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Anti-inflammatory application and extraction method of 6-hydroxyl-eremophilane-7(11)-en-12,8-lactone in ligularia sibirica

A technology of cucurbit seven and hydroxyl group, applied in the field of natural product chemistry, can solve the problems of low extraction efficiency, no report on anti-inflammatory activity research, etc., and achieve a significant effect of anti-inflammatory activity

Inactive Publication Date: 2015-05-13
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are research reports on the separation of sesquiterpene compound 6-hydroxy-erimorphen-7(11)-en-12,8-lactone from Cucurbit No. 7, but the extraction efficiency is low; No report on the anti-inflammatory activity of 6-hydroxy-erimorphen-7(11)-en-12,8-lactone

Method used

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  • Anti-inflammatory application and extraction method of 6-hydroxyl-eremophilane-7(11)-en-12,8-lactone in ligularia sibirica
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  • Anti-inflammatory application and extraction method of 6-hydroxyl-eremophilane-7(11)-en-12,8-lactone in ligularia sibirica

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Embodiment 1

[0036] Get the dried root and rhizome coarse powder 4kg of Gourd Seven, use the volume fraction as 95% ethanol cold soaking to extract three times, the volume (L) that adds three times is respectively the root of Gourd Seven and the rhizome coarse powder mass (kg) of 6,4 and 4 times, cold soaking for three days each time, stirring three times a day, 10 minutes each time. Filtrate, discard the medicinal residues, combine the extracts, recover the solvent, and obtain 433.0 g of brown ethanol extract.

[0037] After mixing the obtained ethanol extract with diatomaceous earth, first degrease with petroleum ether (60-90 ℃) Soxhlet extraction, remove the strong lipophilic part, then carry out Soxhlet extraction with dichloromethane as solvent for 18 hours, and recover Solvent, concentrated to dichloromethane extract, a total of 160.6g.

[0038] Take 55.0 g of dichloromethane extract, and perform column chromatography on a silica gel column, using petroleum ether (60-90°C)-ethyl aceta...

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Abstract

The invention discloses anti-inflammatory application and an extraction method of 6-hydroxyl-eremophilane-7(11)-en-12,8-lactone in ligularia sibirica, and belongs to the technical field of chemistry of natural products. 6-hydroxyl-eremophilane-7(11)-en-12,8-lactone has an anti-inflammatory effect, thereby being applied to preparation of an anti-inflammatory drug. The extraction method comprises the following steps: 1) taking coarse ligularia sibirica medicinal material powder, and performing cold leaching for extraction with ethanol with the concentration of 95% to obtain an ethanol extract; 2) performing Soxhlet extraction on the obtained extract with petroleum ether (60-90 DEG C) at first, then performing Soxhlet extraction with dichloromethane, and recovering a solvent to obtain a dichloromethane extract; 3) separating the dichloromethane extract through silica gel column chromatography, collecting a petroleum ether (60-90 DEG C)-ethyl acetate eluant, wherein the ratio of petroleum ether (60-90 DEG C) to ethyl acetate is 6:1, carrying out stilling till brown yellow precipitations are separated out, and carrying out refining to obtain a white acicular crystal to be identified as 6-hydroxyl-eremophilane-7(11)-en-12,8-lactone. The extraction method has the advantages of being mild in extraction condition, simple and convenient to operate, high in extraction rate, and low in cost.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to an anti-inflammatory application of 6-hydroxy-erimorphen-7(11)-ene-12,8-lactone in cucurbit seven and an extraction method thereof. Background technique [0002] Traditional Chinese medicine has various chemical components, wide range of biological activities, and has the characteristics of pleiotropy, two-way regulation and small adverse reactions, and has important development and utilization value. Research on the anti-inflammatory activity of active ingredients of traditional Chinese medicine has become a hot spot in the development of new drugs in the world today, and it is also an extremely active research field in modern Chinese medicine. Therefore, the research on the anti-inflammatory active ingredients of traditional Chinese medicine and its mechanism of action is an important way to find anti-inflammatory drugs with high selectivity, stron...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P29/00C07D307/92
Inventor 石娟田英超傅强孟歌刘晶曹胜杨银京郭鹏琪杨云王小娟徐欣雅
Owner XI AN JIAOTONG UNIV
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