectively relieved. Therefore, the survival rate and stability of primary cell culture are remarkably improved, and a primary cell culture method capable of maintaining cell activity and biological functions for a long time is established.

The application provides a promoter for controlling entomopathogenic fungi to highly express in the hemocoel of insects and application thereof. The promoter is specifically expressed in the hyphal stage, that is, the target gene can be driven to be highly expressed only after entering the body of the insect. The application not only provides a new selection for functional gene research and genetic engineering breeding of the pathogenic fungi, but also can effectively ensure the environmental safety of the genetically engineered strain, and eliminate possible factors of the genetically engineered strain affecting the environment.

The present invention discloses the use of genes associated with the larvae of the bat moth to promote the dimorphic transformation of Cordyceps sinensis. This invention addresses the problems of slow rigidification of bat moth larvae infected with Cordyceps sinensis, which results in long artificial cultivation cycles and high costs for Cordyceps sinensis. By applying siRNA synthesized from genes associated with the larvae of the bat moth (flightin, larval cuticle protein LCP-30, 26-hydroxylase, cuticle protein 18.6, isoform B, and probable chitinase3), the siRNAs were applied to the larvae to promote the transformation of Cordyceps sinensis from blastospores to hyphae in their hemolymph. The results demonstrate that the genes associated with the larvae of the bat moth can significantly promote the dimorphic transformation of Cordyceps sinensis, thereby increasing the rigidification rate of the infected bat moth larvae.

The invention discloses a trichogramma gene editing method. The method comprises the following steps: (1) preparing a test bee species and hosts: preparing host tussah eggs; trichogramma is inoculated, and the trichogramma is inoculated for 6-8 hours; the parasitized tussah eggs are clamped in a fruit fly tube to be cultured; (2) preparation of pupae for injection: culturing in an incubator for 9-10 days, and splitting tussah eggs to obtain female pre-pupae of trichogramma; (3) preparing a solution for injection: mixing Cas9 and sgRNA, and incubating to form an RNP compound; (4) micro-injection: injecting the solution into hemolymph; (5) culturing female pupae in vitro after injection; (6) sub-packaging single heads of the eclosion female bees into finger-shaped tubes, and putting corcyra cephalonica egg cards for parasitizing until the offspring drones are all eclosion; and (7) screening mutants: observing mutation phenotypes under a stereoscope, and determining that gene editing succeeds through PCR (Polymerase Chain Reaction) detection. According to the method, the hosts are replaced by the tussah eggs from the rice moth eggs, so that mechanical damage caused in the dissecting process is reduced.

The present invention belongs to the field of biotechnology, and specifically relates to a method for cultivating butter crabs. The present invention regulates the development process of blue crabs into butter crabs through indoor environmental factors, and provides a method for cultivating butter crabs. The method utilizes the high efficiency, convenience, and low cost of regulating indoor environmental factors, can effectively cultivate butter crabs, and can be suitable for large-scale batch production of butter crabs throughout the year. This method only takes a very short time (only 15 to 20 days) to cultivate butter crabs, and has a high conversion rate. In particular, the number of high-grade butter crabs obtained is greater than that of conventional methods. The color of its umbilicus is orange-yellow, the color of the joint membrane (yellow-blue value) can reach 37, the hemolymph fat content can reach 0.22g / 100g, and the hemolymph beta-carotene content can reach 396μg / 100g, which has extremely high edible value and economic value.

The present invention relates to the technical field of pest control, and in particular to recombinant dsRNA-baculovirus, its preparation method and application. The recombinant dsRNA-baculovirus of the present invention comprises a baculovirus AcMNPV and a dsRNA fragment of a gene introduced into the baculovirus AcMNPV (shown in SEQ ID NO. 2). The present invention improves the assembly structure of the recombinant dsRNA-baculovirus, introduces a target gene with a hairpin structure into the baculovirus AcMNPV, and performs insect RNAi based on the AcMNPV expression vector, thereby solving the effect of lepidopteran insect hemolymph components on RNAi efficiency and improving the RNAi efficiency of lepidopteran insects; it helps to solve the problems of double-stranded ribonucleic acid dsRNA off-target, non-target effects and high cost in RNAi applications for pest control, and provides a new model and means for the control of lepidopteran insects.

The invention belongs to the technical field of biology, and particularly relates to a macrobrachium rosenbergii MrBeclin1 gene, recombinant protein and application. The autophagy related gene MrBeclin1 is cloned and identified from macrobrachium rosenbergii, and the autophagy related gene MrBeclin1 encodes proteins of 426 amino acids and has a conservative APG6 structural domain. The gene has the highest expression in hemolymph, is positioned in cytoplasm, and has obviously up-regulated expression in hepatopancreas and gill after being stimulated by aeromonas hydrophila or LPS (Lipopolysaccharide). Functional mechanism research shows that MrBeclin1 interference can cause significant change of autophagy related gene expression and cause serious pathological damage to hepatopancreas and gill. Meanwhile, the recombinant protein can be directly combined with aeromonas hydrophila, citrobacter freundii, staphylococcus aureus and streptococcus iniae, and can specifically inhibit the growth of the first two gram-negative bacteria. The invention systematically clarifies the key effect of MrBeclin1 in immune defense of macrobrachium rosenbergii through dual mechanisms of autophagy regulation and direct bacteriostasis, and provides a new molecular target and theoretical basis for prevention and control of shrimp epidemic diseases.