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Method for preparing punicalagin and ellagic acid from pomegranate rind

A technology of punicalagin and pomegranate peel, which is applied in the field of plant extraction and can solve the problems of unseen methods and reports of punicalagin and ellagic acid

Active Publication Date: 2012-05-23
西安天美生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Through patent retrieval and domestic and foreign papers review, there is no method and report on the simultaneous preparation of punicalagin and ellagic acid using the same batch of pomegranate peel raw materials

Method used

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Embodiment 1

[0031] making process:

[0032] The invention discloses a preparation method for extracting ellagic acid from the production wastewater that has extracted pomegranate peel polyphenols or punicalagin.

[0033] Its concrete preparation steps are as follows:

[0034] (1) Raw material preparation: take fresh pomegranate peel or dried pomegranate peel as raw material, wash and crush;

[0035] (2) Extraction: at 40°C, soak and extract with 0.4% hydrochloric acid solution for 3 hours, extract twice, and the ratio of solid to liquid is: 1:30;

[0036] (3) filter: filter, merge filtrate, abandon raw material slag;

[0037] (4) Concentration: low-temperature vacuum concentration, concentrated to: the amount of paste output: the amount of raw materials = 10:1;

[0038] (5) Extraction 1: organic solvent: concentrated paste = 1: 2, forced circulation extraction for 1 hour, and separate the water phase;

[0039] (6) Extraction 2: Add chloroform to the aqueous phase obtained in the previ...

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Abstract

The invention discloses a method for preparing punicalagin and ellagic acid from pomegranate rind, comprising the following steps of: preparing materials; extracting; filtering; concentrating; extracting 4 times; concentrating; drying; acidifying; hydrolyzing; purifying; and finally, obtaining punicalagin and ellagic acid, wherein the punicalagin has high purity above 40 percent and yield of 11:1, and the ellagic acid has high purity above 90 percent and yield of 21:1. The method can sufficiently use production waste liquid obtained after the extraction of pomegranate rind total phenolics andthe punicalagin as a raw material to prepare the high-purity ellagic acid and has positive actions for sufficiently using natural product resources, increasing added values of farm products, lightening environmental protection pressure and reducing industrialized production cost.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for preparing punicalagin and ellagic acid from pomegranate peel. Background technique [0002] Pomegranate (Punica granatum L) belongs to the pomegranate family Pomegranate genus deciduous shrubs or small trees. It is distributed in subtropical and temperate regions. Pomegranates are planted in various regions in the north and south of my country. There are five most influential pomegranate producing areas in China: Lintong, Shaanxi , Zaozhuang, Shandong, Huaiyuan, Anhui, Huili, Sichuan, and Mengzi, Yunnan. [0003] Pomegranate peel is the dry peel of pomegranate, which is irregular in flake or scoop shape, with a thickness of 1.5-3mm. The outer surface is reddish-brown, brownish-yellow or dark brown, slightly shiny, rough, with many verrucous protrusions. Some have protruding tubular persistent calyx and thick and short fruiting stems or scars. The inner sur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H13/08C07H1/08C07D493/06
Inventor 洪光宇张小燕
Owner 西安天美生物科技股份有限公司
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