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Method for determining contents of albendazole and metabolites thereof in hemolymph of Bombyx mori by using ultra-fast liquid chromatography/triple-quadrupole tandem mass spectrometry (UFLC-MS/MS)

A technology of triple quadrupole and albendazole, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult sampling of hemolymph, limited sampling volume, low drug concentration, etc., achieve good selectivity, avoid error, fast qualitative effect

Inactive Publication Date: 2014-01-01
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with humans or livestock and poultry animals, the silkworm is small in size, and it is relatively difficult to sample hemolymph, and the sampling volume is limited, the drug concentration is low, and endogenous substances such as inorganic salts, lipids, proteins, and self-metabolites Substance interference puts forward higher requirements for the detection of silkworm tissue materials, and the above methods are not suitable for the detection of drugs in silkworm tissues
At present, there is no report on simultaneous accurate qualitative and quantitative research on the content of albendazole and its metabolites in silkworm hemolymph

Method used

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  • Method for determining contents of albendazole and metabolites thereof in hemolymph of Bombyx mori by using ultra-fast liquid chromatography/triple-quadrupole tandem mass spectrometry (UFLC-MS/MS)
  • Method for determining contents of albendazole and metabolites thereof in hemolymph of Bombyx mori by using ultra-fast liquid chromatography/triple-quadrupole tandem mass spectrometry (UFLC-MS/MS)
  • Method for determining contents of albendazole and metabolites thereof in hemolymph of Bombyx mori by using ultra-fast liquid chromatography/triple-quadrupole tandem mass spectrometry (UFLC-MS/MS)

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Experimental program
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Embodiment Construction

[0027] Materials and methods

[0028] 1.1 Instruments and reagents

[0029] Ultrafast liquid chromatography-triple quadrupole mass spectrometry (UFLC-MS / MS) (Shimadzu, Japan, AB SCIEX, USA), ultrasonic cleaning machine (SB-5200 DT), Milli-Q ultrapure water equipment ( Millipore, USA), Sigma 3k 15 centrifuge, electronic analytical balance, vortex mixer (IKA, Germany), 0.22 μm micropore PTFE Filter membrane (Tianjin Jinteng Experimental Equipment Co., Ltd.), medical disposable sterile syringes, etc.

[0030] ABZ (99.0%) and ABZSO 2 (98.3%) standards were purchased from Dr. Ehrenstorfer GmbH, Germany; ABZSO (≥98%) and ABZSO 2 -NH 2 (98%) were purchased from Sigma-Aldrich, USA and Alfa Aesar, USA, respectively. Acetonitrile, methanol, etc. were chromatographically pure and purchased from Thermo Fisher Scientific and Oceanpak alexative chemical., Ltd., and the rest of the reagents were of analytical grade.

[0031] Standard and test sample preparation

[0032] 1.2.1 T...

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Abstract

The invention discloses a method for determining the contents of albendazole and metabolites thereof in hemolymph of Bombyx mori by using UFLC-MS / MS. The method comprises the following steps: (1) extracting hemolymph of Bombyx mori so as to obtain a to-be-measured sample solution; (2) measuring standard analytical data of standard albendazole and metabolites thereof; (3) injecting the to-be-measured sample solution obtained in the step (1) into an ultra-fast liquid chromatograph for separation and analysis according to a method used in the step (2) and detecting substance signals by using a triple-quadrupole tandem mass spectrometer so as to obtain analytical data of a to-be-measured sample; and (4) comparing the analytical data of the to-be-measured sample obtained in the step (3) with chromatographic analysis data of albendazole and metabolites thereof so as to realize fast qualitative and quantitative determination of albendazole and metabolites thereof in hemolymph of Bombyx mori.

Description

technical field [0001] The present invention relates to the assay method of the content of albendazole and metabolite thereof, relate in particular to a kind of utilizing ultra-fast liquid chromatography-tandem triple quadrupole mass spectrometry (UFLC-MS / MS) to measure albendazole in silkworm hemolymph and the content of metabolites. Background technique [0002] China is the origin of sericulture, with a history of more than 5,000 years, known as the "Oriental Silk Country". Silk and silk products are my country's main foreign exchange-earning products for export, and its production and sales account for about 70% of the world's total. It is the only export commodity that my country can monopolize the world trade market. [0003] Silkworm egg production is the foundation of the sericulture industry. Bombyx mori microparticle disease is a destructive infectious disease to silkworm egg production. Because of its ability to infect embryos, it has become a quarantine object ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 李丽杨琼邢东旭李庆荣肖阳叶明强
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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