Patents
Literature
Patsnap Copilot is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Patsnap Copilot

125results about How to "Increase multiple" patented technology

High-sensitivity laminated type flexoelectric pressure sensor

A high-sensitivity laminated type flexoelectric pressure sensor comprises a metal elastic element for measuring mechanical deformation caused by micro-pressure. A plurality of flexoelectric dielectric films are attached to the top of the metal elastic element. The upper surface of each flexoelectric dielectric film and the lower surface of the flexoelectric dielectric film are provided with an upper metal electrode and a lower metal electrode respectively, each upper metal electrode and the corresponding lower metal electrode are separated through an insulating layer, the upper metal electrode of each flexoelectric dielectric film and the lower metal electrode of the flexoelectric dielectric film are respectively connected with a lead for outputting a measured load signal, each flexoelectric dielectric film together with the upper metal electrode, the lower metal electrode and the insulating layer is arranged in a shell, one end of each lead is led out of the shell, an insulating gasket is arranged between the metal elastic element and the shell, and a pressure channel for executing pressure is arranged at the lower portion of the metal elastic element. According to the high-sensitivity laminated type flexoelectric pressure sensor, pressure exerted on the films is measured through the linear relation between the exerted pressure and the stress gradient of mechanical deformation in the metal elastic element, output loads are improved through laminating, sensitivity is improved, and pressure can be accurately and easily measured.
Owner:XI AN JIAOTONG UNIV

Solar condensing mirror with adjustable focus and application thereof

The invention relates to the technical field of solar application products, in particular to a solar dish-shaped condensing mirror with adjustable focus and an application thereof. The condensing mirror is a high-reflection laminated silver-coated glass mirror, the glass mirror is mounted on a dish-shaped glass support, the support is used for carrying the glass mirror, the carrying surface is in the shape of an arc, the glass mirror and the support are connected through a focus regulating mechanism, and the glass mirror can produce elastic deformation through the regulating mechanism, thereby realizing the focus regulation of the glass mirror. The flat plate tempered glass silver mirror can be concave and deformed by tensioning the flat plate tempered glass reflecting mirror through a focus regulating support and a regulating screw rod, thereby forming the dish-shaped condensing structure; and different condensing focuses can be achieved by appropriately regulating the tensioning degree of the screw rod (the tempered glass can bear a certain elastic deformation and not be broken). A plurality of dish-shaped condensing mirror modules are mounted on a solar tracking support, respective condensing focuses of the dish-shaped condensing mirror modules are irradiated to the same position, the processing process and the mounting method of the dish-shaped condensing mirror group with the adjustable focus are very simple, the cost is low, the large-scale popularization can be realized, and the high-efficient high-power condensation can be further achieved. The high-power condensation can be applied in high-efficient gallium arsenide solar power generation, heat collection power generation, high temperature and strong light utilization and the like.
Owner:何斌

Spatial life support system urine wastewater treatment device and method

The invention relates to a spatial life support system urine wastewater treatment device and a spatial life support system urine wastewater treatment method, and belongs to the technical field of water treatment. The urine in the device is diluted by over 10 times and then delivered to a nitration reactor, meanwhile an air supply device supplies oxygen to the nitration reactor, the wastewater treated by the microbes in the nitration reactor is drained to a nitric nitrogen wastewater withdrawer, a part of the treated wastewater is recycled, the other part of the treated wastewater is deliveredto a denitrification reactor, and the wastewater further treated by the microbes in the denitrification reactor is delivered to a reverse osmosis device and treated to obtain treated water; a mixing device for mixing the microbes and the wastewater and a pH, dissolved oxygen and temperature sensor for detecting the reaction conditions in the reaction are arranged in the nitration reactor and the denitrification reactor respectively; and the reaction conditions are controlled through an exhaust valve, a gas flow meter, a pump, an automatic control valve, a heating sleeve and an acid and alkaliadder. According to the device and the method, the energy consumption is low and the concentration of the treated urine wastewater is high because only oxygen is supplied, nitrogen can be reclaimed, the treatment period is short, the safety is high, and the effect is good.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY

Preparation method for efficiently amplifying NK cells by utilizing trophoblasts

The invention relates to the field of gene engineering and cytobiology, in particular to a preparation method for efficiently amplifying NK cells by utilizing trophoblasts. The construction method comprises the following steps: (1) constructing a pHR-mbIL-21 plasmid vector, a pHR-4-1BBL plasmid vector and a pHR-MICA plasmid vector; (2) preparing recombinant lentiviruses by using pHR-mbIL-21, pHR-4-1BBL and pHR-MICA plasmid vectors respectively; (3) preparing K562-mbIL-21-4-1BBL-MICA trophoblasts; (4) extracting PBMC cells; and (5) carrying out in-vitro amplification on NK cells. The inventionprovides the preparation method of NK cells. The method employs the step of independently constructing plasmid vectors for expressing mbIL-21, 4-1BBL and MICA molecules, K562 is infected with recombinant lentivirus, the NK cells can be prepared, the preparation method overcomes the defects in the prior art, the K562 cells simultaneously expressed by IL-21, 4-1BBL and MICA molecules are used as trophoblasts for amplification culture of the NK cells, the amplification multiple of the NK cells reaches up to 890 times, the purity of the prepared NK cells reaches 92.2%, and the repeatability of theamplification multiple between different PBMC cells is good.
Owner:山东德升生物工程有限公司

Method for in vitro activating and amplifying natural killer cells

ActiveCN108004211AReduce the cost of in vitro expansion and cultureIncrease ratio and amplification factorBlood/immune system cellsCell culture active agentsMicrogramCD16
The invention belongs to the field of biological medicine, and particularly relates to a method for in vitro activating and amplifying natural killer cells. The method comprises the steps that in activator pretreatment, an activator and PBS are added into a culture flask and mixed, wherein the activator contains 0.10-100.00 mg/ml of hyaluronic acid and 0.1-20 microgram/ml of a CD16 antibody, and the volume ratio of the activator to the PBS is 1:40; activating treatment is conducted, the activator is removed, normal saline is added, the culture flask is shaken slightly, the normal saline is removed finally, and a coasted culture flask is obtained. A granulocyte-macrophage colony stimulating factor (GM-CSF) is added in a culture medium when a mononuclear cell is cultured, and the concentration is made to range from 0.05 microgram/ml to 1.00 microgram/m. Compared with an existing patented method, the method is simpler and more effective, and the in vitro amplifying and culture cost of theNK cells is effectively lowered. In the coating step, hyaluronic acid is added, and the proportion and amplification multiple of the NK cells are significantly increased; and the method has the greatmarket prospect and economic value.
Owner:深圳市赛欧细胞技术有限公司

Marine oily water disk aggregation and separation device

The invention discloses a marine oily water disk aggregation and separation device, comprising a barrel body, wherein at least a first aggregation separator and a second aggregation separator are arranged in the barrel body; the first aggregation separator and the second aggregation separator are respectively positioned on the upper side and the lower side of a fluid guide hole plate; the periphery of the fluid guide hole plate are fixedly installed on the inner wall of the barrel body in a sealing manner; the middle position of the fluid guide hole plate is provided with a fluid guide throughhole; the top end of the first aggregation separator is provided with a top part cover plate, and a gap is left between the periphery of the top part cover plate and the inner wall of the barrel body; the bottom part of the second aggregation separator is provided with a fluid guide blind plate, and a gap is left between the periphery of the fluid guide blind plate and the inner wall of the barrel body; and the first aggregation separator and/or the second aggregation separator comprise/comprises a plurality of mutually superposed ripple separating discs. The separation device has the advantages of simple structure, convenient maintenance and manufacturing, and ideal oil-water separation effect, and is particularly applicable to marine oily water separation.
Owner:DONGTAI CITY DONGFANG MARINE FITTING

In-vitro expansion method for NK (Natural Killer) cells

The invention discloses an in-vitro expansion method for NK (Natural Killer) cells. The in-vitro expansion method comprises the following steps: (1) preparing a reagent A which comprises main components of a 50 ng/ml anti-CD3 monoclonal antibody and a 50 ng/ml anti-CD16 monoclonal antibody, a reagent B which comprises a main component of 300 IU/ml IL-2, and a reagent C which comprises main components of 20 ng/ml IL-12 and 20 ng/ml IL-15, and preparing 40 ml of a lymphocyte separation liquid as a reagent D; (2) enveloping the reagent A in a T-175 culture flask, and leaving to stand overnight at 4 DEG C; (3) treating peripheral blood so as to obtain cells and serum; (4) suspending the cells, adding the reagent D, performing centrifugal collection on PBMC (Peripheral Blood Mononuclear Cell); (5) suspending the PBMC in an X-VIVO15 culture medium, adding the reagent B, the reagent C and autologous serum, and performing incubation; (6) replenishing the culture medium, the reagent B, the reagent C and the autologous serum on a third day, a sixth day, a ninth day and a twelfth day respectively, and collecting cells on a fourteenth day. The in-vitro expansion method has the beneficial effects that as the anti-CD3 monoclonal antibody and the anti-CD16 monoclonal antibody are inoculated in advance, and the cells are screened firstly, the expansion times and the purity of the NK cells in in-vitro culture are improved.
Owner:焕生汇生物基因技术(北京)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products