32 results about "Prophenoloxidase" patented technology

The invention relates to a preparation method and application of soluble housefly MdproPO1 recombination protein. The preparation method includes the steps of firstly, building a mdproPO1 / pET-30a recombination expression vector; screening the expression strain mdproPO1 / pET-30a / Rosetta of the high-expression MdproPO1 recombination protein; thirdly, performing fermental cultivation and induced expression of the expression strain; fourthly, performing denaturation and renaturation of MdproPO1 inclusion bodies. The preparation method has the advantages that housefly prophenoloxidase (MdproPO1) produced by an Escherichia coli expression system is high in expression quantity, a large amount of MdproPO1 inclusion bodies can be obtained through prokaryote recycling and crushing, and the soluble MdproPO1 recombination protein with enzymatic activity can be obtained through the denaturation and renaturation of the MdproPO1 inclusion bodies; the MdproPO1 recombination protein can be developed into fly spray, an oxidizing agent, a blackening agent and an immunizing agent and is applicable to multiple fields such as sanitation, healthcare, biology and chemical engineering.

The invention discloses a Pteromalus puparum venom Kazal serine proteinase inhibitor polypeptide which has an amino acid sequence represented by SEQ ID NO:2. The invention simultaneously discloses gene coding the Pteromalus puparum venom Kazal serine proteinase inhibitor polypeptide, wherein the gene has a nucleotide sequence represented by a 145-297 nucleotide sequence in SEQ ID NO:1, or has a nucleotide sequence having at least 70% homology with the 145-297 nucleotide sequence in the SEQ ID NO:1, or has a nucleotide sequence which can be hybridized with the 145-297 nucleotide sequence in the SEQ ID NO:1 at 40-55DEG C. The polypeptide of the invention is used for preparing Pteromalus puparum venom Kazal serine proteinase inhibitors, and the inhibitors can be used for inhibiting the activation of Pieris rapae or Papilio xuthus hemolymph PPO (prophenoloxidase).

The invention provides a method for adjusting water quality in shrimp and shellfish polyculture, which belongs to the technical field of aquaculture, including establishing a bacteria and algae synergistic system capable of adjusting water quality, and providing microencapsulated culture bait that can slow down water quality deterioration; the above-mentioned bacteria The algae synergy system includes the algae system that grows on the porous carrier and the biofloc system that adds the carbon source skeleton; the microencapsulated culture bait includes the porous core with lipid attached under vacuum, and the porous core that covers the core. In the film of a biodegradable polymer, the film has a thickness of not less than 1 nm and not more than 1 micron. The method provided by the invention combines the synergistic effect of the bait and the bacteria and algae system to achieve zero water exchange effect, can improve the hydrophobicity of the bait so that it does not disintegrate, reduces the carbon source cost of the biofloc system, and increases the volume concentration and total biofloc concentration. The concentration of suspended solids can increase the content and activity of Omega‑3 fatty acids and immune molecules such as phenoloxidase and hemolysin in the body.

The invention discloses a polyphenol oxidase YHL21 gene and polypeptide sequence. The polyphenol oxidase is confirmed by gene splicing and high-throughput screening of enzyme performance. It has a wide range of temperature resistance and strong high-temperature catalytic performance. Single peptide construction, easy membrane immobilization and other advantages. The present invention also discloses a method for recombinant expression and secretion of polyphenol oxidase YHL21 in Bacillus subtilis strain 168, and a method for using polyvinylidene fluoride (PVDF) membrane to immobilize recombinant polyphenol oxidase on the membrane surface , the membrane-enzyme composite system can stably and efficiently degrade organic substances such as phenol and p-chlorophenol in aqueous solution. The present invention carries out enzyme engineering optimization through gene splicing, and obtains a highly efficient polyphenol oxidase that degrades phenolic pollutants, uses Bacillus subtilis to carry out recombinant secretion expression, and uses PVDF to carry out enzyme immobilization, and improves the enzyme catalytic efficiency.

The invention discloses a method for irradiating oriental fruit flies. The method comprises the step that 60 Co gamma rays of dosage ranging from 40 Gy to 160 Gy are used for irradiating oriental fruit eggs, or first instar larvae and second instar larvae; or 60 Co gamma rays of dosage larger than 160 Gy are used for irradiating third instar larvae of oriental fruit flies. The invention further discloses a method for identifying whether oriental fruit flies are processed by irradiation reaching the standard. Live oriental fruit flies on detected plants or fruits are collected to detect the enzymatic activities of phenol oxidase, superoxide dismutase or / and acetylcholine esterase; if the enzymatic activity of the phenol oxidase is smaller than 80% of that of a contrast, the enzymatic activity of the superoxide dismutase is smaller than 85% of that of a contrast, or / and the enzymatic activity of the acetylcholine esterase is smaller than that of a contrast, it is determined that larvae are irradiated. By measuring the enzymatic activities of the phenol oxidase, superoxide dismutase (SOD) or / and acetylcholine esterase (AChE), whether oriental fruit flies are irradiated or not can be determined, and the larval period of the irradiated oriental fruit flies can be determined. The method is quite sensitive and reliable, and only one oriental fruit fly is needed during identification on the basis of the characteristic that the imported fruit intercept insect quantity is small.

The invention discloses a nematode preparation for preventing and controlling flower slugs, a preparation method and application thereof, and belongs to the technical field of biological pesticides. The nematode preparation of the present invention includes preparation A and preparation B containing different active ingredients, the active ingredient of preparation A includes L-dopa, a substrate inhibitor of parasitic rod nematode and polyphenol oxidase; the active ingredient of preparation B includes polyphenol oxidase Quercetin, a structural inhibitor of phenoloxidase. When applying the nematode preparation of the present invention to prevent and control flower slugs, preparation A is applied to the rhizosphere soil surface of flowers in the evening; preparation B is sprayed on the buds and leaves of flowers in the afternoon of the same day. In the present invention, for the first time, the slug bio-control factor parasitic rod nematode and the two functional inhibitors of slug polyphenol oxidase are used in combination, and the field quick-acting performance of the parasitic rod nematode is greatly improved by using the slug feeding attraction, and through the preparation A and formulation B selected different application locations, application times and methods, which accelerated nematode infestation and lethal slugs.

The invention relates to an application of a cell wall skeleton as a penaeus vannamei feed additive. According to the application, 0.8-1.2g of the cell wall skeleton is added to each kilogram of a penaeus vannamei basal feed, wherein the cell wall skeleton is obtained in the following manner of enabling separated and purified nocardiopsis lucentensis to be subjected to enlarged culture, performingultrasonic wave crushing on fermentation liquor, performing centrifuging, performing enzymolysis with trypsin, performing defatting, and performing freeze drying so as to obtain a white cell wall skeleton. According to the application disclosed by the invention, the activity of superoxide dismutase, nitricoxide synthase, phenoloxidase, lysozyme and glutathione peroxidase in hemolymph and hepatopancreas of penaeus vannamei can be notably increased, the concentration of malondialdehyde can be notably reduced, the respiratory burst activity of the hemolymph of the penaeus vannamei can be notablystrengthened, the immunity protection ratio of the penaeus vannamei can be obviously increased, and the breeding survival rate of the penaeus vannamei can be obviously increased.

The invention belongs to the technical field of molecular biology, and specifically relates to a C1q receptor PtgC1qR gene of Portunus trituberculatus and its encoded protein and application. The C1q receptor PtgC1qR gene of Portunus trituberculatus is shown in the base sequence of SEQ ID No.1 in the sequence table. The cDNA of PtgC1qR gene was amplified from Portunus trituberculatus by cDNA library and RACE technology, and the recombinant PtgC1qR protein had significant phenoloxidase inhibitory activity, antibacterial activity and microbial binding activity. The invention lays a foundation for disease prevention and control of portunus trituberculatus and gene-assisted selective breeding, and has potential application value in the development of aquatic medicines and the production of feed additives.

The invention relates to the technical field of feed additives, in particular to a compound microbial preparation and its application in post-spraying of aquatic feed. The compound microbial preparation includes Enterococcus faecalis, Clostridium butyricum, Bacillus licheniformis and a compound protective agent, which are added to aquatic feed through a post-spraying process, which can effectively reduce the activity loss of microorganisms in the process of feed processing and feeding, thereby making the The survival rate and weight gain rate of turbot were significantly improved, and the weight gain rate increased by 19.8%, while the feed factor decreased by 14.3%. The activities of phenoloxidase and lysozyme in the blood of turbot in the experimental group increased respectively. 76.5% and 64.4%, which is beneficial to reduce the cost of feed, improve the immunity of aquatic animals, increase the breeding benefits, and the effect is remarkable.