Purification method of pomegranate peel punicalagin

A technology of punicalagin and a purification method, which is applied in the field of purification of punicalagin from pomegranate peel, can solve the problems of being unsuitable for industrial production, low yield of purified products, complicated purification process, etc., and achieves low cost, large amount of preparation, and simple purification steps. simple effect

Active Publication Date: 2018-11-23
XINJIANG MEDICAL UNIV
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Problems solved by technology

[0004] However, in the prior art, when macroporous adsorption resins are used to separate and purify punicalagin in pomegranate peel, the technology of combining repeated column chromatography and high-efficiency capillary electrophoresis is often used. The purification process is complex, costly, and time-consuming. Low, not suitable for industrial production

Method used

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  • Purification method of pomegranate peel punicalagin
  • Purification method of pomegranate peel punicalagin
  • Purification method of pomegranate peel punicalagin

Examples

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Embodiment 1

[0042] Grind Xinjiang pomegranate peel and pass through 80-mesh sieve, add the obtained pomegranate peel powder into ethanol with a volume fraction of 60% according to the ratio of solid to liquid 1:24, extract at room temperature for 30 minutes, filter through gauze, and filter the obtained filtrate Concentrate under reduced pressure at 50°C until there is no alcohol smell, dilute the resulting concentrated solution with water to a solution with a mass concentration of 20.8 mg / mL, adjust its pH to 2.0 with hydrochloric acid with a mass concentration of 1.2 mol / L, and obtain pomegranate Pian punicalagin crude extract;

[0043] Swell the macroporous adsorption resin in 95% ethanol solution by volume fraction for 24h, wet-pack the column, and elute with 95% ethanol to a mixture of eluent and distilled water (volume ratio 1:5) No white turbidity; and use ultraviolet spectrophotometry, with 95% ethanol as a blank control, until the eluate has no absorption in the wavelength range of...

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Abstract

The invention provides a purification method of pomegranate peel punicalagin. According to the purification method, macroporous adsorption resin is used for purifying and the content of punicalagin inthe obtained pomegranate peel punicalagin can reach 85 to 95 percent; a technology has good stability and a testing foundation is laid for further researches of an anti-tumor effect of the punicalagin; the column volume of the macroporous adsorption resin is magnified for 23 times; a purification technology is determined through a magnification test and the obtained punicalagin content and extract rate are basically consistent to results of small experiments of laboratories, so that the purification technology is stable and feasible; pilot experiments can be further carried out so that a testing foundation is laid for subsequent further development and utilization of the punicalagin.

Description

technical field [0001] The invention relates to the technical field of separation and purification of polyphenolic substances, in particular to a method for purifying punicalagin from pomegranate peel. Background technique [0002] In terms of pharmacological research on pomegranate, the mixture of pomegranate extract and pomegranate juice is mostly used as the research object, and there are few studies on single active ingredients with high purity. With the in-depth research on the physiological activity of punicalagin in recent years, the market demand for punicalagin has gradually increased. However, the preparation, separation and purification process of punicalagin is still obtained by repeated column chromatography, which requires a large amount of Solvents are time-consuming and labor-intensive. [0003] Macroporous adsorption resin is a kind of organic polymer adsorbent with good adsorption performance developed in the late 1970s. It is an organic polymer insoluble ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/08C07H1/08
CPCC07H1/08C07H13/08
Inventor 马桂芝高晓黎邱慧敏
Owner XINJIANG MEDICAL UNIV
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