Method for detecting effective hypolipidemic component in edible fungus by establishing foam cell model

A foam cell and model detection technology, applied in biochemical equipment and methods, material stimulation analysis, microbial measurement/inspection, etc., to achieve the effects of small environmental factors, promoted lipid outflow, and low drug consumption

Inactive Publication Date: 2012-07-11
NANJING NORMAL UNIVERSITY
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Problems solved by technology

[0004] In order to solve the deficiencies in the prior art, the present invention provides a method for constructing a foam cell model

Method used

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  • Method for detecting effective hypolipidemic component in edible fungus by establishing foam cell model
  • Method for detecting effective hypolipidemic component in edible fungus by establishing foam cell model
  • Method for detecting effective hypolipidemic component in edible fungus by establishing foam cell model

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Embodiment Construction

[0018] 1. Build a foam cell model. The macrophage RAW264.7 (the cell line is the product of ATCC in the United States, sold by Shanghai Shenke Biotechnology Co., Ltd.) was seeded in a 24-well plate, and the inoculation volume in each well was 2×10 4 A cell in the logarithmic phase, the cell culture condition is 10% DMEM medium, the temperature is 37 degrees Celsius, and the carbon dioxide concentration is 5%. Cultivate until the cell adhesion rate reaches 60-80% to carry out foam cell construction and dosing experiments, and set up normal group, model group, positive control group and dosing group respectively. In the normal group, the normal macrophage RAW264.7 with an adhesion rate of 60-80% was cultured for 24 hours; in the model group, the final concentration of 70 μg / mL of oxidized low-density lipoprotein (OX-LDL) was induced, and foam cells were formed after continuing to culture for 24 hours; the positive control group was macrophages with a final concentration of 70u...

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Abstract

The invention belongs to a method for screening an effective hypolipidemic component in medicated edible fungus by establishing a foam cell model in cytobiology. The method comprises the following steps of: inducing a macrophage RAW264.7 by using an oxidized low-density lipoprotein (OX-LDL) to cause fatty degeneration to obtain a foam cell; combining oil red O staining and measuring the content of total cholesterol and the contents of free cholesterol and cholesterol ester in the cell to detect the effusion condition of cholesterol in the cell to achieve the effects of checking whether the macrophage has fatty degeneration and damage or not and treating the macrophage having fatty degeneration by use of medicines; and detecting and screening the effective hypolipidemic component in the edible fungus through detecting the treatment effect on the foam cell model.

Description

technical field [0001] The invention belongs to the method of constructing a foam cell model in biological cytology to screen the effective ingredients for reducing blood fat in medicinal edible fungi. Background technique [0002] Foam cells refer to lipid-rich phagocytes. The lipids in the cytoplasm were dissolved and extracted by organic solvents during the production process, and the stained foamy structure was named after it. Foam cell formation is an early event in the formation of atherosclerosis. When the vascular endothelium is damaged, monocytes in the blood pass through the endothelial gap and transform into macrophages under the intima. Macrophages mediate the oxidative modification of low-density lipoprotein cholesterol infiltrated into the subendothelium of blood vessels to form oxidized low-density lipoprotein cholesterol, and engulf a large amount of oxidized low-density lipoprotein cholesterol mainly through type A scavenger receptors, resulting in intracel...

Claims

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Application Information

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IPC IPC(8): C12Q1/44C12Q1/02G01N21/64
Inventor 陆玲雍阳阳陈晶晶
Owner NANJING NORMAL UNIVERSITY
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