Preparation method for pomegranate rind effective component having strong anti-oxidation and staphylococcus aureus inhibitory activity

A technology for staphylococcus and inhibitory activity, which is applied in the field of preparation of effective components of pomegranate peel, and can solve problems such as small yield and scale

Inactive Publication Date: 2020-02-18
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
View PDF5 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the output scale of the above-mentioned method is very small, and the level is often 100 mg o

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for pomegranate rind effective component having strong anti-oxidation and staphylococcus aureus inhibitory activity
  • Preparation method for pomegranate rind effective component having strong anti-oxidation and staphylococcus aureus inhibitory activity
  • Preparation method for pomegranate rind effective component having strong anti-oxidation and staphylococcus aureus inhibitory activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Carry out by high performance liquid chromatography (equipped with ultraviolet detector): get C18 chromatographic column, specification is length 250mm, inner diameter 10mm, with methanol as organic phase A phase, 0.1% formic acid water as water phase B phase, through liquid chromatography Adjust phase A and phase B with double pumps until the mobile phase is 15% methanol-0.1% formic acid aqueous solution, the flow rate is controlled at 2.4mL / min, the column temperature is room temperature, and the column is equilibrated until the baseline level;

[0044] Weigh 1 mg of pomegranate peel extract macroporous resin purified powder, dissolve it in 10 mL of water, filter it with a 0.45 μm organic filter, and take 20 μL of sample solution, inject it into the chromatographic column through the injector, and record the obtained chromatogram The retention time of the two chromatographic peaks of punicalagin is 21min and 40min respectively, and its chromatogram is as follows: figure...

Embodiment 2

[0051] Weigh 750mg of the purified macroporous resin of pomegranate peel extract, dissolve it in water to prepare a solution with a concentration of 500mg / mL, centrifuge to remove the suspended precipitate, place it in a refrigerator at 4°C for 15 hours, and set it aside for use, according to the example Inject 750mg of the same chromatographic column and chromatographic conditions into the chromatographic column in 1, and start to take samples after the injection, and take one component solution per minute and connect it to the container, and transfer it to the liquid phase vial for waiting. Measurement, a total of 40 samples from 0min-40min;

[0052] Using a high-performance liquid chromatograph (equipped with an ultraviolet detector and an analytical C18 chromatographic column, the specification is 250 mm in length, 4.6 mm in inner diameter, and 10 μm in particle size for filling), the recovery rate distribution of punicalagin was carried out on a series of components obtain...

Embodiment 3

[0056] Weigh 1000mg of purified macroporous resin from pomegranate peel extract, dissolve it in water to prepare a solution with a concentration of 500mg / mL, centrifuge to remove the precipitate suspended in it, and place it in a refrigerator at a temperature of 4°C for 15 hours until use; according to the examples Inject 1000 mg of sample into the chromatographic column under the same chromatographic column and chromatographic conditions in 1, start to take the sample after the injection, take one component solution per minute and connect it to the container, transfer it to the liquid phase vial for waiting Measurement, a total of 40 samples from 0min-40min;

[0057] Using a high-performance liquid chromatograph, equipped with an ultraviolet detector and an analytical C18 chromatographic column, the specifications are 250mm in length, 4.6mm in inner diameter, and 10μm in particle size, and the recovery rate distribution of punicalagin among them was carried out on a series of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Lengthaaaaaaaaaa
The inside diameter ofaaaaaaaaaa
The inside diameter ofaaaaaaaaaa
Login to view more

Abstract

The invention relates to a preparation method for a pomegranate rind effective component having strong anti-oxidation and staphylococcus aureus inhibitory activity. The method uses a macroporous resinpurified product of a pomegranate rind extract as a raw material and uses a non-linear reversed-phase chromatography technology to obtain the pomegranate rind effective component having a punicalagincontent as high as 60.6% through purification, and the pomegranate rind effective component having the strong anti-oxidation and staphylococcus aureus inhibitory activity is obtained by researching the content, recovery rate and preparation efficiency of punicalagin in the pomegranate rind effective component obtained under different sample loading amounts of an analytical column. The method hasa stable process, is very suitable for further industrial production, and lays a solid foundation for the development of novel drugs based on the pomegranate rind effective component as a bulk drug.

Description

technical field [0001] The invention relates to a preparation method of effective components of pomegranate peel with strong anti-oxidation and anti-staphylococcus aureus activities. Background technique [0002] Pomegranate, also known as pomegranate, is a fruit widely grown in my country, especially in Xinjiang (called "Western Regions" in ancient times). Skin sour, astringent, warm, with astringent intestinal diarrhea, hemostasis, deworming and other functions. Modern research shows that pomegranate peel is rich in tannin compounds, which have a wide range of biological activities. Among them, punicalagin and ellagic acid are the representatives. Especially punicalagin, its content in the medicinal material of pomegranate is about 6%, which is much higher than that of tannin, gallic acid and ellagic acid. Punicalagin has typical polyphenol antioxidant activity, in addition to a wide range of antibacterial, antiviral, antitumor and other activities. Based on the pomegr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/185A61P39/06A61P31/04
CPCA61K36/185A61K2236/51A61K2236/55A61P31/04A61P39/06
Inventor 阿吉艾克拜尔·艾萨孙光映木尼热·阿布都艾尼古丽契热·阿地力赵永昕
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap