53 results about "Malonic Aldehyde" patented technology

The invention provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of medicinal preparations. The preparation method comprises the following steps: (1) crushing; (2) extracting; (3) standing and separating; and (4) freezing and filtering to obtain the medicinal liquor. The medicinal liquor is obtained by extracting the traditional Chinese medicine components through the steps of crushing in batches, mixing and extracting, standing, freezing and separating the supernate and the suspension in different separation modes, and the medicinal liquor can better improve the space exploration capability of a mouse aged due to D-galactose, can reduce malondialdehyde MDA, and can improve the immunity of the mouse. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) is improved, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention discloses a polypeptide fertilizer beneficial to cold resistance of crops and a preparation method thereof, and belongs to the technical field of agricultural fertilizers, the fertilizer is prepared by taking livestock and poultry blood (preferably pig blood) as a raw material, preparing high iron protein peptide through a compound protease hydrolysis process, and matching with a trace element compound, humic acid and a carrier. The preparation process comprises the steps of enzymolysis preparation of the ferritin peptide, fertilizer mixing, granulation, drying and the like. The polypeptide fertilizer disclosed by the invention takes the high-iron protein peptide as a core, the contained small-molecular amino acid polypeptide can enhance the antioxidant enzyme activity of crops at low temperature and reduce malonaldehyde accumulation, the porphyrin iron can promote chlorophyll synthesis and energy metabolism, and the high-iron protein peptide and the porphyrin iron cooperate to remarkably improve the cold resistance of the crops. Meanwhile, by cooperating with trace elements and humic acid, the cold resistance is enhanced, nutrient absorption of crops is promoted, the yield and quality are improved, multiple effects of resisting cold, improving quality, increasing yield and the like are achieved, the raw materials are wide in source, the cost is low, the preparation process is simple, and large-scale production and application are facilitated.

The invention discloses an application of a pyrrosia lingua aqueous extract in preparation of a medicine for preventing and treating radioactive intestinal injury. Radioactive intestinal injury is a common complication of ionizing radiation, and has main pathological characteristics of intestinal mucosal barrier destruction, excessive release of inflammatory factors, increase of oxidative stress level and hematopoiesis and immune function inhibition. At present, no effective and safe prevention and treatment medicine is available. It is found for the first time that the pyrrosia lingua aqueous extract can inhibit expression (IL-6, TNF-alpha and IL-1beta) of inflammatory factors of radiation-induced MODE-K cells and relieve the oxidative stress level (malondialdehyde (MDA) is reduced, and the content of superoxide dismutase (SOD) and glutathione (GSH) is increased). In animal experiments, the pyrrosia lingua aqueous extract can reduce the levels of TNF-alpha and IL-1beta inflammatory factors in serum of irradiated mice and improve oxidative stress disorders such as MDA increase, SOD and GSH decrease and the like; the reduction of leukocytes and lymphocytes is relieved; the length of intestinal villus is promoted and recovered; the proportion of Ki67 positive cells in a crypt region is increased, so that the regeneration capacity of intestinal epithelial cells is enhanced. The pyrrosia lingua aqueous extract disclosed by the invention provides an experimental basis and an application prospect for developing a novel medicine for preventing and treating the radioactive intestinal injury.

The invention relates to the technical field of microorganisms, and discloses a talaromyces funiculi LD-YGH31 and an application of the talaromyces funiculi LD-YGH31 in salt tolerance. The strain is separated from the root system of halophyte, and the preservation number of the strain is CCTCC NO: M 20251815. After the strain is cultured, the strain and plants are co-cultured, and the growth performance and stress resistance of the plants under salt stress can be remarkably improved. Experiments prove that when the strain and tomato seedlings are co-cultured, under the stress condition of 200 mM NaCl salt, the fresh weight and plant height of the tomato seedlings can be remarkably improved, the activity of superoxide dismutase and peroxidase is improved, the content of malondialdehyde and the relative conductivity are reduced, and the K < + > / Na < + > steady state in plant roots and leaves is maintained. The strain is novel, the application method is simple and easy to implement, and the strain can be developed into a microbial fertilizer for saline-alkali soil crop planting and has remarkable agricultural application value.

The invention belongs to the technical field of microbial agents, and particularly relates to a composite microbial agent, a composite liquid microbial agent and application. The invention provides a complex microbial inoculant, which is prepared from bacillus mojavensis W1, bacillus halotolerans L21 and bacillus subtilis M4, wherein the complex microbial inoculant is prepared from bacillus mojavensis, bacillus halotolerans L21 and bacillus subtilis M4, and the complex microbial inoculant is prepared from bacillus mojavensis, bacillus halotolerans L21 and bacillus subtilis M4. The bacillus mojavensis W1, the bacillus halotolerant L21 and the bacillus subtilis M4 in the complex microbial inoculant interact with one another, so that the defense mechanism of plants can be activated, the activity of superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT) can be improved, and the content of malondialdehyde (MDA) can be reduced, thereby improving the prevention and treatment effect on the root rot of the cornutgionium axentgii.

The invention belongs to the technical field of microbial control, and particularly relates to a salt-tolerant bacillus, a microbial agent, a preparation method, a control method and application. The invention provides a salt-tolerant bacillus L21 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35478. The salt-tolerant bacillus L21 can activate the defense mechanism of plants, improve the activity of defensive enzymes in the plants, and enable the plants to have higher oxidation resistance when encountering pathogenic bacteria, so that ROS accumulation is more effectively reduced, MDA is reduced, and the morbidity of root rot is reduced. The result of the embodiment shows that the bacillus halotolerant L21 can improve the activity of superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT), reduce the content of malondialdehyde (MDA) and reduce the morbidity of root rot of cornutgionium axataenense.

The application discloses application of Ligusticum sinense alcohol extract in preparation of a medicine for preventing or treating drug-induced liver injury, and relates to the technical field of biological medicine.The application proves for the first time that the Ligusticum sinense alcohol extract has an obvious effect of improving acute drug-induced liver injury, can obviously improve serum biochemical indexes, inflammatory factor levels, and liver tissue pathological states of mice with acute drug-induced liver injury, and improve superoxide dismutase (SOD) and glutathione (GSH) activities in liver tissue and reduce malondialdehyde (MDA) and reactive oxygen species (ROS) levels.The pharmacodynamics test proves that the Ligusticum sinense alcohol extract has a significant liver injury protection effect, and has a low effective dose and no toxic side effect.Therefore, the application can become an alternative medicine for preventing and treating drug-induced liver injury.

The invention belongs to the technical field of microbial prevention and control, and particularly relates to bacillus subtilis, a microbial agent, a preparation method, a prevention and control method and application. The invention provides a strain of bacillus subtilis M4, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35477. The bacillus subtilis M4 is used for preventing and treating root rot of cornutgionium axe. The bacillus subtilis M4 can improve the activity of disease-resistant defensin superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT), reduce the content of malondialdehyde (MDA), finally reduce the morbidity and the morbidity index of plant root rot and promote plant growth.

This invention relates to the field of bioengineering technology and provides an application of the maize heat shock transcription factor gene ZmHSF18, wherein the nucleotide sequence of the maize heat shock transcription factor gene ZmHSF18 is shown in SEQ ID NO:1. Experiments have demonstrated that overexpression of the maize heat shock transcription factor gene ZmHSF18 in plants can significantly improve seed germination rate and frozen survival rate at the mature stage under low-temperature stress, reduce malondialdehyde accumulation, and increase superoxide dismutase activity. Therefore, this maize heat shock transcription factor gene ZmHSF18 can be used to improve plant cold tolerance and create transgenic plants with enhanced cold tolerance, possessing important application value in plant genetic engineering and stress resistance breeding.

This invention discloses the application of burdock root polysaccharide in the preparation of drugs for treating cognitive impairment, belonging to the field of traditional Chinese medicine technology. Experiments demonstrate that burdock root polysaccharide can improve cognitive impairment and independent exploration ability in D-galactose-induced cognitive impairment model mice, increase superoxide dismutase activity in mouse serum, reduce malondialdehyde content, decrease the content of interleukin-1β and tumor necrosis factor-α in the hippocampus of mouse brains, and alleviate the loss of neurons in the hippocampus of cognitive impairment model mice, thereby achieving a neuroprotective effect and demonstrating a good therapeutic effect on cognitive impairment. This provides a new approach for developing drugs related to the treatment of cognitive impairment.

The invention relates to application of germplasm of botryosphaeria formosa to preparation of health-care products for improving antioxidation, and belongs to the technical field of functional evaluation of health-care foods, the germplasm of botryosphaeria formosa promotes expression of superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-PX) by inhibiting accumulation of peroxide malonaldehyde (MDA) in heart, liver and serum, so that the anti-oxidation effect of the botryosphaeria formosa is improved, and the anti-oxidation effect of the botryosphaeria formosa is improved. The anti-oxidation regulation function is realized. The boil bean germplasm is extracted for 30 minutes by adopting boiling water, so that the use of an organic solvent is avoided to retain natural active ingredients, and the boil bean milk containing 1g / mL of crude drug is obtained and is used for preparing antioxidant health-care products. The health-care product is suitable for prevention or adjuvant treatment of diseases caused by oxidative stress, such as cardiovascular diseases, liver injury or aging related diseases. Experiments prove that the anti-oxidation effect of the germplasm of the botryosphaeria formosa is evaluated, the germplasm of the botryosphaeria formosa has a reliable anti-oxidation regulation effect, the MDA level in the heart, the liver and serum can be remarkably reduced, and the expression of SOD, GSH and GSH-PX is promoted.

The present application provides a composition with satiety and weight control and anti-aging, and a preparation method and application thereof. The composition comprises the following raw materials by weight: polysaccharide 11-13 parts, fiber powder 20-25 parts, highland barley powder 12-18 parts, compound powder 18-23 parts, pectin 4-8 parts, ganoderma lucidum powder 6-9 parts, and compound protein 8-12 parts, and relates to the technical field of satiety and weight control and anti-aging compositions. The composition can form an effect of hindering nutrient absorption or prolonging gastric emptying, increase the volume of intestinal contents to achieve satiety, thereby achieving the functions of well controlling weight gain and reducing body weight. In addition, the composition can activate the body's antioxidant enzyme system, improve the activity of superoxide dismutase (SOD), and reduce the content of malondialdehyde (MDA), thereby delaying cell aging and achieving the anti-aging effect.

The application discloses a kind of synthetic functional microbial inoculants and its application in salt-tolerant growth promotion of wheat, adopt hydrophobic gordon's bacteria ( Gordonia hydrophobica ), odyssey lysine bacillus ( Lysinibacillus odysseyi ), sea bed motility microorganism ( Planomicrobium okeanokoites ), north China broussonetia yellow color bacterium ( Luteimonas huabeiensis ), epidermis brevibacterium ( Brevibacterium epidermidis ) five strains of bacteria, microbial inoculant has no antagonism between strains, salt-tolerant, and other significant characteristics such as viable count, can significantly promote spring wheat germination rate, seedling length and root length, and has significant promoting effect on seedling length and root length of winter wheat.Under salt stress conditions, the microbial inoculant can significantly improve the activities of catalase, peroxidase and superoxide dismutase of wheat, and the total chlorophyll content;after treatment with the microbial inoculant, the contents of malondialdehyde and proline of wheat are significantly reduced.The microbial inoculant plays a promoting and protecting role in the process of resisting salt stress of spring wheat and winter wheat, and has an impact on the development and sustainable development of arid region agriculture.

The invention discloses a lemon fresh-keeping method based on calcium-salicylic acid and tea polyphenol-chitosan. The lemon fresh-keeping method comprises the steps of pretreatment before picking, soaking treatment after picking and storage management. 14 days before picking, a compound solution of 0.23 g / L chelated calcium and 2.0 mmol / L salicylic acid is sprayed to lemon fruits; after harvesting, the fruits are soaked in a 2% tea polyphenol and 1% chitosan composite fresh-keeping solution for 3 min, and the fruits are stored at the normal temperature of 25 DEG C after being dried in the shade; through the synergistic treatment, the hardness increase, weight loss rate increase and color deterioration of the lemon fruits can be obviously delayed, relatively high contents of total acids, total phenols, ascorbic acid and flavonoids are maintained, the accumulation of malonaldehyde is inhibited, and the activity of superoxide dismutase, peroxidase and catalase is improved; after the fruits are stored for 40 days, the weight loss rate of the fruits is only 30.37%, the total phenol content is 0.486 mg / mL, the SOD activity is 46.63 U / mL, and the fresh-keeping effect is obviously superior to that of a single treatment group and a control group; the method is simple in process, controllable in cost and high in safety.

The application provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: (1) crushing; (2) extraction; (3) standing and separation; (4) freezing and filtration, so that the medicinal liquor is obtained. The medicinal liquor is obtained by adopting the steps of batch crushing, mixed extraction, standing, freezing and separating supernatant and suspension by using different separation methods to extract traditional Chinese medicine ingredients. The medicinal liquor can better improve the space exploration ability of aging mice caused by D-galactose, reduce malondialdehyde (MDA), improve superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and has significant antioxidant and anti-aging effects.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to application of a related biological product of an StIQD20 gene to improvement of plant salt tolerance. The nucleotide sequence of the StIQD20 gene is shown as SEQ ID NO.1, the StIQD20 gene can regulate and control the influence of salt stress on the growth and development of potato seedlings, and a potato strain which is over-expressed with the StIQD20 gene and is cultivated by using related biological products of the StIQD20 gene shows remarkable agronomic advantages under the salt stress: under the treatment condition of 100 mmol / L NaCl, the salt stress of the potato strain is reduced, and the salt stress of the potato strain is reduced. The plant height, the leaf number and the fresh weight of a potato plant over-expressed with the StIQD20 gene are 1.2-3.8 times of those of a wild type, the activity of superoxide dismutase and peroxidase is improved by 1.25-3.3 times, and the malondialdehyde content is reduced by 10-70%. By using related biological products of the StIQD20 gene, the anti-oxidation defense capability of plants to salt stress can be improved, and cell damage is reduced.

The invention provides application of an OsAAG8b gene to improvement of drought resistance of rice, and belongs to the technical field of biological genes. The nucleotide sequence of the OsAAG8b gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the OsAAG8b gene is as shown in SEQ ID NO: 2. Compared with a wild type (WT), the survival rate of the OsAAG8b gene overexpressed plant OsAAG8b-OE under the PEG-induced drought stress is obviously higher than that of the WT. Compared with WT, the OsAAG8b overexpression plant after PEG induction of drought stress has the advantages that the content of soluble sugar, proline, superoxide dismutase and catalase of the plant on the drought stress caused by PEG is increased, and the content of hydrogen peroxide and malondialdehyde is reduced. The OsAAG8b gene is a new candidate target for potentially researching a mechanism for regulating and controlling the drought resistance of the rice.

The invention discloses a sea-buckthorn compressed solid food with an anti-hypoxia function and a preparation method thereof, and is characterized in that a starch-sea-buckthorn compound is prepared by a pre-gelatinization and high-speed shearing method, then the compressed solid food is prepared, 100% of low-gluten flour, 5% of the starch-sea-buckthorn compound and 8% of whole milk powder are uniformly mixed by taking the mass dosage of the low-gluten flour as a reference, and then the low-gluten flour, the starch-sea-buckthorn compound and the whole milk powder are uniformly mixed to obtain the sea-buckthorn compressed solid food with the anti-hypoxia function. Adding 19% of palm oil, 6% of white granulated sugar, 1% of baking soda, 1% of table salt and 16% of water, and uniformly mixing and stirring to prepare dough; and baking the prepared dough in an oven, cooling to room temperature, crushing, mixing, pressing and packaging to obtain the sea-buckthorn compressed solid food. The compressed solid food can prolong the survival time of mice under normal-pressure closed hypoxia and sodium nitrite poisoning, increase the content of superoxide dismutase in livers of the mice and reduce the content of malondialdehyde in hearts, livers and lungs of the mice, so that the anti-hypoxia effect is achieved. The product is convenient to carry and store, and a new way can be provided for research and development of anti-hypoxia functional products.

The application discloses a kind of biological source pesticides for preventing and treating aigan's root rot, it includes one or more than two combinations of berberine, ningnanmycin, Sophora flavescens extract, zhongshengmycin, allicin, polyoxin, matrine, osthol, lentinan.The application takes aigan's root and rhizome infected with root rot as research object, obtains a strain of pathogenic bacteria from disease-healthy boundary by separation, purification and pathogenicity determination, and is identified as bean shell ball spores by morphological observation, rDNA-ITS sequence analysis Macrophomina phaseolina Comprehensive indoor and field screening results, the most effective pesticide screened includes 3% zhongshengmycin, 0.5% berberine, 3.5% Sophora flavescens extract and 8% ningnanmycin, and all does not show plant adverse effect and phytotoxicity to aigan growth.Especially, it can significantly reduce malondialdehyde level, while increasing the activity of superoxide dismutase, catalase and peroxidase, so as to improve the disease resistance of aigan.

The invention belongs to the technical field of traditional Chinese medicine planting, and particularly relates to application of strigolactone biosynthesis inhibitor in promoting gastrodia elata seed breeding. The strigolactones biosynthesis inhibitor has the function of specifically blocking SL synthesis key enzyme, and is used for treating gastrodia elata tubers, so that the content of superoxide anions in gastrodia elata and the activity of superoxide dismutase and catalase can be enhanced, the content of soluble solids and malondialdehyde in gastrodia elata and the activity of peroxidase are reduced, and the yield of gastrodia elata is increased. Therefore, the bud dormancy of the gastrodia elata is favorably broken, the germination of latent buds of the gastrodia elata is promoted, and efficient, simple and large-scale breeding of the gastrodia elata seeds is realized.

The invention discloses a mulberry heat shock transcription factor MaHSF1 as well as a coding gene and application thereof, and aims to solve the technical problem that high-temperature-resistant gene resources and germplasm resources of mulberry are deficient at present. On the basis of the heat shock transcription factor MaHSF1 which is screened from a mulberry HSF gene family and is obviously subjected to high-temperature stress up-regulation and heterologous expression of which can improve the high-temperature resistance of the yeast, the over-expression of the heat shock transcription factor MaHSF1 can inhibit the increase of the content of malondialdehyde MDA at high temperature; the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and the content of osmotic regulation substance free proline are improved, so that the high-temperature resistance of arabidopsis thaliana is improved; the expression of the promoter has developmental stage specificity and heat stress induction characteristics, which indicates the accuracy of the regulation mechanism of the promoter. According to the application, the function and the action mechanism of the MaHSF1 and the coding gene thereof for resisting the high-temperature stress damage are verified for the first time, and a solid theoretical basis is provided for the mulberry production to cope with the high-temperature stress damage and the breeding of new high-temperature-resistant varieties.

The invention relates to the field of food processing, in particular to a preparation method of whole-plant fried ginseng and application of the whole-plant fried ginseng in preparation of drugs or food for resisting oxidation and relieving fatigue. The method comprises the following steps: fresh ginseng is taken and cleaned, rhizome, main roots and fibrous roots are separated, the main roots are cut into slices or segments, the rhizome, the slice-shaped main roots or the segment-shaped main roots and the fibrous roots are respectively placed in oil to be fried, and when the rhizome, the slice-shaped main roots or the segment-shaped main roots and the fibrous roots are golden yellow, the rhizome, the main roots and the fibrous roots are fished out, drained off oil and cooled. The antioxidant activity of the fried ginseng slices and segments is improved. Compared with fresh ginseng, the deep-fried ginseng slices and segments can significantly prolong exhaustive swimming time of load-bearing mice, significantly improve liver / muscle glycogen, lactic dehydrogenase content and superoxide dismutase activity, reduce lactic acid, urea nitrogen and malondialdehyde content, and have a significant anti-fatigue effect. According to the method, dryness of fresh ginseng can be removed, the eating fragrance can be improved, the fried ginseng product is instant and convenient to eat, and the fried ginseng product is matched with barbecue kebabs and stewed together with livestock and poultry meat, so that the fried ginseng product is delicious and health-care.

The invention belongs to the technical field of feed additives, and particularly relates to a chicken feed additive for relieving the fatty liver syndrome of laying hens and application of the chicken feed additive. The chicken feed additive is added into daily ration of laying hens; in the daily ration of the laying hens, the addition amount of the tartary buckwheat flavone is 40 mg / kg to 80 mg / kg, and the addition amount of the 25-hydroxy vitamin D3 is 1760 IU / kg to 3760 IU / kg. The chicken feed additive disclosed by the invention can relieve the fatty liver syndrome of laying hens, remarkably reduce the serum level of low-density lipoprotein cholesterol, triglyceride, free fatty acid and malondialdehyde, and remarkably enhance the activity of glutathione peroxidase and superoxide dismutase in the liver at the same time; gene expression associated with fat synthesis and inflammation is significantly inhibited, intestinal microbiota is remodeled, and lipid metabolism homeostasis is maintained to alleviate the fatty liver syndrome of laying hens.

The application discloses a method for relieving corn phytotoxicity caused by triazole fungicide seed dressing, and relates to the technical field of crop plant protection and seed treatment. The application discloses that straw source tobacco water can be used for relieving corn phytotoxicity caused by triazole fungicide seed dressing. The specific application mode is as follows: after the straw source tobacco water is diluted to a specific concentration, the straw source tobacco water is compounded with triazole fungicide to prepare a mixed pesticide; and the mixed pesticide is used for seed dressing treatment of corn seeds. Through test verification, the application proves that the straw source tobacco water can improve the germination ability of corn seeds after triazole fungicide seed dressing, promote the growth of corn seedlings, improve the activities of alpha-amylase, plant sucrose and antioxidant enzymes in the corn seeds, increase the content of reduced glutathione, and reduce the contents of malondialdehyde and superoxide anion, so as to relieve the corn phytotoxicity caused by triazole fungicide.

The invention discloses an application of a bifidobacterium animalis subsp. Lactis ZYhyy-003 metagen preparation in preparation of a pet product for improving an antioxidant function, the preservation number of the bifidobacterium animalis subsp. Lactis ZYhyy-003 is CGMCC (China General Microbiological Culture Collection Center) No.31553, and the preservation date is August 5, 2024. The invention further discloses a preparation method of the bifidobacterium animalis subsp. Lactis ZYhyy-003 metagen preparation. The ZYhyy-003 metagen preparation can remarkably improve the activity of superoxide dismutase, glutathione peroxidase and catalase in serum of pets, reduce the content of malonaldehyde and proinflammatory factors TNF-alpha, IL-1beta and IL-6, improve the content of an anti-inflammatory factor IL-10, increase the weight of the pets, improve the skin state and prevent the pets from suffering from inflammation. The body immune function is synergistically improved while the oxidation resistance of the pets is enhanced. The metagen preparation is safe and stable, is easy to store and produce, fills the technical blank that single strain metagen directionally acts on the pet antioxidation field, and provides a new technical support for development of pet dog antioxidation and immune regulation related functional products.

The invention belongs to the technical field of functional feed additives, and particularly relates to an anti-aging composition for pets as well as a use method and application of the anti-aging composition. The anti-aging composition for pets is prepared from the following raw materials: alpha-ketoglutarate, ergothioneine and spermidine. The use method of the functional feed additive comprises the following steps: weighing the raw materials, uniformly mixing, and adding the functional feed additive into basic ration of dogs and cats. In order to overcome the defects that an existing pet aging delaying product is limited in effect due to single component, poor in adaptability and the like, the anti-oxidation and anti-inflammatory effects are enhanced through synergism of the three components, the activity of superoxide dismutase and glutathione peroxidase in the bodies of dogs and cats can be remarkably improved, and the content of malonaldehyde and proinflammatory factors IL-1beta, IL-6 and TNF-alpha is reduced. The composition is high in absorption and utilization rate, safe and free of burden after being fed for a long time, capable of effectively delaying the aging process of old dogs and cats and improving the life quality of the old dogs and cats, and has important application value.

The invention discloses salt-tolerant marsh kocuria with a plant growth promoting effect and application of the salt-tolerant marsh kocuria, and belongs to the field of microorganism application. The Kocuria palustris is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 21, 2025, and the preservation number is CGMCC No.35324. The Kocuria palustris can be used for preparing a bacterial strain. The Kocuria palustris has the growth promoting capabilities of fixing nitrogen, dissolving phosphorus, producing siderophore, dissolving potassium and the like. Under the stress of 500mM salt, the inoculation of the strain can effectively promote the growth of corn plants, promote the synthesis of soluble sugar, antioxidant enzymes (such as peroxidase and superoxide dismutase) and proline and inhibit the generation of malondialdehyde. Therefore, the strain can be used as an excellent strain for developing a microbial fertilizer and has a good application prospect.

The application discloses application of a GsRZ1A gene in improving plant alkali tolerance, and belongs to the technical field of genetic engineering.The nucleic acid sequence of the GsRZ1A gene is shown as SEQ ID NO:1, and the amino acid sequence coded by the GsRZ1A gene is shown as SEQ ID NO:2.A transgenic strain is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans.The transgenic strain is subjected to alkali stress treatment, and the results show that the soybean strain overexpressing the GsRZ1A gene has a faster relative growth rate, the total leaf area, the chlorophyll content of leaves and the relative water content are higher than those of the control strain transformed with an empty vector, the total root length and the total root surface area of the root system are also significantly greater than those of the control group, and the GsRZ1A gene can improve the antioxidant enzyme activity, reduce the accumulation of superoxide anions and malondialdehyde, and increase the accumulation of osmotic regulation substances such as soluble sugar to enhance the alkali stress tolerance of soybeans, and therefore has an important application prospect in the breeding of alkali-tolerant plants.

The present application relates to a kind of soybean polypeptide preparation method with antioxidant and antagonistic lead damage effect.The soybean polypeptide prepared in the present application has good DPPH free radical clearance rate, ABTS free radical clearance rate and Fe3+ reduction capacity in vitro;It can also alleviate the case that the body weight of mouse is slowly increased due to lead exposure, improve the learning and memory ability of lead exposure mouse, alleviate the organ damage of mouse caused by lead exposure, enhance the level of reduced glutathione, superoxide dismutase and catalase of organism, and alleviate the abnormal increase of malondialdehyde caused by lead exposure.The soybean polypeptide prepared in the present application has good in vitro antioxidant capacity, and has antagonistic effect on oxidative stress caused by lead exposure mouse, and can be used as non-toxic and harmless natural antioxidant.For the development of natural antioxidant food, it has far-reaching application prospect, and also provides a new feasible idea for the combined application of bioinformatics technology and traditional enzymatic technology.