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Preparation method of punicalagin molecular imprinted polymer microspheres

A technology of punicalagin and molecular imprinting, which is applied in the field of bioengineering, can solve problems such as the preparation method of punicalagin molecularly imprinted polymer microspheres that have not yet been discovered, and achieve the effects of low cost, simple preparation process and high adsorption capacity

Inactive Publication Date: 2010-10-06
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After reviewing the prior art literature, no report related to the preparation method of the subject of the present invention, punicalagin molecularly imprinted polymer microspheres has been found so far

Method used

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  • Preparation method of punicalagin molecular imprinted polymer microspheres
  • Preparation method of punicalagin molecular imprinted polymer microspheres
  • Preparation method of punicalagin molecular imprinted polymer microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh 0.1mmol of punicalagin template molecule, 1mmol of acrylamide, and 47.2ml of acetonitrile solution in a 100ml reaction flask, ultrasonically disperse at room temperature 25°C for 1h, then add 5mmolEDMA, initiator 0.0212g AIBN, ultrasonically disperse for 1h, and blow nitrogen Deoxygenate for 20 minutes, seal, and stir at 400 rpm for 24 hours at 60°C.

[0024] The resulting mixture was centrifuged at 4000 rpm for 20 min, and the supernatant was filtered off to obtain a polymer, which was dried at 50° C. for 24 h.

[0025] The polymer was subjected to Soxhlet extraction with methanol:acetic acid (80:20, V / V) for 48 hours to remove template molecules, and was detected with a UV-Vis spectrophotometer until no template molecules were detected. The polymer obtained after the extraction was dried at 50° C. for 24 hours to finally obtain the molecularly imprinted polymer of punicalagin. The effect of its invention is as figure 1 shown.

[0026] Prepare a blank polymer ...

Embodiment 2

[0031] Weigh 0.1 mmol of punicalagin template molecule, 0.5 mmol of acrylamide, and 23.6 ml of acetonitrile solution in a 100 ml reaction flask for ultrasonic dispersion at room temperature 25°C for 1 hour, then add 5 mmol of EDMA, 0.0212 g of AIBN as an initiator, ultrasonic dispersion for 1 hour, and nitrogen gas Deoxygenate for 20 minutes, seal, and stir at 400 rpm for 24 hours at 60°C.

[0032] The resulting mixture was centrifuged at 4000 rpm for 20 min, and the supernatant was filtered off to obtain a polymer, which was dried at 50° C. for 24 h.

[0033] The polymer was subjected to Soxhlet extraction with methanol:acetic acid (80:20, V / V) for 48 hours to remove template molecules, and was detected with a UV-Vis spectrophotometer until no template molecules were detected. The polymer obtained after the extraction was dried at 50° C. for 24 hours to finally obtain the molecularly imprinted polymer of punicalagin. Its invention effect is basically equal to as figure 1 sh...

Embodiment 3

[0039] Weigh 0.05mmol of punicalagin template molecule, 1mmol of acrylamide, and 47.2ml of acetonitrile solution in a 100ml reaction flask, ultrasonically disperse at room temperature 25°C for 1h, then add 5mmol of EDMA, 0.0212g of AIBN as an initiator, ultrasonically disperse for 1h, and blow in nitrogen Deoxygenate for 20 minutes, seal, and stir at 400 rpm for 24 hours at 60°C.

[0040] The resulting mixture was centrifuged at 4000 rpm for 20 min, and the supernatant was filtered off to obtain a polymer, which was dried at 50° C. for 24 h.

[0041] The polymer was subjected to Soxhlet extraction with methanol:acetic acid (80:20, V / V) for 48 hours to remove template molecules, and was detected with a UV-Vis spectrophotometer until no template molecules were detected. The polymer obtained after the extraction was dried at 50° C. for 24 hours to finally obtain the molecularly imprinted polymer of punicalagin. The effect of its invention is basically equivalent to figure 1 sho...

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Abstract

The invention relates to the technical field of biological engineering, in particular to a preparation method of punicalagin molecular imprinted polymer microspheres. The method comprises the following steps of: dissolving a template molecule of punicalagin, a function monomer of acrylamide, a cross-linking agent of ethylene glycol dimethacrylate and an initiator of azodiisobutyronitrile into a pore-foaming agent of acetonitrile, ultrasonically dispersing the components at the normal temperature, and introducing nitrogen to seal, wherein the molar ratio of the template molecule and the function monomer is 1:5-20, the molar ratio of the function monomer and the cross-linking agent is 1:5, the volume rate of the cross-linking agent and the pore-foaming agent is 20:1000, and the mass ratio of the dosage sum of the initiator and the function monomer as well as the crossing agent is 2:100; stirring the mixed solution for 24h at 60 DEG C to carry out polymerization reaction; centrifugally separating the mixed solution and drying for 24h at 50 DEG C, and removing the template molecule by means of a Soxhlet extraction method; and drying. In the invention, the punicalagin can be selectively absorbed with high absorption quantity, and the maximum absorption quantity is up to 35299ug / g, thereby the process is simple and the cost is low.

Description

technical field [0001] The invention relates to a method in the technical field of bioengineering, in particular to a method for preparing punicalagin molecularly imprinted polymer microspheres. Background technique [0002] Punicalagin, namely 3-(S)-hexahydroxydiphenol-4,6-(S, S)-galloyl-D-glucose, also known as punicalagin. It is the skin extract of pomegranate, a plant of the pomegranate family, belonging to a kind of ellagitannin in pomegranate peel, and is the main component of ellagitannin. After punicalagin is absorbed by the human body, it can be decomposed into ellagic acid under the action of human enzymes, and it has excellent antioxidant properties. It has been used as a food antioxidant, and now it is mostly used in cosmetics in Japan and France , Compared with ellagic acid, punicalagin has better water solubility, can be used in beverages, and is easily absorbed by the human body. Like ellagic acid, punicalagin also has the effect of anti-variant and inhibitin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F222/14C08F220/56C08F2/44C08J9/26B01J20/30B01J20/26B01J20/28
Inventor 石艳张笑付志峰
Owner BEIJING UNIV OF CHEM TECH
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