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Applications of grapefruit extract

A technology of extract, Huyou, applied in the field of medicine to achieve the effect of reducing the quality of liver and adipose tissue

Inactive Publication Date: 2015-04-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on whether the extract of Huyou has lipid-lowering activity in vivo, and whether the extracts from different tissue parts of Huyou have tissue-specific lipid-lowering properties.

Method used

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  • Applications of grapefruit extract
  • Applications of grapefruit extract
  • Applications of grapefruit extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation of embodiment 1 Huyou extract

[0019] Huyou fruits are manually separated to obtain the oil cell layer, white cortex layer and capsule coating layer. After being quick-frozen in liquid nitrogen, they are dried in a low-temperature vacuum, and the freeze-dried powder is obtained after grinding. Weigh 1 g of the freeze-dried powder of the oil cell layer, white cortex layer and capsular layer of grapefruit respectively, and use 20 mL of 80% acetone (solid-to-liquid ratio 1:20) to sonicate for 30 min at a frequency of 60 kHz and a power of 30 W. The extract was filtered under reduced pressure to obtain a clear extract, and this was repeated three times. The supernatants were combined, and the acetone was evaporated on a rotary evaporator to obtain an aqueous solution of grapefruit extract.

[0020] Aqueous Sep-Pak with Solid Phase Extraction (SPE) ? C18 column was used for purification (12 cc / 2 g, Waters). The purification process was divided into f...

Embodiment 2

[0021] Example 2 C57BL / 6 mouse test of Huyou extract

[0022] C57BL / 6 mice were purchased from Shanghai Slack Experimental Animal Co., Ltd., and the low-fat diet (containing 10% fat) and high-fat diet (containing 45% fat) were purchased from Jiangsu Medison Biomedical Co., Ltd. All mice were housed in a constant temperature and humidity environment with 12 h of light and 12 h of darkness. 10 mice in each group, the low-fat+clear water group is the low-fat diet group, the high-fat+clear water group is the high-fat diet-induced obesity group, and the three treatment groups of oil cell layer, white cortex and capsular layer extract of grapefruit Both are 40 mg / kg BW. All mice were free to ingest food and distilled water, the treatment group was administered by intragastric injection, and the low-fat and high-fat control groups were intragastrically administered distilled water. The test lasted for 16 weeks, with continuous administration for 6 days a week and 1 day of drug wi...

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Abstract

The invention provides applications of a grapefruit extract in preparation of lipid-lowering functional food, healthy food or medicines. The grapefruit extract is prepared by the following steps: performing acetone ultrasonic extraction, rotary evaporation, solid-phase extraction column passing and vacuum drying to freeze-dried powder of extracts of a grapefruit oil gland layer, an albedo layer and a mesocarp layer. The body weight of high-lipid induced fat C57BL / 6 mouse can be reduced in vivo through the grapefruit extract, the masses of the liver and adipose tissues can be obviously decreased, the triglyceride, total cholesterol and low-density lipoprotein cholesterol containing in serum can be obviously reduced, and the triglyceride and total cholesterol contained in the liver can be reduced. The grapefruit extract can be used for prevention and treatment of fat metabolism abnormality related diseases as functional food, healthy products or preventive and treatment drugs.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the application of a natural-sourced grapefruit extract in the preparation of lipid-lowering functional food, health products or medicines, in particular to a preparation method of the grapefruit extract and the application of the grapefruit extract to reduce lipids in mice. Background technique [0002] In recent decades, obesity has become a major public health problem worldwide. Obesity can increase the risk of chronic diseases such as type 2 diabetes, coronary heart disease, and hypertension. Obesity is a metabolic syndrome induced by an imbalance between energy intake and metabolic expenditure, manifested as fat accumulation in adipose tissue, leading to an increase in the number and size of adipocytes. At present, the methods used for obesity treatment mainly include dieting and taking weight-loss drugs, but there are many limiting factors, such as side effects and easy rebound. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A23L1/212A23L1/29A61P3/06A23L19/00A23L33/00
Inventor 李鲜贾胜孙崇德陈昆松
Owner ZHEJIANG UNIV
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