The invention discloses an electro-conductive glass base plate cleaning agent, which is formed by components with the following percentage by weight, baking soda 3-5%, sodium tripolyphosphate 3-5%, monoethanolamine 2-4%, isopropyl alcohol 5-10%, alkyl alcohol polyoxyethylene ether 0.2-0.4%, sodium dodecylbenzene sulfonate 0.1-0.5%, ethylene glycol monobutyl ether 2-5% and the rest is water. The process for preparation comprises the following steps: adding the above raw materials into an emulsion tank in turn, and then preparing the cleaning agent after mixing, homogenizing, cooling, exhausting and filtering. The electro-conductive glass base plate cleaning agent of the invention has low pH value, when in use, the electro-conductive glass base plate cleaning agent has microvesicle or has no bubbles, which has strong water solubility, the surface of glass has no residues and particulate matters after cleaning. The cleaning agent has excellent cleanness, nontoxic, nonirritant and easy biodegradation, which is easy to discharge and process discard solution. And the cleaning agent has the advantages of simple process for preparation, high yield, only little deposition and residue, low power consumption, convenient process and operation, easy automation, no environmental contamination and high economic benefit.