99 results about "Malonic Aldehyde" patented technology

The invention provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of medicinal preparations. The preparation method comprises the following steps: (1) crushing; (2) extracting; (3) standing and separating; and (4) freezing and filtering to obtain the medicinal liquor. The medicinal liquor is obtained by extracting the traditional Chinese medicine components through the steps of crushing in batches, mixing and extracting, standing, freezing and separating the supernate and the suspension in different separation modes, and the medicinal liquor can better improve the space exploration capability of a mouse aged due to D-galactose, can reduce malondialdehyde MDA, and can improve the immunity of the mouse. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) is improved, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention discloses a method for efficiently screening saline-alkaline tolerant rice. According to the method, controllable indoor saline-alkali environment simulation conditions are constructed, various salts are mixed in proportion to prepare solutions with different salinity gradients, the pH value is adjusted, and temperature, humidity and illumination parameters are accurately controlled; the method comprises the following steps: disinfecting rice seeds, carrying out a germination test, transplanting rice seedlings, and regularly measuring growth indexes; in the treatment process, physiological indexes such as malondialdehyde content, antioxidant enzyme activity, proline content and the like and the expression level of salt and alkali tolerance related genes are detected, key indexes are screened by applying statistics and multivariate analysis methods, and an evaluation system is established for comprehensive score screening. The method can quickly perform saline-alkali stress treatment on a large number of rice seeds or seedlings, is not limited by natural environment, shortens the screening period, can accurately simulate different saline-alkali soil environments, scientifically evaluates the saline-alkali tolerance of rice from multiple dimensions, and provides an efficient and reliable technical means for breeding saline-alkali tolerant rice varieties.

The invention provides a polypeptide and application thereof in preparation of a medicine for preventing and / or treating alcoholic liver injury. The polypeptide is safe and free of side effects, can remarkably reduce the content of malondialdehyde (MDA), improve the activity of superoxide dismutase (SOD), improve the content of glutathione (GSH), reduce the activity of glutamic-pyruvic transaminase (ALT), reduce the activity of aspartate transaminase (AST) and reduce the activity of lactic dehydrogenase (LDH), and is very suitable for preparing the medicine for preventing and / or treating the alcoholic liver injury.

The invention discloses application of burdock root polysaccharide in preparation of a medicine for treating cognitive impairment, and belongs to the technical field of traditional Chinese medicines. Experiments prove that the burdock root polysaccharide can improve cognitive impairment and autonomous exploration ability of D-galactose induced cognitive impairment model mice, increase the activity of superoxide dismutase in serum of the mice, reduce the content of malondialdehyde, reduce the content of hippocampus interleukin-1beta and tumor necrosis factor-alpha in brains of the mice, and improve the cognitive impairment activity of the mice. According to the present invention, with the composition, the loss of the neuron in the hippocampal region of the cognitive impairment model mouse can be reduced so as to achieve the neuron protection effect, such that the good treatment effect on the cognitive impairment can be provided, and the new approach is provided for the development of the cognitive impairment-related drug.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention discloses application of an OsLOX1 gene in regulation and control of drought resistance and seed vigor of rice, and relates to the technical field of plant genetic engineering. According to the invention, the drought stress tolerance of the mutation line with the OsLOX1 knocked out through CRISPR / Cas9 is lower than that of a wild type line, and along with the increase of the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA) and the reduction of the expression quantity of antioxidant enzyme related genes. In addition, it is found that compared with a wild type, the service life of rice seeds is shortened by knocking out OsLOX1, meanwhile, the content of H2O2 and MDA is increased, and the activity of antioxidant enzyme superoxide dismutase (SOD) and catalase (CAT) is reduced; the method can be used for breeding storage-resistant and drought-resistant rice varieties; important gene resources are provided for cultivating drought-resistant rice varieties and prolonging the service life of seeds.

The invention discloses a continuous cropping Chinese cabbage planting method based on a microbial agent and application, and belongs to the technical field of crop cultivation. According to the planting method, Chinese cabbage and garlic are intercropped according to the ratio of 1: 1, and a trichoderma harzianum agent is applied. Results show that the planting method can significantly increase the growth indexes such as the plant height, the root length, the leaf chlorophyll content and the soluble sugar content of the Chinese cabbage; the soluble protein content, the peroxidase activity, the superoxide dismutase activity and the malondialdehyde content of the Chinese cabbage leaves are obviously reduced. Meanwhile, the content of rapidly available phosphorus, rapidly available potassium, effective calcium and other substances in the soil can be increased; the activities of urease, catalase and alkaline phosphatase in the soil are also obviously improved. In addition, the number of fungi, bacteria and actinomycetes in the soil is also remarkably increased. Therefore, according to the planting method, the quality of continuous cropping soil can be improved, Chinese cabbage growth is promoted, and theoretical support is provided for healthy and sustainable development of the Chinese cabbage industry.

The invention belongs to the technical field of molecular biology, and particularly relates to application of an OsSCL25 gene in regulating and controlling salt stress resistance of rice. Under the stress of salt, the survival rate, chlorophyll, fresh weight and dry weight of strain seedlings of the overexpressed OsSCL25 gene are higher than those of a wild type, the content of superoxide dismutase, peroxidase and malondialdehyde is lower than that of the wild type, and the content of proline is remarkably higher than that of the wild type and a mutant. The soluble sugar content of the wild type, the soluble sugar content of the OsSCL25 mutant and the soluble sugar content of the overexpression system are not obviously different under normal treatment, and the soluble sugar content of the overexpression system is obviously higher than that of the wild type and the soluble sugar content of the gene mutant after salt stress, so that the OsSCL25 gene participates in rice salt tolerance regulation. After the gene is over-expressed, the salt tolerance of the rice is obviously improved, so that a rice material suitable for saline-alkali soil planting improvement can be cultivated by utilizing the OsSCL25 through a biotechnology, and a new thought is provided for improving the planting rate of the rice in the saline-alkali soil.

The invention discloses a polypeptide fertilizer beneficial to cold resistance of crops and a preparation method thereof, and belongs to the technical field of agricultural fertilizers, the fertilizer is prepared by taking livestock and poultry blood (preferably pig blood) as a raw material, preparing high iron protein peptide through a compound protease hydrolysis process, and matching with a trace element compound, humic acid and a carrier. The preparation process comprises the steps of enzymolysis preparation of the ferritin peptide, fertilizer mixing, granulation, drying and the like. The polypeptide fertilizer disclosed by the invention takes the high-iron protein peptide as a core, the contained small-molecular amino acid polypeptide can enhance the antioxidant enzyme activity of crops at low temperature and reduce malonaldehyde accumulation, the porphyrin iron can promote chlorophyll synthesis and energy metabolism, and the high-iron protein peptide and the porphyrin iron cooperate to remarkably improve the cold resistance of the crops. Meanwhile, by cooperating with trace elements and humic acid, the cold resistance is enhanced, nutrient absorption of crops is promoted, the yield and quality are improved, multiple effects of resisting cold, improving quality, increasing yield and the like are achieved, the raw materials are wide in source, the cost is low, the preparation process is simple, and large-scale production and application are facilitated.

The invention relates to a probiotic preparation containing a BL11 strain and capable of improving obesity-related metabolism and senescence indexes of middle-aged and elderly people and application of the probiotic preparation. The strain in the probiotic preparation is composed of a bifidobacterium longum subsp. Longum BL11 strain and a pediococcus acidilactici PA53 strain. The two strains can cooperate with each other and promote each other, and have a synergistic effect in the aspect of improving obesity-related metabolic indexes, which is specifically embodied in that: the weight increase and body fat rate of mice fed with high fat diet can be reduced; the low-density lipoprotein level, the total cholesterol level and the triglyceride level are reduced, and the high-density lipoprotein level is improved; fasting blood-glucose, glycosylated hemoglobin level and insulin resistance index are reduced; chronic inflammation is relieved; meanwhile, the composition also has excellent performance in the aspect of improving senescence-related indexes, the content of malondialdehyde in serum can be reduced, the activity of superoxide dismutase and glutathione peroxidase can be improved, and the activity of telomerase can be improved.

The invention discloses a synthetic functional bacterial agent and application of the synthetic functional bacterial agent in salt tolerance and growth promotion of wheat, the synthetic functional bacterial agent is prepared from five strains, namely, Gordonia hydrophora, Lysinibacillus oldseyi, planomicrobium okeanokites, Luteimonas huaabiensis and Brevibacterium epidermidis, and the bacterial agent has the remarkable characteristics of no antagonism among the strains, salt and alkali tolerance, high viable count and the like, and can be applied to salt tolerance and growth promotion of wheat. The germination rate, the seedling length and the root length of the spring wheat can be remarkably increased, and the seedling length and the root length of the winter wheat are also remarkably promoted. Under the condition of salt stress, the microbial inoculum can remarkably improve the activity of catalase, peroxidase and superoxide dismutase of wheat and the total content of chlorophyll, after microbial inoculum treatment, the content of malondialdehyde and proline of wheat is remarkably reduced, and the microbial inoculum plays a role in promoting and protecting spring wheat and winter wheat in the process of resisting saline-alkali stress and has a good application prospect. The method has an influence effect on agricultural development and sustainable development in the arid region.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to application of a vitis amurensis VaWRKY72 gene in improving cold resistance of plants. According to the invention, the cold-resistant effect of the over-expressed vitis amurensis VaWRKY72 gene in regulation and control of arabidopsis thaliana and grape is found for the first time. Through overexpression of the vitis amurensis VaWRKY72 gene in arabidopsis thaliana and grape, it is found that the vitis amurensis VaWRKY72 gene can be used for improving the cold stress resistance by reducing the malondialdehyde content, reducing the electrolyte permeability, improving the proline content and the activity of peroxidase, catalase and superoxide dismutase, eliminating excessive ROS and promoting the expression of cold stress related genes. Therefore, the tolerance of the plant to low-temperature stress is improved.

The invention discloses application of an StPYL16 gene in enhancing plant drought stress resistance, and relates to the technical field of biology. The nucleotide sequence of the gene StPYL16 is as shown in SEQ ID NO. 1. Transient and stable expression of the StPYL16 gene in tobacco can enhance the drought resistance of transgenic plants, resulting in improvement of plant height, stem diameter and root development. In addition, compared with a wild plant, the StPYL16 transgenic tobacco shows relatively low malondialdehyde content, relatively high proline accumulation and relatively strong SOD (superoxide dismutase), POD (peroxidase) and CAT (catalase) activities. Meanwhile, the StPYL16 gene also up-regulates the expression level of stress-related genes in transgenic plants under drought treatment. The discovery shows that the StPYL16 gene plays a positive role in regulating the reaction of potatoes to drought stress, and the drought stress resistance of plants can be improved through overexpression of the StPYL16 gene.

The invention belongs to the technical field of preparation of peptides, and particularly relates to camel placenta hydrolysate as well as a preparation method and application thereof. According to the preparation method of the camel placenta hydrolysate and the preparation method of the camel placenta hydrolysate provided by the invention, the camel placenta hydrolysate has remarkable scavenging activity on DPPH, ABTS, hydroxyl free radicals and iron ions, so that the antioxidant activity is exerted; in a fruit fly acute oxidative damage model, the camel placenta hydrolysate can prolong the survival time of fruit flies in an oxidative stress environment; in a normal fruit fly model, the camel placenta hydrolysate can prolong the life of normal aging fruit flies, improve the action ability of the aging fruit flies and play an anti-aging role by improving the activity of in-vivo antioxidant enzymes such as total superoxide dismutase, copper-zinc superoxide dismutase and catalase and reducing the malondialdehyde level on the premise of not reducing the food intake of the fruit flies. The camel placenta hydrolysate can be used as an ideal auxiliary antioxidant and anti-aging medicine and health food raw material.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium animalis subsp. Lactis, a product and application of the bifidobacterium animalis subsp. Lactis in liver protection. The preservation number of the bifidobacterium animalis subsp. Lactis BB-35 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.27836, and the preservation number of the bifidobacterium animalis subsp. Lactis BB-35 provided by the invention is CGMCC No.27836. The strain can be used for reducing the expression condition of a senescence-related marker p21 in liver cells of mice, and reducing the level of senescence-related secretory phenotype factors, the level of cystatin C and liver function-related biochemical indexes, so that the liver senescence condition of the mice is improved, and the influence on other organs is reduced; in addition, the strain can also improve oxidative stress index malonaldehyde and superoxide dismutase content, reduce inflammatory factor TNF-alpha and IL-6 levels, and provide technical support for preparation of products for preventing, relieving and / or treating liver aging and liver injury.

The invention discloses an application of a pyrrosia lingua aqueous extract in preparation of a medicine for preventing and treating radioactive intestinal injury. Radioactive intestinal injury is a common complication of ionizing radiation, and has main pathological characteristics of intestinal mucosal barrier destruction, excessive release of inflammatory factors, increase of oxidative stress level and hematopoiesis and immune function inhibition. At present, no effective and safe prevention and treatment medicine is available. It is found for the first time that the pyrrosia lingua aqueous extract can inhibit expression (IL-6, TNF-alpha and IL-1beta) of inflammatory factors of radiation-induced MODE-K cells and relieve the oxidative stress level (malondialdehyde (MDA) is reduced, and the content of superoxide dismutase (SOD) and glutathione (GSH) is increased). In animal experiments, the pyrrosia lingua aqueous extract can reduce the levels of TNF-alpha and IL-1beta inflammatory factors in serum of irradiated mice and improve oxidative stress disorders such as MDA increase, SOD and GSH decrease and the like; the reduction of leukocytes and lymphocytes is relieved; the length of intestinal villus is promoted and recovered; the proportion of Ki67 positive cells in a crypt region is increased, so that the regeneration capacity of intestinal epithelial cells is enhanced. The pyrrosia lingua aqueous extract disclosed by the invention provides an experimental basis and an application prospect for developing a novel medicine for preventing and treating the radioactive intestinal injury.

The invention relates to the technical field of microorganisms, and discloses a talaromyces funiculi LD-YGH31 and an application of the talaromyces funiculi LD-YGH31 in salt tolerance. The strain is separated from the root system of halophyte, and the preservation number of the strain is CCTCC NO: M 20251815. After the strain is cultured, the strain and plants are co-cultured, and the growth performance and stress resistance of the plants under salt stress can be remarkably improved. Experiments prove that when the strain and tomato seedlings are co-cultured, under the stress condition of 200 mM NaCl salt, the fresh weight and plant height of the tomato seedlings can be remarkably improved, the activity of superoxide dismutase and peroxidase is improved, the content of malondialdehyde and the relative conductivity are reduced, and the K < + > / Na < + > steady state in plant roots and leaves is maintained. The strain is novel, the application method is simple and easy to implement, and the strain can be developed into a microbial fertilizer for saline-alkali soil crop planting and has remarkable agricultural application value.

The invention belongs to the technical field of microbial agents, and particularly relates to a composite microbial agent, a composite liquid microbial agent and application. The invention provides a complex microbial inoculant, which is prepared from bacillus mojavensis W1, bacillus halotolerans L21 and bacillus subtilis M4, wherein the complex microbial inoculant is prepared from bacillus mojavensis, bacillus halotolerans L21 and bacillus subtilis M4, and the complex microbial inoculant is prepared from bacillus mojavensis, bacillus halotolerans L21 and bacillus subtilis M4. The bacillus mojavensis W1, the bacillus halotolerant L21 and the bacillus subtilis M4 in the complex microbial inoculant interact with one another, so that the defense mechanism of plants can be activated, the activity of superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT) can be improved, and the content of malondialdehyde (MDA) can be reduced, thereby improving the prevention and treatment effect on the root rot of the cornutgionium axentgii.

The invention belongs to the technical field of microbial control, and particularly relates to a salt-tolerant bacillus, a microbial agent, a preparation method, a control method and application. The invention provides a salt-tolerant bacillus L21 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35478. The salt-tolerant bacillus L21 can activate the defense mechanism of plants, improve the activity of defensive enzymes in the plants, and enable the plants to have higher oxidation resistance when encountering pathogenic bacteria, so that ROS accumulation is more effectively reduced, MDA is reduced, and the morbidity of root rot is reduced. The result of the embodiment shows that the bacillus halotolerant L21 can improve the activity of superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT), reduce the content of malondialdehyde (MDA) and reduce the morbidity of root rot of cornutgionium axataenense.

The application discloses application of Ligusticum sinense alcohol extract in preparation of a medicine for preventing or treating drug-induced liver injury, and relates to the technical field of biological medicine.The application proves for the first time that the Ligusticum sinense alcohol extract has an obvious effect of improving acute drug-induced liver injury, can obviously improve serum biochemical indexes, inflammatory factor levels, and liver tissue pathological states of mice with acute drug-induced liver injury, and improve superoxide dismutase (SOD) and glutathione (GSH) activities in liver tissue and reduce malondialdehyde (MDA) and reactive oxygen species (ROS) levels.The pharmacodynamics test proves that the Ligusticum sinense alcohol extract has a significant liver injury protection effect, and has a low effective dose and no toxic side effect.Therefore, the application can become an alternative medicine for preventing and treating drug-induced liver injury.

The invention belongs to the technical field of microbial prevention and control, and particularly relates to bacillus subtilis, a microbial agent, a preparation method, a prevention and control method and application. The invention provides a strain of bacillus subtilis M4, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35477. The bacillus subtilis M4 is used for preventing and treating root rot of cornutgionium axe. The bacillus subtilis M4 can improve the activity of disease-resistant defensin superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT), reduce the content of malondialdehyde (MDA), finally reduce the morbidity and the morbidity index of plant root rot and promote plant growth.

This invention relates to the field of bioengineering technology and provides an application of the maize heat shock transcription factor gene ZmHSF18, wherein the nucleotide sequence of the maize heat shock transcription factor gene ZmHSF18 is shown in SEQ ID NO:1. Experiments have demonstrated that overexpression of the maize heat shock transcription factor gene ZmHSF18 in plants can significantly improve seed germination rate and frozen survival rate at the mature stage under low-temperature stress, reduce malondialdehyde accumulation, and increase superoxide dismutase activity. Therefore, this maize heat shock transcription factor gene ZmHSF18 can be used to improve plant cold tolerance and create transgenic plants with enhanced cold tolerance, possessing important application value in plant genetic engineering and stress resistance breeding.

This invention discloses the application of burdock root polysaccharide in the preparation of drugs for treating cognitive impairment, belonging to the field of traditional Chinese medicine technology. Experiments demonstrate that burdock root polysaccharide can improve cognitive impairment and independent exploration ability in D-galactose-induced cognitive impairment model mice, increase superoxide dismutase activity in mouse serum, reduce malondialdehyde content, decrease the content of interleukin-1β and tumor necrosis factor-α in the hippocampus of mouse brains, and alleviate the loss of neurons in the hippocampus of cognitive impairment model mice, thereby achieving a neuroprotective effect and demonstrating a good therapeutic effect on cognitive impairment. This provides a new approach for developing drugs related to the treatment of cognitive impairment.

The invention discloses sea cucumber active peptide and application thereof, and belongs to the field of food science. The amino acid sequence of the sea cucumber active peptide is GYMLPK, and the sea cucumber active peptide has the functions of oxidation resistance, immunoregulation and intestinal health regulation. The active peptide is extracted from sea cucumber protein by adopting a biological enzymolysis method, and can improve the activity of serum superoxide dismutase (SOD), reduce the content of malondialdehyde (MDA) and promote the growth of intestinal beneficial flora at the same time. The sea cucumber active peptide can be widely applied to functional foods, health supplements and sports nourishments.

The invention discloses an application of a flammulina velutipes extract in preparation of related products for relieving and / or treating drug-induced liver injury. By constructing a drug-induced liver injury model, it is found that by means of the needle mushroom extract rich in needle mushroom polysaccharide in low dosage, liver pathological injuries such as cisplatin-induced hepatocyte swelling and necrosis can be remarkably relieved, the levels of serum liver function indexes of alanine transaminase and aspartate transaminase are reduced, and the liver injury resistance is improved. And meanwhile, the activity of superoxide dismutase and glutathione peroxidase in liver tissues is improved, the concentration of malonaldehyde is reduced, and excellent antioxidant characteristics are shown. In addition, the invention also discovers that the flammulina velutipes extract can improve the composition of intestinal microorganisms, promote colonization of beneficial bacteria, reduce the abundance of harmful bacteria and regulate the key metabolic pathway of the liver at the same time, and can provide a treatment strategy for the treatment of drug-induced liver injury.

The invention relates to the technical field of plant stress resistance regulation and control and the field of plant genetic engineering, in particular to application of strigolactones GR24 or induced protein CsJIP-like thereof in improving cold resistance of tea trees, a strigolactones GR24 solution is sprayed to the tea trees, the relative conductivity of the tea trees and the content of malondialdehyde, hydrogen peroxide and superoxide anions can be reduced, and the cold resistance of the tea trees can be improved. The activity of superoxide dismutase, peroxidase, catalase and ascorbic acid peroxidase in the tea trees is improved, the oxidative stress of the tea trees is relieved, the integrity of cell membranes is protected, cell osmotic equilibrium is maintained, and dehydration damage caused by low temperature is avoided; screening a cold-resistant gene CsJIP-like responding to GR24, and verifying that the cold-resistant gene CsJIP-like has a cold-resistant function by utilizing instantaneous overexpression and instantaneous silencing of tea tree leaves; the GR24 is high in chemical stability, small in application amount, environmentally friendly and beneficial to promoting the genetic improvement process of cold resistance of the tea trees.

The invention discloses calf animal coccus PD13 for improving Alzheimer's disease and application of the calf animal coccus PD13, and relates to the technical field of biology. Experiments prove that in an Alzheimer's disease model mouse induced by injecting A beta oligomer into the brain, the Azococcus calvus PD13 can improve spatial learning and memory disorder and autonomous exploration ability of the AD model mouse and relieve damage to neurons in a hippocampal region of the Alzheimer's disease model mouse, so that the effect of protecting the neurons is achieved, and the Azococcus calvus PD13 can be used for preparing a medicine for treating the Alzheimer's disease. According to the present invention, with the application of the animal coccus calves, the content of glutathione in mouse serum and the enzyme activity of superoxide dismutase are increased, and the content of malondialdehyde is reduced, such that the animal coccus calves provides good prevention and treatment effects on the Alzheimer's disease, and the new approach is provided for developing the Alzheimer's disease-related drugs.

The invention discloses an application of ligularia virgaurea alcohol extract in preparation of a medicine for preventing or treating drug-induced liver injury, relates to the technical field of biological medicines, and proves that the ligularia virgaurea alcohol extract has an obvious effect of improving acute drug-induced liver injury for the first time. The traditional Chinese medicine composition can obviously improve serum biochemical indexes, inflammatory factor levels and hepatic tissue pathological states of mice with acute drug-induced liver injury, improve the activity of superoxide dismutase (SOD) and glutathione (GSH) in hepatic tissues, and reduce the levels of malondialdehyde (MDA) and reactive oxygen species (ROS). Pharmacodynamic tests prove that the ligularia nobilis alcohol extract has a remarkable liver injury protection effect, and is low in effective dose and free of toxic and side effects. Therefore, the invention may become an alternative drug for preventing and treating drug-induced liver injury.

The present invention provides an application of chitosan oligosaccharide aminomethylthiobutyramide derivatives in plant resistance to salt stress, belonging to the field of agricultural biotechnology. The derivatives are used to alleviate the oxidative damage of plants caused by salt stress, specifically including the following effects: (1) reducing the malondialdehyde content in plants; (2) increasing the activities of superoxide dismutase and peroxidase; and (3) promoting seed germination and seedling growth. The derivatives are formed by connecting the carboxyl group of methionine with the amino group of chitosan oligosaccharide through an amide bond, and the derivatives are 2-amino-4-methylthiobutyryl chitosan oligosaccharide, which is a methionine high-molecular chitosan oligosaccharide with a deacetylation degree of ≥90%. The polymerization degree of the methionine high-molecular chitosan oligosaccharide is 10-18. The present invention provides a methionine carrier for loading methionine into plants to improve the effect of plant resistance to salt stress.

The invention relates to application of germplasm of botryosphaeria formosa to preparation of health-care products for improving antioxidation, and belongs to the technical field of functional evaluation of health-care foods, the germplasm of botryosphaeria formosa promotes expression of superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-PX) by inhibiting accumulation of peroxide malonaldehyde (MDA) in heart, liver and serum, so that the anti-oxidation effect of the botryosphaeria formosa is improved, and the anti-oxidation effect of the botryosphaeria formosa is improved. The anti-oxidation regulation function is realized. The boil bean germplasm is extracted for 30 minutes by adopting boiling water, so that the use of an organic solvent is avoided to retain natural active ingredients, and the boil bean milk containing 1g / mL of crude drug is obtained and is used for preparing antioxidant health-care products. The health-care product is suitable for prevention or adjuvant treatment of diseases caused by oxidative stress, such as cardiovascular diseases, liver injury or aging related diseases. Experiments prove that the anti-oxidation effect of the germplasm of the botryosphaeria formosa is evaluated, the germplasm of the botryosphaeria formosa has a reliable anti-oxidation regulation effect, the MDA level in the heart, the liver and serum can be remarkably reduced, and the expression of SOD, GSH and GSH-PX is promoted.