Method for simultaneously extracting punicalagin and ellagic acid from pomegranate bark

Abstract

The invention belongs to the field of a separation method, in particular to a method for simultaneously extracting punicalagin and ellagic acid from pomegranate bark. The invention solves the technical problem of effectively utilizing the pomegranate bark resources for simultaneously extracting the punicalagin and the ellagic acid. Concretely, the method of the invention comprises the following steps: using the pomegranate bark as raw materials; firstly, using 25 to 35 percent ethanol to extract the pomegranate bark; using macroporous resin for absorption after an extracting solution is concentrated; concentrating eluent to obtain punicalagin extracts after the desorption; then, carrying out diacolation extraction on the pomegranate bark after the extraction of the punicalagin by 70 to 90 percent ethanol; adding NaOH for hydrolysis after the extracting solution is concentrated and the ethanol is removed; adding HCl after the hydrolysis for generating sediments; and carrying out the separation to obtain the sediments which are ellagic acid extracts. The method is adopted for realizing the goal of extracting two active ingredients from one batch of pomegranate bark. Both of the yield and the purity of the obtained products are high, the existing market selling requirement can be met, and the pomegranate bark resources can be fully and reasonably utilized.

Description

technical field [0001] The invention belongs to the field of separation methods, in particular to a method for simultaneously extracting punicalagin and ellagic acid from pomegranate peel. Background technique [0002] Pomegranate is a deciduous shrub or small tree of the pomegranate family Pomegranate. In traditional Chinese medicine, pomegranate skin has the functions of astringent intestines, diarrhea, hemostasis, and insect repellent. Abdominal pain. [0003] Modern studies have found that pomegranate peel extract (Pomegranate Hull P.E.) has excellent antioxidant properties, and also has certain antibacterial and anticancer effects, (Jurenka JS, Therapeutic applications of pomegranate——Punica granatum L, Altern Med Rev.2008jun; 13( 2): 128-144.). Therefore, pomegranate peel extract has been widely used in health food and cosmetics. The active ingredients contained in the pomegranate peel extract are mainly ellagic acid (Ellagic acid) and punicalagin (Punicalagin), the...

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Problems solved by technology

However, the raw material used in this method is gallnut, and the obtained ellagic acid has low quality, poor appearance and color, and the preparation method has many steps, long cycle, high production cost, and is not easy to be put into large-scale production.

[0011] In view of the great difference in the physical and chemical properties of ellagic acid and punicalagin, the production process of the above-mentioned pomegranate peel extract is to extract ellagic acid or punicalagin from the pomegranate peel alone, so that when a certain component is extracted, other components Lost, resulting in waste of resources, not fully and rationally utilized

At present, there is no report on the simultaneous extraction of punicalagin and ellagic acid from pomegranate peel

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