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Method for separating and purifying punicalagin in pomegranate peel

A punicalagin, separation and purification technology, applied in chemical instruments and methods, organic chemistry, esterification saccharides and other directions, can solve the problems of high energy consumption, complex process, low extraction rate and the like, and achieves low operating cost and extraction rate. High, step-by-step effects

Active Publication Date: 2017-01-25
陕西工业职业技术学院
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Problems solved by technology

The existing extraction method of punicalagin has complex process, high energy consumption and low extraction rate

Method used

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  • Method for separating and purifying punicalagin in pomegranate peel
  • Method for separating and purifying punicalagin in pomegranate peel
  • Method for separating and purifying punicalagin in pomegranate peel

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Embodiment Construction

[0054] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present invention, and should not be regarded as limiting the scope of the present invention.

[0055] 1. Test plan

[0056] 1. Preparation of punicalagin crude extract

[0057]Put the pomegranate peel in an oven at 50°C to dry, crush, pass through a 100-mesh sieve to obtain pomegranate peel powder; then mix the pomegranate peel powder with 60% ethanol at a ratio of 1g::25mL of solid-liquid ratio, and heat at 60°C extraction under the conditions for 6 hours, suction filtration twice, and the combined filtrates; finally, the combined filtrates were concentrated by rotary evaporation at 50° C. to obtain crude extracts of punicalagin with different concentrations.

[0058] 2. Resin type screening

[0059] Take the macroporous resin products LX-620, LX-280, LX-1010, and L...

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Abstract

The invention discloses a method for separating and purifying punicalagin in pomegranate peel. The method includes the steps of 1, carrying out crude extraction, namely grinding the pomegranate peel to obtain pomegranate peel powder, and extracting the pomegranate peel powder by extraction ethyl alcohol so as to obtain crude extraction liquid of the punicalagin; 2, adsorbing, namely taking the crude extraction liquid of the punicalagin as adsorption liquid, and subjecting the crude extraction liquid of the punicalagin to resin adsorption by macroporous resin so as to obtain adsorption resin; 3, desorbing, namely subjecting the adsorbent resin to desorption by the ethyl alcohol and collecting eluent; 4, purifying, namely concentrating the eluent and purifying the concentrated eluent by means of high-speed counter-current chromatography so as to obtain a purified punicalagin product. The method for separating and purifying the punicalagin in the pomegranate peel has the advantages of simple steps, low industrialized operation cost, high punicalagin extraction ratio, excellent purification effect and capability of enabling the purity of the punicalagin in the purified punicalagin product to be above 90%.

Description

technical field [0001] The invention relates to the technical field of separation and purification methods for polyphenols, in particular to a separation and purification method for punicalagin in pomegranate peel. Background technique [0002] Punicalagin (Punicalagin) belongs to polyphenols, the molecular formula is C 48 h 28 o 30 , is the main active ingredient in the skin extract of pomegranate (Punica granatum L.), which has various pharmacological effects such as anti-oxidation, anti-cancer, anti-bacterial, anti-viral, and anti-inflammatory. Punicalagin has been proven to have excellent antioxidant properties and can be widely used as an antioxidant in the food industry, and is now used in cosmetics in Japan and France. At present, punicalagin is prepared by natural extraction methods, and the main raw material for extraction is pomegranate peel. The existing extraction method of punicalagin has complex process, high energy consumption and low extraction rate. Co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/08C07H1/08
CPCC07H1/08C07H13/08
Inventor 刘迪李婧宋晓宇
Owner 陕西工业职业技术学院
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