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A kind of preparation method of high-purity epigallocatechin

A technology of gallocatechin and purity table, which is applied in the field of preparing high-content epigallocatechin, can solve the problems of high preparation cost, and achieve the effects of low production cost, simple and convenient operation, and simplified process

Inactive Publication Date: 2011-12-14
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method EGC conversion rate and yield are higher, is suitable for industrialized large-scale production, but the method for preparing EGC with the hydrolysis of Aspergillus oryzae EGCG, the EGC after the hydrolysis and gallic acid also need further separation, and preparation cost is higher

Method used

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  • A kind of preparation method of high-purity epigallocatechin
  • A kind of preparation method of high-purity epigallocatechin
  • A kind of preparation method of high-purity epigallocatechin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Add 100kg of crude tea polyphenol raw material (EGC content is 9.87%) to 500kg of pure water, heat to dissolve, filter after cooling, and the filtrate passes through the macroporous adsorption resin model HP-20 at a flow rate of 0.8 times column volume / hour. Then wash the resin with 8 times the pure water of the column volume; analyze with 15% methanol of 5 times the column volume at a flow rate of 1 column volume / hour, collect the analysis solution, concentrate to 5-30%, and concentrate the solution at 4 Place at ℃ for 24 hours to crystallize; wash the crystals with a small amount of pure water, filter and dry by freeze-drying to obtain powdery EGC. Gained EGC sample carries out purity, and its detection instrument is Agilent1100 high-performance liquid chromatography (Agilent Corporation of the U.S.), and chromatographic column is Shim-pack ODS C18 post (4.6 * 150mm, 5 μ m), mobile phase mobile phase: A phase, water; B Phase, formamide: methanol: glacial acetic acid 4...

Embodiment 2

[0026] Add 100kg of crude tea polyphenol raw material (EGC content is 9.87%) to 5000kg of pure water, heat to dissolve, filter after cooling, and the filtrate passes through the macroporous resin model AB-8 at a flow rate of 1 column volume / hour. Then wash the resin with 10 times the pure water of the column volume; analyze with 5% ethanol 3 times the column volume at a flow rate of 0.8 times the column volume / hour, collect the analysis solution, concentrate to 5-30%, and concentrate the solution at 4 Place at ℃ for 24 hours to crystallize; wash the crystals with a small amount of pure water, filter and dry by freeze-drying to obtain powdery EGC. The EGC content was detected by HPLC to reach 93.24%, and the results are shown in Table 1.

Embodiment 3

[0028] Add 200kg of crude tea polyphenol raw material (EGC content is 9.87%) to 1600kg of pure water, heat to dissolve, filter after cooling, and the filtrate passes through the macroporous resin of model D-101 at a flow rate of 0.8 times column volume / hour. Then wash the resin with 5 times the pure water of the column volume; then analyze with 15% ethanol 2 times the column volume at a flow rate of 0.8 times the column volume / hour, collect the analysis solution, concentrate to 5-30%, and concentrate the solution at 4 Place at ℃ for 24 hours to crystallize; wash the crystals with a small amount of pure water, filter and dry by freeze-drying to obtain powdery EGC. The EGC content was detected by HPLC to reach 92.66%, and the results are shown in Table 1.

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Abstract

The invention provides a method for preparing high-purity epigallocatechin (EGC) from a tea polyphenol rough product, belonging to the technical field of natural product chemistry. According to the method, the tea polyphenol rough product is used as a raw material and is subjected to macroporous resin adsorption, low-concentration aliphatic alcohol elution, concentration, crystallization and drying to obtain the EGC with content of more than 90 percent. The method has the advantages of high content of the EGC obtained by the preparation process, easiness and convenience for operation, low production cost, contribution to industrial production and capability of meeting the market requirements in the fields of modern functional foods, health care products and the like.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a method for preparing high-content epigallocatechin from crude tea polyphenols. Background technique [0002] Tea polyphenols, a polyphenolic component extracted from green tea, are the main active ingredients in tea. Mainly composed of gallocatechin-3-gallate (Epigallocatechin-3-gallate, EGCG), epicatechin-3-gallate (Epicatechin-3-gallate, ECG), epigallocatechin (Epigallocatechin, EGC) and epicatechin Element (Epicatechin, EC) and other components. The content of EGCG and EGC in small-leaf green tea is relatively high, accounting for about 4% and 1% respectively. [0003] There are certain differences in the physiological activities of different tea catechin monomers. EGCG has a good effect on the prevention and treatment of malignant tumors, hyperlipidemia, obesity, neurodegenerative diseases, etc.; ECG has a good antibacterial effect; EGC can s...

Claims

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Application Information

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IPC IPC(8): C07D311/62
Inventor 龚雨顺杨志辉刘仲华黄建安
Owner HUNAN AGRICULTURAL UNIV
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