80 results about "Cholesterol total" patented technology

The invention discloses a composition of probiotic fermented horseradish tree leaves as well as a preparation method and application of the composition. The preparation method comprises the following steps: crushing and immersing horseradish tree leaves; adding glucose, peptone, yeast extract, yeast powder and inorganic salt into an immersing solution; extracting at a high pressure; and after carrying out probiotic fermentation and centrifuging, adding polydextrose and fructo-oligosaccharide syrup and sterilizing to obtain the composition of the probiotic fermented horseradish tree leaves. The composition can be used for preparing health products, foods and beverages capable of lowering blood fat and improving a sleeping function. An experiment shows that the composition of the probiotic fermented horseradish tree leaves can be used for reducing the contents of total cholesterol (TC) and triglyceride (TG) in blood serum, and improving the content of high density lipoprotein cholesterol (HDL-C) in the blood serum; the composition of the probiotic fermented horseradish tree leaves, disclosed by the invention, has a function of lowering a blood fat level.

Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed. Assays may be performed in a single reaction mixture, allowing more accurate and precise cholesterol determinations, including ratios of cholesterol sub-fractions to total cholesterol, at less expense, than would be expected by performing several different assays in different reaction mixtures.

The invention discloses an application of lactobacillus rhamnosus CCFM1060 in preparation of functional microbial agents, foods and/or medicines. The lactobacillus rhamnosus CCFM1060 can tolerate thegastrointestinal environment of the human body, significantly improve fasting blood glucose rise and abnormal oral glucose tolerance of type 2 diabetics caused by high fat diet and STZ injection, reduce the area under the curve during glucose tolerance drug administration, and relieve insulin resistance; remarkably improve the increase of total cholesterol content and the increase of low-density lipoprotein cholesterol in serum of type 2 diabetics caused by high-fat diet and STZ injection; obviously relieve the inflammatory state of the liver; and significantly reduce pancreatic and liver pathological injuries caused by high-fat diet and STZ injection. The lactobacillus rhamnosus CCFM1060 has relatively strong adsorption capacity on perfluorooctanoic acid (PFOA), and has the capacity of relieving the toxicity of the PFOA.

The invention discloses a method for determining cholesterol by using flow injection chemiluminescence with nano-copper oxide as a catalyst. The method comprises the following steps: mixing cholesterol, cholesterol oxidase and phosphatic buffer solution; warm bathing the mixture solution to obtain a reaction solution; after diluting the reaction solution, inputting the diluted reaction solution into a mixer through a peristaltic pump to mix with a nano-copper oxide colloidal solution; then mixing with a luminol solution; entering into a flow cell for reaction; and detecting the generated chemiluminescence intensity by a photomultiplier to determine the cholesterol. By using cholesterol oxidase for catalyzing the oxidation of cholesterol to generate hydrogen peroxide, combining the flow injection technique, the chemiluminescence signal in the determination of cholesterol is enhanced about 600 times. By using cholesterol-cholesterol oxidase-luminol-nano-copper oxide flow injection chemiluminescence to determine the cholesterol content, the linear range is 0.625-12.5 [mu]mol/L. the method can be used for determination of food cholesterol content and determination of the free cholesterol and total cholesterol in serum.

The invention provides a new medical application of a Chinese traditional medicine fructus ligustri lucidi and the producing method of the extract thereof, which is produced by following steps: ethanol circumfluence, extracting and concentrating from the plant fructus ligustri lucidi to get an ethanol extract, separation by crystallization with the hydrochloric acid function, NaOH treating, again acid out, washing, drying, obtaining other effective components. Pharmacological experiments prove that the medicine can be used for treating the II-type diabetes and induced complication, which has the effects such as reducing the blood sugar, triglyceride, total cholesterin, and low density lipoprotein, and increasing the high density lipoprotein and etc. Furthermore, it can avoid the weight decrease and has a long action and no side effects.

The invention relates to the technical field of microbes, and discloses a probiotic composition and application thereof. The probiotic composition consists of bifidobacterium adolescentis CCFM8630, lactobacillus reuterii CCFM8631 and lactobacillus rhamnosus CCFM1044, the effect of relieving metabolic syndromes is significantly better than that of CCFM8630 or CCFM8631 used alone and in combination,especially in fields of reducing an epididymal fat content, reducing a fasting blood glucose level, reducing a glucose tolerance curve lower area, lowering a serum low-density lipoprotein and total cholesterol content, increasing the liver antioxidant capacity and reducing a serum IL-1beta content, the decrease or increase of the composition is increased by 9.62%-769.62% compared with a single bacteria or double bacteria combination formula, and the triple bacteria combination can achieve significant synergy.

The invention provides honeysuckle flower vinegar drink, prepared from the following components in percentage by mass: 5 to 20 percent of honeysuckle flower vinegar drink raw liquid, 10 to 30 percent of honeysuckle flower distillate, 1 to 5 percent of honey and the balance of water, wherein the honeysuckle flower vinegar drink raw liquid is obtained by performing cellulase enzymolysis, yeast fermentation and acetic bacteria fermentation on honeysuckle flower distillation residue in seuqnece. The obtained honeysuckle flower vinegar drink is rich in aroma, mild in vinegar smell and gentle in mouth feel. Animal tests show that after the honeysuckle flower vinegar drink is poured into the stomach of an SD mouse, total cholesterol, triglycerides and low density lipoprotein of the mouse are all remarkably lowered; compared with the effects of a lipid-lowering weight-reducing medicine orlistat, the honeysuckle flower vinegar drink has the advantages that the effects of lowering the total cholesterol and the low density lipoprotein of the mouse are not remarkably different, and the effect of lowering the triglycerides of the mouse is obviously higher than that of the orlistat.

The invention discloses compound nanoemulsion oral liquid for reducing the blood fat. The particle size of the oral liquid is 1nm-100nm. The compound nanoemulsion oral liquid comprises the following components in percentage by weight: 2%-10% of EPA deep-sea fish oil, 0.1%-4% of carboxymethyl chitosan, 1%-3% of taurine, 0.1%-3% of apricot kernel oil, 0.1%-3% of perilla oil, 0.1%-1% of natural vitamin E, 30%-60% of surfactant, 1%-10% of cosurfactant, 1-6% of oil phase and the balance of deionized water, and the sum of the mass percents of all the components is 100%. The compound nanoemulsion oral liquid can reduce the total cholesterol of the blood, the low-density lipoprotein, the triglyceride and the absorption of lipid simultaneously, has an obvious effect for reducing the blood fat and plays a positive role in preventing the cardiovascular diseases such as cerebral thrombosis, cerebral hemorrhage and high blood pressure and the like.

The invention provides a traditional Chinese medicine for curing type II diabete, which is Hubei dwarf lilyturf tuber hetetopolysaccharide extracted by taking Hubei dwarf lilyturf tuber as a raw material. The traditional Chinese medicine has obvious effects of reducing blood sugar of mice with the type II diabete, obvious effects of reducing insulin resistance and the effect strength of the traditional Chinese medicine has no statistical difference from that of rosiglitazone. Compared with the rosiglitazone, the hetetopolysaccharide can better reduce blood total cholesterol and triglycercide, improve ratio of high-density lipoprotein to low-density lipoprotein, and effectively prevent and curing arteriosclerosis and thrombosis.

The invention provides a total cholesterol detection reagent with high accuracy and disturbance resisting capacity and relates to the technical field of total cholesterol detection. A biological buffer CAPS (3-(hexanaphthene amino)-1-propanesulfonic acid) is used in the reagent R, and therefore the buffering ability is ensured; a surfactant octadecy trimethyl ammonium bromide and a surfactant EMULGEN-707 are added into the reagent R, the emulsification effect of the reagent is improved well, and the accuracy and specificity of the reagent are improved; stabilizers such as trehalose and mannitol are added into the reagent R, and the stability of the reagent can be improved effectively; hydroxy ethidene diphosphonic acid is added into the reagent R, heavy metal ions can be effectively chelated, and the accuracy of the reagent can be improved; chemical-resistant succinoxidase and bilrubin oxidase are added into the reagent, disturbance of ascorbic acid and bilirubin can be effectively removed, and the reagent is simple in preparation, low in price and very suitable for large-area clinical popularization.

The present invention discloses a blood fat three-item test card. The blood fat three-item test card comprises an upper cover (100), blood fat detection test paper (200) and a base seat (300); a sample adding hole (101) is formed in the upper cover (100); three detection holes are formed in the base seat (300), namely a first detection hole (301a), a second detection hole (301b) and a third detection hole (301c) corresponding to three modules of the blood fat detection test paper respectively; and the components of the blood fat detection test paper sequentially comprises a filter layer (201),a diffusion layer (202), a blood filter layer (203), an interception layer (204) and a reaction layer (205) from top to bottom. The blood fat test card is convenient to operate and high in anti-interference capability, and can detect total cholesterol, triglyceride and high-density lipoprotein cholesterol in human blood.

The invention discloses application of a traditional Chinese medicine composition in preparation of drugs for preventing and treating abnormal fat metabolism diseases. The traditional Chinese medicinecomposition is prepared from herba artemisiae scopariae, radix bupleuri, radix sophorae flavescentis, radix et rhizoma rhei and folium isatidis. Through screening of a large number of experiments, the traditional Chinese medicine composition provided by the invention has a good effect of reducing the content of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) of a hyperlipidemia animal model, and has a certain effect of increasing high-density lipoprotein cholesterol (HDL-C). The clinical experiments find that the Kuhuang granules can obviously improvebiochemical indexes and imaging evaluation results of fatty liver patients. In addition, the experimental result of the drug-induced liver injury shows that the traditional Chinese medicine composition provided by the invention can significantly improve the drug-induced liver injury and has the effect of preventing the liver injury.

Preparation of azetidinone compounds and medical use thereof are provided by the present invention. More particularly, azetidionne compounds, shown as formula (I), wherein R¹, R², R³, R⁴, R⁵ and R⁶ are defined in description, and preparation methods thereof are provided by the present invention. The compounds of the present invention can reduce the levels of total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) in plasma, and can be used as medicaments for reducing cholesterol in blood. Therefore the compounds of the present invention can be used to treat or prevent diseases of atherosclerosis, cacergasia of blood vessel, cardiac failure, coronary artery disease, angiocardiopathy, myocardial, angina, hyperlipoidemia and hypercholesteremia and the like. Preparation method of compounds of formula (I) and intermediate compounds are also provided by the present invention.

The present invention provides blood-lipid-lowering food. The blood-lipid-lowering food is characterized by comprising the following materials of cassia seed powder, alismatis rhizome powder, haw powder and konjac soluble dietary fibers. The blood-lipid-lowering food has an effect of lowering serum triglyceride (TG) content to 1.45 plus or minus 0.17 mmol/L and total cholesterol content to 4.55 plus or minus 0.11 mmol/L after hyperlipidemia populations with serum total cholesterol (TC) content of 6.5-7.0 mmol/L or serum triglyceride (TG) content of 2.5-3 mmol/L eating the food for 6 months.

The invention belongs to a method for screening an effective hypolipidemic component in medicated edible fungus by establishing a foam cell model in cytobiology. The method comprises the following steps of: inducing a macrophage RAW264.7 by using an oxidized low-density lipoprotein (OX-LDL) to cause fatty degeneration to obtain a foam cell; combining oil red O staining and measuring the content of total cholesterol and the contents of free cholesterol and cholesterol ester in the cell to detect the effusion condition of cholesterol in the cell to achieve the effects of checking whether the macrophage has fatty degeneration and damage or not and treating the macrophage having fatty degeneration by use of medicines; and detecting and screening the effective hypolipidemic component in the edible fungus through detecting the treatment effect on the foam cell model.

The invention belongs to the technical field of solid beverages, and particularly relates to a high-protein probiotic solid beverage. The high-protein probiotic solid beverage comprises the following component raw materials in parts by weight: 65-70 parts of soybean protein isolate, 15-20 parts of whole milk powder, 15-26 parts of resistant dextrin, 15-20 parts of maltooligosaccharide powder, 10 parts of oat crisp grains, 15-25 parts of nonfat dry milk powder, 10-15 parts of whey protein and 10-15 parts of fish collagen peptide powder. The high-protein probiotic solid beverage disclosed by the invention has the beneficial effects that the soybean protein isolate is used as a main material, so that the intake of cholesterol and saturated fat is reduced, and besides, the balance of nutrient intake is also achieved; the soybean protein can prevent urinary calcium loss, promote bone health, reduce the probability of suffering from osteoporosis, and effectively reduce the content of total cholesterol and low-density lipoprotein cholesterol in blood of human bodies, so that the risk of suffering from heart diseases is reduced; and besides, original nutrition inhibiting factors namely trypsin inhibitors in soybeans are removed from the soybean protein isolate powder, so that people do not need to worry about adverse reactions of indigestion, gaseous distention and the like.

The invention provides applications of a grapefruit extract in preparation of lipid-lowering functional food, healthy food or medicines. The grapefruit extract is prepared by the following steps: performing acetone ultrasonic extraction, rotary evaporation, solid-phase extraction column passing and vacuum drying to freeze-dried powder of extracts of a grapefruit oil gland layer, an albedo layer and a mesocarp layer. The body weight of high-lipid induced fat C57BL/6 mouse can be reduced in vivo through the grapefruit extract, the masses of the liver and adipose tissues can be obviously decreased, the triglyceride, total cholesterol and low-density lipoprotein cholesterol containing in serum can be obviously reduced, and the triglyceride and total cholesterol contained in the liver can be reduced. The grapefruit extract can be used for prevention and treatment of fat metabolism abnormality related diseases as functional food, healthy products or preventive and treatment drugs.

The invention relates to preparation of a composition having the effect of assisting in reducing blood lipid. The composition comprises the following components in parts by weight of 2-50 parts of chenopodium quinoa willd powder, 1-30 parts of soybean protein powder, 0.1-2 parts of spirulina powder, 0.1-2 parts of a natto extract and 0.1-2 parts of a bitter gourd extract. In a preferred scheme, 0.0001-0.001 part of a folic acid compound, 0.1-3 parts of green tea powder and 0.1-3 parts of corn stigma powder are also added to the composition. The composition disclosed by the invention can be used for preparing various preparations having the effect of assisting in reducing blood lipid. The composition not only has obvious efficacy of assisting in reducing the content of total cholesterol (TC) and triglyceride(TG) in serum, and increasing the content of high density lipoprotein cholesterol (HDL-C), but also has the advantages of being long in storage time and small in side effects, and issuitable for various crowds of various ages to use.