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Preparation and application of isoflavone glycoside compound

A technology of isoflavone glycoside compounds and compounds, applied in the field of medicine and natural product chemistry, can solve the problems of no mung bean plant pharmacological activity and active substances

Active Publication Date: 2016-09-21
浅花堂健康科技(中国)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the pharmacological activity and active substances of mung bean plants

Method used

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  • Preparation and application of isoflavone glycoside compound
  • Preparation and application of isoflavone glycoside compound
  • Preparation and application of isoflavone glycoside compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the separation and preparation of 2'-hydroxydaidzin

[0031] 1. Plant material: dried stems and branches of leguminous mung bean (Vigna radiate L.).

[0032] 2. Separation and preparation method: 25 kg of dry stems and branches of mung bean plants, flash extraction twice with 10 or 10 times the amount of 95% ethanol, filter, combine the filtrates, recover the ethanol under reduced pressure until there is no alcohol smell, and obtain about 5 L of concentrated solution; Use 5L of petroleum ether, 5L of dichloromethane, and 5L of n-butanol to extract 3 times each, combine the n-butanol extracts, recover n-butanol under reduced pressure, and obtain the mung bean plant extract; disperse the mung bean plant extract with an appropriate amount of methanol, And take an appropriate amount of silica gel to mix the sample; put the mixed sample into a silica gel column, and use dichloromethane-methanol (100:0~0:100) gradient elution to obtain 6 fractions, fraction 6 [d...

Embodiment 2 2

[0033] Example 2, In Vitro Antioxidative Activity Test of 2′-Hydroxydaidzin

[0034] 1. Instruments: U-1900 spectrophotometer (Hitachi), AB135-S electronic balance (Mettler Toledo), microplate reader (BIO-RAD), pH-2TC (0.01 level) precision digital acidity meter (Shanghai Xin Instrument Co., Ltd.).

[0035] 2. Reagents: sodium chloride (NaCl), potassium chloride (KCl), potassium dihydrogen phosphate (KH 2 PO 4 ), dipotassium hydrogen phosphate (K 2 HPO 4 ), potassium persulfate (K 2 S 2 o 8 ), 2,2'-azino-bis-(3-ethylbenzodihydrothiazoline-6-sulfonic acid) diammonium salt (ABTS), ascorbic acid.

[0036] The 2′-hydroxydaidzin used in the experiment was self-made by the inventor, and its chemical structure was confirmed by hydrogen spectrum, carbon spectrum and mass spectrum, and its HPLC purity was above 98%.

[0037] 3. Experimental method: Prepare 7mmol / L ABTS solution and 2.45mmol / L potassium persulfate solution with phosphate buffered saline solution (PBS) at pH 7.4,...

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Abstract

The invention belongs to the field of medicine and natural product chemistry, and particularly relates to a novel isoflavone glycoside compound and a preparation method and application thereof. The isoflavone glycoside compound has the following structure shown in the description, normal phase (silica gel) column chromatography separation is adopted, reverse (open ODS) column chromatography separation is adopted, and a compound 2'-hydroxyl daidzin is obtained through methanol recrystallization finally. The novel isoflavone glycoside compound can be used for preventing and treating various diseases caused by the fact that too many free radicals in the human body are produced, or the capacity of eliminating the free radicals is decreased. Please see the formula in the description.

Description

technical field [0001] The invention belongs to the field of medicine and natural product chemistry, and specifically relates to a novel isoflavone glycoside compound and its preparation method and application. Background technique [0002] Mung bean is the dry mature seed of the leguminous plant mung bean (Vigna radiate L.), which has the effects of clearing heat, detoxifying and drying dampness. It can be used to treat diarrhea, macule, furuncle, scabies and other diseases. "Materia Medica Huiyan" records that mung beans are used to treat furunculosis, eczema, metal, stone, dan, fire poisons and cholera vomiting. At present, there are no reports on the pharmacological activity and active substances of mung bean plants. [0003] Isoflavone glycosides are ubiquitous in the plant kingdom. The new isoflavone glycosides found in mung bean plants are 2′-hydroxydaidzein, which is a new compound that has not been discovered yet. [0004] In recent years, fillers such as silica ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P9/00A61P25/28A61P35/00A61P17/18
CPCA61K36/48C07H1/08C07H17/07
Inventor 于治国赵云丽白岩
Owner 浅花堂健康科技(中国)有限公司
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