The invention discloses an essence liquid containing tuber melanosporum extract and a preparation method thereof. The essence liquid comprises a component A, a component B and water. The component A comprises butanediol, diglycerin, sodium polyacrylate, xanthan gum, modified potato starch, betaine and disodium ethylenediaminetetraacetate. The component B comprises mulberry leaf extract, tuber melanosporum extract, a lactobacillus/soybean milk fermentation product filtrate, purslane extract, bifida ferment lysate filtrate, grapefruit seed extract, bifida ferment lysate, Aloe vera leaf polysaccharide, beta-glucan, 1, 2-pentanediol, 1, 2-hexanediol, p-hydroxyacetophenone, cladosiphon novae-caledoniae extract, hydrolyzed rice bran extract, a rice fermentation product filtrate, soluble proteoglycan, aloe extract, licorice root extract, radix angelicae sinensis, salvia miltiorrhiza flower/leaf/root extract, cornus officinalis fruit extract, wolfberry root extract, tremella polysaccharide, hyaluronic acid, and the balance of deionized water.