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Preparation method and use of moringa oleifera leaf polysaccharides

A technology of moringa leaf and moringa leaf powder, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, organic active ingredients, etc., can solve the problems of long extraction time and low extraction rate, and achieve short extraction time and high extraction efficiency. The effect of high extraction rate and low extraction temperature

Inactive Publication Date: 2015-08-12
隽觅(广州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the defects of longer extraction time and low extraction rate in the extraction process of Moringa leaf polysaccharide in the prior art, the purpose of the present invention is to provide a preparation method and application thereof of Moringa leaf polysaccharide to solve the above defects

Method used

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  • Preparation method and use of moringa oleifera leaf polysaccharides
  • Preparation method and use of moringa oleifera leaf polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, Preparation of Moringa Leaf Polysaccharide

[0035] (1) Material preparation: Weigh 1 kg of Moringa leaves, dry them at 50°C for 2 hours, crush them, and pass through a 40-mesh sieve to obtain Moringa leaves powder;

[0036] (2) Extraction: Take the Moringa oleifera leaf powder obtained in step (1), add distilled water 20 times the volume of the Moringa oleifera leaf powder, treat it under a microwave with a power of 500W for 6 minutes, and place it in a warm water bath at 45°C with an ultrasonic wave with a power of 300W. Extract 2 times, each treatment for 30 minutes, filter, combine the filtrate, concentrate the filtrate to 1 / 3 of the original volume under reduced pressure, centrifuge at 14 ° C for 10 min in a centrifuge with a rotation speed of 10,000 r / min, and take the supernatant, Concentrate the supernatant to 1 / 4 of the original volume to obtain a concentrate;

[0037](3) Deproteinization: add 2mol / L hydrochloric acid to the concentrated soluti...

Embodiment 2

[0040] Embodiment 2, Preparation of Moringa Leaf Polysaccharide

[0041] (1) Material preparation: weigh 1 kg of Moringa oleifera leaves, dry at 50°C for 2.5 h, crush them, and pass through a 45-mesh sieve to obtain Moringa oleifera leaf powder;

[0042] (2) Extraction: Take the Moringa oleifera leaf powder obtained in step (1), add distilled water 25 times the volume of the Moringa oleifera leaf powder, process it under a microwave with a power of 500W for 7 minutes, and place it in a warm water bath at 50°C with an ultrasonic wave with a power of 300W. Extract 2 times, each treatment for 35 minutes, filter, combine the filtrate, concentrate the filtrate to 1 / 3 of the original volume under reduced pressure, centrifuge at 14°C for 10 min in a centrifuge with a rotation speed of 10000 r / min, and take the supernatant, Concentrate the supernatant to 1 / 4 of the original volume to obtain a concentrate;

[0043] (3) Deproteinization: add 2mol / L hydrochloric acid to the concentrat...

Embodiment 3

[0046] Embodiment 3, Preparation of Moringa Leaf Polysaccharide

[0047] (1) Material preparation: weigh 1 kg of Moringa oleifera leaves, dry at 50°C for 3 h, pulverize, and pass through a 50-mesh sieve to obtain Moringa oleifera leaf powder;

[0048] (2) Extraction: Take the Moringa oleifera leaf powder obtained in step (1), add distilled water 25 times the volume of the Moringa oleifera leaf powder, treat it under a microwave with a power of 500W for 8 minutes, and place it in a warm water bath at 55°C with an ultrasonic wave with a power of 300W. Extract 3 times, each treatment for 40 min, filter, combine the filtrate, concentrate the filtrate to 1 / 3 of the original volume under reduced pressure, centrifuge at 14°C for 10 min in a centrifuge with a rotation speed of 10000 r / min, and take the supernatant, Concentrate the supernatant to 1 / 4 of the original volume to obtain a concentrate;

[0049] (3) Deproteinization: add 2mol / L hydrochloric acid to the concentrated soluti...

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Abstract

The invention belongs to the chemical field of natural products and particularly relates to a preparation method and use of moringa oleifera leaf polysaccharides. The preparation method comprises the steps of carrying out combined microwave-ultrasound extraction, hydrochloric acid method deproteinization, hydrogen peroxide method decoloration and AB-8 macroporous adsorption resin column separation, so as to prepare the moringa oleifera leaf polysaccharides. The preparation method of the moringa oleifera leaf polysaccharides has the advantages of short extraction time, low extraction temperature, low energy consumption and high extraction rate and purity. Besides, an animal experiment proves that the prepared moringa oleifera leaf polysaccharides have remarkable treatment effect to hyperuricosuria; by decreasing the content of cholesterol, triacylglycerol, urea nitrogen, creatinine and xanthine oxidase, the production of uric acid is achieved; meanwhile, by directly decomposing uric acid, renal functions are improved, the excretion of uric acid is promoted, and blood vessels are protected, thereby being favorable to the recovery of patients with the hyperuricosuria. The preparation method has a wide medical application prospect.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and in particular relates to a preparation method and application of Moringa oleifera leaf polysaccharide. Background technique [0002] Moringa oleifera (Moringa oleifera Lam.) is a plant of the genus Moringa in the Moringaceae family. It is a perennial tropical deciduous tree widely planted in tropical and subtropical regions of Asia and Africa, and has strong adaptability to soil conditions and rainfall. It is also cultivated in Guangdong, Taiwan and other places in my country. Moringa can be used for viewing, and its roots, leaves and tender fruits are edible; its seeds can be squeezed for oil, with an oil content of about 30%, which has high economic value. It has been found through research that, in addition to rich nutritional value, Moringa also exhibits good activities in anti-oxidation, hypoglycemic, hypolipidemic, and antifungal aspects, and the polysaccharides and polysacchari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P19/06
Inventor 严俊霖萧丽雅
Owner 隽觅(广州)生物科技有限公司
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