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Method for purifying punicalagin on basis of isomerization characteristics

A technology of punicalagin and purification methods, which is applied in chemical instruments and methods, esterified saccharides, organic chemistry, etc., can solve problems such as unreachable, low high-purity samples, and difficult purification, and achieve strong innovation and preparation A large amount of effect

Active Publication Date: 2019-10-22
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex matrix effect of natural products, it is very difficult to purify, resulting in extremely low or even extremely high purity of qualified high-purity samples.

Method used

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  • Method for purifying punicalagin on basis of isomerization characteristics
  • Method for purifying punicalagin on basis of isomerization characteristics
  • Method for purifying punicalagin on basis of isomerization characteristics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] a. Take a pilot-scale reversed phase chromatographic column, the specification column length is 250mm×the inner diameter is 80mm, the particle size of the filler is 10μm, the mobile phase is methanol and 0.1% formic acid water with a volume ratio of 12:88, the flow rate is controlled at 180mL / min, the temperature is room temperature, and ultraviolet The detection wavelengths are 254nm and 366nm, equilibrate for 15min, ready for use;

[0022] b. Weigh 6g of pomegranate peel extract, in which the content of punicalagin is about 32%, dissolve in 20mL of water, centrifuge, centrifuge at 10,000 rpm for 5 minutes, take the supernatant, and inject it through a six-way valve. In the equilibrated pilot-scale reversed-phase column figure 1 As shown, the component α-puunigalin component 1 with a time range of 10 min to 15 min is collected in a component tank, and concentrated to 20 mL with a rotary evaporator at a temperature of 50°C;

[0023] c. Re-inject the α-puunicalagin component ...

Embodiment 2

[0025] a. Take a pilot-scale reversed phase chromatographic column, the specification column length is 250mm×the inner diameter is 80mm, the particle size of the filler is 10μm, the mobile phase is methanol and 0.1% formic acid water with a volume ratio of 12:88, the flow rate is controlled at 180mL / min, the temperature is room temperature, and ultraviolet The detection wavelengths are 254nm and 366nm, equilibrate for 15min, ready for use;

[0026] b. Weigh 6g of pomegranate peel extract, in which the content of punicalagin is about 32%, dissolve in 20mL of water, centrifuge, centrifuge at 10,000 rpm for 5 minutes, take the supernatant, and inject it through a six-way valve. In the equilibrated pilot-scale reversed-phase column figure 1 As shown, the component β-puunicalagin component 4 with a time range of 17 min to 30 min is collected in a component tank, and concentrated to 20 mL with a rotary evaporator at a temperature of 50°C;

[0027] c. Re-inject β-puunicalagin component 4 ...

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Abstract

The invention relates to a method for purifying and preparing punicalagin on the basis of isomerization characteristics. According to the method, a pomegranate peel extract is used as a raw material,and impurities contained in the pomegranate peel extract are avoided on the basis of the structural characteristic that the pomegranate peel extract has two isomers capable of being mutually converted; a large amount of punicalagin with the purity higher than 98% is obtained from the complex pomegranate peel extract by utilizing pilot-scale preparative liquid chromatography. The method is very simple and convenient, the purity of the obtained punicalagin is high, the preparation amount is large, and the method has a high reference value for purification preparation of compounds with the isomerization characteristics.

Description

Technical field [0001] The invention relates to a method for purifying punicalagin based on isomerization characteristics. Background technique [0002] Obtaining a large number of high-purity monomer compounds from complex natural product medicinal materials or extracts has always been a hot and difficult problem. Natural products have many components and very complex structure. Compounds with highly similar structures are often distributed around a single compound, which causes extremely serious interference to the target compound during the separation process. This interference is often called matrix effect. In traditional column chromatography, liquid chromatography, and countercurrent chromatography, this matrix effect often brings great difficulties to the separation of pure compounds, and even products with qualified purity cannot be obtained. Therefore, inventing a separation method that can effectively shield the matrix effect and can be applied to simple chromatographi...

Claims

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Application Information

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IPC IPC(8): C07H13/08C07H1/08
CPCC07H13/08C07H1/08C07H9/02B01D15/325B01D15/24B01D15/1814C07H19/01
Inventor 阿吉艾克拜尔·艾萨孙光映赵永昕木尼热·阿布都艾尼古丽契热·阿地力
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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