Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting punicalagin from pomegranate bark

A technology of punicalagin and pomegranate peel, which is applied in the field of preparation of plant extracts, can solve the problems of being unsuitable for the promotion of industrial production, complex steps of the extraction method, cumbersome operation, etc., and achieve high product yield, short time and low dosage Effect

Active Publication Date: 2013-12-25
三原金瑞生物工程有限公司
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of punicalagin in the extract obtained in Scheme 1 is low, only 40%, and the quality is low; although the content of punicalagin in Scheme 2 has reached more than 98%, the extraction method is complicated and requires two column chromatography , it takes a long time, the operation is cumbersome, and it is not suitable for the promotion of industrial production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Extract punicalagin from pomegranate peel, the steps are as follows:

[0021] 1) Using pomegranate peel as raw material, add 9 times its weight in 20% ethanol solution to reflux and extract twice, each time for 2 hours, combine the extracts, concentrate the extracts to 2 times the weight of the raw material, pass through a container filled with macroporous resin The chromatographic column first washes the macroporous resin with water, then elutes with 20% ethanol solution, collects the eluate of the ethanol solution, concentrates, and dries to obtain a crude extract;

[0022] Wherein, the model of the macroporous resin is D101, the upper column flow rate is 0.8BV / h; the washing volume is 1.2BV, the washing flow rate is 0.3BV / h, and the ethanol solution elution flow rate is 0.5BV / h;

[0023] 2) Use the fluid composed of dimethyl ether, liquid ammonia and dimethylamine in a weight ratio of 8:3:2 as the extractant to perform subcritical extraction on the crude extract, and...

Embodiment 2

[0025] Extract punicalagin from pomegranate peel, the steps are as follows:

[0026] 1) Using pomegranate peel as raw material, add 8 times its weight in 20% ethanol solution to reflux and extract for 3 times, each time for 1 hour, combine the extracts, concentrate the extracts to 2 times the weight of the raw materials, pass through a container filled with macroporous resin The chromatographic column first washes the macroporous resin with water, then elutes with 30% ethanol solution, collects the eluate of the ethanol solution, concentrates, and dries to obtain a crude extract;

[0027] Wherein, the model of the macroporous resin is D101, the upper column flow rate is 0.5BV / h; the washing volume is 1.0BV, the washing flow rate is 0.25BV / h, and the ethanol solution elution flow rate is 0.4BV / h;

[0028] 2) Use the fluid composed of dimethyl ether, liquid ammonia and dimethylamine in a weight ratio of 8:3:1 as the extractant to perform subcritical extraction on the crude extra...

Embodiment 3

[0030] Extract punicalagin from pomegranate peel, the steps are as follows:

[0031] 1) Using pomegranate peel as raw material, add 9 times its weight in 30% ethanol solution to reflux extraction once, each time for 2 hours, concentrate the extract to 2.5 times the weight of the raw material, pass through a chromatography column filled with macroporous resin, Washing the macroporous resin first, then eluting with 30% ethanol solution, collecting the eluate from the ethanol solution, concentrating, and drying to obtain a crude extract;

[0032] Wherein, the model of the macroporous resin is D101, the upper column flow rate is 1.0BV / h; the washing volume is 0.6BV, the washing flow rate is 0.3BV / h, and the ethanol solution elution flow rate is 0.6BV / h;

[0033] 2) Use the fluid composed of dimethyl ether, liquid ammonia and dimethylamine in a weight ratio of 7:4:3 as the extractant to perform subcritical extraction on the crude extract, and the injection amount of the extractant ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting punicalagin from pomegranate bark. The method includes the following steps that 1) the pomegranate bark is used as a raw material, reflux extraction is conducted by using a 10%-30% ethanol solution, an extracting solution is concentrated, by the aid of macroporous resin, the macroporous resin is washed, then a 20%-30% ethanol solution is used for conducting elution, the eluant is collected, concentrated and dried, and then a crude extract is obtained; 2) the fluid which is formed by dimethyl ether, liquid ammonia and dimethylamine according to the weight ratio of 7-9 : 2-4 : 1-3 is used as an extraction agent, subcritical extraction is conducted on the crude extract, the injection rate of the extraction agent is once or twice of the weight of the crude extract, the extraction temperature is 28-31 DEG C, the extraction pressure is 1.2-1.6MPa, the extraction time is 25-45min, and after the self-volatilized extraction agent is recycled, a finished product is obtained. According to the method for extracting the punicalagin from the pomegranate bark, when reflux extraction and macroporous resin washing are carried out, a low concentration ethanol solution is selected, the punicalagin can be better extracted, afterwards, further subcritical extraction is conducted, the time is short, operation is achieved at one step, product yield and quality are high, and therefore the method is more suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method of a plant extract, in particular to a method for extracting punicalagin from pomegranate peel. Background technique [0002] Punicalagin is the skin extract of pomegranate (Punica granatum L.). After being absorbed by the human body, punicalagin can be decomposed into ellagic acid under the action of human enzymes. It has excellent antioxidant properties and is mostly used in the fields of food and cosmetics. At the same time, ellagic acid has the effect of anti-mutation and inhibiting the proliferation of cancer cells. It is mostly used to prevent and treat colon cancer, esophageal cancer, liver cancer, lung cancer and skin tumors. Compared with ellagic acid, punicalagin has the advantages of good water solubility and easy absorption by the human body, so it is of great significance to extract high-purity punicalagin from pomegranate peel. [0003] In the prior art, for example, CN100589813C discloses a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/08C07H1/08
Inventor 蒋元勇文继承朱昂辉雍小军易华李丽金
Owner 三原金瑞生物工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com