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Application of moringa oleifera leaf polysaccharide in preparation of medicines and foods for preventing and treating alcoholic liver injuries

A technology of alcoholic liver injury and Moringa leaves, which is applied in the field of application of Moringa leaf polysaccharides in the preparation of drugs and food for the prevention and treatment of alcoholic liver injury, which can solve the problems of research and application without anti-alcoholic liver injury, and achieve Yield-enhancing effect

Active Publication Date: 2017-03-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves upon existing methods for extracting or purifying plant material from plants that have been used traditionally (such as citrus). It allows for more effective use of these materials without causing harmful side reactions during processing.

Problems solved by technology

This patented technical problem addressed in this patent describes how to provide new methods for reducing harmful substances associated with alcohock syndrome including butterfly stalk virus, copper oxides, iron defibrillators, calcium ion blockers, etc., particularly through traditional medicine therapy involving taking multiple types of herbs containing different chemical agents like ethosulfate, chlorhexadecyl sulfurane, nigresistinylic Acids, flavonoids, caffeinates, gamma lactic acid estrone supplements, proteinohealth products, and others.

Method used

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  • Application of moringa oleifera leaf polysaccharide in preparation of medicines and foods for preventing and treating alcoholic liver injuries
  • Application of moringa oleifera leaf polysaccharide in preparation of medicines and foods for preventing and treating alcoholic liver injuries
  • Application of moringa oleifera leaf polysaccharide in preparation of medicines and foods for preventing and treating alcoholic liver injuries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the preparation of Moringa leaf polysaccharide

[0037] (1) material preparation: Moringa leaves are dried, pulverized, and crossed an 80-mesh sieve to obtain Moringa leaves powder for subsequent use;

[0038] (2) Degreasing: get the leaf powder of Moringa oleifera obtained in the step (1), add sherwood oil 3 times the volume (g:mL) of the leaf powder of Moringa oleifera to reflux for degreasing 2 times, each 6h, filter, and dry the filter residue, spare;

[0039] (3) Extraction: take the dried filter residue obtained in step (2) and use water as a solvent, the ratio of solid to liquid is 1:15, extract at 60°C for 0.5h, and the number of extractions is 3 times, combine the water extracts, and centrifuge at 14000rpm , take the supernatant to obtain the crude polysaccharide solution of Moringa oleifera leaves;

[0040] (4) Alcohol precipitation: Concentrate the crude polysaccharide solution of Moringa oleifera leaves obtained in step (3) to 1 / 2-1 / 3, adjust...

Embodiment 2

[0043] Embodiment 2: the preparation of Moringa leaf polysaccharide

[0044] (1) material preparation: Moringa leaves are dried, pulverized, and crossed an 80-mesh sieve to obtain Moringa leaves powder for subsequent use;

[0045] (2) Degreasing: take the Moringa oleifera leaf powder in step (1), add sherwood oil 3 times the volume (g:mL) of the Moringa oleifera leaf powder quality, reflux and degrease twice, 6 hours each time, filter, and dry the filter residue for subsequent use ;

[0046] (3) Extraction: take the dry filter residue obtained in step (2) and use water as a solvent, the ratio of solid to liquid is 1:20, extract at 80° C. for 1 hour, and the number of extractions is 3 times, combine the water extracts, and centrifuge at 14000 rpm. Get the supernatant to obtain the crude polysaccharide solution of leaves of Moringa oleifera;

[0047] (4) Alcohol precipitation: Concentrate the crude polysaccharide solution of Moringa oleifera leaves obtained in step (3) to 1 / 2-...

Embodiment 3

[0050] Embodiment 3: the preparation of Moringa leaf polysaccharide

[0051] (1) material preparation: Moringa leaves are dried, pulverized, and crossed an 80-mesh sieve to obtain Moringa leaves powder for subsequent use;

[0052] (2) Degreasing: take the Moringa oleifera leaf powder in step (1), add sherwood oil 3 times the volume (g:mL) of the Moringa oleifera leaf powder quality, reflux and degrease twice, 6 hours each time, filter, and dry the filter residue for subsequent use ;

[0053] (3) Extraction: take the dry filter residue obtained in step (2) and use water as a solvent, the ratio of solid to liquid is 1:25, extract at 70°C for 1.5h, and the number of extractions is 3 times, combine the water extracts, and centrifuge at 14000rpm , take the supernatant to obtain the crude polysaccharide solution of Moringa oleifera leaves;

[0054] (4) Alcohol precipitation: Concentrate the crude polysaccharide solution of Moringa oleifera leaves obtained in step (3) to 1 / 2-1 / 3, a...

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Abstract

The invention belongs to the chemical field of natural products, and discloses application of moringa oleifera leaf polysaccharide in preparation of medicines and foods for preventing and treating alcoholic liver injuries. The moringa oleifera leaf polysaccharide is crudely extracted by adopting a water extraction and alcohol precipitation method, and in combination with process flows of 14000rpm centrifugation, deproteinization, AB-8 type macroporous resin column chromatography and the like, purification is carried out to obtain relatively pure moringa oleifera leaf polysaccharide. The moringa oleifera leaf polysaccharide has a significant protection protective effect on liver injuries, and animal experiments prove that the moringa oleifera leaf polysaccharide has an effect of preventing and treating acute and subacute alcoholic liver injuries. The moringa oleifera leaf polysaccharide can be well applied to development of medicines and foods for preventing and treating the alcoholic liver injuries.

Description

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Claims

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Application Information

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Owner SOUTH CHINA AGRI UNIV
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