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Shellfish hemolymph cell classification method based on multiple technologies

A blood lymphocyte and classification method technology, applied in the field of shellfish blood lymphocyte classification based on multi-technology, can solve the problems of complicated classification of shellfish blood lymphocytes, difficulty in obtaining a unified standard, etc., to alleviate the problem of pathogenic diseases , the effect of solving the problem of pathogenic diseases

Inactive Publication Date: 2017-02-15
SHANGHAI OCEAN UNIV
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Problems solved by technology

[0004] To sum up, there is an urgent need for a classification method of shellfish blood lymphocytes, and the classification research of shellfish blood lymphocytes can be relatively accurately distinguished by morphological means, avoiding the differences due to the differences between shellfish species and research The difference in methods has caused the complexity of the classification of shellfish blood lymphocytes, and it is difficult to obtain a unified standard
However, there is no report on the classification method of this shellfish blood lymphocyte.

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  • Shellfish hemolymph cell classification method based on multiple technologies

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Embodiment Construction

[0028] The specific embodiments provided by the present invention will be described in detail below in conjunction with the accompanying drawings.

[0029] Please refer to figure 1 , figure 1 It is a schematic flow chart of the method for classifying shellfish blood lymphocytes based on the multiple technology of the present invention. A method for classifying shellfish blood lymphocytes based on multiple technologies, said method comprising the following steps:

[0030] Step S1: The steps of hemolymph collection and total blood count; use a syringe to draw hemolymph from the shellfish adductor muscle, store the collected hemolymph in ice to reduce spontaneous cell agglutination, and then use MultisizerTM3 Coulter Granules counter to determine the concentration and size distribution frequency of cells in the hemolymph;

[0031] Step S2: the step of cell optical microscope analysis; the hemolymph extracted in step S1 is stained, the slide is sealed with a neutral gum mounting ...

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Abstract

The invention relates to a shellfish hemolymph cell classification method based on multiple technologies. The shellfish hemolymph cell classification method comprises following steps: hemolymph collection and total haemocyte counting, cell optical microscope analysis, flow cytometry, transmission electron microscopy analysis, scanning electron microscope analysis, and statistic analysis. The shellfish hemolymph cell classification method is provided based on the prior art; deficiencies are avoided; problems, that complicating of shellfish hemolymph cell classification is caused by the interspecific difference of shellfish and the difference of research methods and unified standards are difficult to achieved, are avoided; and the shellfish hemolymph cell classification method possesses important meaning on shellfish hemolymph cell classification and study on the action of shellfish hemolymph cells in body immunity process.

Description

technical field [0001] The invention relates to a method for classifying shellfish blood lymphocytes, in particular to a method for classifying shellfish blood lymphocytes based on multiple techniques. Background technique [0002] Because shellfish lack specific immunity (acquired immunity or adaptive immunity), its immune defense function depends on related mechanisms of natural immunity (innate immunity). These immune defense mechanisms are mainly performed by the hemolymphocytes of shellfish. In recent years, with the expansion of the scale of economic shellfish farming, shellfish diseases caused by some bacteria or parasites have frequently appeared, causing major economic losses to the shellfish farming industry. Research on the classification, morphology, and structure of shellfish hemolymphocytes As well as their functions, understanding their role in the body's immune process is particularly important for effectively alleviating and solving some pathogenic diseases...

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Application Information

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IPC IPC(8): G01N15/10G01N15/12G01N15/14G01N23/22
CPCG01N15/10G01N15/12G01N15/14G01N23/22G01N2015/1006G01N2015/1019G01N2015/1022
Inventor 王有基吴芳丽吕为群崔帅康胡梦红隋延鸣黄茜枝
Owner SHANGHAI OCEAN UNIV
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