Composite micro-ecological preparation and application thereof

It is a technology of compound microecology and preparation, which is applied to bacteria and lactobacilli used in application and food preparation to achieve the effect of improving the level of immunity and raising the level of breeding animals.

Pending Publication Date: 2020-04-28
福建御研农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no product using Lactobacillus johnsonii as a feed additive in China, and its application in aquaculture is rarely reported

Method used

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  • Composite micro-ecological preparation and application thereof
  • Composite micro-ecological preparation and application thereof
  • Composite micro-ecological preparation and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0028] The preparation method of compound probiotics is as follows:

[0029] 1. Preparation of Lactobacillus johnsonii microcapsules

[0030] 1) Activate the cryopreserved Lactobacillus johnsonii three times on the MRS agar plate, pick a single colony and inoculate it into a 10mL deep layer MRS liquid test tube, cultivate it at 37°C for 10 hours, then transfer it to 250mL MRS liquid medium with 1% inoculation amount, After cultivating for 8-10 hours, it is used as seed liquid;

[0031] 2) Inoculate the seed solution obtained in step 1) in a 5L fermenter with an inoculum amount of 5% to 10% for cultivation; the fermentation medium is MRS liquid medium;

[0032] 3) During the fermentation process, the pH value of the fermentation broth is maintained at 6.0-6.5. When the pH exceeds the set value, feed liquid is added; when the pH is lower than the set value, alkaline neutralizers such as ammonia, KOH, and NaOH are automatically added. Any one; ferment for 10 to 14 hours at a te...

Embodiment 1

[0050] The preparation method of compound probiotics is as follows:

[0051]1. Preparation of Lactobacillus johnsonii microcapsules

[0052] 1) Activate the cryopreserved Lactobacillus johnsonii three times on the MRS agar plate, pick a single colony and inoculate it into a 10mL deep layer MRS liquid test tube, cultivate it at 37°C for 10 hours, then transfer it to 250mL MRS liquid medium with 1% inoculation amount, After cultivating for 10 hours, it was used as a seed solution;

[0053] 2) Inoculate the seed solution obtained in step 1) in a 5L fermenter with an inoculum size of 5% for cultivation; the fermentation medium is MRS liquid medium;

[0054] 3) During the fermentation process, the pH value of the fermentation broth is maintained at 6.0. When the pH exceeds the set value, the feed liquid is added; when the pH is lower than the set value, the alkaline neutralizer ammonia water is automatically added; Ferment for 14 hours under the condition of 100rpm, N 2 , to mai...

Embodiment 2

[0072] The preparation method of compound probiotics is as follows:

[0073] 1. Preparation of Lactobacillus johnsonii microcapsules

[0074] 1) Activate the cryopreserved Lactobacillus johnsonii three times on the MRS agar plate, pick a single colony and inoculate it into a 10mL deep layer MRS liquid test tube, cultivate it at 37°C for 10 hours, then transfer it to 250mL MRS liquid medium with 1% inoculation amount, After cultivating for 8 hours, it was used as a seed solution;

[0075] 2) Inoculate the seed solution obtained in step 1) in a 5L fermenter with an inoculum size of 8%; the fermentation medium is MRS liquid medium;

[0076] 3) During the fermentation process, the pH value of the fermentation broth is maintained at 6.5. When the pH exceeds the set value, the feed liquid is added; when the pH is lower than the set value, the alkaline neutralizer ammonia water is automatically added; Ferment for 12 hours under the condition of 100rpm, N 2 , to maintain dissolved ...

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Abstract

The invention provides a composite micro-ecological preparation. The composite micro-ecological preparation is composed of a lactobacillus johnsonii microcapsule, ginseng extract and Chinese angelicaextract. The invention also provides an application of the above composite micro-ecological preparation in preparation of feed for improving an immune level of farmed animals. The composite micro-ecological preparation provided by the invention can improve the immunity level of the farmed animals, and improve the activity of alkaline phosphatase (AKP enzyme), superoxide dismutase (SOD enzyme) andphenol oxidase (PO enzyme) in serum and hepatopancreas of the farmed animals, thereby having great significance for improving the breeding level of the farmed animals in China.

Description

technical field [0001] The invention belongs to the technical field of feed additives, and in particular relates to a compound microecological preparation and an application thereof. The compound microecological preparation can significantly improve the immune level of farmed animals. Background technique [0002] The abuse of antibiotics has brought a series of side effects to the aquaculture industry. Antibiotics are being banned from being used in aquaculture at home and abroad. Therefore, seeking alternatives to antibiotics has become the primary topic of research by scholars at home and abroad. Since the compound microecological preparation has the advantages of multiple functions, no toxic side effects, no residue, and no environmental pollution, it has become one of the more ideal substitutes. As a multifunctional microecological additive, compound probiotics have been widely used in the field of aquaculture. It is mainly composed of live microorganisms and prebiotic...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/30A23K50/80
CPCA23K10/18A23K10/30A23K50/80A23V2400/151
Inventor 刘宝玉
Owner 福建御研农业科技有限公司
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