Method for extracting punicalagin from pomegranate peel

A technology of punicalagin and pomegranate peel, which is applied in the field of extracting punicalagin, can solve the problems of unsuitable industrial production promotion, complex extraction method steps, cumbersome operation, etc., to increase process time and cost, increase content and extraction efficiency, The effect of high content

Inactive Publication Date: 2015-02-04
桂林三宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of punicalagin in the extract obtained in Scheme 1 is low, only 40%, and the quality is low; although the content of punicalagin in Scheme 2 has reached more than 98%, the extraction method is complicated and requires two column chromatography , it takes a long time, the operation is cumbersome, and it is not suitable for the promotion of industrial production

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  • Method for extracting punicalagin from pomegranate peel

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Embodiment 1

[0022] A method for extracting ellagic acid from pomegranate peel, specifically comprising the steps of:

[0023] 1) Raw material processing: pulverize 100 kg of dried pomegranate peel;

[0024] 2) High-pressure micro-jet superfine pulverization treatment: Add 800kg of pure water to the pulverized pomegranate peel, process it with a high-speed shear emulsifier for 10 minutes at a speed of 12000r / min, and then use high-pressure micro-jet superfine pulverization equipment for processing at 25°C , the treatment pressure is 100MPa, and the number of treatments is 3 times to obtain a paste slurry;

[0025] 3) High-pressure extraction: Put the paste slurry obtained in step 1) into a high-pressure tank, add purified water of the same weight, and pressurize to 4kgf / cm 2 , make the pressure uniform, and keep it for 10 minutes, return to normal pressure, and obtain the extract;

[0026] 4) Collect the extract obtained after high-pressure extraction, centrifuge, and perform D101 resin ...

Embodiment 2

[0028] A method for extracting ellagic acid from pomegranate peel, specifically comprising the steps of:

[0029] 1) Raw material processing: crush 100kg of fresh pomegranate peel;

[0030] 2) High-pressure micro-jet micro-grinding treatment: Add 1,000kg of pure water to the crushed pomegranate peel, process it with a high-speed shear emulsifier for 15 minutes at a speed of 10,000r / min, and then use high-pressure micro-jet ultra-fine pulverization equipment at 25°C Processing, the processing pressure is 80MPa, and the number of processing is 4 times to obtain a paste slurry;

[0031] 3) High-pressure extraction: Put the paste slurry obtained in step 1) into a high-pressure tank, add purified water of the same weight, and pressurize to 4kgf / cm 2 , to make the pressure uniform, and keep it for 12 minutes, return to normal pressure, and obtain the extract;

[0032] 4) Collect the extract obtained after high-pressure extraction, centrifuge, and perform D102 resin column chromato...

Embodiment 3

[0034] 1) Raw material processing: crush 100kg of fresh and dried pomegranate peels;

[0035] 2) High-pressure micro-jet superfine pulverization treatment: Add 900 kg of pure water to the crushed pomegranate peel, process it with a high-speed shear emulsifier for 20 minutes at a speed of 8000 r / min, and then use high-pressure micro-jet superfine pulverization equipment at 25 ° C. Processing, the processing pressure is 120MPa, and the number of processing is 2 times to obtain a paste slurry;

[0036] 3) High-pressure extraction: Put the paste slurry obtained in step 1) into a high-pressure tank, add purified water of the same weight, and pressurize to 4kgf / cm 2 , to make the pressure uniform, and keep it for 15 minutes, return to normal pressure, and obtain the extract;

[0037] 4) Collect the extract obtained after high-pressure extraction, centrifuge, and perform AB-8 resin column chromatography on the supernatant, then elute with 50-60% ethanol solution, concentrate and spr...

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Abstract

The invention discloses a method for extracting punicalagin from pomegranate peel. The method includes following steps: treatment of raw materials, high-pressure microjet superfine grinding treatment, high pressure extraction, collection of extracts obtained after the extraction, centrifugation, nonpolar resin column chromatography to a supernate, elution through an ethanol solution with a concentration being 50-60%, concentration and a spray drying process to obtain the punicalagin. The method is high in the content of the punicalagin, is high in extraction ratio, is simple in processes, is sutiable for production and is low in production cost.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting punicalagin from pomegranate peel. Background technique [0002] Pomegranate (Punica granatum L) belongs to the pomegranate family Pomegranate genus deciduous shrubs or small trees. It is distributed in subtropical and temperate regions. Pomegranates are planted in various regions in the north and south of my country. There are five most influential pomegranate producing areas in China: Lintong, Shaanxi , Zaozhuang, Shandong, Huaiyuan, Anhui, Huili, Sichuan, and Mengzi, Yunnan. [0003] Pomegranate peel is the dry peel of pomegranate, which is irregular in flake or scoop shape, with different sizes and 1.5-3mm thick. The outer surface is reddish-brown, brown-yellow or dark brown, slightly shiny, rough, with many blemish-like protrusions. Some have protruding tubular persistent calyx and thick and short fruiting stems or scars. The inn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/08C07H1/08
Inventor 卢照凯谢冬养钟德品唐利萍周晓青
Owner 桂林三宝生物科技有限公司
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