A biogenic
nanoparticle synthesis system for the production of
chitosan-
copper nanoparticles using
plant extract, wherein the
system comprises the following: a sealed reaction vessel made of
corrosion-resistant material, forming an inner
reaction chamber configured to contain an aqueous
copper salt solution and a
plant extract mixture; a
temperature control unit that is thermally coupled to the reaction vessel and configured to maintain the temperature inside the
reaction chamber within a predetermined range between 25 degrees Celsius and 75 degrees Celsius with a tolerance of plus / minus one
degree Celsius; a stirring unit consisting of a variable speed motor and a stirring shaft extending into the inner
reaction chamber, wherein the stirring unit is configured to ensure uniform mixing at speeds between one hundred and one thousand
revolutions per minute; a
plant extract introduction unit consisting of a metered inlet line and a flow control valve configured to introduce an
aqueous extract from
Moringa oleifera leaves into the reaction chamber at a controlled rate to initiate the reduction of
copper ions; a dosing unit for
chitosan solution, consisting of a programmable
peristaltic pump connected to a reservoir containing a
chitosan solution dissolved in dilute
acetic acid, wherein the dosing unit is configured to dispense the chitosan solution in a controlled dropwise manner into the reaction chamber; a
pH monitoring unit with a
pH probe protruding into the reaction chamber; a base addition unit consisting of a
reagent reservoir with
sodium hydroxide solution and a precision dosing pump to maintain a pH value of the reaction mixture of six plus / minus zero point five; a
control unit that is operationally connected to the
temperature control unit, the stirring unit, the plant extract feeding unit, the chitosan solution dosing unit, the
pH monitoring unit and the base addition unit, wherein the
control unit is configured to synchronize the
temperature control, the
reagent addition rate, the stirring speed and the pH correction to produce a stable colloidal suspension of chitosan copper nanoparticles.