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Nilaparvata lugens vitellogenin N-terminal polypeptide (VgNPP) with ovary targeting function and application of nilaparvata lugens VgNPP

A vitellogenin and brown planthopper technology, applied in the direction of DNA / RNA fragments, peptides, peptide sources, etc., can solve the problems of large molecular weight, non-transportable carrier development, difficult target protein fusion expression and purification, and achieve the effect of broad application prospects

Active Publication Date: 2020-05-19
ZHEJIANG UNIV
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  • Abstract
  • Description
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Problems solved by technology

The molecular weight of insect Vg protein is usually relatively large (>150kDa), it is difficult to fuse with the target protein, express and purify, so it cannot be directly used in the development of transport vectors

Method used

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  • Nilaparvata lugens vitellogenin N-terminal polypeptide (VgNPP) with ovary targeting function and application of nilaparvata lugens VgNPP
  • Nilaparvata lugens vitellogenin N-terminal polypeptide (VgNPP) with ovary targeting function and application of nilaparvata lugens VgNPP
  • Nilaparvata lugens vitellogenin N-terminal polypeptide (VgNPP) with ovary targeting function and application of nilaparvata lugens VgNPP

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Embodiment Construction

[0032] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings. It should be understood that these examples are used to illustrate the present invention and not to limit the scope of the present invention. The implementation conditions adopted in the examples can be further adjusted according to the specific application conditions, and the unspecified implementation conditions are usually the conditions in routine experiments. In the present invention, unless otherwise specified, the scientific and technical terms used herein have the meanings commonly understood by those skilled in the art. Moreover, the cell culture, molecular genetics, nucleic acid chemistry, and immunology laboratory operation steps used herein are all routine steps widely used in the corresponding fields. Meanwhile, in order to better understand the present invention, definitions and explanations of relevant term...

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Abstract

The invention relates to an efficient biomolecular efficient delivery technology, and aims to provide a nilaparvata lugens vitellogenin N-terminal polypeptide (VgNPP) with an ovary targeting functionand application of the nilaparvata lugens VgNPP. An amino acid sequence of the polypeptide is shown in SEQ ID NO: 1. According to the application of the polypeptide as a nilaparvata lugens ovary-targeting biomolecule transporter, the nilaparvata lugens VgNPP carries a biomolecule to enter an egg through nilaparvata lugens parent hemolymph in a targeting manner; and the biomolecule is any one of ora complex of more of nucleic acid, oligopeptides, polypeptides, protein, saccharides, lipid, nanoparticles and a small-molecule compound. The popypeptide provided by the invention is used as the biomolecule transporter, particularly as a gene editing element transporter to carry the biomolecule to enter the ovary of the nilaparvata lugens. Compared with other foreign high polymer materials or foreign protein, the VgNPP is derived from the nilaparvata lugens, so that the VgNPP has no toxic or side effects on the nilaparvata lugens and has very broad application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an N-terminal peptide segment of vitellogenin from brown planthopper with ovarian targeting function and its use, and in particular to a technical method for high-efficiency delivery of biomolecules. Background technique [0002] Brown planthopper (Nilaparvata lugens (Stal)), Hemiptera, Planthopperidae, monophagous pest, directly sucks phloem sap of rice and spreads rice virus to harm rice. The outbreak of brown planthopper can lead to "lice burning" of large areas of rice. At present, brown planthopper has become an important pest in rice growth in many Asian countries. Due to its important economic value and ecological significance, for a long time, the main research on brown planthopper has focused on reproductive development, pesticide resistance, wing type differentiation, long-distance migration and so on. The RNA interference technology applied to the study of gene function p...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K19/00C12N15/12C12N15/62A61K47/42
CPCC07K14/43563A61K47/42C07K2319/00
Inventor 徐海君薛文华王琪琦
Owner ZHEJIANG UNIV
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