Probiotic composition and application thereof

A composition and technology of probiotics, applied in the field of microorganisms, can solve problems such as the lack of research on probiotic compound formulas, achieve the effects of lowering fasting blood sugar levels, reducing epididymal fat content, and wide application prospects

Active Publication Date: 2019-04-23
INFINITUS (CHINA) CO LTD
View PDF11 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above patents all use a single probiotic or a combination of probiotics and prebiotics or Chinese herbal medicines to relieve metabolic syndrome, and currently there are few probiotic compound formulas that can synergistically improve metabolic syndrome. Research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Probiotic composition and application thereof
  • Probiotic composition and application thereof
  • Probiotic composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0040] The present invention also provides a preparation method of the probiotic composition, inoculating the liquids of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 into the improved MRS medium respectively, at 35-37°C Cultivate under anaerobic conditions for 18-24 hours, collect the bacteria; resuspend with freeze-drying protectant respectively to make the content of each bacteria reach 10 10 CFU / mL or more, then the suspension was cultured under anaerobic conditions at 37°C for 40-60 minutes, dried to obtain freeze-dried bacterial powder of each bacterial cell, and the number of viable bacteria in the bacterial powder was confirmed by resuspension and dilution plate coating; The freeze-dried bacteria powder of Bifidobacterium adolescentis CCFM8630, the freeze-dried bacteria powder of Lactobacillus reuteri CCFM8631 and the freeze-dried bacteria powder of Lactobacillus rhamnosus CCFM1044 are compounded and mixed in ...

Embodiment 1

[0073] Example 1: Probiotic composition has good tolerance to simulated gastrointestinal fluid

[0074] Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 stored at -80°C were inoculated in modified MRS medium (mMRS) respectively, and cultured in an anaerobic workstation at 37°C for 36 hours. Subculture 2 times with the inoculation amount of 2%-4% (v / v), adjust the live bacteria concentration of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 culture solution to be 5×10 8 CFU / mL, take the concentration as 5×10 8 CFU / mL of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 culture solution 1mL each, mix well, take 1mL of mixed culture solution and 9.0mL pH 2.5 artificial simulated gastric juice (containing 1% stomach Protease, mMRS medium with pH=2.5) mixed, cultured in anaerobic environment at 37°C, samples ...

Embodiment 2

[0081] Example 2: The probiotic composition has no toxic and side effects on C57BL / 6J mice

[0082] Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 with a live bacterial count ratio of 1:1:1 were resuspended in 3% sucrose solution to make a concentration of 1.0×10 9 CFU / mL bacterial suspension. Eight healthy male C57BL / 6J mice with a body weight of about 20g-22g were taken, and after one week of adaptive feeding, they were fed with the bacterial suspension of this concentration once a day, observed for one week, and the death and body weight were recorded. The experimental results are shown in Table 3.

[0083] Table 3 Changes in body weight and death of mice

[0084] time (days)

1

2

3

4

5

6

7

weight(g)

20.2±0.9

20.4±1.0

20.5±1.3

20.7±1.1

20.8±0.8

21.2±0.9

21.6±0.8

Circumstances of death

-

-

-

-

-

-

-

[0085] Note: -: no dea...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of microbes, and discloses a probiotic composition and application thereof. The probiotic composition consists of bifidobacterium adolescentis CCFM8630, lactobacillus reuterii CCFM8631 and lactobacillus rhamnosus CCFM1044, the effect of relieving metabolic syndromes is significantly better than that of CCFM8630 or CCFM8631 used alone and in combination,especially in fields of reducing an epididymal fat content, reducing a fasting blood glucose level, reducing a glucose tolerance curve lower area, lowering a serum low-density lipoprotein and total cholesterol content, increasing the liver antioxidant capacity and reducing a serum IL-1beta content, the decrease or increase of the composition is increased by 9.62%-769.62% compared with a single bacteria or double bacteria combination formula, and the triple bacteria combination can achieve significant synergy.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a probiotic composition and its application, in particular to a probiotic composition for alleviating metabolic syndrome and its application. Background technique [0002] Metabolic syndrome is a group of diseases closely related to obesity, diabetes, hypertension, and cardiovascular disease, and its clinical manifestations are abnormal glucose tolerance, overweight or obesity, dyslipidemia, and hypertension. Numerous studies have shown that metabolic syndrome is a major contributor to cardiovascular disease, and people with metabolic syndrome are also more likely to develop inflammation and are also prone to other diseases, such as polycystic ovary syndrome, fatty liver, cholesterol gallstones, Asthma, sleep disturbances, and certain cancers. In addition, metabolic syndrome is significantly associated with the prevalence and mortality of coronary atherosclerotic heart di...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23L29/00A23L33/00A23L11/00A23L19/00A23C9/123A23C13/16A23C19/032A23C19/06A61K35/747A61P3/04A61P3/10A61P3/06A61P29/00A61P39/06A61K35/745C12R1/01C12R1/225A23L11/50A23L11/60
CPCA61P3/04A61P3/06A61P3/10A61P29/00A61P39/06A23C9/1234A23C13/16A23C19/0323A23C19/062A23L19/00A23L29/065A23L33/00A23L33/135A61K35/745A61K35/747C12N1/20A23V2002/00A23L11/50A23V2400/173A23V2400/513A23V2400/175A23V2200/332A23V2200/328A23V2200/3262A23V2200/30A61K2300/00A61P1/00A23L33/175A61K2035/115
Inventor 王园园陈卫王刚朱晓风马方励肖俊勇张灏赵建新
Owner INFINITUS (CHINA) CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap