Method for extracting punicalagins from granatum

A technology of punicalagin and its extraction method, which is applied in the field of ultrasonic extraction and membrane separation to extract punicalagin, which can solve the problems of high energy consumption and achieve the effects of low energy consumption, simple process flow and short cycle

Inactive Publication Date: 2011-01-26
NANJING ZELANG MEDICAL TECH
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  • Method for extracting punicalagins from granatum

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Embodiment 1

[0027] Grind pomegranate peel, take 2kg, put it into the ultrasonic extraction tank for testing, take 20kg of 60% ethanol for ultrasonic extraction for 1 hour, the ultrasonic frequency is not lower than 20KHz, filter the extract, then add 16kg, 10kg of 60% ethanol for extraction twice, each time 1 hours, combined three extracts, filtered and added hollow composite ultrafiltration membrane ultrafiltration system with molecular weight cut off 10000 for ultrafiltration, appropriate amount of water for dialysis, and then added hollow composite nanofiltration membrane system nanofiltration with molecular weight cut off 800 for permeate, and appropriate amount of water Dialyzed to obtain a concentrated solution. Add the concentrated solution to a short and thick activated carbon column (diameter-to-height ratio 1:5) for adsorption. After the adsorption is completed, analyze it with 90% ethanol solution until there is no red color and become lighter. , and an appropriate amount of wa...

Embodiment 2

[0029] Pulverize pomegranate peel, take 2kg, put it into the ultrasonic extraction tank for testing, take 20kg of 30% ethanol for ultrasonic extraction for 1 hour, the ultrasonic frequency is not lower than 20KHz, filter the extract, then add 16kg and 10kg of 30% ethanol for extraction twice, each time 1 hour, combined three extracts, filtered and added a hollow composite ultrafiltration membrane ultrafiltration system with a molecular weight cut-off of 6000 for ultrafiltration, an appropriate amount of water for dialysis, and the permeate was added for nanofiltration with a hollow composite nanofiltration membrane system with a molecular weight cut-off of 700, and an appropriate amount of water Dialyzed to obtain a concentrated solution. Add the concentrated solution to a short and thick activated carbon column (diameter-to-height ratio 1:8) for adsorption. After the adsorption is completed, analyze it with 80% ethanol solution until there is no red color and become lighter. ...

Embodiment 3

[0031] Grind pomegranate peel, take 5kg, put it into a medium-sized ultrasonic extraction tank, take 75kg of 50% ethanol for ultrasonic extraction for 1 hour, the ultrasonic frequency is not lower than 20KHz, filter the extract, add 75kg of 50% ethanol for extraction once, combine the extract, filter and add Ultrafiltration with a hollow composite ultrafiltration membrane ultrafiltration system with a molecular weight cut-off of 10,000, dialysis with an appropriate amount of water, and adding the permeate to the hollow composite nanofiltration membrane system with a molecular weight cutoff of 700 for nanofiltration, and dialysis with an appropriate amount of water to obtain a concentrated solution. Add the concentrated solution to a short and thick activated carbon column (diameter-to-height ratio 1:6) for adsorption. After the adsorption is completed, analyze it with 85% ethanol solution until there is no red color and become lighter. , and an appropriate amount of water for d...

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Abstract

The invention provides a method for extracting punicalagins from granatum, comprising the following steps: smashing granatum; adding 5-15 times of 30-60% of alcohol; carrying out ultrasonic extraction for 1-3 times; filtering to obtain the extract; adding an ultrafiltration membrane for ultrafiltration; adding nanofiltration membrane for concentration; adding charcoal column into the concentrated solution for adsorbing; resolving by 80-90% of alcohol solution; concentrating the resolving solution by the nanofiltration membrane; adding ethyl acetate into the concentrated solution to extract for 3-5 times; decompressing and recovering the ethyl acetate; and vacuum drying the concentrated solution to obtain the product. In the process, an ultrasonic extraction and membrane separation manner is adopted; the ultrasonic extraction and the membrane separation are performed at the normal temperature; the process has low energy consumption, avoids high temperature decomposition of the product, has simple process operation process, and is suitable for industrialization production.

Description

Technical field: [0001] The invention relates to a method for extracting punicalagin from pomegranate peel, in particular to a method for extracting punicalagin by ultrasonic extraction and membrane separation. Background technique: [0002] Picalagin is a tannin substance, which is the main component of pomegranate peel. The 2005 edition of "Chinese Pharmacopoeia" stipulates that the tanning in pomegranate peel should not be less than 10%. "RP-HPLC method for the determination of punicalagin in pomegranate peel" published by Li Haixia et al. In the literature, the content of punicalagin in pomegranate peel was measured by high performance liquid chromatography up to 10%. [0003] Pomegranate peel Pomegranate peel is the peel of pomegranate, a plant of the pomegranate family, which is sour, astringent, and warm in nature. Return large intestine meridian, astringent intestines for antidiarrheal, hemostasis, deworming. For chronic diarrhea, chronic dysentery, blood in the st...

Claims

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Application Information

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IPC IPC(8): C07H13/08C07H1/08
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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