10 results about "Hemolymph" patented technology

The invention relates to an in-vitro culture method and application of anerennial crabs, and belongs to the technical field of aquaculture. The invention provides a culture medium. The culture medium comprises hemolymph of healthy animals and sterile seawater, the volume ratio of the hemolymph of the healthy animal to the sterile seawater is (1-5): (5-9). According to the in-vitro culture method of the crabbidae, the survival time of the crabbidae inoculated into the culture medium for one time is not less than 1 month under the culture system, and when the crabbidae body reaches the optimal state, the culture is transferred to the condition of 2-8 DEG C, so that the survival time of the crabbidae body can be prolonged to be not less than 2 months, and the survival time of the crabbidae body can be prolonged to be not less than 2 months. If the strains are continuously transferred to a fresh culture medium, long-term stable passage of the polypide can be realized, the in-vitro culture system can effectively guarantee supply of the polypide in non-epidemic seasons, and a key technical support is provided for accelerating research and development of quasi-amarerenosis drugs and realizing effective prevention and control of the quasi-amarerenosis.

The present application relates to a kind of methods for establishing cadmium-induced silkworm liver injury model, comprising: according to the proportion of 10 g mulberry leaf and 1 ml concentration 125 mg / L cadmium chloride solution corresponding to every 25 g silkworm body weight, cadmium chloride solution is evenly applied to the back of mulberry leaf and air-dried, and mulberry leaf is treated with clean water as control;Select five age first day silkworm and divide into model group and blank group, under the condition of controlling temperature and humidity, respectively feed after processing mulberry leaf, calculate the amount of mulberry leaf and solution according to the real-time body weight of silkworm before each feeding, every 12 hours once and continuously feed for 5 days;After feeding, fasting for 12 hours, the weight gain rate of two groups of silkworms, AST and ALT activity in hemolymph are determined, and fat body is observed by histological section;When the weight gain rate of model group is significantly lower than blank group, AST and ALT activity is significantly higher than blank group and the pathological characteristics of karyopyknosis and loose tissue appear in fat body, it is determined that model is established successfully.

This utility model relates to the field of insect hemolymph collection technology, and discloses a leafhopper hemolymph collection device, including a collection tube, a rubber stopper at the opening of the collection tube, a negative pressure cylinder, a liquid collection tube with an insertion needle at one end, and a positioning component for positioning the collection tube. The other end of the liquid collection tube passes through the rubber stopper and is connected to the collection tube. The rubber stopper is tightly fitted to the opening of the collection tube. An air inlet is opened at the upper part of the negative pressure cylinder, and a bent tube is fixedly inserted into the air inlet of the negative pressure cylinder. The bent tube is fixedly inserted into the rubber stopper and is connected to the collection tube. The positioning component includes an upper sleeve with an opening at the lower end, a lower sleeve with an opening at the upper end, and multiple arc-shaped positioning blocks made of elastic rubber. The purpose is to provide a device for collecting leafhopper hemolymph and to solve the problem that existing insect hemolymph extraction devices require insertion and removal of the suction hose and liquid collection hose on the sealing cap during use, resulting in a complex structure and inconvenience in use.

This invention belongs to the fields of molecular biology, genetic engineering, and protein engineering; specifically, it relates to a Pacifastin serine protease inhibitor PqPI expressed in the venom of the grassland caterpillar wasp, its encoded nucleic acid sequence, and functional studies. This invention provides a Pacifastin serine protease inhibitor PqPI from the grassland caterpillar wasp, which has the amino acid sequence shown in SEQ ID NO:2. This invention also provides the use of the above-mentioned Pacifastin serine protease inhibitor PqPI from the grassland caterpillar wasp: for the preparation of Pacifastin serine protease inhibitors from the grassland caterpillar wasp, which can be used to inhibit the activation of PPO in the hemolymph of grassland caterpillars or cabbage caterpillars.

The application belongs to the technical field of animal model evaluation, and discloses a method for constructing a 5-hydroxymethylfurfural induced oxidative stress model, which comprises the following steps: S1, culturing experimental fruit flies; S2, fruit fly development cycle determination; S3, quantitative analysis of ROS in fruit fly hemolymph; and S4, determination of SOD, CAT enzyme activity and MDA content. The fruit flies are fed with food containing 5-hydroxymethylfurfural to construct an animal model for studying the side effects of accompanying hazards in food, and normal food is used as a control. The antioxidant stress resistance of the fruit flies in vivo and in the intestinal tract is determined after the fruit flies are fed with the food containing 5-hydroxymethylfurfural. The application can be used to establish an oxidative damage animal (fruit fly) model caused by accompanying hazards in fruit fly food and evaluate the model, and establish a high-throughput screening platform for biological active substances for reducing oxidative damage of the body caused by accompanying hazards.

A novel, alternative, low-cost synthesis process for the in vitro production of carminic acid by using the hemolymph cells of the insect Dactylopius coccus Costa (cochineal scale insect) for use in the dye industry for food, cosmetics, pharmaceuticals and textiles.

The invention establishes an osmotic pressure-nutrition dual-adaptive marine invertebrate primary cell culture method, and systematically reveals and solves two key technical problems of osmotic pressure adaptation mismatch and nutrition supply mismatch generally existing in a marine invertebrate primary cell culture process for the first time. A dynamic osmotic pressure compensation mechanism with universality and adjustability and a species-specific nutrition supply system are constructed. By introducing a gradient osmotic pressure regulation and control module, the osmotic pressure of a culture system can be continuously adjusted within the range of 600-1100 mOsm / L, and the technical bottleneck that the osmotic pressure of a traditional culture medium is fixed and the adaptation range is limited is broken through; meanwhile, by combining a nutrition compound formula taking homologous hemolymph as a core, the metabolic requirements of marine invertebrate cells are accurately matched, and cell damage and death caused by osmotic stress and insufficient nutrition are effectively relieved. Therefore, the survival rate and stability of primary cell culture are remarkably improved, and a primary cell culture method capable of maintaining cell activity and biological functions for a long time is established.

The application provides a promoter for controlling entomopathogenic fungi to highly express in the hemocoel of insects and application thereof. The promoter is specifically expressed in the hyphal stage, that is, the target gene can be driven to be highly expressed only after entering the body of the insect. The application not only provides a new selection for functional gene research and genetic engineering breeding of the pathogenic fungi, but also can effectively ensure the environmental safety of the genetically engineered strain, and eliminate possible factors of the genetically engineered strain affecting the environment.

The invention discloses a trichogramma gene editing method. The method comprises the following steps: (1) preparing a test bee species and hosts: preparing host tussah eggs; trichogramma is inoculated, and the trichogramma is inoculated for 6-8 hours; the parasitized tussah eggs are clamped in a fruit fly tube to be cultured; (2) preparation of pupae for injection: culturing in an incubator for 9-10 days, and splitting tussah eggs to obtain female pre-pupae of trichogramma; (3) preparing a solution for injection: mixing Cas9 and sgRNA, and incubating to form an RNP compound; (4) micro-injection: injecting the solution into hemolymph; (5) culturing female pupae in vitro after injection; (6) sub-packaging single heads of the eclosion female bees into finger-shaped tubes, and putting corcyra cephalonica egg cards for parasitizing until the offspring drones are all eclosion; and (7) screening mutants: observing mutation phenotypes under a stereoscope, and determining that gene editing succeeds through PCR (Polymerase Chain Reaction) detection. According to the method, the hosts are replaced by the tussah eggs from the rice moth eggs, so that mechanical damage caused in the dissecting process is reduced.

The invention belongs to the technical field of biology, and particularly relates to a macrobrachium rosenbergii MrBeclin1 gene, recombinant protein and application. The autophagy related gene MrBeclin1 is cloned and identified from macrobrachium rosenbergii, and the autophagy related gene MrBeclin1 encodes proteins of 426 amino acids and has a conservative APG6 structural domain. The gene has the highest expression in hemolymph, is positioned in cytoplasm, and has obviously up-regulated expression in hepatopancreas and gill after being stimulated by aeromonas hydrophila or LPS (Lipopolysaccharide). Functional mechanism research shows that MrBeclin1 interference can cause significant change of autophagy related gene expression and cause serious pathological damage to hepatopancreas and gill. Meanwhile, the recombinant protein can be directly combined with aeromonas hydrophila, citrobacter freundii, staphylococcus aureus and streptococcus iniae, and can specifically inhibit the growth of the first two gram-negative bacteria. The invention systematically clarifies the key effect of MrBeclin1 in immune defense of macrobrachium rosenbergii through dual mechanisms of autophagy regulation and direct bacteriostasis, and provides a new molecular target and theoretical basis for prevention and control of shrimp epidemic diseases.