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Composition of probiotic fermented horseradish tree leaves as well as preparation method and application of composition

A technology of Moringa leaves and probiotics, applied in the direction of bacteria used in food preparation, application, food preparation, etc., can solve the problems of few Moringa leaves, cumbersome process, and low utilization rate of Moringa leaves

Pending Publication Date: 2015-12-30
SANZHU FUER PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are very few technologies about probiotic fermentation of Moringa leaves. The relevant patent (patent application number: 201410018407.7) involves the general technology of probiotic fermentation of Moringa leaf extract, but it needs to extract a large amount of Moringa leaves before combining with other medicinal materials. Combining fermentation, and finally separating the supernatant, the process is relatively cumbersome, and the utilization rate of Moringa leaves is low

Method used

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  • Composition of probiotic fermented horseradish tree leaves as well as preparation method and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Preparation of composition A of probiotic fermented Moringa oleifera leaves

[0030] Take 30g of dried Moringa leaves, crush them through a 50-mesh sieve, soak in 30 times of water for 30 minutes, add glucose 0.7g / 100ml, peptone 0.4g / 100ml, yeast extract 0.13g / 100ml, yeast powder 0.07g / 100ml, inorganic salt (K 2 HPO 4 ·3H 2 O0.1g / 100ml, KH 2 PO 4 0.1g / 100ml, ferrous sulfate 7H 2 O5.0mg / 100ml, zinc sulfate 7H 2 O4.4mg / 100ml, magnesium sulfate 7H 2O50mg / 100ml), sucrose 3.0g / 100ml and sucralose 0.008g / 100ml. Adjust the pH value to about 7.0, and sterilize at about 115° C. for 50 minutes to obtain a sterile extract of Moringa oleifera leaves. When the temperature of the extract drops to about 39°C, insert the pre-cultured Bifidobacterium bifidum (isolated from normal human body, identified by the Institute of Microbiology, Chinese Academy of Sciences) and Bifidobacterium breve (AS1.2213) bacteria solution, the inoculation amount Both are 0.5% (v / v). ...

Embodiment 2

[0031] Embodiment 2: the preparation of the composition B of probiotic fermented Moringa leaf

[0032] Take 40g of dried Moringa leaves, crush them through a 65-mesh sieve, soak in 35 times of water for 30 minutes, add 0.7g / 100ml of glucose, 0.4g / 100ml of peptone, 0.13g / 100ml of yeast extract, and 0.07g / 100ml, inorganic salt (K 2 HPO 4 ·3H 2 O0.1g / 100ml, KH 2 PO 4 0.1g / 100ml, ferrous sulfate 7H 2 O5.0mg / 100ml, zinc sulfate 7H 2 O4.4mg / 100ml, magnesium sulfate 7H 2 O50mg / 100ml). Adjust the pH value to about 7.0, and sterilize at about 115° C. for 50 minutes to obtain a sterile extract of Moringa oleifera leaves. When the temperature of the extract drops to about 39°C, insert the pre-cultured Bifidobacterium bifidum (isolated from normal human body, identified by the Institute of Microbiology, Chinese Academy of Sciences) and Bifidobacterium infantis (AS1.853) bacteria solution, the inoculation amount Both are 0.5% (v / v). After 6 hours of fermentation at 37°C, the pre...

Embodiment 3

[0033] Example 3: Preparation of Composition C of Probiotic Fermented Moringa Leaf

[0034] Take 50g of dried Moringa leaves, crush them through an 80-mesh sieve, soak them in 40 times of water for 30 minutes, add 0.7g / 100ml of glucose, 0.4g / 100ml of peptone, 0.13g / 100ml of yeast extract, and 0.07g / 100ml of yeast powder to the soaking liquid. 100ml, inorganic salt (K 2 HPO 4 ·3H 2 O0.1g / 100ml, KH 2 PO 4 0.1g / 100ml, ferrous sulfate 7H 2 O5.0mg / 100ml, zinc sulfate 7H 2 O4.4mg / 100ml, magnesium sulfate 7H 2 O50mg / 100ml), sucrose 3.0g / 100ml and sucralose 0.008g / 100ml. Adjust the pH value to about 7.0, and sterilize at about 115° C. for 50 minutes to obtain a sterile Moringa oleifera leaf extract. When the temperature of the extract drops to around 39°C, insert the pre-cultivated Bifidobacterium adolescent (AS1.2190) and Bifidobacterium breve (AS1.2213) bacteria solution, the inoculation amount is 0.5% (volume / volume) . After 7 hours of fermentation, the pre-cultured Lacto...

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Abstract

The invention discloses a composition of probiotic fermented horseradish tree leaves as well as a preparation method and application of the composition. The preparation method comprises the following steps: crushing and immersing horseradish tree leaves; adding glucose, peptone, yeast extract, yeast powder and inorganic salt into an immersing solution; extracting at a high pressure; and after carrying out probiotic fermentation and centrifuging, adding polydextrose and fructo-oligosaccharide syrup and sterilizing to obtain the composition of the probiotic fermented horseradish tree leaves. The composition can be used for preparing health products, foods and beverages capable of lowering blood fat and improving a sleeping function. An experiment shows that the composition of the probiotic fermented horseradish tree leaves can be used for reducing the contents of total cholesterol (TC) and triglyceride (TG) in blood serum, and improving the content of high density lipoprotein cholesterol (HDL-C) in the blood serum; the composition of the probiotic fermented horseradish tree leaves, disclosed by the invention, has a function of lowering a blood fat level.

Description

technical field [0001] The invention relates to a composition of fermented leaves of Moringa oleifera with multiple strains of intestinal probiotics and a preparation method thereof, as well as its application in reducing blood fat and improving sleep function, and belongs to the technical field of functional drinks. Background technique [0002] Moringa is a high-calcium plant product. The calcium content is more than 4 times that of milk. In addition, it also contains high potassium, iron, zinc and other trace elements, and contains protein and amino acids similar to soybeans. A functional food containing complete nutrients, a person only needs to eat about 5 grams per day to supply the nutritional needs of the human body for a day. Moringa leaf powder is rich in nutrition and comprehensive, vitamin E is 70 times and 40 times that of spirulina and soybean powder respectively. The vitamin C contained in 100 grams of Moringa leaves is 7 times that of citrus, iron is 3 times...

Claims

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Application Information

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IPC IPC(8): A23L2/38A23L1/30
CPCA23L2/382A23V2002/00A23V2400/137A23V2400/125A23V2400/513A23V2400/529A23V2400/517A23V2400/519A23V2400/175A23V2400/113A23V2400/169A23V2200/3202A23V2200/3262A23V2250/21A23V2250/218
Inventor 吴炳新孙筱林褚新红甄宗武陆继臣吕美
Owner SANZHU FUER PHARMA
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