125results about How to "Increase multiple" patented technology

The invention discloses an organic selenium biologics and its preparation method, and method for producing selenium-rich vegetable using the same, wherein the preparation is prepared from water and the following components (by weight percentage) through mixing and fermentation, selenium dioxide : sodium hydrogen carbonate : methionine : brown sugar : carbamide : monobasic potassium phosphate : magnesium sulfate : manganese sulfate : calcium chloride = 35-45 : 35-45 : 10-20 : 45-55 : 1-10 : 1-10 : 1-4 : 1-4 : 1-10, the method of application comprises, diluting the organic selenium biologics to 50-200 times by charging water, spraying diluted organic selenium biologics liquid onto the vegetable leaves, stems or roots. The vegetable exerted by the organic selenium biologics contains rich selenium.

Automotive vehicle including a radar transceiver each including a heterodyne active IMPATT multiplier module arranged to receive a signal from a VCO, a first balance mixer arranged to receive a signal from a VCO, a second balance mixer arranged to receive a signal from a receive antenna and the IMPATT multiplier module and derive a first intermediate frequency signal, a first amplifier for amplifying the output of the second balance mixer and providing the amplifier output to the first balance mixer, and a second amplifier for amplifying the output of the first balance mixer. The vehicle also includes a processor which receives output from the second amplifier of each transceiver and generates a control signal for controlling one or more vehicular components based on the output from the second amplifier(s). The components can be part of a collision avoidance system, blind spot monitoring system and the like.

The protective film includes a transparent supporting substrate, and a protective layer that is formed on a surface of the supporting substrate and contains fluorine. The protective layer has an uneven surface structure obtained by periodically and continuously forming recesses and projections and the uneven surface structure satisfies relations of t≦390 / nb (nm) and h / t ≦0.4. Herein, t represents a period (nm) of the uneven surface structure, h represents a height (nm) which is a distance between a top of each of the projections and a bottom of each of the recesses, and nb represents a refractive index of the protective layer.

A process for generating megacaryocyte by in vitro induction to umbilical blood cell CD34+ includes in vitro amplifying the CD34+ in the culture medium containing fetal calf serum, human SCF, human TPO and / or ligand flt-3 and inducing the generation of megacaryocytes in the non-serum culture medium containing human TPO, human interleukin 3(IL-3) and / or human GM-CSF. Its advantage is high inducing efficiency.

The invention discloses clinic-level adipose-derived stem cell preparation and storage methods. Mesenchymal stem cell culture of a fat tissue source is performed by adopting a serum-free complete medium without animal source protein pollution, safety and effectiveness are realized, immunogenicity is avoided, the rate of increase of ADSCs can be better maintained, a plateau period is prolonged, an expansion multiple of each generation of cells is greatly improved, and the clinic treatment number is ensured; the serum-free complete medium is applied to preparation of clinic-level ADSCs for the first time; a fat tissue is collected from the abdomen and can be taken discontinuously repeatedly, thus avoiding damage to the adipose-derived stem cells in a liposuction process; the ADSCs are prepared by using a wall adherence method, thus increasing the primary cell yield and lowering the cost; an adopted novel serum-free cell frozen preservation solution meets a clinical application standard, reduces the use amount of DMSO, does not obviously influence the motility rate, phenotype and doubling time of the ADSCs, can be directly used in clinic infusion after reviving, is convenient to transport, and ensures the safety of clinic treatment.

A high-sensitivity laminated type flexoelectric pressure sensor comprises a metal elastic element for measuring mechanical deformation caused by micro-pressure. A plurality of flexoelectric dielectric films are attached to the top of the metal elastic element. The upper surface of each flexoelectric dielectric film and the lower surface of the flexoelectric dielectric film are provided with an upper metal electrode and a lower metal electrode respectively, each upper metal electrode and the corresponding lower metal electrode are separated through an insulating layer, the upper metal electrode of each flexoelectric dielectric film and the lower metal electrode of the flexoelectric dielectric film are respectively connected with a lead for outputting a measured load signal, each flexoelectric dielectric film together with the upper metal electrode, the lower metal electrode and the insulating layer is arranged in a shell, one end of each lead is led out of the shell, an insulating gasket is arranged between the metal elastic element and the shell, and a pressure channel for executing pressure is arranged at the lower portion of the metal elastic element. According to the high-sensitivity laminated type flexoelectric pressure sensor, pressure exerted on the films is measured through the linear relation between the exerted pressure and the stress gradient of mechanical deformation in the metal elastic element, output loads are improved through laminating, sensitivity is improved, and pressure can be accurately and easily measured.

The invention relates to the technical field of solar application products, in particular to a solar dish-shaped condensing mirror with adjustable focus and an application thereof. The condensing mirror is a high-reflection laminated silver-coated glass mirror, the glass mirror is mounted on a dish-shaped glass support, the support is used for carrying the glass mirror, the carrying surface is in the shape of an arc, the glass mirror and the support are connected through a focus regulating mechanism, and the glass mirror can produce elastic deformation through the regulating mechanism, thereby realizing the focus regulation of the glass mirror. The flat plate tempered glass silver mirror can be concave and deformed by tensioning the flat plate tempered glass reflecting mirror through a focus regulating support and a regulating screw rod, thereby forming the dish-shaped condensing structure; and different condensing focuses can be achieved by appropriately regulating the tensioning degree of the screw rod (the tempered glass can bear a certain elastic deformation and not be broken). A plurality of dish-shaped condensing mirror modules are mounted on a solar tracking support, respective condensing focuses of the dish-shaped condensing mirror modules are irradiated to the same position, the processing process and the mounting method of the dish-shaped condensing mirror group with the adjustable focus are very simple, the cost is low, the large-scale popularization can be realized, and the high-efficient high-power condensation can be further achieved. The high-power condensation can be applied in high-efficient gallium arsenide solar power generation, heat collection power generation, high temperature and strong light utilization and the like.

A ranging apparatus 1 comprises a laser light emitter 3 for emitting pulsed laser light, a reflected light receiver 4 for receiving reflected light, a distance computer 10 for finding the distance from the elapsed time until the reflected light is received, and a distance display 8 for displaying this distance. The distance computer 10 has a counter 11 for counting the frequency when the reflected light satisfies a specific condition, a table production component 12 for producing a frequency distribution table corresponding to distance by adding up the counts, a distance determiner 13 for determining as the distance to the object of measurement the point when the frequencies in the frequency distribution table exceed a threshold, and a distance selector15 for selecting a specific distance when a plurality of distances are determined, and displaying this distance on the distance display 8. As a result, when a plurality of distances are calculated, these distances can be appropriately displayed, improving the flexibility and functionality.

The invention relates to a spatial life support system urine wastewater treatment device and a spatial life support system urine wastewater treatment method, and belongs to the technical field of water treatment. The urine in the device is diluted by over 10 times and then delivered to a nitration reactor, meanwhile an air supply device supplies oxygen to the nitration reactor, the wastewater treated by the microbes in the nitration reactor is drained to a nitric nitrogen wastewater withdrawer, a part of the treated wastewater is recycled, the other part of the treated wastewater is deliveredto a denitrification reactor, and the wastewater further treated by the microbes in the denitrification reactor is delivered to a reverse osmosis device and treated to obtain treated water; a mixing device for mixing the microbes and the wastewater and a pH, dissolved oxygen and temperature sensor for detecting the reaction conditions in the reaction are arranged in the nitration reactor and the denitrification reactor respectively; and the reaction conditions are controlled through an exhaust valve, a gas flow meter, a pump, an automatic control valve, a heating sleeve and an acid and alkaliadder. According to the device and the method, the energy consumption is low and the concentration of the treated urine wastewater is high because only oxygen is supplied, nitrogen can be reclaimed, the treatment period is short, the safety is high, and the effect is good.

The invention belongs to the technology field of the cellular immunology, and particularly relates to a method for the dominant amplification of in vitro peripheral blood nature killer cells with a large amount. The method comprises the steps as follows: (1) separating single nucleus cell from human or animal peripheral blood; (2) suspending the single nucleus cell in an RPMI 1640 culture solution containing 5%-15% autologous serum and pretreating with methyl-Beta-cyclodextrin with an effective concentration of 1-4mmol / L for 36-60 hours; and (3) adding recombined interleukin 2, and amplification-culturing in an RPMI 1640 culture solution containing 5% of new-born calf serum and 5% of autologous serum for above 10 days. The method has the advantages that the blood sample amount is small; the cost is low; the operation is convenient; and the amplification multiple is high, and the nature killer cells can be amplified by 392-1752 times in a short term.

The invention discloses a lymphocyte culture fluid and culture method thereof and application therefor, the culture fluid is adding ATP and coenzyme A in completely culture fluid containing basic culture fluid RPMI-1640, albumin, L-glutamine, glucose, baking soda and HEPES. The culture method includes steps: (1) preparing culture fluid; (2) suspending cell in culture fluid prepared by step (1); (3) activating cell; (4) washing activated cell by the culture fluid prepared by step (1); (5) collecting cell. The culture fluid of the invention cultures cell with higher amplification times and longer generation time.

Automotive vehicle including a radar transceiver each including a heterodyne active IMPATT multiplier module arranged to receive a signal from a VCO, a first balance mixer arranged to receive a signal from a VCO, a second balance mixer arranged to receive a signal from a receive antenna and the IMPATT multiplier module and derive a first intermediate frequency signal, a first amplifier for amplifying the output of the second balance mixer and providing the amplifier output to the first balance mixer, and a second amplifier for amplifying the output of the first balance mixer. The vehicle also includes a processor which receives output from the second amplifier of each transceiver and generates a control signal for controlling one or more vehicular components based on the output from the second amplifier(s). The components can be part of a collision avoidance system, blind spot monitoring system and the like.

A ranging apparatus 1 comprises a laser light emitter 3 for emitting pulsed laser light, a reflected light receiver 4 for receiving reflected light, a distance computer 10 for finding the distance from the elapsed time until the reflected light is received, and a distance display 8 for displaying this distance. The distance computer 10 has a counter 11 for counting the frequency when the reflected light satisfies a specific condition, a table production component 12 for producing a frequency distribution table corresponding to distance by adding up the counts, a distance determiner 13 for determining as the distance to the object of measurement the point when the frequencies in the frequency distribution table exceed a threshold, and a distance selector 15 for selecting a specific distance when a plurality of distances are determined, and displaying this distance on the distance display 8. As a result, when a plurality of distances are calculated, these distances can be appropriately displayed, improving the flexibility and functionality.

A power supply circuit is provided which includes a first booster to boost a power supply voltage supplied from a battery and generate a first boosted voltage, a second booster to boost the power supply voltage at a higher multiplication factor than the first booster and generate a second boosted voltage, a power supply selection circuit to output the first boosted voltage or the second boosted voltage, a first smoothing capacitor placed at an output end of the power supply selection circuit, and a second smoothing capacitor placed at an output end of the second booster.

The invention relates to the field of gene engineering and cytobiology, in particular to a preparation method for efficiently amplifying NK cells by utilizing trophoblasts. The construction method comprises the following steps: (1) constructing a pHR-mbIL-21 plasmid vector, a pHR-4-1BBL plasmid vector and a pHR-MICA plasmid vector; (2) preparing recombinant lentiviruses by using pHR-mbIL-21, pHR-4-1BBL and pHR-MICA plasmid vectors respectively; (3) preparing K562-mbIL-21-4-1BBL-MICA trophoblasts; (4) extracting PBMC cells; and (5) carrying out in-vitro amplification on NK cells. The inventionprovides the preparation method of NK cells. The method employs the step of independently constructing plasmid vectors for expressing mbIL-21, 4-1BBL and MICA molecules, K562 is infected with recombinant lentivirus, the NK cells can be prepared, the preparation method overcomes the defects in the prior art, the K562 cells simultaneously expressed by IL-21, 4-1BBL and MICA molecules are used as trophoblasts for amplification culture of the NK cells, the amplification multiple of the NK cells reaches up to 890 times, the purity of the prepared NK cells reaches 92.2%, and the repeatability of theamplification multiple between different PBMC cells is good.

The invention discloses application of mixed expanded graphite serving as a lithium ion battery negative electrode material. The mixed expanded graphite material is used as the lithium ion negative electrode material to prepare a lithium ion battery; according to the negative electrode material, the weight ratio of SBR (with the solid content of 50%):CMC: Super: p is 95.5:2:1.5:1, an appropriate amount of deionized water is added to blend the materials into a slurry, the slurry is coated on copper foil, the copper foil obtained after coating is dried in a vacuum drying oven for 12 h to preparethe negative electrode plate, lM LiPF6 / EC+DEC+DMC by the ratio of 1:1:1 are adopted in electrolyte of the lithium ion battery, a diaphragm is a polypropylene microporous membrane, the electrode is the lithium plate, the battery is assembled in a closed glovebox with the argon atmosphere, charge-discharge tests are conducted on the lithium ion battery at 0.1 C magnification and 5C magnification, during charge-discharge at 0.1C magnification, the first-time charge-discharge cycle charge capacity is 2018 mAh / g, during discharge at 5C magnification, and after 500 times of cycle, the capacity retention ratio is 98.4%.

The invention belongs to the field of biological medicine, and particularly relates to a method for in vitro activating and amplifying natural killer cells. The method comprises the steps that in activator pretreatment, an activator and PBS are added into a culture flask and mixed, wherein the activator contains 0.10-100.00 mg / ml of hyaluronic acid and 0.1-20 microgram / ml of a CD16 antibody, and the volume ratio of the activator to the PBS is 1:40; activating treatment is conducted, the activator is removed, normal saline is added, the culture flask is shaken slightly, the normal saline is removed finally, and a coasted culture flask is obtained. A granulocyte-macrophage colony stimulating factor (GM-CSF) is added in a culture medium when a mononuclear cell is cultured, and the concentration is made to range from 0.05 microgram / ml to 1.00 microgram / m. Compared with an existing patented method, the method is simpler and more effective, and the in vitro amplifying and culture cost of theNK cells is effectively lowered. In the coating step, hyaluronic acid is added, and the proportion and amplification multiple of the NK cells are significantly increased; and the method has the greatmarket prospect and economic value.

The invention discloses a preparation method of CIK (cytokine-induced killer) cells. The preparation method includes the steps of 1, separating single nuclear cells from blood; 2, mixing single nuclear cells suspension with fibrin gel, disposing a mixture in a CD3McAb and RetroNectin pre-coated culture bottle, and adding a medium containing PPP and IFN-Gamma; 3, on a second day, adding IL-1Alpha and IL-2 for continual culturing, stopping culturing until the single nuclear cells are converted into CIK cells. The preparation method has the advantages that the fibrin gel is a polymer network system capable of absorbing a great amount of water and extremely similar to extracellular matrix in structure, an in-vivo cell growth microenvironment can be simulated so that sufficient growth space for cells and a spatial structure supporting inter-cell connection are provided, amplification times of the cells are significantly increased, and the obtained cells are more active.

The invention discloses a marine oily water disk aggregation and separation device, comprising a barrel body, wherein at least a first aggregation separator and a second aggregation separator are arranged in the barrel body; the first aggregation separator and the second aggregation separator are respectively positioned on the upper side and the lower side of a fluid guide hole plate; the periphery of the fluid guide hole plate are fixedly installed on the inner wall of the barrel body in a sealing manner; the middle position of the fluid guide hole plate is provided with a fluid guide throughhole; the top end of the first aggregation separator is provided with a top part cover plate, and a gap is left between the periphery of the top part cover plate and the inner wall of the barrel body; the bottom part of the second aggregation separator is provided with a fluid guide blind plate, and a gap is left between the periphery of the fluid guide blind plate and the inner wall of the barrel body; and the first aggregation separator and / or the second aggregation separator comprise / comprises a plurality of mutually superposed ripple separating discs. The separation device has the advantages of simple structure, convenient maintenance and manufacturing, and ideal oil-water separation effect, and is particularly applicable to marine oily water separation.

The invention discloses laser time-of-flight (TOF) light radar. The radar includes a transmitting module and a receiving module. The receiving module includes a first photodiode, a second photodiode and a single chip microcomputer. The single chip microcontroller includes a comparator and an analog-to-digital converter. A signal output end of the single chip microcomputer is connected with a signal input end of the transmitting module. The first photodiode and the second photodiode are arranged in parallel. Electrical signals of the first photodiode and the second photodiode are input to the comparator after high-pass filtering. An output end of the comparator is connected with an input end of the analog-to-digital converter after low-pass filtering. The analog-to-digital converter outputsa readable electrical signal. The radar has the advantages that an analog peripheral circuit of the single chip microcomputer is utilized, construction of the laser time-of-flight light radar can berealized simply with a lower efficiency requirement, thus an assembly structure is greatly simplified, and at the same time, volume and costs are reduced.

The invention relates to the technical field of biomedical science, in particular to a method for increasing the yield of immune cells. The method for increasing the yield of the immune cells comprises the steps of dilution of human peripheral blood and separation, frozen preservation, resuscitation and induction and amplification culture of the immune cells. By extracting and preserving healthy cells and conducting induction and amplification culture at a later stage, the yield of the immune cells is greatly increased; by means of a specific cell frozen preservation solution adopted in the frozen preservation process, the activity of the immune cells can be effectively maintained; laminarin is added in the induction and amplification culture process, the cells which are high in amplification times and higher in purity can be obtained, and therefore the yield of the immune cells is significantly increased.

The invention relates to the biotechnology field, in particular to CIK cell culture fluid, a CIK cell culture method and application of lentinan in CIK cell culture. The CIK cell culture fluid comprises interferon-gamma, CD3 stimulated monoclonal antibodies, interleukin-2, the lentinan and serum-free basal culture media. When the CIK cell culture fluid with the lentinan is used for CIK cell culture, PBMCs (peripheral blood mononuclear cells) can generate lymphocyte activation factors and release helper T cell factors to promote CIK cell multiplication, so that obtained CIK cells have large amplification multiple, high killing activity and high cell surface antigen content. Experiment results show that after 14-day CIK cell culture by the CIK cell culture fluid, the amplification multiple of the CIK cells is larger than 300, in-vitro killing rate (effector-target ratio being 40:1) of the CIK cells is larger than (80+ / -2)%, and the number of CIK cell surface antigens (CD3+ and CD56+) is larger than 48%.

The invention discloses a method for separating and processing marine oily water and a processing device thereof. The method comprises the following steps: preheating treatment is carried out on bilgeoily water to 48-58 DEG C; oil drop aggregation and oil-water gravity separation are carried out to separate out waste oil and separate water; the separated water is filtered into filtered water by afilter-material filter device and then is sent to a separation process device; concentrated solution is recovered; and infiltration water is discharged outside cabin. The device for processing marineoily water of the invention comprises a heater, a gravity separator, a suction pump, a filter-material filter and a membrane processor communicated sequentially; the oily water inlet of the heater isconnected to a bilge oily water station; and the infiltration water outlet of the membrane processor is communicated with a sea valve by a puff pipe. The invention not only can realize separation process of high-density residuum oily water, but also can realize effective separation process of emulsion in oily water, so as to meet strict emission standard, thereby being widely used in separation process of bilge oily water.

The invention discloses an in-vitro expansion method for NK (Natural Killer) cells. The in-vitro expansion method comprises the following steps: (1) preparing a reagent A which comprises main components of a 50 ng / ml anti-CD3 monoclonal antibody and a 50 ng / ml anti-CD16 monoclonal antibody, a reagent B which comprises a main component of 300 IU / ml IL-2, and a reagent C which comprises main components of 20 ng / ml IL-12 and 20 ng / ml IL-15, and preparing 40 ml of a lymphocyte separation liquid as a reagent D; (2) enveloping the reagent A in a T-175 culture flask, and leaving to stand overnight at 4 DEG C; (3) treating peripheral blood so as to obtain cells and serum; (4) suspending the cells, adding the reagent D, performing centrifugal collection on PBMC (Peripheral Blood Mononuclear Cell); (5) suspending the PBMC in an X-VIVO15 culture medium, adding the reagent B, the reagent C and autologous serum, and performing incubation; (6) replenishing the culture medium, the reagent B, the reagent C and the autologous serum on a third day, a sixth day, a ninth day and a twelfth day respectively, and collecting cells on a fourteenth day. The in-vitro expansion method has the beneficial effects that as the anti-CD3 monoclonal antibody and the anti-CD16 monoclonal antibody are inoculated in advance, and the cells are screened firstly, the expansion times and the purity of the NK cells in in-vitro culture are improved.

A radial inner end of a retainer is spaced radially outwardly from a radial outer end of a sliding-contact region, and with this construction the axial movement of each needle roller is limited not by the retainer but by an inner ring, and a restriction flange. Therefore, a large axial force developing in the needle rollers is received by the inner ring and the restriction flange, and as a result, the retainer having low rigidity can be satisfactorily used. Therefore, any suitable material such as a synthetic resin can be selected for the retainer, and besides the wall thickness of the retainer can be made small.

The invention discloses a method for extracting iodine from wet-process phosphoric acid produced from an iodine-containing phosphorous ore by a semi-water and bi-water method. In the process of extracting the iodine, the concentration of the iodine is 10 to 50mg / L, the phosphoric acid comprises 40 to 48 mass percent of P2O5, and the iodine is extracted by 717 anion exchange resin which has extremely high iodine ion adsorption capacity. The method comprises the following steps of enriching the iodine in the phosphoric acid; eluting; analyzing the iodine; and extracting the iodine. By the method, the used 717 anion exchange resin has high iodine enriching magnification and a high exchange speed, is easy to elute and operate, can be recycled, and reduces production cost; a selected oxidant sodium hypochlorite can easily control oxidation degrees and has an obvious effect; the method has a simple process, and a product from which the iodine is extracted has high quality and high yield; and the recovery rates of resources are improved.

The invention discloses a wavelet modulation transfer function (MTF) compensation method based on the optimal core shape, and can be applied to a ground processing system for satellite remote sensing images. The method comprises the following steps of: according to priori knowledge of noise of an imaging system, inputting a threshold value, and partially separating input digital image signals and noise in a wavelet domain; inputting parameters a and b for initial core design, performing wavelet domain modulation transfer function compensation (MTFC) processing on the signal part, adding with the noise part, performing wavelet domain normalizatioin processing and outputting process images; continuously regulating the parameters a and b, generating different cores and corresponding different process images, and measuring corresponding just noticeable difference (JND) and MTF through the process images; and recording the parameters a and b which meet the requirement, and using the parameters a and b as the optimal core parameters of the imaging system. The invention can improve the MTF for the imaging system with low ontrack dynamic MTF, improve the quality grade of the images, and improve system performance.

The invention discloses a cord blood regulative T-cell in-vitro amplification method. The method comprises the following steps: separating fresh cord blood by virtue of a ficoll density gradient centrifugal method to obtain a single karyocyte, inoculating a culture dish, which is previously coated with anti-CD3 antibody, with the single karyocyte, adding induction culture liquid, and inducing thein-vitro amplification of Tregs cells. By adopting the method provided by the invention, the Tregs cells with high purity can be obtained from the fresh cord blood by virtue of high-efficient in-vitroamplification.

A soft-error suppression circuit based on an insolation method belongs to a high reliable IC design field characterizing in using controllable transmission doors to isolate sensing nodes in the circuit so as to separate the soft-error transmission path and restrain its happening.