Method for quantifying microalgae using inorganic carbon approach

A technology of inorganic carbon and microalgae, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve the problems of inability to quantify the use of inorganic carbon by microalgae

Active Publication Date: 2012-12-19
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for identifying the pathways of inorganic carbon utilized b

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  • Method for quantifying microalgae using inorganic carbon approach
  • Method for quantifying microalgae using inorganic carbon approach

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Embodiment Construction

[0045] Embodiments of the present invention: the first step, measure the sodium bicarbonate produced by different producers, select two kinds of δ 13 Sodium bicarbonate with a C value difference greater than 8 ‰ was added to the culture medium as isotope label 1 and isotope label 2, respectively. δ of isotope-labeled sodium bicarbonate 13 C value is recorded as δ Ci , where the δ of isotope-labeled sodium bicarbonate 1 13 C value is δ C1 , the δ of isotope-labeled sodium bicarbonate 2 13 C value is δ C2 . δ of isotope-labeled sodium bicarbonate 13 C value δ C Inorganic carbon δ 13 C value δ C0 The difference is D i , where D 1 is the δ of sodium bicarbonate with isotope label 1 13 C value δ C1 Inorganic carbon δ 13 C value δ C0 the difference, D 2 is the δ of isotope-labeled sodium bicarbonate 2 13 C value δ C2 Inorganic carbon δ 13 C value δ C0 poor.

[0046] In the second step, the culture solution of two kinds of isotope-labeled sodium bicarbonate is si...

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Abstract

The invention discloses a method for quantifying a microalgae using an inorganic carbon approach. The prior art can not quantify microalgae using an inorganic carbon approach. The solving method comprises the following steps of: respectively adding two sodium bicarbonate of which the delta 13C value difference values are larger than 0.8% as an isotope label 1 and an isotope label 2 to cultivate microalgae to be tested in a nutrient solution; and respectively cultivating the microalgae to be tested for 4 days under the above condition and the cultivating condition of 16mM of sodium bicarbonate and 10mM of MAZ, testing the delta 13C values, computing the portion fB of the used and added inorganic carbon source under various microalgae cultivating conditions and the delta 13C value delta a of a microalgae body when a carbon dioxide approach in the atmosphere is completely used, and obtaining the portions of the two approaches in which the microalgae uses the inorganic carbon according to the obtained data. The method can quickly quantify the microalgae using inorganic carbon approach, and can develop a cultivating test under the complete same experiment condition to obtain the reliable data of the portion of the microalgae using inorganic carbon approach, which can not be achieved in the prior art.

Description

technical field [0001] The invention relates to a quantitative method for utilizing inorganic carbon pathways by microalgae, and belongs to the fields of ecological environment management and marine biological engineering. Background technique [0002] Microalgae (microalgae) include all tiny plants that live in water and planktonic lifestyle, usually refers to planktonic algae. The structure of microalgae is simple, and its physiological process is also relatively simple. Some species are model plants for scientific research, such as Chlamydomonas reinhardtii and Chlorella, and many species can also be cultivated artificially, which provides convenience for our research. There are two ways for microalgae to utilize inorganic carbon in water bodies. (1) Utilize carbon dioxide in the atmosphere. CO 2 As a linear non-polar molecule, it is electrically neutral, and it can freely diffuse into the cell bilayer lipid membrane and enter the CO in the cell. 2 It is used by the ph...

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Application Information

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IPC IPC(8): C12Q1/06
Inventor 吴沿友李海涛刘丛强王宝利梁小兵徐莹谢腾祥
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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