Application of glycine or metabolism regulator thereof in improving domestic silkworm silk output and method thereof

Abstract

The invention discloses application of glycine or a glycine metabolism regulator, i.e., 1-aminocyclopropane-1-carboxylic acid (ACPC), in improving domestic silkworm silk output, as well as a method for improving the domestic silkworm silk output by supplying exogenous glycine or ACPC to domestic silkworms in a larval period. According to the invention, by researching change in a hemolymph metabolite spectrum of different varieties of domestic silkworms having a large difference in silk output based on GC-MS metabonomics technology, strong correlation is proved to exist between the glycine (42.8% of silk fibroin), as an important component, of domestic silkworm silk fibroin, and a cocoon layer ratio (correlation coefficient is -0.892); the domestic silkworm silk output is significantly improved by intervening metabolism of the silkworms in the larval period through in vitro administration of the glycine or the glycine ACPC; therefore, the invention not only widens application of the glycine or the ACPC in the field of biology, but also provides a new method for improving the silk output in silkworm industry.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of research results of silkworm metabolomics. Background technique [0002] The silkworm (Bombyx mori) belongs to Lepidoptera insects and is a kind of complete metamorphosis insect, which goes through four completely different developmental stages in shape and physiological function including egg, larva, pupa and adult. The silkworm is an oligophagous insect that feeds on mulberry leaves and is a silk-spinning insect with high economic value. As a natural animal protein, silk is mainly composed of silk fibroin and sericin. The types of amino acids that make up silk are relatively concentrated, mainly glycine, serine and alanine. The application of silk is very extensive, not only in the silk industry, but also in the cosmetics industry, pharmaceutical industry and health care products industry. With the increasing development of the commodity economy and the gradual ...

AI Technical Summary

Problems solved by technology

Although the silk production of silkworms has been significantly increased by improving the quality of mulberry leaves and breeding methods, as well as continuous hybridization and improvement of silkworm varieties, it is difficult to continue to increase the silk production of silkworms by relying on traditional breeding methods. Therefore, the development of Other methods to improve the silk production capacity of silkworm are imminent

[0003] At present, the drawing of the silkworm genome framework and fine map has been completed, and the proteomics research of the important tissues and organs of the silkworm has also made great progress, but the research on the small molecule metabolite profile of the silkworm has not been reported yet.

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