8results about How to "Increase multiple" patented technology

The invention provides an in-vitro culture method of T cells, and belongs to the technical field of genetic engineering.The culture method comprises the steps that immune cell serum-free culture media are added into peripheral blood mononuclear cells, and activated culture is conducted in the presence of cell factors to obtain activated T cells; the cell factor comprises an IL-2 monoclonal antibody and a CD3 monoclonal antibody, and further comprises two or three of an iCAM1 protein, a CCL21 protein and OK432. The T cells obtained through activation and multiplication culture are relatively stable in cell phenotype, relatively large in amplification multiple, relatively high in cell viability and relatively high in tumor killing efficiency. T cells are obtained after activation is performed for 24 hours and then multiplication culture is performed for 14 days, and the number of the cells is 6.2-8.9 * 10 < 9 >; the killing rate on Hela cells is 51.9 to 74.1 percent, and the killing rate on LOVO cells is 55.7 to 80.5 percent.

The invention belongs to the technical field of bioengineering, and particularly discloses an in-vitro amplification method of canine NK cells capable of efficiently killing tumor cells and application of the canine NK cells. 3 mL of dog peripheral blood is taken, and dog peripheral blood mononuclear cells PBMC are obtained through separation; activating, amplifying and culturing canine NK cells capable of efficiently killing tumor cells; and collecting the amplified canine NK cells capable of efficiently killing the tumor cells, and storing the canine NK cells in a cryopreservation solution according to the cell density of 1 * 10 < 8 > cells / mL. According to the method, the canine NK cell with high amplification multiple can be obtained by only needing 3mL of canine peripheral blood, and the amplification multiple is high; the number of the dog NK cells of the healthy volunteer dog sample is 3.02 * 108, the amplification multiple is 1008, the total number of the dog NK cells of the tumor volunteer affected dog sample reaches 6.84 * 107, and the amplification multiple is 340; and the canine NK cell obtained by the invention has the effect of efficiently killing tumor cells.

The invention belongs to the field of immunology, and particularly relates to amplification of NK cells, in particular to a method for amplifying NK cells by using NK92 cells as trophoblast cells, which specifically uses NK92 cells secreting IL-21, IL-15 and IL-18 as trophoblast cells and PBMC as seed cells to directionally amplify and activate natural killer cells, and comprises the following steps: transfecting an NK92 cell strain through lentivirus; the NK92 cell strain capable of stably expressing IL-21, IL-15 and IL-18 is obtained; then obtaining monoclonal cells of NK92 cells through flow sorting and limit dilution methods, finally culturing and amplifying, preparing NK92 trophoblasts through irradiation, and mixing the NK92 trophoblasts with PBMC (peripheral blood mononuclear cells) for culturing so as to prepare the NK cells. According to the method, the NK cells can be efficiently amplified and activated, and the activated NK cells can improve the immunity of the organism, so that tumor and virus infection is resisted. The invention has wide application in medicine.

The invention provides a feeder cell for amplifying NK (Natural Killer) cells as well as a preparation method and application of the feeder cell. The feeder cell is a human cell with over-expression of IL-15, IL-21, 4-1BBL and IL-12p35 on a cell membrane. The human cells are selected from any one of immune cells, immortalized immune cells, lymphoma cells or leukemia cells. When the feeder cells are used for amplifying the NK cells, the amplification efficiency is high; the amplified NK cells are stronger in killing function; the feeder cell has very high clinical application value in amplification of the NK cells and large-scale clinical application of the NK cells.

This invention relates to a method for amplifying the polarization ratio of a photodetector based on a two-dimensional semi-metallic material and a heterostructure, belonging to the field of photodetection technology. Based on a strategy combining a two-dimensional semi-metallic material and a heterostructure, a BP / MoS2 / WTe2 heterojunction is constructed, where BP is the light-absorbing layer, MoS2 is the barrier layer, and WTe2 is the contact layer. This invention not only achieves selective carrier transport and reduces dark current, but also allows WTe2 to reduce contact resistance and provide a fast carrier extraction channel, realizing asymmetric amplification of the photocurrent, thereby achieving active amplification of the polarization ratio even at zero bias. This invention breaks the limitation of the inherent anisotropy of materials on the magnitude of the polarization ratio, and uses a completely dry transfer process to fabricate polarization detection photodetectors, eliminating the need for additional polarization elements and chemical solution treatment. It boasts advantages such as simple process, low cost, high repeatability, and no pollution.

The invention relates to a reagent composition for efficiently activating NK cells and application of the reagent composition. Specifically, the invention provides a culture method adopting recombinant protein PVR and / or Nectin-2 as an activating reagent, NK cells are activated and cultured in a container coated with the activating reagent, serum and trophoblast-free cells are added, and efficient amplification of the NK cells can be realized. The culture process disclosed by the invention avoids high-risk materials, is high in safety, is particularly suitable for multiplication culture of the iNK cells obtained by iPSC differentiation, and can meet the production requirements of high-quality NK cell products.

The present invention provides an in vitro culture method of T cells, which belongs to the field of genetic engineering technology. The culture method is to add immune cell serum-free culture medium to peripheral blood mononuclear cells, and perform activation culture in the presence of cytokines to obtain activated T cells; the cytokines include IL-2, CD3 monoclonal antibodies, and two or three of iCAM1 protein, CCL21 protein, and OK432. The T cells obtained by the activation and amplification culture of the present invention have a relatively stable cell phenotype, a large amplification multiple, a high cell viability, and a high tumor killing efficiency. The T cells obtained by the present invention after activation for 24 hours and then amplification culture for 14 days have a cell number of 6.2-8.9×10 9 cells; the killing rates for Hela cells were 51.9-74.1%, and for LOVO cells were 55.7-80.5%.

The invention discloses a culture medium for culturing long-life type iNKT cells and application of the culture medium, and belongs to the technical field of immune cell culture. The culture medium comprises additives of IL-2 (interleukin-2), IL-7 (interleukin-7), IL-15 (interleukin-15), alpha-GalCer (alpha-GalCer), vitamin C, glutamine and TWS119. When the culture medium is used for culturing the iNKT cells, mononuclear cells separated from peripheral blood or umbilical cord blood can be used as a source, and after initial culture and multiplication culture, the longevity type iNKT cells are collected and obtained; the anti-tumor potential of the long-life iNKT cell is further improved due to high purity and longer in-vivo survival life of the long-life iNKT cell, and the long-life iNKT cell is stronger in tumor cell killing ability, so that a foundation is laid for wide application of NKT / iNKT cell immunotherapy, and more tumor patients are benefited.