91 results about "Cholesterol total" patented technology

A multilayer test strip that measures concentrations of multiple analytes from a single whole blood sample. The test strip includes a test matrix of several layers held together in constant contact by a test strip holder. The invention is characterized in that it has no moving parts, which is made possible by the novel use of an elongate disbursement layer that spreads blood throughout its entire length, despite having layers with known wicking properties adjacent to and in contact with it. Since the invention relies primarily on a vertical flow format, the test strip is advantageously quite compact. With a single 35 microliter sample of blood applied thereto, the novel test strip can provide readings of total cholesterol, HDL cholesterol and triglycerides. From these, LDL can be calculated, thereby providing a full “lipid panel.” Other analytes such as glucose and ketones may be included in the test strip in addition to or in lieu of one or more of the other analytes.

The invention discloses a composition of probiotic fermented horseradish tree leaves as well as a preparation method and application of the composition. The preparation method comprises the following steps: crushing and immersing horseradish tree leaves; adding glucose, peptone, yeast extract, yeast powder and inorganic salt into an immersing solution; extracting at a high pressure; and after carrying out probiotic fermentation and centrifuging, adding polydextrose and fructo-oligosaccharide syrup and sterilizing to obtain the composition of the probiotic fermented horseradish tree leaves. The composition can be used for preparing health products, foods and beverages capable of lowering blood fat and improving a sleeping function. An experiment shows that the composition of the probiotic fermented horseradish tree leaves can be used for reducing the contents of total cholesterol (TC) and triglyceride (TG) in blood serum, and improving the content of high density lipoprotein cholesterol (HDL-C) in the blood serum; the composition of the probiotic fermented horseradish tree leaves, disclosed by the invention, has a function of lowering a blood fat level.

Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed. Assays may be performed in a single reaction mixture, allowing more accurate and precise cholesterol determinations, including ratios of cholesterol sub-fractions to total cholesterol, at less expense, than would be expected by performing several different assays in different reaction mixtures.

The invention relates to a new use of lactobacillus for treating type II diabetes mellitus, wherein the lactobacillus comprises viable bacteria, dead bacteria or a culture product supernatant of lactobacillus reuteri GMNL-89 having the preservation number of CCTCC M207154, and a pharmaceutically acceptable carrier. And the invention discloses the strain having the efficacies of resisting oxidative enzyme activity, resisting inflammation, lowering blood glucose, lowering triglycerides, lowering total cholesterols, reducing density lipoprotein, improving high-density lipoprotein, enhancing the utilization rate of insulin and the like in a type II diabetes mellitus animal mode.

The invention discloses an application of lactobacillus rhamnosus CCFM1060 in preparation of functional microbial agents, foods and / or medicines. The lactobacillus rhamnosus CCFM1060 can tolerate thegastrointestinal environment of the human body, significantly improve fasting blood glucose rise and abnormal oral glucose tolerance of type 2 diabetics caused by high fat diet and STZ injection, reduce the area under the curve during glucose tolerance drug administration, and relieve insulin resistance; remarkably improve the increase of total cholesterol content and the increase of low-density lipoprotein cholesterol in serum of type 2 diabetics caused by high-fat diet and STZ injection; obviously relieve the inflammatory state of the liver; and significantly reduce pancreatic and liver pathological injuries caused by high-fat diet and STZ injection. The lactobacillus rhamnosus CCFM1060 has relatively strong adsorption capacity on perfluorooctanoic acid (PFOA), and has the capacity of relieving the toxicity of the PFOA.

The invention relates to the technical field of medicine, particularly to applications of chitosan oligosaccharide. According to the present invention, chitosan oligosaccharide is applied in preparation of drugs with functions of weight reduction and / or body fat reduction, and chitosan oligosaccharide with a number average molecular weight of not more than 3000 is preferably selected, wherein the chitosan oligosaccharide with different number average molecular weights provides significant application effects in body weight control, body fat ratio reduction, and reduction of total cholesterol and / or low density lipoprotein cholesterol in blood serum. In addition, the chitosan oligosaccharide has effects of weight reduction, body fat reduction, fatty liver alleviating and reduction of blood fat in blood serum.

The invention discloses a method for determining cholesterol by using flow injection chemiluminescence with nano-copper oxide as a catalyst. The method comprises the following steps: mixing cholesterol, cholesterol oxidase and phosphatic buffer solution; warm bathing the mixture solution to obtain a reaction solution; after diluting the reaction solution, inputting the diluted reaction solution into a mixer through a peristaltic pump to mix with a nano-copper oxide colloidal solution; then mixing with a luminol solution; entering into a flow cell for reaction; and detecting the generated chemiluminescence intensity by a photomultiplier to determine the cholesterol. By using cholesterol oxidase for catalyzing the oxidation of cholesterol to generate hydrogen peroxide, combining the flow injection technique, the chemiluminescence signal in the determination of cholesterol is enhanced about 600 times. By using cholesterol-cholesterol oxidase-luminol-nano-copper oxide flow injection chemiluminescence to determine the cholesterol content, the linear range is 0.625-12.5 [mu]mol / L. the method can be used for determination of food cholesterol content and determination of the free cholesterol and total cholesterol in serum.

The invention provides a new medical application of a Chinese traditional medicine fructus ligustri lucidi and the producing method of the extract thereof, which is produced by following steps: ethanol circumfluence, extracting and concentrating from the plant fructus ligustri lucidi to get an ethanol extract, separation by crystallization with the hydrochloric acid function, NaOH treating, again acid out, washing, drying, obtaining other effective components. Pharmacological experiments prove that the medicine can be used for treating the II-type diabetes and induced complication, which has the effects such as reducing the blood sugar, triglyceride, total cholesterin, and low density lipoprotein, and increasing the high density lipoprotein and etc. Furthermore, it can avoid the weight decrease and has a long action and no side effects.

The invention relates to the technical field of microbes, and discloses a probiotic composition and application thereof. The probiotic composition consists of bifidobacterium adolescentis CCFM8630, lactobacillus reuterii CCFM8631 and lactobacillus rhamnosus CCFM1044, the effect of relieving metabolic syndromes is significantly better than that of CCFM8630 or CCFM8631 used alone and in combination,especially in fields of reducing an epididymal fat content, reducing a fasting blood glucose level, reducing a glucose tolerance curve lower area, lowering a serum low-density lipoprotein and total cholesterol content, increasing the liver antioxidant capacity and reducing a serum IL-1beta content, the decrease or increase of the composition is increased by 9.62%-769.62% compared with a single bacteria or double bacteria combination formula, and the triple bacteria combination can achieve significant synergy.

The invention provides honeysuckle flower vinegar drink, prepared from the following components in percentage by mass: 5 to 20 percent of honeysuckle flower vinegar drink raw liquid, 10 to 30 percent of honeysuckle flower distillate, 1 to 5 percent of honey and the balance of water, wherein the honeysuckle flower vinegar drink raw liquid is obtained by performing cellulase enzymolysis, yeast fermentation and acetic bacteria fermentation on honeysuckle flower distillation residue in seuqnece. The obtained honeysuckle flower vinegar drink is rich in aroma, mild in vinegar smell and gentle in mouth feel. Animal tests show that after the honeysuckle flower vinegar drink is poured into the stomach of an SD mouse, total cholesterol, triglycerides and low density lipoprotein of the mouse are all remarkably lowered; compared with the effects of a lipid-lowering weight-reducing medicine orlistat, the honeysuckle flower vinegar drink has the advantages that the effects of lowering the total cholesterol and the low density lipoprotein of the mouse are not remarkably different, and the effect of lowering the triglycerides of the mouse is obviously higher than that of the orlistat.

The invention discloses compound nanoemulsion oral liquid for reducing the blood fat. The particle size of the oral liquid is 1nm-100nm. The compound nanoemulsion oral liquid comprises the following components in percentage by weight: 2%-10% of EPA deep-sea fish oil, 0.1%-4% of carboxymethyl chitosan, 1%-3% of taurine, 0.1%-3% of apricot kernel oil, 0.1%-3% of perilla oil, 0.1%-1% of natural vitamin E, 30%-60% of surfactant, 1%-10% of cosurfactant, 1-6% of oil phase and the balance of deionized water, and the sum of the mass percents of all the components is 100%. The compound nanoemulsion oral liquid can reduce the total cholesterol of the blood, the low-density lipoprotein, the triglyceride and the absorption of lipid simultaneously, has an obvious effect for reducing the blood fat and plays a positive role in preventing the cardiovascular diseases such as cerebral thrombosis, cerebral hemorrhage and high blood pressure and the like.

The invention relates to a benzothiazole derivative, a preparation method and an application thereof, wherein the benzothiazole derivative is 2-m-aniline benzothiazole-1H-pyrazole-3-formamide, and the structural formula of the benzothiazole derivative is shown in the description. The benzothiazole derivative disclosed by the invention can accelerate the growth of saccharomycetes, strengthen the metabolic activity of the saccharomycetes, obviously reduce the plasma triglyceride, the total cholesterol and the high-density lipoprotein of C57 BL / 6J mice fed with high-fat feedstuff, reduce the systolic pressure and the diastolic pressure of model mice, reduce the fasting blood glucose of the model mice, improve the sugar tolerance of the mice, affect the expression of SIRT1 and relevant genes (FOXO1, P53, PPAB gamma and PGC-1 alpha), and obviously improve the sugar and fat metabolism of diabetic model mice. Therefore, the benzothiazole derivative can be applied to preparing medicines for preventing and treating diabetes mellitus and complications of the diabetes mellitus.

The invention provides a traditional Chinese medicine for curing type II diabete, which is Hubei dwarf lilyturf tuber hetetopolysaccharide extracted by taking Hubei dwarf lilyturf tuber as a raw material. The traditional Chinese medicine has obvious effects of reducing blood sugar of mice with the type II diabete, obvious effects of reducing insulin resistance and the effect strength of the traditional Chinese medicine has no statistical difference from that of rosiglitazone. Compared with the rosiglitazone, the hetetopolysaccharide can better reduce blood total cholesterol and triglycercide, improve ratio of high-density lipoprotein to low-density lipoprotein, and effectively prevent and curing arteriosclerosis and thrombosis.

The invention provides a total cholesterol detection reagent with high accuracy and disturbance resisting capacity and relates to the technical field of total cholesterol detection. A biological buffer CAPS (3-(hexanaphthene amino)-1-propanesulfonic acid) is used in the reagent R, and therefore the buffering ability is ensured; a surfactant octadecy trimethyl ammonium bromide and a surfactant EMULGEN-707 are added into the reagent R, the emulsification effect of the reagent is improved well, and the accuracy and specificity of the reagent are improved; stabilizers such as trehalose and mannitol are added into the reagent R, and the stability of the reagent can be improved effectively; hydroxy ethidene diphosphonic acid is added into the reagent R, heavy metal ions can be effectively chelated, and the accuracy of the reagent can be improved; chemical-resistant succinoxidase and bilrubin oxidase are added into the reagent, disturbance of ascorbic acid and bilirubin can be effectively removed, and the reagent is simple in preparation, low in price and very suitable for large-area clinical popularization.

The invention relates to the technical field of medicine, particularly to applications of chitosan oligosaccharide. According to the present invention, chitosan oligosaccharide is applied in preparation of drugs with functions of weight reduction and / or body fat reduction, and chitosan oligosaccharide with a number average molecular weight of not more than 3000 is preferably selected, wherein the chitosan oligosaccharide with different number average molecular weights provides significant application effects in body weight control, body fat ratio reduction, and reduction of total cholesterol and / or low density lipoprotein cholesterol in blood serum. In addition, the chitosan oligosaccharide has effects of weight reduction, body fat reduction, fatty liver alleviating and reduction of blood fat in blood serum.

The present invention relates to compositions comprising Lactobacillus plantarum 2830 (ECGC 13110402), or mutant strain or strains thereof, for use in the treatment or prevention of hypercholesterolaemia, and in particular reducing the total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) levels, in an individual. Specific dosage regimes and methods of production are also claimed and described.

The invention discloses fu tea extract and application thereof in regulating blood lipid and liver fat. The method for preparing the fu tea extract comprises the following steps that with fu tea beinga raw material, organic solvent or CO2 is used for extraction, and after the extraction is completed, the fu tea extract is obtained. A medicine effect test shows that after the fu tea extract is therapeutically applied to a model of ICR mice fed by high-fat feed for four weeks, when the tested extract is at the high dosage of 500 mg / kg, the fu tea extract has the obvious effects on lowering blood lipid relevant important indexes of total cholesterol, triglyceride and low density lipoprotein cholesterin in serum; when at the dosage of 200 mg / kg-500 mg / kg, the extract has the obvious effect onlowering mouse liver fat relevant indexes of total cholesterol and triglyceride and basically has no toxic and side effect. The fu tea extract has very large application potential in the aspects of preparing lipid-lowering drugs.

The present invention discloses a blood fat three-item test card. The blood fat three-item test card comprises an upper cover (100), blood fat detection test paper (200) and a base seat (300); a sample adding hole (101) is formed in the upper cover (100); three detection holes are formed in the base seat (300), namely a first detection hole (301a), a second detection hole (301b) and a third detection hole (301c) corresponding to three modules of the blood fat detection test paper respectively; and the components of the blood fat detection test paper sequentially comprises a filter layer (201),a diffusion layer (202), a blood filter layer (203), an interception layer (204) and a reaction layer (205) from top to bottom. The blood fat test card is convenient to operate and high in anti-interference capability, and can detect total cholesterol, triglyceride and high-density lipoprotein cholesterol in human blood.

The present invention is intended to provide a method for simultaneously measuring cholesterol in low density lipoprotein and total cholesterol as test components in blood. Specifically, a method is used for simultaneously measuring cholesterol in low density lipoprotein and total cholesterol in a biological sample, whereby cholesterol in low density lipoprotein and total cholesterol in a biological sample are quantified with a single measurement.

The invention discloses application of a traditional Chinese medicine composition in preparation of drugs for preventing and treating abnormal fat metabolism diseases. The traditional Chinese medicinecomposition is prepared from herba artemisiae scopariae, radix bupleuri, radix sophorae flavescentis, radix et rhizoma rhei and folium isatidis. Through screening of a large number of experiments, the traditional Chinese medicine composition provided by the invention has a good effect of reducing the content of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) of a hyperlipidemia animal model, and has a certain effect of increasing high-density lipoprotein cholesterol (HDL-C). The clinical experiments find that the Kuhuang granules can obviously improvebiochemical indexes and imaging evaluation results of fatty liver patients. In addition, the experimental result of the drug-induced liver injury shows that the traditional Chinese medicine composition provided by the invention can significantly improve the drug-induced liver injury and has the effect of preventing the liver injury.

A Chinese medicine for treating hyperlipemia is prepared from 10 Chinese-medicinal materials including radish seed, haw, red sage root, Chuan-xiong rhizome, etc. Its preparing process is also disclosed.

Preparation of azetidinone compounds and medical use thereof are provided by the present invention. More particularly, azetidionne compounds, shown as formula (I), wherein R1, R2, R3, R4, R5 and R6 are defined in description, and preparation methods thereof are provided by the present invention. The compounds of the present invention can reduce the levels of total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) in plasma, and can be used as medicaments for reducing cholesterol in blood. Therefore the compounds of the present invention can be used to treat or prevent diseases of atherosclerosis, cacergasia of blood vessel, cardiac failure, coronary artery disease, angiocardiopathy, myocardial, angina, hyperlipoidemia and hypercholesteremia and the like. Preparation method of compounds of formula (I) and intermediate compounds are also provided by the present invention.

The present invention provides blood-lipid-lowering food. The blood-lipid-lowering food is characterized by comprising the following materials of cassia seed powder, alismatis rhizome powder, haw powder and konjac soluble dietary fibers. The blood-lipid-lowering food has an effect of lowering serum triglyceride (TG) content to 1.45 plus or minus 0.17 mmol / L and total cholesterol content to 4.55 plus or minus 0.11 mmol / L after hyperlipidemia populations with serum total cholesterol (TC) content of 6.5-7.0 mmol / L or serum triglyceride (TG) content of 2.5-3 mmol / L eating the food for 6 months.

The invention is applicable to the field of biotechnology, and particularly relates to a detection kit for S100B protein and a using method of the detection kit. The kit comprises an elisa plate coated with an anti-S100B monoclonal antibody, a horse radish peroxidase labeled anti-S100 polyclonal antibody solution for detection, a standard substance, a standard substance diluent, a sample diluent,a concentrated washing solution, a developing solution A, a developing solution B, a stop solution and a detection antibody diluent. The kit disclosed by the invention is relatively high in sensitivity, and the lowest detection limit of the kit is as low as 1pg / mL; the uniformity is good, the stability is high, the intra-batch variation coefficient is less than 10%, and the inter-batch variation coefficient is less than 5%; the specificity is good, the cross reaction between the kit and serum total bilirubin TBIL, triglyceride TG and total cholesterol TC which are easy to interfere with the test in serum is very low, and the measurement of S100B is not interfered; the clinical trial effect is good, and the single detection cost is lower than that of the same type of kit on the market.

The present invention relates to the technical field of Chinese herb preparation and a preparation method thereof, and provides a medicine used for remedying hyperlipemia and a preparation method thereof. The medicine includes the following materials according to the following weight portions: 240 to 360 portions of blackcurrant seed, 500 to 750 portions of hawthorn, 100 to 150 portions of liquorice and 0 to 144 portions of auxiliary material. The medicine remedying hyperlipemia of the invention is pure Chinese herb preparation, avoiding the side effect of foreign medicine on remedying hyperlipemia. The invention can reduce total cholesterol, triglyceride, low density lipoprotein cholesterin in quail-model serum of hyperlipemia and lighten the formation of atherosclerotic plaques. The invention has obvious efficacies on reducing total cholesterol, low density lipoprotein cholesterin and lightening the formation of atherosclerotic plaques. The invention also has the advantages of simple preparation method, safety and good reliability.

The invention belongs to a method for screening an effective hypolipidemic component in medicated edible fungus by establishing a foam cell model in cytobiology. The method comprises the following steps of: inducing a macrophage RAW264.7 by using an oxidized low-density lipoprotein (OX-LDL) to cause fatty degeneration to obtain a foam cell; combining oil red O staining and measuring the content of total cholesterol and the contents of free cholesterol and cholesterol ester in the cell to detect the effusion condition of cholesterol in the cell to achieve the effects of checking whether the macrophage has fatty degeneration and damage or not and treating the macrophage having fatty degeneration by use of medicines; and detecting and screening the effective hypolipidemic component in the edible fungus through detecting the treatment effect on the foam cell model.

The invention belongs to the technical field of solid beverages, and particularly relates to a high-protein probiotic solid beverage. The high-protein probiotic solid beverage comprises the following component raw materials in parts by weight: 65-70 parts of soybean protein isolate, 15-20 parts of whole milk powder, 15-26 parts of resistant dextrin, 15-20 parts of maltooligosaccharide powder, 10 parts of oat crisp grains, 15-25 parts of nonfat dry milk powder, 10-15 parts of whey protein and 10-15 parts of fish collagen peptide powder. The high-protein probiotic solid beverage disclosed by the invention has the beneficial effects that the soybean protein isolate is used as a main material, so that the intake of cholesterol and saturated fat is reduced, and besides, the balance of nutrient intake is also achieved; the soybean protein can prevent urinary calcium loss, promote bone health, reduce the probability of suffering from osteoporosis, and effectively reduce the content of total cholesterol and low-density lipoprotein cholesterol in blood of human bodies, so that the risk of suffering from heart diseases is reduced; and besides, original nutrition inhibiting factors namely trypsin inhibitors in soybeans are removed from the soybean protein isolate powder, so that people do not need to worry about adverse reactions of indigestion, gaseous distention and the like.

The invention provides applications of a grapefruit extract in preparation of lipid-lowering functional food, healthy food or medicines. The grapefruit extract is prepared by the following steps: performing acetone ultrasonic extraction, rotary evaporation, solid-phase extraction column passing and vacuum drying to freeze-dried powder of extracts of a grapefruit oil gland layer, an albedo layer and a mesocarp layer. The body weight of high-lipid induced fat C57BL / 6 mouse can be reduced in vivo through the grapefruit extract, the masses of the liver and adipose tissues can be obviously decreased, the triglyceride, total cholesterol and low-density lipoprotein cholesterol containing in serum can be obviously reduced, and the triglyceride and total cholesterol contained in the liver can be reduced. The grapefruit extract can be used for prevention and treatment of fat metabolism abnormality related diseases as functional food, healthy products or preventive and treatment drugs.