72 results about "Malonic Aldehyde" patented technology

The invention provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of medicinal preparations. The preparation method comprises the following steps: (1) crushing; (2) extracting; (3) standing and separating; and (4) freezing and filtering to obtain the medicinal liquor. The medicinal liquor is obtained by extracting the traditional Chinese medicine components through the steps of crushing in batches, mixing and extracting, standing, freezing and separating the supernate and the suspension in different separation modes, and the medicinal liquor can better improve the space exploration capability of a mouse aged due to D-galactose, can reduce malondialdehyde MDA, and can improve the immunity of the mouse. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) is improved, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention discloses a method for efficiently screening saline-alkaline tolerant rice. According to the method, controllable indoor saline-alkali environment simulation conditions are constructed, various salts are mixed in proportion to prepare solutions with different salinity gradients, the pH value is adjusted, and temperature, humidity and illumination parameters are accurately controlled; the method comprises the following steps: disinfecting rice seeds, carrying out a germination test, transplanting rice seedlings, and regularly measuring growth indexes; in the treatment process, physiological indexes such as malondialdehyde content, antioxidant enzyme activity, proline content and the like and the expression level of salt and alkali tolerance related genes are detected, key indexes are screened by applying statistics and multivariate analysis methods, and an evaluation system is established for comprehensive score screening. The method can quickly perform saline-alkali stress treatment on a large number of rice seeds or seedlings, is not limited by natural environment, shortens the screening period, can accurately simulate different saline-alkali soil environments, scientifically evaluates the saline-alkali tolerance of rice from multiple dimensions, and provides an efficient and reliable technical means for breeding saline-alkali tolerant rice varieties.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention discloses a polypeptide fertilizer beneficial to cold resistance of crops and a preparation method thereof, and belongs to the technical field of agricultural fertilizers, the fertilizer is prepared by taking livestock and poultry blood (preferably pig blood) as a raw material, preparing high iron protein peptide through a compound protease hydrolysis process, and matching with a trace element compound, humic acid and a carrier. The preparation process comprises the steps of enzymolysis preparation of the ferritin peptide, fertilizer mixing, granulation, drying and the like. The polypeptide fertilizer disclosed by the invention takes the high-iron protein peptide as a core, the contained small-molecular amino acid polypeptide can enhance the antioxidant enzyme activity of crops at low temperature and reduce malonaldehyde accumulation, the porphyrin iron can promote chlorophyll synthesis and energy metabolism, and the high-iron protein peptide and the porphyrin iron cooperate to remarkably improve the cold resistance of the crops. Meanwhile, by cooperating with trace elements and humic acid, the cold resistance is enhanced, nutrient absorption of crops is promoted, the yield and quality are improved, multiple effects of resisting cold, improving quality, increasing yield and the like are achieved, the raw materials are wide in source, the cost is low, the preparation process is simple, and large-scale production and application are facilitated.

The invention belongs to the technical field of preparation of peptides, and particularly relates to camel placenta hydrolysate as well as a preparation method and application thereof. According to the preparation method of the camel placenta hydrolysate and the preparation method of the camel placenta hydrolysate provided by the invention, the camel placenta hydrolysate has remarkable scavenging activity on DPPH, ABTS, hydroxyl free radicals and iron ions, so that the antioxidant activity is exerted; in a fruit fly acute oxidative damage model, the camel placenta hydrolysate can prolong the survival time of fruit flies in an oxidative stress environment; in a normal fruit fly model, the camel placenta hydrolysate can prolong the life of normal aging fruit flies, improve the action ability of the aging fruit flies and play an anti-aging role by improving the activity of in-vivo antioxidant enzymes such as total superoxide dismutase, copper-zinc superoxide dismutase and catalase and reducing the malondialdehyde level on the premise of not reducing the food intake of the fruit flies. The camel placenta hydrolysate can be used as an ideal auxiliary antioxidant and anti-aging medicine and health food raw material.

The invention discloses an application of a pyrrosia lingua aqueous extract in preparation of a medicine for preventing and treating radioactive intestinal injury. Radioactive intestinal injury is a common complication of ionizing radiation, and has main pathological characteristics of intestinal mucosal barrier destruction, excessive release of inflammatory factors, increase of oxidative stress level and hematopoiesis and immune function inhibition. At present, no effective and safe prevention and treatment medicine is available. It is found for the first time that the pyrrosia lingua aqueous extract can inhibit expression (IL-6, TNF-alpha and IL-1beta) of inflammatory factors of radiation-induced MODE-K cells and relieve the oxidative stress level (malondialdehyde (MDA) is reduced, and the content of superoxide dismutase (SOD) and glutathione (GSH) is increased). In animal experiments, the pyrrosia lingua aqueous extract can reduce the levels of TNF-alpha and IL-1beta inflammatory factors in serum of irradiated mice and improve oxidative stress disorders such as MDA increase, SOD and GSH decrease and the like; the reduction of leukocytes and lymphocytes is relieved; the length of intestinal villus is promoted and recovered; the proportion of Ki67 positive cells in a crypt region is increased, so that the regeneration capacity of intestinal epithelial cells is enhanced. The pyrrosia lingua aqueous extract disclosed by the invention provides an experimental basis and an application prospect for developing a novel medicine for preventing and treating the radioactive intestinal injury.

The invention relates to the technical field of microorganisms, and discloses a talaromyces funiculi LD-YGH31 and an application of the talaromyces funiculi LD-YGH31 in salt tolerance. The strain is separated from the root system of halophyte, and the preservation number of the strain is CCTCC NO: M 20251815. After the strain is cultured, the strain and plants are co-cultured, and the growth performance and stress resistance of the plants under salt stress can be remarkably improved. Experiments prove that when the strain and tomato seedlings are co-cultured, under the stress condition of 200 mM NaCl salt, the fresh weight and plant height of the tomato seedlings can be remarkably improved, the activity of superoxide dismutase and peroxidase is improved, the content of malondialdehyde and the relative conductivity are reduced, and the K < + > / Na < + > steady state in plant roots and leaves is maintained. The strain is novel, the application method is simple and easy to implement, and the strain can be developed into a microbial fertilizer for saline-alkali soil crop planting and has remarkable agricultural application value.

The invention belongs to the technical field of microbial agents, and particularly relates to a composite microbial agent, a composite liquid microbial agent and application. The invention provides a complex microbial inoculant, which is prepared from bacillus mojavensis W1, bacillus halotolerans L21 and bacillus subtilis M4, wherein the complex microbial inoculant is prepared from bacillus mojavensis, bacillus halotolerans L21 and bacillus subtilis M4, and the complex microbial inoculant is prepared from bacillus mojavensis, bacillus halotolerans L21 and bacillus subtilis M4. The bacillus mojavensis W1, the bacillus halotolerant L21 and the bacillus subtilis M4 in the complex microbial inoculant interact with one another, so that the defense mechanism of plants can be activated, the activity of superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT) can be improved, and the content of malondialdehyde (MDA) can be reduced, thereby improving the prevention and treatment effect on the root rot of the cornutgionium axentgii.

The invention belongs to the technical field of microbial control, and particularly relates to a salt-tolerant bacillus, a microbial agent, a preparation method, a control method and application. The invention provides a salt-tolerant bacillus L21 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35478. The salt-tolerant bacillus L21 can activate the defense mechanism of plants, improve the activity of defensive enzymes in the plants, and enable the plants to have higher oxidation resistance when encountering pathogenic bacteria, so that ROS accumulation is more effectively reduced, MDA is reduced, and the morbidity of root rot is reduced. The result of the embodiment shows that the bacillus halotolerant L21 can improve the activity of superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT), reduce the content of malondialdehyde (MDA) and reduce the morbidity of root rot of cornutgionium axataenense.

The application discloses application of Ligusticum sinense alcohol extract in preparation of a medicine for preventing or treating drug-induced liver injury, and relates to the technical field of biological medicine.The application proves for the first time that the Ligusticum sinense alcohol extract has an obvious effect of improving acute drug-induced liver injury, can obviously improve serum biochemical indexes, inflammatory factor levels, and liver tissue pathological states of mice with acute drug-induced liver injury, and improve superoxide dismutase (SOD) and glutathione (GSH) activities in liver tissue and reduce malondialdehyde (MDA) and reactive oxygen species (ROS) levels.The pharmacodynamics test proves that the Ligusticum sinense alcohol extract has a significant liver injury protection effect, and has a low effective dose and no toxic side effect.Therefore, the application can become an alternative medicine for preventing and treating drug-induced liver injury.

The invention belongs to the technical field of microbial prevention and control, and particularly relates to bacillus subtilis, a microbial agent, a preparation method, a prevention and control method and application. The invention provides a strain of bacillus subtilis M4, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35477. The bacillus subtilis M4 is used for preventing and treating root rot of cornutgionium axe. The bacillus subtilis M4 can improve the activity of disease-resistant defensin superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT), reduce the content of malondialdehyde (MDA), finally reduce the morbidity and the morbidity index of plant root rot and promote plant growth.

This invention relates to the field of bioengineering technology and provides an application of the maize heat shock transcription factor gene ZmHSF18, wherein the nucleotide sequence of the maize heat shock transcription factor gene ZmHSF18 is shown in SEQ ID NO:1. Experiments have demonstrated that overexpression of the maize heat shock transcription factor gene ZmHSF18 in plants can significantly improve seed germination rate and frozen survival rate at the mature stage under low-temperature stress, reduce malondialdehyde accumulation, and increase superoxide dismutase activity. Therefore, this maize heat shock transcription factor gene ZmHSF18 can be used to improve plant cold tolerance and create transgenic plants with enhanced cold tolerance, possessing important application value in plant genetic engineering and stress resistance breeding.

This invention discloses the application of burdock root polysaccharide in the preparation of drugs for treating cognitive impairment, belonging to the field of traditional Chinese medicine technology. Experiments demonstrate that burdock root polysaccharide can improve cognitive impairment and independent exploration ability in D-galactose-induced cognitive impairment model mice, increase superoxide dismutase activity in mouse serum, reduce malondialdehyde content, decrease the content of interleukin-1β and tumor necrosis factor-α in the hippocampus of mouse brains, and alleviate the loss of neurons in the hippocampus of cognitive impairment model mice, thereby achieving a neuroprotective effect and demonstrating a good therapeutic effect on cognitive impairment. This provides a new approach for developing drugs related to the treatment of cognitive impairment.

The invention discloses an application of a flammulina velutipes extract in preparation of related products for relieving and / or treating drug-induced liver injury. By constructing a drug-induced liver injury model, it is found that by means of the needle mushroom extract rich in needle mushroom polysaccharide in low dosage, liver pathological injuries such as cisplatin-induced hepatocyte swelling and necrosis can be remarkably relieved, the levels of serum liver function indexes of alanine transaminase and aspartate transaminase are reduced, and the liver injury resistance is improved. And meanwhile, the activity of superoxide dismutase and glutathione peroxidase in liver tissues is improved, the concentration of malonaldehyde is reduced, and excellent antioxidant characteristics are shown. In addition, the invention also discovers that the flammulina velutipes extract can improve the composition of intestinal microorganisms, promote colonization of beneficial bacteria, reduce the abundance of harmful bacteria and regulate the key metabolic pathway of the liver at the same time, and can provide a treatment strategy for the treatment of drug-induced liver injury.

The invention relates to the technical field of plant stress resistance regulation and control and the field of plant genetic engineering, in particular to application of strigolactones GR24 or induced protein CsJIP-like thereof in improving cold resistance of tea trees, a strigolactones GR24 solution is sprayed to the tea trees, the relative conductivity of the tea trees and the content of malondialdehyde, hydrogen peroxide and superoxide anions can be reduced, and the cold resistance of the tea trees can be improved. The activity of superoxide dismutase, peroxidase, catalase and ascorbic acid peroxidase in the tea trees is improved, the oxidative stress of the tea trees is relieved, the integrity of cell membranes is protected, cell osmotic equilibrium is maintained, and dehydration damage caused by low temperature is avoided; screening a cold-resistant gene CsJIP-like responding to GR24, and verifying that the cold-resistant gene CsJIP-like has a cold-resistant function by utilizing instantaneous overexpression and instantaneous silencing of tea tree leaves; the GR24 is high in chemical stability, small in application amount, environmentally friendly and beneficial to promoting the genetic improvement process of cold resistance of the tea trees.

The invention discloses calf animal coccus PD13 for improving Alzheimer's disease and application of the calf animal coccus PD13, and relates to the technical field of biology. Experiments prove that in an Alzheimer's disease model mouse induced by injecting A beta oligomer into the brain, the Azococcus calvus PD13 can improve spatial learning and memory disorder and autonomous exploration ability of the AD model mouse and relieve damage to neurons in a hippocampal region of the Alzheimer's disease model mouse, so that the effect of protecting the neurons is achieved, and the Azococcus calvus PD13 can be used for preparing a medicine for treating the Alzheimer's disease. According to the present invention, with the application of the animal coccus calves, the content of glutathione in mouse serum and the enzyme activity of superoxide dismutase are increased, and the content of malondialdehyde is reduced, such that the animal coccus calves provides good prevention and treatment effects on the Alzheimer's disease, and the new approach is provided for developing the Alzheimer's disease-related drugs.

The invention discloses an application of ligularia virgaurea alcohol extract in preparation of a medicine for preventing or treating drug-induced liver injury, relates to the technical field of biological medicines, and proves that the ligularia virgaurea alcohol extract has an obvious effect of improving acute drug-induced liver injury for the first time. The traditional Chinese medicine composition can obviously improve serum biochemical indexes, inflammatory factor levels and hepatic tissue pathological states of mice with acute drug-induced liver injury, improve the activity of superoxide dismutase (SOD) and glutathione (GSH) in hepatic tissues, and reduce the levels of malondialdehyde (MDA) and reactive oxygen species (ROS). Pharmacodynamic tests prove that the ligularia nobilis alcohol extract has a remarkable liver injury protection effect, and is low in effective dose and free of toxic and side effects. Therefore, the invention may become an alternative drug for preventing and treating drug-induced liver injury.

The present invention provides an application of chitosan oligosaccharide aminomethylthiobutyramide derivatives in plant resistance to salt stress, belonging to the field of agricultural biotechnology. The derivatives are used to alleviate the oxidative damage of plants caused by salt stress, specifically including the following effects: (1) reducing the malondialdehyde content in plants; (2) increasing the activities of superoxide dismutase and peroxidase; and (3) promoting seed germination and seedling growth. The derivatives are formed by connecting the carboxyl group of methionine with the amino group of chitosan oligosaccharide through an amide bond, and the derivatives are 2-amino-4-methylthiobutyryl chitosan oligosaccharide, which is a methionine high-molecular chitosan oligosaccharide with a deacetylation degree of ≥90%. The polymerization degree of the methionine high-molecular chitosan oligosaccharide is 10-18. The present invention provides a methionine carrier for loading methionine into plants to improve the effect of plant resistance to salt stress.

The invention relates to application of germplasm of botryosphaeria formosa to preparation of health-care products for improving antioxidation, and belongs to the technical field of functional evaluation of health-care foods, the germplasm of botryosphaeria formosa promotes expression of superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-PX) by inhibiting accumulation of peroxide malonaldehyde (MDA) in heart, liver and serum, so that the anti-oxidation effect of the botryosphaeria formosa is improved, and the anti-oxidation effect of the botryosphaeria formosa is improved. The anti-oxidation regulation function is realized. The boil bean germplasm is extracted for 30 minutes by adopting boiling water, so that the use of an organic solvent is avoided to retain natural active ingredients, and the boil bean milk containing 1g / mL of crude drug is obtained and is used for preparing antioxidant health-care products. The health-care product is suitable for prevention or adjuvant treatment of diseases caused by oxidative stress, such as cardiovascular diseases, liver injury or aging related diseases. Experiments prove that the anti-oxidation effect of the germplasm of the botryosphaeria formosa is evaluated, the germplasm of the botryosphaeria formosa has a reliable anti-oxidation regulation effect, the MDA level in the heart, the liver and serum can be remarkably reduced, and the expression of SOD, GSH and GSH-PX is promoted.

The present application provides a composition with satiety and weight control and anti-aging, and a preparation method and application thereof. The composition comprises the following raw materials by weight: polysaccharide 11-13 parts, fiber powder 20-25 parts, highland barley powder 12-18 parts, compound powder 18-23 parts, pectin 4-8 parts, ganoderma lucidum powder 6-9 parts, and compound protein 8-12 parts, and relates to the technical field of satiety and weight control and anti-aging compositions. The composition can form an effect of hindering nutrient absorption or prolonging gastric emptying, increase the volume of intestinal contents to achieve satiety, thereby achieving the functions of well controlling weight gain and reducing body weight. In addition, the composition can activate the body's antioxidant enzyme system, improve the activity of superoxide dismutase (SOD), and reduce the content of malondialdehyde (MDA), thereby delaying cell aging and achieving the anti-aging effect.

The application discloses a kind of synthetic functional microbial inoculants and its application in salt-tolerant growth promotion of wheat, adopt hydrophobic gordon's bacteria ( Gordonia hydrophobica ), odyssey lysine bacillus ( Lysinibacillus odysseyi ), sea bed motility microorganism ( Planomicrobium okeanokoites ), north China broussonetia yellow color bacterium ( Luteimonas huabeiensis ), epidermis brevibacterium ( Brevibacterium epidermidis ) five strains of bacteria, microbial inoculant has no antagonism between strains, salt-tolerant, and other significant characteristics such as viable count, can significantly promote spring wheat germination rate, seedling length and root length, and has significant promoting effect on seedling length and root length of winter wheat.Under salt stress conditions, the microbial inoculant can significantly improve the activities of catalase, peroxidase and superoxide dismutase of wheat, and the total chlorophyll content;after treatment with the microbial inoculant, the contents of malondialdehyde and proline of wheat are significantly reduced.The microbial inoculant plays a promoting and protecting role in the process of resisting salt stress of spring wheat and winter wheat, and has an impact on the development and sustainable development of arid region agriculture.

The invention discloses a lemon fresh-keeping method based on calcium-salicylic acid and tea polyphenol-chitosan. The lemon fresh-keeping method comprises the steps of pretreatment before picking, soaking treatment after picking and storage management. 14 days before picking, a compound solution of 0.23 g / L chelated calcium and 2.0 mmol / L salicylic acid is sprayed to lemon fruits; after harvesting, the fruits are soaked in a 2% tea polyphenol and 1% chitosan composite fresh-keeping solution for 3 min, and the fruits are stored at the normal temperature of 25 DEG C after being dried in the shade; through the synergistic treatment, the hardness increase, weight loss rate increase and color deterioration of the lemon fruits can be obviously delayed, relatively high contents of total acids, total phenols, ascorbic acid and flavonoids are maintained, the accumulation of malonaldehyde is inhibited, and the activity of superoxide dismutase, peroxidase and catalase is improved; after the fruits are stored for 40 days, the weight loss rate of the fruits is only 30.37%, the total phenol content is 0.486 mg / mL, the SOD activity is 46.63 U / mL, and the fresh-keeping effect is obviously superior to that of a single treatment group and a control group; the method is simple in process, controllable in cost and high in safety.

The application provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: (1) crushing; (2) extraction; (3) standing and separation; (4) freezing and filtration, so that the medicinal liquor is obtained. The medicinal liquor is obtained by adopting the steps of batch crushing, mixed extraction, standing, freezing and separating supernatant and suspension by using different separation methods to extract traditional Chinese medicine ingredients. The medicinal liquor can better improve the space exploration ability of aging mice caused by D-galactose, reduce malondialdehyde (MDA), improve superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and has significant antioxidant and anti-aging effects.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to application of a related biological product of an StIQD20 gene to improvement of plant salt tolerance. The nucleotide sequence of the StIQD20 gene is shown as SEQ ID NO.1, the StIQD20 gene can regulate and control the influence of salt stress on the growth and development of potato seedlings, and a potato strain which is over-expressed with the StIQD20 gene and is cultivated by using related biological products of the StIQD20 gene shows remarkable agronomic advantages under the salt stress: under the treatment condition of 100 mmol / L NaCl, the salt stress of the potato strain is reduced, and the salt stress of the potato strain is reduced. The plant height, the leaf number and the fresh weight of a potato plant over-expressed with the StIQD20 gene are 1.2-3.8 times of those of a wild type, the activity of superoxide dismutase and peroxidase is improved by 1.25-3.3 times, and the malondialdehyde content is reduced by 10-70%. By using related biological products of the StIQD20 gene, the anti-oxidation defense capability of plants to salt stress can be improved, and cell damage is reduced.

The invention provides application of an OsAAG8b gene to improvement of drought resistance of rice, and belongs to the technical field of biological genes. The nucleotide sequence of the OsAAG8b gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the OsAAG8b gene is as shown in SEQ ID NO: 2. Compared with a wild type (WT), the survival rate of the OsAAG8b gene overexpressed plant OsAAG8b-OE under the PEG-induced drought stress is obviously higher than that of the WT. Compared with WT, the OsAAG8b overexpression plant after PEG induction of drought stress has the advantages that the content of soluble sugar, proline, superoxide dismutase and catalase of the plant on the drought stress caused by PEG is increased, and the content of hydrogen peroxide and malondialdehyde is reduced. The OsAAG8b gene is a new candidate target for potentially researching a mechanism for regulating and controlling the drought resistance of the rice.

The invention discloses a sea-buckthorn compressed solid food with an anti-hypoxia function and a preparation method thereof, and is characterized in that a starch-sea-buckthorn compound is prepared by a pre-gelatinization and high-speed shearing method, then the compressed solid food is prepared, 100% of low-gluten flour, 5% of the starch-sea-buckthorn compound and 8% of whole milk powder are uniformly mixed by taking the mass dosage of the low-gluten flour as a reference, and then the low-gluten flour, the starch-sea-buckthorn compound and the whole milk powder are uniformly mixed to obtain the sea-buckthorn compressed solid food with the anti-hypoxia function. Adding 19% of palm oil, 6% of white granulated sugar, 1% of baking soda, 1% of table salt and 16% of water, and uniformly mixing and stirring to prepare dough; and baking the prepared dough in an oven, cooling to room temperature, crushing, mixing, pressing and packaging to obtain the sea-buckthorn compressed solid food. The compressed solid food can prolong the survival time of mice under normal-pressure closed hypoxia and sodium nitrite poisoning, increase the content of superoxide dismutase in livers of the mice and reduce the content of malondialdehyde in hearts, livers and lungs of the mice, so that the anti-hypoxia effect is achieved. The product is convenient to carry and store, and a new way can be provided for research and development of anti-hypoxia functional products.

The application discloses a kind of biological source pesticides for preventing and treating aigan's root rot, it includes one or more than two combinations of berberine, ningnanmycin, Sophora flavescens extract, zhongshengmycin, allicin, polyoxin, matrine, osthol, lentinan.The application takes aigan's root and rhizome infected with root rot as research object, obtains a strain of pathogenic bacteria from disease-healthy boundary by separation, purification and pathogenicity determination, and is identified as bean shell ball spores by morphological observation, rDNA-ITS sequence analysis Macrophomina phaseolina Comprehensive indoor and field screening results, the most effective pesticide screened includes 3% zhongshengmycin, 0.5% berberine, 3.5% Sophora flavescens extract and 8% ningnanmycin, and all does not show plant adverse effect and phytotoxicity to aigan growth.Especially, it can significantly reduce malondialdehyde level, while increasing the activity of superoxide dismutase, catalase and peroxidase, so as to improve the disease resistance of aigan.

The invention provides a corn peptide with a neuroprotective effect as well as a preparation method and application thereof. Wherein the functional peptide fragment comprises a peptide fragment GL, a peptide fragment FA and a peptide fragment FQ, and the functional peptide fragment has good biological safety; the nerve cell injury caused by A beta1-42 can be effectively inhibited, and the survival rate of injured cells is improved; the mitochondrial membrane potential intensity of nerve cells under A beta1-42 injury can be improved, so that the mitochondrial membrane potential dysfunction is improved; the cell membrane damage of nerve cells under Abeta1-42 damage can be inhibited, so that the release of lactic dehydrogenase is reduced; the active oxygen content and malondialdehyde content of nerve cells damaged by A beta1-42 can be reduced, and the superoxide dismutase content and glutathione peroxidase can be improved, so that the oxidation-antioxidation effect of the body is promoted to be maintained in a balanced state, the negative effects of oxidative stress and the like are avoided, and the nerve protection effect is further achieved.

The invention belongs to the technical field of traditional Chinese medicine planting, and particularly relates to application of strigolactone biosynthesis inhibitor in promoting gastrodia elata seed breeding. The strigolactones biosynthesis inhibitor has the function of specifically blocking SL synthesis key enzyme, and is used for treating gastrodia elata tubers, so that the content of superoxide anions in gastrodia elata and the activity of superoxide dismutase and catalase can be enhanced, the content of soluble solids and malondialdehyde in gastrodia elata and the activity of peroxidase are reduced, and the yield of gastrodia elata is increased. Therefore, the bud dormancy of the gastrodia elata is favorably broken, the germination of latent buds of the gastrodia elata is promoted, and efficient, simple and large-scale breeding of the gastrodia elata seeds is realized.

The invention discloses a mulberry heat shock transcription factor MaHSF1 as well as a coding gene and application thereof, and aims to solve the technical problem that high-temperature-resistant gene resources and germplasm resources of mulberry are deficient at present. On the basis of the heat shock transcription factor MaHSF1 which is screened from a mulberry HSF gene family and is obviously subjected to high-temperature stress up-regulation and heterologous expression of which can improve the high-temperature resistance of the yeast, the over-expression of the heat shock transcription factor MaHSF1 can inhibit the increase of the content of malondialdehyde MDA at high temperature; the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and the content of osmotic regulation substance free proline are improved, so that the high-temperature resistance of arabidopsis thaliana is improved; the expression of the promoter has developmental stage specificity and heat stress induction characteristics, which indicates the accuracy of the regulation mechanism of the promoter. According to the application, the function and the action mechanism of the MaHSF1 and the coding gene thereof for resisting the high-temperature stress damage are verified for the first time, and a solid theoretical basis is provided for the mulberry production to cope with the high-temperature stress damage and the breeding of new high-temperature-resistant varieties.