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Barley worm natural antibacterial protein and preparation method and application thereof

A natural antibacterial and antibacterial protein technology, applied in the biological field, can solve the problems such as the increase of pathogenic bacteria, and achieve the effect of simple preparation method, high stability and low price

Active Publication Date: 2019-09-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The discovery of penicillin has brought a qualitative leap in the treatment of pathogenic bacterial infection diseases, but due to the abuse of antibiotics, the increase of antibiotic-resistant pathogenic bacteria has become an increasingly serious problem

Method used

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  • Barley worm natural antibacterial protein and preparation method and application thereof
  • Barley worm natural antibacterial protein and preparation method and application thereof
  • Barley worm natural antibacterial protein and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 barley worm natural antibacterial protein

[0041] Include the following steps:

[0042] 1. Select barley worm larvae with a body length of more than 3cm, use water-rich vegetables and fruits as food for free feeding, and raise them until the larvae are more than 4cm long;

[0043] 2. Use a concentration of 10 10 CFU / mL of Escherichia coli (ATCC 25922) bacterial liquid injection to the larvae obtained in step A, the inoculation site is the 4th to 8th internode on the back of the larvae, the inoculation volume of each worm is 2 μL, and then continue to feed for 24 hours;

[0044] 3. Collect the hemolymph of the larvae obtained in step 2 (the hemolymph must be placed on ice during the collection process to prevent the hemolymph from being oxidized and blackened), and then place it in a boiling water bath at 100°C for 5-10 minutes to obtain a solid, and smash the solid , centrifuge at 3000g at 4°C for 5-15min, and collect the supernatant; ...

Embodiment 2

[0051] The preparation of embodiment 2 barley worm natural antibacterial protein

[0052] Include the following steps:

[0053] 1. Select barley worm larvae with a body length of more than 3cm, use water-rich vegetables and fruits as food for free feeding, and raise them until the larvae are more than 4cm long;

[0054] 2. Use a concentration of 10 10 CFU / mL ATCC25923 Staphylococcus aureus bacteria liquid injection of the larvae obtained in step A, the inoculation site is the 4th to 8th internode on the back of the larvae, the inoculation volume of each worm is 2 μL, and then continue to feed for 24 hours;

[0055] 3. Collect the hemolymph of the larvae obtained in step 2. During the collection, the hemolymph must be placed on ice to prevent the hemolymph from being oxidized and blackened, and then placed in a boiling water bath at 100°C for 5-10 minutes to obtain a solid, smash the solid, Centrifuge at 3000g at 4°C for 5-15min, and collect the supernatant;

[0056] Specifi...

Embodiment 3

[0062] Preparation and application of embodiment 3 trauma gel ointment

[0063] This embodiment provides a wound gel ointment, the preparation method of which is as follows: add the freeze-dried powder of the antibacterial protein prepared in Embodiment 1 or 2 into water to prepare a concentration of 0.1mg-4mg / mL, and add 1.5% carbomer Substrate, that is.

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Abstract

The invention provides barley worm natural antibacterial protein and a preparation method and application thereof. According to the barley worm natural antibacterial protein and the preparation method and application thereof disclosed by the invention, escherichia coli and / or staphylococcus aureus are / is used as an inducer, after bacterial liquid is injected to barley worm larvae or yellow mealworm larvae, then the barley worm larvae or the yellow mealworm larvae are raised for some time, hemolymph of the larvae is collected, and antibacterial protein is extracted. The obtained antibacterial protein can be used for preparing an antibacterial agent or a preservative. The antibacterial protein provided by the invention has the characteristics of being good in broad-spectrum antibacterial properties, high in stability, good in safety and the like, the preparation technology is simple, raw materials are cheap and easy to obtain, and the application prospect is great.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to natural antibacterial protein of barley worm and its preparation method and application. Background technique [0002] The discovery of penicillin has brought a qualitative leap in the treatment of pathogenic bacterial infections. However, due to the abuse of antibiotics, the increase of antibiotic-resistant pathogenic bacteria has become an increasingly serious problem. In recent years, there has been a lack of research and development of new antibiotics, and as people have higher and higher requirements for food quality and environmental quality, the use of antibiotics and preservatives has gradually been restricted. It has been found that natural antibacterial protein, especially natural antibacterial protein derived from insects, is a new type of antibacterial and food preservative with great potential. [0003] At present, there are more than 200 kinds of natural insect peptide...

Claims

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Application Information

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IPC IPC(8): C07K14/435A23L3/3526A23K20/147A61K38/17A61P31/04
CPCA23L3/3526A23V2002/00A61K38/1767A23K20/147A61P31/04C07K14/43563A23V2200/10A23V2250/542Y02A50/30
Inventor 王九峰杜孟泽朱要宏郭亮王胜华
Owner CHINA AGRI UNIV
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