67 results about "Punicalagin" patented technology

The invention belongs to the field of a separation method, in particular to a method for simultaneously extracting punicalagin and ellagic acid from pomegranate bark. The invention solves the technical problem of effectively utilizing the pomegranate bark resources for simultaneously extracting the punicalagin and the ellagic acid. Concretely, the method of the invention comprises the following steps: using the pomegranate bark as raw materials; firstly, using 25 to 35 percent ethanol to extract the pomegranate bark; using macroporous resin for absorption after an extracting solution is concentrated; concentrating eluent to obtain punicalagin extracts after the desorption; then, carrying out diacolation extraction on the pomegranate bark after the extraction of the punicalagin by 70 to 90 percent ethanol; adding NaOH for hydrolysis after the extracting solution is concentrated and the ethanol is removed; adding HCl after the hydrolysis for generating sediments; and carrying out the separation to obtain the sediments which are ellagic acid extracts. The method is adopted for realizing the goal of extracting two active ingredients from one batch of pomegranate bark. Both of the yield and the purity of the obtained products are high, the existing market selling requirement can be met, and the pomegranate bark resources can be fully and reasonably utilized.

The invention relates to a method for preparing a punicalagin molecularly imprinted polymer monolithic column by utilizing a in-situ polymerization method, and the method is characterized in that pre-polymerized mixed solution is directly injected inside a stainless steel pipe column to prepare continuous bar-shaped polymer, and a cross-linked polymer material with fixed size and fixed shape of holes and regularly-arranged functional groups and identifying template molecules, i.e. molecularly imprinted polymer is obtained. The molecularly imprinted polymer monolithic column prepared through the method has advantages of good imprinting effect and good permeability, and the imprinted factor reaches 13.1; and meanwhile, the method is simple in preparation process, the internal structure of the molecularly imprinted polymer monolithic column is uniform, recurrence is good, the complicated column loading procedures can be avoided, and the molecularly imprinted polymer monolithic column can be directly used for analysis.

The invention discloses a punica granatum peel antitumor polyphenol effective part, which is obtained by subjecting a punica granatum peel ethanol extract to macroporous resin separation and purification. Specifically, the total weight of polyphenol components accounts for 50-80% of the total weight of the effective part, and the weights of ellagic acid, gallic acid and Punicalagin respectively account for 10-25%, 0.80-1.2% and 35-53% of the effective part. The punica granatum peel antitumor polyphenol effective part makes full use of the characteristic that punica granatum peel resources and the active component content meet the requirements for adjunctive treatment and/or prevention of tumors. The invention also discloses a preparation method of the punica granatum peel antitumor polyphenol effective part and application of the punica granatum peel antitumor polyphenol effective part in preparing pharmaceutical preparations for adjunctive treatment and/or prevention of tumors.

The invention provides a method for extracting punicalagins from granatum, comprising the following steps: smashing granatum; adding 5-15 times of 30-60% of alcohol; carrying out ultrasonic extraction for 1-3 times; filtering to obtain the extract; adding an ultrafiltration membrane for ultrafiltration; adding nanofiltration membrane for concentration; adding charcoal column into the concentrated solution for adsorbing; resolving by 80-90% of alcohol solution; concentrating the resolving solution by the nanofiltration membrane; adding ethyl acetate into the concentrated solution to extract for 3-5 times; decompressing and recovering the ethyl acetate; and vacuum drying the concentrated solution to obtain the product. In the process, an ultrasonic extraction and membrane separation manner is adopted; the ultrasonic extraction and the membrane separation are performed at the normal temperature; the process has low energy consumption, avoids high temperature decomposition of the product, has simple process operation process, and is suitable for industrialization production.

This invention describes a simple, rapid and cost-effective method to isolate bulk quantities of relatively pure and enriched anthocyanidins and other plant bioactives. The method is based on the principle of solubility. Some bioactives (anthocyanidins) were extracted in an aqueous solvent, transferred to a non-aqueous solvent and finally insolubilized by adding a miscible solvent in which the bioactive was insoluble. Thus, anthocyanidins were isolated from anthocyanin-enriched berries or non-enriched, dark-colored fruits, vegetables and grains by extraction of anthocyanins, acid hydrolysis, and extraction of the resulting anthocyanidins, followed by their insolubilization (precipitation). Some bioactives (hydrophobic and hydrophilic) were extracted in a solvent with high solubility and then directly insolubilized by adding a miscible solvent in which the bioactive was insoluble, for example, withaferin A from enriched Withania somnifera and punicalagins from enriched punica extract.

The invention discloses a method for separating and purifying punicalagin in pomegranate peel. The method includes the steps of 1, carrying out crude extraction, namely grinding the pomegranate peel to obtain pomegranate peel powder, and extracting the pomegranate peel powder by extraction ethyl alcohol so as to obtain crude extraction liquid of the punicalagin; 2, adsorbing, namely taking the crude extraction liquid of the punicalagin as adsorption liquid, and subjecting the crude extraction liquid of the punicalagin to resin adsorption by macroporous resin so as to obtain adsorption resin; 3, desorbing, namely subjecting the adsorbent resin to desorption by the ethyl alcohol and collecting eluent; 4, purifying, namely concentrating the eluent and purifying the concentrated eluent by means of high-speed counter-current chromatography so as to obtain a purified punicalagin product. The method for separating and purifying the punicalagin in the pomegranate peel has the advantages of simple steps, low industrialized operation cost, high punicalagin extraction ratio, excellent purification effect and capability of enabling the purity of the punicalagin in the purified punicalagin product to be above 90%.

The invention provides a method for simultaneously separating and purifying punicalagin and gallic acid from pomegranate rind. The method comprises the following steps: raw material pretreatment, extraction, concentration, water precipitation decolorizing, resin adsorption, resin desorbing, desorbed solution extraction, and crystallization. The method realizes the simultaneous separation and purification of the punicalagin and the gallic acid from the pomegranate rind, the content of the separated and purified punicalagin is more than 98%, the content of gallic acid is more than 96%, the recovery rate of the punicalagin is more than 71%, and the recovery rate of the gallic acid is more than 68%.

A topical cosmetic composition for increasing skin whitening or lightening is described. The composition includes a pomegranate extract in an amount effective to reduce melanin composition. The pomegranate extract may be standardized to about 20% punicalagin. The composition may also include an extract from the genus larix, such as Larix sibirica. The larix sibirica may be standardized to 80% taxifolin. A method for using the compositions is described.

The invention relates to a preparation method for a pomegranate rind effective component having strong anti-oxidation and staphylococcus aureus inhibitory activity. The method uses a macroporous resinpurified product of a pomegranate rind extract as a raw material and uses a non-linear reversed-phase chromatography technology to obtain the pomegranate rind effective component having a punicalagincontent as high as 60.6% through purification, and the pomegranate rind effective component having the strong anti-oxidation and staphylococcus aureus inhibitory activity is obtained by researching the content, recovery rate and preparation efficiency of punicalagin in the pomegranate rind effective component obtained under different sample loading amounts of an analytical column. The method hasa stable process, is very suitable for further industrial production, and lays a solid foundation for the development of novel drugs based on the pomegranate rind effective component as a bulk drug.

The invention relates to the technical field of biological engineering, in particular to a preparation method of punicalagin molecular imprinted polymer microspheres. The method comprises the following steps of: dissolving a template molecule of punicalagin, a function monomer of acrylamide, a cross-linking agent of ethylene glycol dimethacrylate and an initiator of azodiisobutyronitrile into a pore-foaming agent of acetonitrile, ultrasonically dispersing the components at the normal temperature, and introducing nitrogen to seal, wherein the molar ratio of the template molecule and the function monomer is 1:5-20, the molar ratio of the function monomer and the cross-linking agent is 1:5, the volume rate of the cross-linking agent and the pore-foaming agent is 20:1000, and the mass ratio of the dosage sum of the initiator and the function monomer as well as the crossing agent is 2:100; stirring the mixed solution for 24h at 60 DEG C to carry out polymerization reaction; centrifugally separating the mixed solution and drying for 24h at 50 DEG C, and removing the template molecule by means of a Soxhlet extraction method; and drying. In the invention, the punicalagin can be selectively absorbed with high absorption quantity, and the maximum absorption quantity is up to 35299ug/g, thereby the process is simple and the cost is low.

The invention discloses application of pomegranate in preparing a medicament for treating or preventing hepatitis B virus (HBV) infection. The application is implemented through the following steps: detecting the cytotoxicity of each pomegranate sourced medicament to HepG2.117, wherein the CC50 of punicalagin, gallic acid and punicalin is sequentially more than 500mu M, 334mu M and 218.86mu M; and then detecting the effects of the punicalagin, ellagic acid, gallic acid and punicalin to HBeAg and HBsAg, and the effect of punicalagin to DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) of virus. Results indicate that the four compounds can be used for inhibiting HBeAg and HBsAg, wherein the punicalagin can be used for remarkably reducing the cccDNA (covalently closed circular DNA). The punicalagin can be used for remarkably reducing the contents of HBeAg and cccDNA in an HepG2.2.15 cell, and does not have effect to the total HBV DNA in the cell, which indicates that the punicalagin takes the cccDNA as a target to directly clear the cccDNA in a liver cell. Currently, the anti-HBV infection medicament in clinical treatment cannot directly act on the cccDNA, and cannot completely remove the virus. Experiment results disclose that the medicament has the prospect of being developed into anti-HBV infection medicaments.

A pharmaceutical composition with an active pharmaceutical ingredient including a pomegranate fruit polyphenol extract. The pomegranate fruit polyphenol extract includes at least about 3% combined punicalagin A and punicalagin B by weight, less than about 5% ellagic acid and their derivatives by weight, and less than about 1% anthocyanins by weight.

The invention relates to a method for purifying and preparing punicalagin on the basis of isomerization characteristics. According to the method, a pomegranate peel extract is used as a raw material,and impurities contained in the pomegranate peel extract are avoided on the basis of the structural characteristic that the pomegranate peel extract has two isomers capable of being mutually converted; a large amount of punicalagin with the purity higher than 98% is obtained from the complex pomegranate peel extract by utilizing pilot-scale preparative liquid chromatography. The method is very simple and convenient, the purity of the obtained punicalagin is high, the preparation amount is large, and the method has a high reference value for purification preparation of compounds with the isomerization characteristics.

The invention relates to a method for preparing high-purity punicalagin crystal powder. The method disclosed by the invention comprises the steps: firstly, preparing TiO2 compound copolymer microspheres which have a function of separating a polar organic compound efficiently and effectively separating an organic compound with polyhydroxyl in a natural product; then initially purifying a punicalagin crude product with a mass fraction as 20 to 40% to 75 to 80% by utilizing the TiO2 compound copolymer microspheres as filler and utilizing an ordinary-pressure chromatography, wherein processing flux is large and consumed time is short; finally, utilizing the certain amount of methyl alcohol, acetone, diethyl ether and ethyl acetate mixed solution to dissolve a purified punicalagin sample, adding trichloromethane, centrifuging to take supernatant, putting in 4 DEG C environment and crystallizing to obtain punicalagin crystal with a purity as 97 to 98%. According to the method disclosed by the invention, the crystallizing method is utilized for the first time to obtain high-purity punicalagin crystal powder; the technology is simple, organic solvent consumption is small, sample processingflux is large, and consumed time is short; furthermore, required equipment cost is low, the problem of industrially preparing the high-purity punicalagin crystal is solved, and the method can be industrially popularized.

The invention relates to application and medical application of punicalagin as a PTP1B inhibitor and further relates to application of punicalagin in preparation of medicine for preventing or treating diseases relevant to protein-tyrosine-phosphatase 1B (PTP1B). Punicalagin serves as a natural active compound in plants and has high success probability in the later research and development process of the medicine.

The invention discloses application of punicalagin in inhibiting growth of multi-drug resistant klebsiella pneumoniae. Punicalagin has a relatively good in-vitro killing effect on multi-drug resistantklebsiella pneumonia and can inhibit the growth of multi-drug resistant klebsiella pneumoniae, and the minimum bactericidal concentration is 3.6 mg/mL and the minimum bacteriostatic concentration is1.8 mg/mL. The invention provides the inhibiting effect of punicalagin on multi-drug resistant klebsiella pneumoniae, and the punicalagin has wide application value in the fields of medicine and the like.

The invention belongs to the technical field of medicines, and discloses pomegranate rind tannin and application of punicalagin and granatin serving as effective components of the pomegranate rind tannin in preparation of medicines for treating ulcerative colitis. After the pomegranate rind tannin is used for treating mouse ulcerative colitis induced by dextran sulfate sodium salt, the body weight, the disease activity index, the colorectum length, the colorectum mucous membrane form and the goblet cell number are improved to a certain extent compared with a model group; therefore, the pomegranate rind tannin has a certain treatment effect on ulcerative colitis. The effective components punicalagin and granatin in the pomegranate rind tannin are independently or jointly administered, so that the pomegranate rind tannin has a very good effect on ulcerative colitis, and drug combination is optimal.

The present invention discloses feature-beautifying mineral water. The feature-beautifying mineral water comprises the following components in parts by weight: 1-2 parts of zinc gluconate, 1-3 parts of tea polyphenol, 2-4 parts of organic selenium, 0.8-1.0 part of a grape seed extract, 6-10 parts of a pomegranate extracting solution, 10-16 parts of a fresh flower extracting solution, and 100-500 parts of mineral water. The pomegranate extracting solution is rich in pomegranate polyphenols, punicalagin, ursolic acid, various amino acids and trace elements, can be preserved for a long time, andalso has high oxidation resistance and taste close to fruit flavor. The fresh flower extracting solution is rich in various amino acids, vitamins, etc., has functions of quenching thirst, nourishing the skin, whitening the skin, eliminating fatigue and enhancing human body resistance, and is suitable for drinking in the four seasons. Through the reasonable blending, the feature-beautifying mineralwater has the special feature-beautifying effect, is greatly favored by white-collar workers, richer in nutrients than ordinary mineral water, prominent in functions, healthier than juice, milk tea and other drink, especially suitable for young people who like to drink water, and faint in sourness and floral fragrance, can greatly increase the desire to drink water, and has very broad market prospects.

The invention discloses a composition for inhibiting absorption of sugar and fat. The composition for inhibiting absorption of sugar and fat comprises the following components in percentage by weight: less than 94% of green tea extract containing tea polyphenol, 3-20% of rose extract containing polyphenol and more than 3% of pomegranate fruit extract containing polyphenol and punicalagin. The composition for inhibiting sugar and fat absorption prepared by the invention can realize dual inhibition on alpha-amylase and alpha-glucosidase, and can inhibit the activity of lipase at the same time, so that the effect of reducing fat is further achieved. The composition can remarkably improve the bitter taste of the green tea extract independently containing tea polyphenol, improve the taking experience of eaters and enhance the long-term taking willingness of the eaters. The composition is clear in component and effect, wide in application range, free of toxic and side effects, easy to prepare and operate, low in cost, wide in application dosage form and convenient to produce and apply.

The invention relates to the technical field of punicalagin preparation, in particular to a punicalagin purification method and application of punicalagin in preparation of an alpha-glucosidase inhibitor. According to the invention, D101 macroporous resin and a C18 semi-preparative chromatographic column are adopted to carry out chromatographic column gradient elution on a punicalagin extract twice; the eluents of the chromatographic column gradient elution twice are set as methanol and water; the gradient elution procedure during each chromatographic column purification is specifically controlled; the interference of coexisting impurities is avoided; and the purity of the extracted punicalagin is improved. Results of the embodiment show that the purity of punicalagin in the punicalagin purified product obtained by the purification method provided by the invention is greater than 92%.