Total cholesterol detection reagent with high accuracy and disturbance resisting capacity

A technology for total cholesterol and detection reagents, applied in the field of total cholesterol detection reagents, to achieve the effect of improving stability, improving accuracy and removing interference

Active Publication Date: 2016-03-09
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has good precision and accuracy, and high sensitivity, but it is easily interfered by various interference factors. For example, substances in serum such as hemoglobin, bilirubin, ascorbic acid, etc. will also interfere with the determination results. Based on the invention of a total cholesterol detection reagent with good accuracy and stronger anti-interference ability

Method used

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  • Total cholesterol detection reagent with high accuracy and disturbance resisting capacity
  • Total cholesterol detection reagent with high accuracy and disturbance resisting capacity
  • Total cholesterol detection reagent with high accuracy and disturbance resisting capacity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1) A total cholesterol detection reagent with high accuracy and strong anti-interference ability, the composition of the reagent R is as follows:

[0041] Reagent R contains:

[0042] CAPS buffer (pH7.0) 50mmol / L

[0043] 4-aminoantipyrine 1.0mmo1 / L

[0044] Cholesterol esterase (CHE) 2000U / L

[0045] Cholesterol oxidase (CHO) 2000U / L

[0046] Peroxidase (POD) 2500U / L

[0047] Sodium 3,5-dichloro-2-hydroxybenzenesulfonate (DHBS) 3.5mmol / L

[0048] Magnesium chloride 2.5mmol / L

[0049] Trehalose 20g / L

[0050] Mannitol 20g / L

[0051] Hydroxyethylidene diphosphonic acid 2ml / L

[0052] Octadecyltrimethylammonium bromide · 0.3g / L

[0053] EMULGEN-7070.35ml / L

[0054] Ascorbate oxidase 2.5KU / L,

[0055] Bilirubin oxidase 2KU / L

[0056] Preservative 0.5ml / L

[0057] 2) Detection usage method:

[0058] The total cholesterol detection reagent described in this example is used in an automatic biochemical analyzer, such as a Hitachi 7180 automatic analyzer, and is ...

Embodiment 2

[0063] Correlation experiment: using the formula in Example 1 to prepare reagents, and conducting a control test with the total cholesterol kit of a company approved by the State Food and Drug Administration, which is common in the market, and testing 20 clinical serum samples at the same time, the test results are shown in Table 2 Show. And obtained the correlation curve of the two reagents (such as figure 1 shown).

[0064] Table 2 Example 1 reagent and market common and recognized total cholesterol assay kit comparative detection results

[0065]

[0066] The test results show that the correlation coefficient of the two kits is 0.9997, which shows that the two have a great correlation, and the stabilizer and anti-interference agent added in the reagent of Example 1 do not affect the accuracy of the reagent. Show that the reagent of embodiment 1 has good accuracy.

Embodiment 3

[0068]Interference test: take fresh mixed serum, divide it into 2 equal parts, and then divide each equal part into 5 equal parts, add different interfering substances, so that the concentration in the serum reaches the requirements in Table 3. Then respectively use Example 1 and the control reagent (same as Example 2) to simultaneously measure the content of total cholesterol in serum by comparison, and the measurement results of each group after adding different interfering substances are shown in Table 3. Relative deviation (%) = (measuring mean value of interfering samples - measuring mean value of samples without interfering substances) / measuring mean value without interfering substances × 100%.

[0069] Table 3 embodiment reagent anti-interference performance comparison

[0070]

[0071] It can be seen from Table 3 that the reagent of Example 1 has no obvious interference on the test results when ascorbic acid≤2000μmol / L, bilirubin≤986μmol / L, and hemoglobin≤10.5g / L....

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Abstract

The invention provides a total cholesterol detection reagent with high accuracy and disturbance resisting capacity and relates to the technical field of total cholesterol detection. A biological buffer CAPS (3-(hexanaphthene amino)-1-propanesulfonic acid) is used in the reagent R, and therefore the buffering ability is ensured; a surfactant octadecy trimethyl ammonium bromide and a surfactant EMULGEN-707 are added into the reagent R, the emulsification effect of the reagent is improved well, and the accuracy and specificity of the reagent are improved; stabilizers such as trehalose and mannitol are added into the reagent R, and the stability of the reagent can be improved effectively; hydroxy ethidene diphosphonic acid is added into the reagent R, heavy metal ions can be effectively chelated, and the accuracy of the reagent can be improved; chemical-resistant succinoxidase and bilrubin oxidase are added into the reagent, disturbance of ascorbic acid and bilirubin can be effectively removed, and the reagent is simple in preparation, low in price and very suitable for large-area clinical popularization.

Description

technical field [0001] The invention relates to the technical field of total cholesterol detection, in particular to a total cholesterol detection reagent with high accuracy and strong anti-interference ability. Background technique [0002] Total cholesterol refers to the sum of cholesterol contained in all lipoproteins in the blood. The total cholesterol level of the population mainly depends on genetic factors and lifestyle. Total cholesterol includes free cholesterol and cholesteryl esters, and the liver is the main organ for synthesis and storage. Cholesterol is an important raw material for the synthesis of adrenal cortex hormones, sex hormones, bile acids and vitamin D and other physiologically active substances, and is also the main component of cell membranes. Cholesterol (CHO) and cholesteryl ester (ChE) are lipids, which are fats that exist in the blood. a form of Cholesterol has many important functions in the body, and its main functions are as follows: chole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60C12Q1/44C12Q1/28C12Q1/26
Inventor 李静高雪萍谭柏清
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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