126 results about "Natural Products Chemistry" patented technology

The invention relates to a method for extracting rose damascana and rose flower water from roses, belonging to the technical field of natural product chemistry. In the invention, the picked fresh roses and saline solution are placed into a pulp refiner with a certain ratio of material to liquid for homogenization and crushing after the stamens of the roses and impurities are removed, the sample is poured into a distillation still for dipping, a vent valve is opened, water steam flow rate is adjusted to carry out steam distillation, the temperature of a condenser is adjusted, oil-water mixed steam is condensed, the flowing-out liquid is collected until the volume of the flowing-out liquid and the weight of rose leaves form a certain proportion, the flowing-out liquid is placed still and layered at low temperature, rose damascana dehydrating agents are added in an upper layer of oil, and then the rose damascana is obtained after being dehydrated; the solution at the lower layer is the rose flower water. Compared with the prior art, the method has the advantages of little loss of top note of the extracted rose damascana, high product quality, simple operation and low production cost.

The invention belongs to the chemical field of natural products and particularly relates to a preparation method and use of moringa oleifera leaf polysaccharides. The preparation method comprises the steps of carrying out combined microwave-ultrasound extraction, hydrochloric acid method deproteinization, hydrogen peroxide method decoloration and AB-8 macroporous adsorption resin column separation, so as to prepare the moringa oleifera leaf polysaccharides. The preparation method of the moringa oleifera leaf polysaccharides has the advantages of short extraction time, low extraction temperature, low energy consumption and high extraction rate and purity. Besides, an animal experiment proves that the prepared moringa oleifera leaf polysaccharides have remarkable treatment effect to hyperuricosuria; by decreasing the content of cholesterol, triacylglycerol, urea nitrogen, creatinine and xanthine oxidase, the production of uric acid is achieved; meanwhile, by directly decomposing uric acid, renal functions are improved, the excretion of uric acid is promoted, and blood vessels are protected, thereby being favorable to the recovery of patients with the hyperuricosuria. The preparation method has a wide medical application prospect.

The invention provides a method for extracting rubusoside from sweet tea leaves, belonging to the technical field of natural product chemistry. The method comprises the following steps: drying the sweet tea leaves; extracting with ethanol solution, filtering, carrying out pressure reduced concentrating, then absorbing with resin, eluting with ethanol; carrying out pressure reduced concentrating on the elution solution, decoloring, crystallizing and recrystallizing to prepare into the pure rubusoside product. The processes for crystallizing, recrystallizing and purifying rubusoside are simple and easy to operate and ensure high purification degree.

The invention relates to a method for extracting anthocyanin from purple potatoes, which comprises the following steps of: safely and innocuously extracting anthocyanin crude extract with citric acid acidified ethanol from purple potatoes, and separating and purifying purple potato anthocyanin with AB-8 macropore adsorbent resin. With the method, anthocyanin as a main active ingredient in purple potatoes is extracted, separated and purified by adopting a natural product chemistry and Chinese traditional medicine modern production technology and made into purple potato anthocyanin extract of which the purity is 10-15%. The invention realizes high-ratio utilization of potato resources and industrial production of series products safely, effectively, simply, reasonably and economically and sufficiently embodies the idea of green chemical production.

The invention relates to a method for extracting plant flavone compounds by an enzymatic process, which comprises the following steps: (1) lixiviating, wherein enzymolysis water extraction is carried out once, and alcohol extraction is carried out twice; (2) purifying on the columns; (3) and processing the analytic solution to obtain the plant flavone compound product. Under the guidance of biotechnology theory, natural product chemical theory and technology and international drug test standard, the invention formulates an experimental scheme for production, and determines proper enzymatic process extraction technique and flavone glycoside biotransformation technique, thereby obtaining the product with high biological value. The invention determines to compound multiple enzyme preparations, optimizes the optimal transformation conditions and enhances the flavone biotransformation efficiency. The invention formulates a technical scheme of adsorption with macroporous absorbent resin to be used in industrial production, thereby increasing the content of aglycon flavones in the product.

The invention belongs to the chemistry of natural product field, which more particularly relates to a preparation technique of tetrandrine alkaloid. Dilute acid solution is used for percolating tetrandrine meal, leachate is absorbed by macroporous resin column, after being washed, alcohol solution with ammonia / amine is used for eluting, and then total alkaloids is get by concentration, finally tetrandrine alkaloid with high purity is obtained by crystallization. The tetrandrine alkaloid has the advantages of easy operation, less hazardous waste and residue, low cost, high yield, and getting all the tetrandrine alkaloid for once, etc. The method is a simple, economic, fast and strong practical processing method which comprehensively utilizes the tetrandrine alkaloid.

The invention provides a method for preparing high-purity epigallocatechin (EGC) from a tea polyphenol rough product, belonging to the technical field of natural product chemistry. According to the method, the tea polyphenol rough product is used as a raw material and is subjected to macroporous resin adsorption, low-concentration aliphatic alcohol elution, concentration, crystallization and drying to obtain the EGC with content of more than 90 percent. The method has the advantages of high content of the EGC obtained by the preparation process, easiness and convenience for operation, low production cost, contribution to industrial production and capability of meeting the market requirements in the fields of modern functional foods, health care products and the like.

The invention discloses a method for extracting high-purity theanine from fresh tea leaves, and belongs to the technical field of chemistry of natural products. The method includes constant-temperature digestion, centrifugation, primary membrane concentration, solvent extraction, electrodialysis, membrane dialysis, secondary membrane concentration, vacuum concentration, ethanol extraction, crystallization and microwave drying. The method has the advantages that the method is substituted for conventional methods based on ion exchange adsorption, substantial reagents needed by elution of conventional techniques are reduced due to technological adoption of electrodialysis, and operation equipment is easy to acquire, so that the method is simple to operate, low in production cost and ecologically environment-friendly; experiments prove that the purity of products obtained according to the method can be more than 98% and separation efficiency is high.

The invention discloses a method for extracting low-caffeine high-content tea polyphenol from fresh tea, belonging to the technical field of chemistry of natural products. The method comprises the following concrete steps: S1, extraction; S2, primary evaporative concentration; S3, water precipitation; S4, CF filtration; S5, resin adsorption; S6, membrane concentration; S7, secondary evaporative concentration; S8, spray drying; and S9, deironing. According to the invention, fresh tea is used as a raw material, and the disadvantages of easy pasting and uneasy filtration during extraction of crushed and sieved dry tea at a high temperature in the prior art is overcome; purified water is used as a medium for direct separation of low-caffeine high-content tea polyphenol, and leaching of a plurality of fat-soluble pesticides is reduced; the product prepared by using the method has caffeine content of lower than 1% and tea polyphenol content of 99.8%. The method has the advantages of simple operation, high separation efficiency, low cost and a short production period and is suitable for industrialized large-scale production.

The invention belongs to the chemical field of natural products and in particular relates to an extraction method and an application of moringa oleifera leaf total flavones. The extraction method adopts microwave and ultrasound to synergistically extract the moringa oleifera leaf total flavones, and has the advantages of high efficiency, time saving, energy conservation, low energy consumption, simple operation and good repeatability. Besides, an animal test proves that the extracted moringa oleifera leaf total flavones have a significant treatment effect on hyperuricosuria and Alzheimer's disease. The total flavones provided by the invention can reduce uric acid, and can also reduce cholesterol, triacylglycerol, urea nitrogen, creatinine and xanthine oxidase, reduce the generation of uric acid, directly decompose uric acid, improve the renal function, promote uric acid excretion, and protect multiple levels of blood vessels to reduce uric acid, so that the moringa oleifera leaf total flavones are beneficial for healing of lithemia patients and have a broad medical application prospect.

The invention relates to a multi-substituent-group naphthalene compound with antibacterial activity in aloe as well as a preparation method and an application of the naphthalene compound and belongs to the chemical technical field of natural products. The structural formula of the compound is shown in formula (I) in the description. The compound is prepared from traditional edible and medicinal plant aloe as a raw material through extraction, silica gel column chromatography and high-pressure liquid-phase chromatographic separation and purification. Activity tests prove that the compound has a better antibacterial effect and can effectively inhibit growth of microorganisms in a feed liquid and prolong the expiration date of the tobacco feed liquid after being added to the tobacco feed liquid and has good application prospects.

The invention belongs to the field of natural product chemistry, and relates to a method for preparing 1-deoxynojirimycin by a membrane separation technology. The method comprises the following steps of collecting fresh mulberry leaves, drying, crushing, adding deionized water into the crushed mulberry leaves for soaking, adding the soak into a nanofiltration membrane system with filtration to carry out filtration, adding the filtrate into a reverse osmosis membrane to carry out concentration, adding ethyl acetate into the concentrated filtrate to carry out extraction, carrying out pressure-reducing concentration of an aqueous phase obtained by the previous step, and carrying out vacuum drying to obtain the desired product. The method for preparing 1-deoxynojirimycin by a membrane separation technology has the advantages of simple process, good operability, low energy consumption, less pollution and good applicability for industrialized production.

The invention belongs to the field of natural product chemistry, and relates to extraction, separation and purification of natural products, in particular to a technical method for extracting high-purity total alkaloids from dyers woad leaf. The method for preparing indigo and indirubin from dyers woad leaf is mainly characterized by comprising the following steps of: drying, pulverizing and sieving dyers woad leaves; heating and extracting by reflux by an ethanol solution containing sodium hydroxide; recovering ethanol from filtrate; concentrating the filtrate, regulating the pH to be neutral by a hydrochloric acid solution, centrifuging; dissolving the precipitate by the hydrochloric acid solution, and centrifuging to obtain supernatant for later use; performing microfiltration on the supernatant in membrane separation equipment; loading the stock solution after microfiltration on a resin column for absorption, then eluting with water to remove water soluble impurities, discarding the water eluent; eluting with aqueous ethanol, collecting the eluent; and recovering ethanol from the eluent, concentrating and drying to obtain a dark red product. The method has the advantages of low production cost of the products, simple process route, smaller toxicity of the extraction solvent and short extraction cycle, and is suitable for application in industrial production. Based on the advantages, the method can further promote industrialized development of the dyers woad leaf extract as a multifunctional product such as a medicine, a health product and the like.

Belonging to the field of chemical analyses on natural products, the present invention relates to a method for extracting, separating, purifying and testing natural products, in particular to a technique for extracting swainsonine by continuous column chromatography. The present invention resolves the defects of the prior art, including a complex process, high cost and low yield rate. The solution orderly includes the following steps: (1) the preprocessing of locoweed; (2) the acquisition of locoweed extract; (3) the preparation of a crude product: The extract is roughly separated by polar macroporous adsorption resin and, after TLC test, merged with eluant containing swainsonine to serve as the crude product for later use; (4) the purification of the swainsonine: The crude product is purified by silica gel column chromatography, and by the TLC test and the GC test, the parts of the swainsonine, which have relative content larger than or equal to 10 percent, are respectively collected; (5) the acquisition of a pure swainsonine product by the HPLC test; the parts collected in the step (4) undergo the HPLC test in order to collect eluate in the appearance time period of the swainsonine, and after the solvent is evaporated, the pure swainsonine product can be produced by recrystallization.

The invention relates to a method for extracting solanine from potato peels. The method comprises the following steps of: safely and innocuously extracting a crude solanine extract from potato peels by using ethanol with the assistance of ultrasonic wave; and separating and purifying the crude solanine extract by using macroporous absorption resin to obtain a potato solanine extract. The method can be used for safely, effectively and industrially producing the potato solanine extract with a purity specification of 20-35 percent to realize the high-valued utilization of potato resources and the industrial production of serial products. Moreover, the solanine which is a main active constituent of the potatoes is extracted, separated and purified by adopting a chemical technology of natural products and a modernized production technology of traditional Chinese medicine, which sufficiently embodies a green chemical production concept.

The invention discloses a method for extracting monomeric EGCG from fresh tea, belonging to the technical field of chemistry of natural products. The method comprises the following concrete steps: S1, soaking; S2, centrifugation; S3, primary adsorption; S4, MVR concentration; S5, CF filtration; S6, secondary adsorption; S7, concentration; S8, rinsing; S9, dehydration; S10, drying; and S11, deironing. According to the invention, fresh tea is used as a raw material; the disadvantages of easy pasting and uneasy filtration during extraction of crushed and sieved dry tea at a high temperature in the prior art is overcome; purified water is used as a medium for direct separation of EGCG monomer, and leaching of a plurality of fat-soluble pesticides is reduced; in the process of purification, an LX-8 resin is used for adsorbing EGCG, and an HZ818 resin is used for adsorbing caffeine; crystallization, rinsing and drying are carried out so as to obtain the EGCG monomer; the obtained EGCG monomer is off-white or white powder, and yield of the monomer reaches about 99%. The method has the advantages of simple operation, high separation efficiency, low cost, a short production period and high product purity and is suitable for industrialized large-scale production.

The invention discloses a dihydroagarofuran type sesquiterpene compound, the preparing method of the compound, and the application of the compound in preparing anti-microbial pesticides and relates to the field of medicinal chemistry. According to the application of the novel sesquiterpene compound in anti-microbial pesticides, the structure of the adopted sesquiterpene compound is shown in the figure of the abstract and is named 1beta-acetyl-6alpha-(5-carboxyl-N-methyl-2-pyridone)-8beta-nicotinoyl-9alpha-benzoyl-beta-dihydroagarofuran. The compound is characterized in that the stem leaves of the plant Monimopetalum Chinese Rehd. are used as raw materials, and then the compound is obtained through extraction and separation with the natural product chemistry method. As a pesticide, the compound has a remarkable resisting effect on pests including plutella xylostella, cabbage caterpillar, corn armyworm, prodenia litura, argyrogramma agnate, pea aphids, rice-stem borer, rice tryporyza incertulas and henosepilachna vigintioctomaculata. As a sterilant, the compound has a remarkable resisting effect on sclerotinia sclerotioru, rhizoctonia cereal, fusarium graminearum and botrytis cinerea person.

The invention discloses a method for extracting theanine from fresh tea, belonging to the technical field of natural product chemistry. The method comprises specific steps of S1. soaking; S2. centrifuging; S3. concentrating for the first time; S4. adsorbing for the first time; S5. adsorbing for the second time; S6. adsorbing for the third time; S7. concentrating for the second time; S8. concentrating for the third time; S9. disinfecting; S10. spray-drying; S11. deironing. The method adopts fresh tea as the raw material, thus overcoming the defects in the traditional technology that smashed and sieved dry tea is easy to be burnt and difficult to filter in extraction at high temperature; purified water is used as a medium for directly separating theanine, so that less fat soluble pesticide is leached; the method adopts three times of resin adsorption for respectively adsorbing EGCG (epigallocatechin gllate), caffeine and theanine in the filtrate, and finally natural theanine with content of 30-45% can be separated. The method has advantages of simple operation, high separation efficiency, low cost, short production cycle, high purity of the product, and is applicable to large scale factory production.

The invention discloses anti-inflammatory application and an extraction method of 6-hydroxyl-eremophilane-7(11)-en-12,8-lactone in ligularia sibirica, and belongs to the technical field of chemistry of natural products. 6-hydroxyl-eremophilane-7(11)-en-12,8-lactone has an anti-inflammatory effect, thereby being applied to preparation of an anti-inflammatory drug. The extraction method comprises the following steps: 1) taking coarse ligularia sibirica medicinal material powder, and performing cold leaching for extraction with ethanol with the concentration of 95% to obtain an ethanol extract; 2) performing Soxhlet extraction on the obtained extract with petroleum ether (60-90 DEG C) at first, then performing Soxhlet extraction with dichloromethane, and recovering a solvent to obtain a dichloromethane extract; 3) separating the dichloromethane extract through silica gel column chromatography, collecting a petroleum ether (60-90 DEG C)-ethyl acetate eluant, wherein the ratio of petroleum ether (60-90 DEG C) to ethyl acetate is 6:1, carrying out stilling till brown yellow precipitations are separated out, and carrying out refining to obtain a white acicular crystal to be identified as 6-hydroxyl-eremophilane-7(11)-en-12,8-lactone. The extraction method has the advantages of being mild in extraction condition, simple and convenient to operate, high in extraction rate, and low in cost.

Belonging to the technical field of natural product chemistry, the invention relates to an isoflavone compound with antioxidant activity, a preparation method and application thereof. The isoflavone compound is separated from the traditional Chinese medicinal material kudzuvine root, and is named as: 4', 8-dihydroxy-7-hydroxymethyl-6-methoxy-isoflavone, and the molecular formula is C17H14O6. Antioxidant activity experiments are carried out on the compound, and the result shows that the compound has strong antioxidant activity. At the same time, the compound can be used as a tobacco feed liquid additive, and can effectively prevent rancidity of tobacco feed liquid and prolong the shelf life.

The invention discloses a method for extracting high-content tea polyphenol from fresh tea, belonging to the technical field of chemistry of natural products. The method comprises the following concrete steps: S1, extraction; S2, primary evaporative concentration; S3, water precipitation; S4, CF filtration; S5, evaporative concentration; S6, spray drying; and S7, deironing. According to the invention, fresh tea is used as a raw material, and the disadvantages of easy pasting and uneasy filtration during extraction of crushed and sieved dry tea at a high temperature in the prior art is overcome; ethyl acetate is used as a medium for direct separation of high-content tea polyphenol, and leaching of a plurality of water-soluble pesticides is reduced; the high-content tea polyphenol product prepared by using the method has tea polyphenol content of as high as 84.2% and caffeine content of 9.6%, and the product has a slightly bright color and high solution clarity. The method has the advantages of simple operation, high separation efficiency, low cost, a short production period and high product purity and is suitable for industrialized large-scale production.

The invention discloses a new Kosteletzkya virginica (L.) Presl. sesquiterpene compound, and a preparation method and a use thereof, and belongs to the chemical field of natural products. The above new cadinane type sesquiterpene compound is obtained by extracting and separating from a raw material the rhizome of a halophyte Kosteletzkya virginica (L.) Presl. through chemical processes of the natural products. The structural formula of the compound is shown in the specification, is named as virginicin, has a strong killing effect on Verticillium dahliae, and can be used for controlling cotton verticillium wilt.

The invention provides a preparation method for extracting swertiamarin from Swertia bimaculata, belonging to the technical field of natural product chemistry. The preparation method comprises: extracting dried Swertia bimaculata with an ethanol solution, filtering, concentrating under a pressure reduced condition, carrying out resin adsorption, eluting with ethanol, concentrating the eluate under a pressure reduced condition, decolorizing, and crystallizing and recrystallizing to obtain high-purity swertiamarin. Crystallization and recrystallization purifying processes of the swertiamarin are simple and easy to operate and have high purification degree.

The invention relates to an antibacterial isoflavonoid compound, and a preparation method and application thereof, belonging to the technical field of the chemistry of natural products. The compound is named as 8-hydroxy-7-hydroxymethyl-4',6-dimethoxy-isoflavone. Results of activity tests prove that the compound has good bacteriostatic effect. When applied to tobacco liquid for quality preservation, the compound can effectively inhibit the growth of microbes in the tobacco liquid and prolongs the shelf life of the tobacco liquid; and when applied to tipping paper of a cigarette, the compound can eliminate or reduce propagation and reproduction of bacteria in the tipping paper of the cigarette.

The invention belongs to the field of medicine and natural product chemistry, and particularly relates to a novel isoflavone glycoside compound and a preparation method and application thereof. The isoflavone glycoside compound has the following structure shown in the description, normal phase (silica gel) column chromatography separation is adopted, reverse (open ODS) column chromatography separation is adopted, and a compound 2'-hydroxyl daidzin is obtained through methanol recrystallization finally. The novel isoflavone glycoside compound can be used for preventing and treating various diseases caused by the fact that too many free radicals in the human body are produced, or the capacity of eliminating the free radicals is decreased. Please see the formula in the description.

The invention belongs to the chemical technology field of natural product, particularly relating to a pretreatment method of a propolis effective component raw material by supercritical extraction. The invention is realized by the following mode: freezing raw material rough propolis at the temperature of -18 DEG C for 6-24 hours; after being smashed, processing with 5-20 times of pretreatment solution; after standing, separating impurities on the upper layer, filtering other parts, and carrying out supercritical CO2 extraction after filter residues are dried. The pretreatment solution is one of the solutions or the mixture of a plurality of solutions of ethanol, glycol and water. The propolis raw material processed by the invention dose not block, does not adhere to extraction kettle wall and is easy to clean in the supercritical CO2 extraction process. The invention has simple operation and cheap solution, does not generate any side effect on products, reduces equipment cleaning time, energy consumption cost and the like and improves extraction efficiency.

The invention relates to a diphenyl ether compound with antibacterial activity in honeysuckle and its preparation method and application, belonging to the technical field of natural product chemistry. The structural formula of the compound is shown in formula (I):, formula (I); the compound is prepared from the traditional medicinal and edible homologous plant honeysuckle as raw material, and is obtained through extraction of extract, silica gel column chromatography and high pressure liquid chromatography separation and purification steps . The activity test shows that the compound of the present invention has good antibacterial effect, adding the compound of the present invention to the tobacco feed liquid can effectively inhibit the growth of microorganisms in the feed liquid, prolong the shelf life of the tobacco feed liquid, and has a good application prospect.

The invention discloses an ultrasonic-assisted method for extracting dihydroquercetin in sorghum bran and belongs to the technical field of natural product chemistry. According to the method, the sorghum bran is utilized as a raw material, and a pure dihydroquercetin product can be obtained by the following steps of ultrasonic-assisted extraction, suction filtration, decompressing concentration, low-temperature standing, centrifugation, separation / enrichment, elution, distillate liquid low-temperature standing, crystallization and recrystallization and drying. The ultrasonic-assisted method disclosed by the invention can be applied to industrial production of high-purity dihydroquercetin, the utilized raw material of the sorghum bran is low in cost and easy to obtain, the ultrasonic-assisted method has the advantages of simple technological process, convenience in operation, low cost and no toxic harmful reagent pollution, and the dihydroquercetin can be applied to bulk pharmaceutical chemicals of medicine.

The invention relates to the fields of natural product chemistry and pesticide science and in particular to a safe agent and a combination agent for damage of amide herbicides to cereal crops. The safe agent for damage of amide herbicides to cereal crops comprises a jasmonic acid ester compound. By adopting the safe agent, the jasmonic acid ester compound is applied to alleviation of damage of amide herbicides to cereal crops for a first time, crops can be relatively well prevented from damage of the amide herbicides, and a novel direction is made for development of herbicide safe agents.