Composition with skin anti-sugar effect and application of composition in cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a composition with a skin anti-sugar effect and application of the composition in cosmetics. The composition comprises the following components in parts by weight: 5-20 parts of a grapefruit extract, 10-25 parts of rhamnose, 10-25 parts of a sophora flower bud extract and 45-80 parts of a cyclodextrin derivative. According to the invention, the sophora flower bud extract, the grapefruit extract, the rhamnose and the cyclodextrin derivative are specifically combined to obtain the raw material composition with a remarkable anti-sugar effect; the sophora flower bud extract, the grapefruit extract and the rhamnose in the composition act on different targets to synergistically enhance the AGEs inhibition effect, so that the composition has a remarkable anti-sugar effect, and the good effects of improving skin elasticity, removing wrinkles and the like are achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a composition with skin anti-sugar effect and application thereof in cosmetics. Background Art

[0002] The causes of wrinkles and loss of skin elasticity can be divided into internal and external factors. The internal factors are mainly the natural aging of the organism, bad eating habits and living habits, such as staying up late and lack of exercise; the external factors are mainly ultraviolet rays and environmental pollution. The combined action of endogenous and exogenous factors will accelerate the glycation reaction in the body. Glycation reaction, also known as the Maillard reaction, refers to the non-enzymatic condensation reaction between the carbonyl group on the reducing sugar (such as glucose) and the free amino group on macromolecules such as proteins, lipids or nucleic acids. The covalent adducts generated by the glycation reaction are collectively referred to as advanced glycation end products (AGEs). Excessive advanced glycation end products in the body will undergo glycation cross-linking reactions with the elastin and collagen of the skin, causing the skin to lose elasticity and produce wrinkles.

[0003] As the anti-sugar cosmetics market continues to grow, consumers are increasingly demanding safe and effective anti-sugar products. However, a single ingredient is often difficult to achieve the desired anti-sugar effect, so it is particularly important to develop a composite anti-sugar technology that can synergistically enhance the effect. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a composition with skin anti-sugar effect and its application in cosmetics, so as to achieve effective inhibition of AGEs and synergistically enhance the anti-sugar effect of the product.

[0005] To achieve the above objectives, the technical solutions adopted by the present invention include:

[0006] In a first aspect, the present invention provides a composition with skin anti-sugar efficacy, comprising the following components in parts by weight: 5-20 parts of grapefruit extract, 10-20 parts of rhamnose, 10-25 parts of Sophora japonica bud extract and 35-75 parts of cyclodextrin derivatives.

[0007] After research, it was found that the extract of Sophora japonica bud contains isoquercetin, which has obvious anti-sugar effect. Its anti-sugar mechanism is to inhibit the production of AGEs by affecting the proteins involved in the glycosylation reaction. Specifically, the extract of Sophora japonica bud containing isoquercetin mainly forms a stable complex with tryptophan, glycine, leucine, tyrosine, aspartic acid and arginine on the protein through hydrogen bonding and π-π stacking, which masks the amino group in the protein, thereby preventing the carbonyl group in the reducing sugar from undergoing glycosylation reaction with it, thereby inhibiting the formation of AGEs; grapefruit extract contains naringin, which can also play an anti-sugar effect by inhibiting the production of AGEs, and the grapefruit extract containing naringin mainly plays an anti-sugar effect by interacting with arginine, lysine, cysteine, glutamic acid and valine on the protein; rhamnose can reduce the production of AGEs by competing with the reducing sugars involved in the glycosylation reaction to bind to the protein, and at the same time inhibit the damage of AGEs to fibroblasts, thereby playing an auxiliary anti-sugar effect, but it cannot show significant anti-sugar effect when used alone.

[0008] Therefore, the present invention selects the above-mentioned specific components and acts on different targets to synergistically enhance the inhibitory effect on AGEs, so that the composition has a significant anti-sugar effect. The sophora japonica bud extract containing isoquercetin and the grapefruit extract containing naringin in the composition of the present invention are replaced with other plant materials with similar functions but not containing isoquercetin or naringin, and the anti-sugar effect of the obtained composition is significantly reduced. At the same time, the combination of rhamnose with the sophora japonica bud extract and the grapefruit extract in the composition of the present invention has a synergistic effect.

[0009] In addition, the inventors explored the dosage of each component and found that when the dosage of the grapefruit extract, Sophora japonica bud extract and rhamnose is too small or the total dosage of the three components is too small, the synergistic effect is not obvious, and when the dosage of the three components is too large or the total dosage of the three components is too large, antagonism may occur, which will also lead to a decrease in the effect of inhibiting AGEs. This shows that the above three components have a more ideal synergistic effect when they are within the specified range of the present invention.

[0010] Preferably, the composition comprises the following components in parts by weight: 10-15 parts of grapefruit extract, 10-15 parts of rhamnose, 15-20 parts of sophora japonica bud extract and 50-65 parts of cyclodextrin derivative.

[0011] Preferably, the composition comprises the following components in parts by weight: 10 parts of grapefruit extract, 10 parts of rhamnose, 20 parts of sophora japonica bud extract and 60 parts of cyclodextrin derivative.

[0012] It has been found through experiments that when the components of the present invention are combined in the optimal dosage ratio defined above, the obtained composition has the best inhibitory effect on AGEs, thereby exerting an excellent anti-sugar effect.

[0013] Preferably, the cyclodextrin derivative includes at least one of hydroxypropyl-α-cyclodextrin, hydroxypropyl-β-cyclodextrin, hydroxypropyl-γ-cyclodextrin, and sulfobutyl-β-cyclodextrin.

[0014] Preferably, the cyclodextrin derivative is hydroxypropyl-β-cyclodextrin or hydroxypropyl-γ-cyclodextrin.

[0015] More preferably, the cyclodextrin derivative is hydroxypropyl-β-cyclodextrin.

[0016] Experimental investigations have shown that the addition of cyclodextrin derivatives can effectively improve the stability of the product and the bioavailability of the active ingredients. When hydroxypropyl-β-cyclodextrin is used as a cyclodextrin derivative to prepare the anti-glycemic composition of the present invention, hydroxypropyl-β-cyclodextrin can effectively improve the encapsulation and release properties of the active ingredients and enhance the skin's absorption of anti-glycated ingredients. At the same time, it can also reduce the skin's damage to glycated products, thereby providing more comprehensive anti-glycated protection.

[0017] In a second aspect, the present invention provides the use of the composition with skin anti-sugar efficacy in the preparation of anti-sugar products.

[0018] Preferably, the product is a cosmetic; the cosmetics include leave-on cosmetics and rinse-off cosmetics.

[0019] In a third aspect, the present invention provides a cosmetic having anti-sugar effect on skin, wherein the cosmetic comprises the composition having anti-sugar effect on skin.

[0020] Preferably, the mass percentage of the composition with skin anti-sugar efficacy in the cosmetics is 0.5-5%.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The present invention obtains a raw material composition with significant anti-sugar effect by specifically combining Sophora japonica bud extract, grapefruit extract, rhamnose and cyclodextrin derivatives; the Sophora japonica bud extract, grapefruit extract and rhamnose in the composition act on different targets to synergistically enhance the inhibitory effect on AGEs, so that the composition has significant anti-sugar effect. At the same time, the selection of the composition of the present invention is specific, and if the types in the composition are replaced, the anti-sugar effect will be significantly reduced. DETAILED DESCRIPTION

[0023] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. It should be understood by those skilled in the art that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0024] Unless otherwise specified, all raw materials involved in the following examples and comparative examples are commercially available general materials.

[0025] The preparation method of the composition described in the examples and comparative examples is to uniformly mix the components of the composition in a conventional manner to obtain the composition.

[0026] Examples 1-8

[0027] Table 1: Formula of the composition with skin anti-sugar effect described in Examples 1-8 (parts by weight)

[0028]

[0029] Comparative Examples 1-8

[0030] Table 2: Formula of the composition with skin anti-sugar effect described in Comparative Examples 1-8 (parts by weight)

[0031]

[0032] Comparative Example 9

[0033] This comparative example provides a composition with skin anti-sugar efficacy, which differs from Example 1 in that tea tree flower extract replaces Sophora japonica bud extract, and the other components and contents remain unchanged.

[0034] Comparative Example 10

[0035] This comparative example provides a composition with skin anti-sugar effect, which is different from Example 1 in that pomegranate extract replaces grapefruit extract, and the other components and contents remain unchanged.

[0036] Effect Example 1

[0037] 1. Anti-sugar effect test

[0038] The compositions prepared in Examples 1-8 and Comparative Examples 1-10 were used as samples and dissolved in 0.05 mol / L PBS buffer (pH=7.4) to prepare 1% sample solutions. Then, 200 μL of different sample solutions were accurately pipetted and mixed with 300 μL BSA-glucose saccharification model solution (BSA-glucose saccharification model solution: 0.05 mol / L PBS buffer (pH=7.4) was used to prepare a mixed solution containing 8 mg / mL bovine serum albumin (BSA) and 0.2 mol / L glucose, and filtered through a 0.22 μm filter membrane) and incubated at 55° C. for 7 days. After the reaction was completed, a fluorescence microplate reader was used to detect the change in AGEs content under the conditions of an excitation wavelength of 370 nm and an emission wavelength of 440 nm, and the AGEs inhibition rate was calculated. The AGEs inhibition rate of each composition is shown in Table 3 below.

[0039] AGEs inhibition rate (%) = [1-(AB) / (CD)]*100%

[0040] Where:

[0041] A: Fluorescence intensity of the saccharification system of the sample group;

[0042] B: Fluorescence intensity of PBS solution in sample group;

[0043] C: fluorescence intensity of the saccharification system without adding sample group;

[0044] D: Fluorescence intensity of PBS solution without sample addition.

[0045] Table 3

[0046]

[0047]

[0048] The results in Table 3 show that the AGEs inhibition rates of the compositions prepared in Examples 1-8 all reached more than 90%, and had significant anti-glycemic effects. Among them, the AGEs inhibition rate of the composition prepared by the technical solution of Example 1 reached 99.61%, and had the best anti-glycemic effect.

[0049] When any one or two of the three components of grapefruit extract, rhamnose and sophora japonica bud extract are missing in Comparative Examples 1-6, the inhibitory effect of the obtained components on AGEs is significantly reduced. In addition, when the sophora japonica bud extract and grapefruit extract are replaced by other extracts with anti-glycemic effects in Comparative Examples 9-10, the AGEs inhibition rate of the obtained composition is also significantly reduced compared with the embodiment. The above results all show that there is an obvious synergistic effect between the specific components selected by the present invention, especially the sophora japonica bud extract, grapefruit extract and rhamnose. The three cooperate with each other and influence each other, which can significantly improve the anti-glycation effect of the product, and each component is indispensable; when the dosage ratio of the sophora japonica bud extract, grapefruit extract, rhamnose and cyclodextrin derivative in Comparative Examples 7-8 exceeds the specified range of the present invention, the inhibitory effect of the obtained composition on AGEs is significantly reduced, indicating that the components of the present invention can better play a synergistic effect within a specific dosage range, thereby maximizing the anti-glycemic effect of the product.

[0050] Effect Example 2

[0051] This effect example provides an emulsion with skin anti-sugar effect, which includes the composition with skin anti-sugar effect prepared in Example 1. The specific formula and process are as follows:

[0052] The emulsion comprises the following components in percentage by mass: 1% of the composition with skin anti-sugar effect (Example 1), 0.03% of disodium EDTA, 4% of glycerol, 2% of propylene glycol, 0.03% of hyaluronic acid, 0.5% of 1,2-hexanediol and the balance of water.

[0053] The emulsion with skin anti-sugar effect is obtained by stirring and mixing the above components evenly, and is used for subsequent human trials.

[0054] 1. Security testing

[0055] The above-prepared emulsion with skin anti-sugar effect was used as a sample to test its safety. The specific method is as follows:

[0056] Number of testers: 35 people, including 12 males and 23 females, aged 19-48 years old. The volunteers met the selection criteria for subjects in the human skin patch test in the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0057] Test method: The lotion is subjected to a patch test. Qualified patch test equipment is selected. With the closed patch test method, about 0.020-0.025 ml of the test substance is placed in the patch tester. The test substance is applied to the curved side of the forearm of the subject with external medical tape. The test substance is evenly applied to the skin by lightly pressing with the palm of the hand. After 24 hours, the test substance patch tester is removed. The skin reaction is observed at 0.5, 24, and 48 hours after the removal of the test substance patch tester. The results are recorded according to the skin reaction grading standard in the "Safety Technical Specifications for Cosmetics" (2015 edition). Table 4 shows the grading standard for adverse skin reactions.

[0058] Table 4

[0059]

[0060] The results of the human skin closed patch test showed that there was no positive reaction in 35 people. According to the "Technical Specifications for Safety of Cosmetics" (2015 edition), the test substance will not cause adverse skin reactions in this batch of subjects.

[0061] 2. Human testing

[0062] The 35 subjects selected above were subjected to human trials to explore the trial effects of the prepared emulsion containing the anti-sugar effect composition. The cheeks of the subjects were selected as the test site, and the emulsion was used once in the morning and evening after cleansing, with 1-2 mL of sample each time. After continuous use for one month, the subjects evaluated the effects of the emulsion on improving wrinkles, skin texture, skin elasticity, etc. The results are shown in Table 5.

[0063] Table 5

[0064]

[0065]

[0066] From the results in Table 5, it can be seen that the basic emulsion prepared by the composition with skin anti-sugar efficacy of the present invention has a good anti-sugar effect on the subjects after one month of use, thereby playing a good role in removing wrinkles and improving skin elasticity, with an effective rate of more than 85%, and the effect is remarkable.

[0067] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A composition having skin anti-sugar effect, characterized in that: The composition comprises the following components in parts by weight: 5-20 parts of grapefruit extract, 10-20 parts of rhamnose, 10-25 parts of sophora japonica bud extract and 35-75 parts of cyclodextrin derivative.

2. The composition with skin anti-sugar effect according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 10-15 parts of grapefruit extract, 10-15 parts of rhamnose, 15-20 parts of sophora japonica bud extract and 50-65 parts of cyclodextrin derivative.

3. The composition with skin anti-sugar effect as claimed in claim 2, characterized in that: The composition comprises the following components in parts by weight: 10 parts of grapefruit extract, 10 parts of rhamnose, 20 parts of sophora japonica bud extract and 60 parts of cyclodextrin derivative.

4. The composition with skin anti-sugar efficacy according to any one of claims 1 to 3, characterized in that: The cyclodextrin derivative includes at least one of hydroxypropyl-α-cyclodextrin, hydroxypropyl-β-cyclodextrin, hydroxypropyl-γ-cyclodextrin and sulfobutyl-β-cyclodextrin.

5. The composition with skin anti-sugar effect as claimed in claim 4, characterized in that: The cyclodextrin derivative is hydroxypropyl-β-cyclodextrin or hydroxypropyl-γ-cyclodextrin.

6. The composition with skin anti-sugar effect as claimed in claim 5, characterized in that: The cyclodextrin derivative is hydroxypropyl-β-cyclodextrin.

7. Use of the composition with skin anti-sugar efficacy as claimed in any one of claims 1 to 6 in the preparation of anti-sugar products.

8. The use according to claim 7, characterized in that The product is a cosmetic; the cosmetics include leave-on cosmetics and rinse-off cosmetics.

9. A cosmetic having an anti-sugar effect on the skin, characterized in that: The cosmetic comprises the composition with skin anti-sugar efficacy according to any one of claims 1 to 6.

10. The cosmetic with skin anti-sugar effect according to claim 9, characterized in that: The mass percentage of the composition with skin anti-sugar effect in cosmetics is 0.5-5%.

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