The invention provides a liver-protecting and anti-fatigue composition, which is characterized in that American ginseng saponin, astragalus polysaccharide, lycium barbarum polysaccharide, black funguspolysaccharide and black tea powder in the composition interact with one another through a proper weight ratio, are matched together and have a synergistic effect, so that the anti-fatigue, anti-oxidation and liver-protecting effects are improved together; after the components are combined, the weight-bearing swimming time of mice can be prolonged, the glycogen storage and antioxidant enzyme activity can be improved, the content of serum lactic acid, urea nitrogen and liver malonaldehyde can be reduced, and an obvious liver protection effect can be achieved. According to the liver-protectingand anti-fatigue composition provided by the invention, taurine, lysine, inositol, nicotinic acid, vitamin B6 and vitamin B12 are added on the basis of American ginseng saponin, astragalus polysaccharide, lycium barbarum polysaccharide, black fungus polysaccharide and black tea powder, anti-fatigue efficacy is improved, the side effect of the composition on the liver is further reduced, and the better effects of protecting the liver and resisting fatigue are achieved.