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Artificial medium of trichogramma

A culture medium and the technology of Trichogramma, applied in the field of insect breeding, can solve the problems of weak viability, difficulty in continuous cultivation, and low eclosion rate of adults, and achieve the effects of stable cultivation effect, reduced cost, and reduced content

Active Publication Date: 2014-06-18
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the failure to develop an artificial medium that can reproduce Trichogramma in large quantities in vitro, the continuous generation in vitro cultivation technology has not yet made breakthroughs.
In view of the high content of insect matter (pupal hemolymph) in the artificial medium (more than 40%), unstable quality of bees, low adult eclosion rate, weak viability, and difficulty in continuous culture, it is urgent to develop high-quality artificial culture medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The Trichogramma artificial culture medium of this embodiment, based on the total mass fraction of 100%, includes tussah silkworm chrysalis hemolymph 30%, egg yolk 25%, mass fraction 10% skimmed milk 20%, Nissl's salt solution 10%, trehalose 5%, mass fraction 5% Ganoderma lucidum polysaccharide solution 5% and mass fraction 3.6% chitooligosaccharide solution 5%. According to the ratio in the above formula, mix the above materials evenly, which is the Trichogramma artificial culture medium of this embodiment.

[0016] Use the Trichogramma artificial culture medium of the present embodiment to cultivate pine caterpillar Trichogramma in vitro, on average every honeybee card obtains 95% parasitism rate, 100% pupation rate, 84.4% eclosion rate, 89.6% normal bee rate and 911 normal bees Amount of feathering. No population degeneration was found after continuous feeding for more than 25 generations.

[0017] Based on the mass fraction of 100%, the average Trichogramma per T...

Embodiment 2

[0021] The Trichogramma artificial culture medium of this embodiment, based on the total mass fraction of 100%, includes 26.8% of tussah silkworm chrysalis hemolymph, 23.8% of egg yolk, 19.2% of skimmed milk with a mass fraction of 10%, 13.4% of Nissl's salt solution, trehalose 1.8%, mass fraction 5% Ganoderma lucidum polysaccharide solution 12% and mass fraction 3.6% Chitooligosaccharide solution 3%. According to the ratio in the above formula, mix the above materials evenly, which is the Trichogramma artificial culture medium of this embodiment.

[0022] With the Trichogramma artificial culture medium of embodiment in vitro cultivation pine caterpillar Trichogramma, on average every honeybee card obtains 92.0% parasitic rate, 99.2% pupation rate, 76.8% eclosion rate, 82.5% normal bee rate and 450 normal bee emergences quantity. No population degeneration has been found in continuous breeding for more than 20 generations.

[0023] Based on the mass fraction of 100%, use 25%...

Embodiment 3

[0026] The Trichogramma artificial culture medium of this embodiment, based on the total mass fraction of 100%, includes tussah silkworm chrysalis hemolymph 32.5%, egg yolk 28%, mass fraction 10% skimmed milk 18%, Nissl's salt solution 8.2%, trehalose 3.3%, mass fraction 5% Ganoderma lucidum polysaccharide solution 8% and mass fraction 3.6% Chitooligosaccharide solution 2%. According to the ratio in the above formula, mix the above materials evenly, which is the Trichogramma artificial culture medium of this embodiment.

[0027] Use the Trichogramma artificial culture medium of the present embodiment to cultivate pine caterpillar Trichogramma in vitro, and on average every honeybee card obtains 97% parasitic rate, 98.8% pupation rate, 79.4% eclosion rate, 87.5% normal bee rate and 810 normal bee Amount of feathering. No population degeneration has been found in continuous breeding for more than 20 generations.

[0028] Based on the mass fraction of 100%, use 30% of tussah si...

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Abstract

The invention discloses an artificial medium of trichogramma. The artificial medium comprises the following ingredients in mass fraction: 19-40% of insect pupae hemolymph, 17-38% of egg yolk, 10-33% of milk, 4-19% of a Nissl salt solution, 0.8-8% of trehalose, 2-15% of a ganoderan solution with a mass fraction of 5% and 2-15% of a chitosan oligosaccharide solution with a mass fraction of 3.6%. By adopting the artificial medium of trichogramma, the breeding effect of trichogramma is obviously better than that of a traditional artificial medium through the trehalose, the ganoderan solution and the chitosan oligosaccharide solution via effective composition, and the content of insects can be reduced, so that the breeding cost of trichogramma is reduced. Through the artificial medium of trichogramma, trichogramma can be bred by subculturing, no degradation phenomenon exists, and the breeding effect is stable. By adopting the artificial medium of trichogramma, various kinds of trichogramma such as trichogramma dendrolimi, trichogramma confusum, Asian corn borer trichogramma, European corn borer trichogramma and geographical populations thereof can be bred, and good effects are realized.

Description

Technical field: [0001] The invention belongs to the field of insect breeding, and in particular relates to an artificial culture medium for Trichogramma. Background technique: [0002] Trichogramma belongs to Hymenoptera Trichogramma. It is rich in resources and widely distributed. It is an important parasitic natural enemy insect resource. Since the 1920s, it has been widely used in the biological control of various agricultural and forestry pests. It has a remarkable control effect on pests and plays an important role in food safety production, pollution-free food and organic food production. At present, Trichogramma is propagated by eggs of insects such as rice moth, wheat moth, mealworm, tussah silkworm, castor silkworm and pine caterpillar at home and abroad. A prominent problem exploited by eye bees. Therefore, it is particularly important to use artificial medium to raise and breed Trichogramma. [0003] In the 1980s, Chinese scholars successfully used artificial ...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/08A23K1/10A01K67/033A23K10/28
CPCY02P60/87
Inventor 吕欣韩诗畴戴建青叶静文李丽英
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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